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The map label for this gene is iaaA [H]

Identifier: 157962256

GI number: 157962256

Start: 2975127

End: 2976212

Strand: Reverse

Name: iaaA [H]

Synonym: Spea_2435

Alternate gene names: 157962256

Gene position: 2976212-2975127 (Counterclockwise)

Preceding gene: 157962259

Following gene: 157962255

Centisome position: 57.52

GC content: 47.05

Gene sequence:

>1086_bases
ATGCTCCGTAGTACAGTCTTGCTTGTTTTATCGTCACTCATTTCGACAGGTACCATGGCCAGTGATAAGCCGTTTTCAAT
TGCGATTCATGGTGGCGCAGGCACCATCTCTAAGGCTAATTTGACACCCGAGCAGCAAAAATCTTATCGCGACAAACTCC
AACAAGCCGTCGACGCGGGTTACGATATTCTCGCAAAAGGGGGGGACAGCTTATCAGCCGTTCGTGCATCGATTAATATC
CTAGAAAATAGTCCGCTATTTAATGCGGGGCTCGGCGCTGTGTATACCTATGATGGCAGCCACGAGATGGACGCGTCGAT
TATGGATGGAAAAACCATGAATGCCGGAGCGGTGGCCGGAGTGAAACATATTAAAAATCCAATTGATTTAGCAATGACTG
TAATGGAAAAATCTCCTCATGTGATGCTATCTGGCCAAGGTGCAGAGGAATTTGCACTGACTCAGGGTTTTCAATTAGTG
CCAGTTAATTCATTTGATACTGAAAGCCGTTATCAGCAGTTAAAGAGTGCTAAAGAGAAAATTCAAAAAGCGGAGTCGAG
CCCTGAATATCAAGCAAGCACAGGATTGAGTACAGGCTCTAGTACTGGCCCTAATTCTCACCATAATTATAAACAAAGTG
CCCTCGATTATAGCGAGTTTGACTATAAGTTCGGTACTGTGGGCGCAGTAGCCCTAGATCAAAATGGTAATTTAGCGGCA
GGAACCTCAACTGGGGGCATGACGGCAAAGCGATTTGGCCGCATTGGAGACTCGCCCATTATAGGTGCTGGCACCTATGC
CGAGAACGGTGTTTGCGCCGTGTCGGCAACTGGCCACGGAGAGTACTTTATTCGCTACCATGTGGCGGGGGATATTTGCG
CTAAGGTGAAATATCAGAAGAAATCGATTATTCAAGCAGCCGATGAAGTGATTAATCAACGGCTTATTACGGCTGGTGGC
ACTGGCGGTGTGATCGCCATCGATCAACGCGGCAATATCGCCACGCCCTTTAACACTGAGGGCATGTACCGAGCTACACG
AAAAAATAATGAACCTGCGCAAGTGATGATCTGGTCAGATTTATAA

Upstream 100 bases:

>100_bases
AGAATCGTTAACTCAAGCAAGTTGCTGATTGTAATTATGGCCGAAAATGGTTACAAAGAGAGCAAATAAATACAATAGGA
TACAGTTTGTGAAGTATACG

Downstream 100 bases:

>100_bases
CCAACTTGTGAACATTCATTTTACTCACTTTATCTATGACGACGCGCACTTAAGTTCACTATTCTGAGCTTGGTGCGCTT
TTACACGCTTTAGTATTTTG

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MLRSTVLLVLSSLISTGTMASDKPFSIAIHGGAGTISKANLTPEQQKSYRDKLQQAVDAGYDILAKGGDSLSAVRASINI
LENSPLFNAGLGAVYTYDGSHEMDASIMDGKTMNAGAVAGVKHIKNPIDLAMTVMEKSPHVMLSGQGAEEFALTQGFQLV
PVNSFDTESRYQQLKSAKEKIQKAESSPEYQASTGLSTGSSTGPNSHHNYKQSALDYSEFDYKFGTVGAVALDQNGNLAA
GTSTGGMTAKRFGRIGDSPIIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQKKSIIQAADEVINQRLITAGG
TGGVIAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSDL

Sequences:

>Translated_361_residues
MLRSTVLLVLSSLISTGTMASDKPFSIAIHGGAGTISKANLTPEQQKSYRDKLQQAVDAGYDILAKGGDSLSAVRASINI
LENSPLFNAGLGAVYTYDGSHEMDASIMDGKTMNAGAVAGVKHIKNPIDLAMTVMEKSPHVMLSGQGAEEFALTQGFQLV
PVNSFDTESRYQQLKSAKEKIQKAESSPEYQASTGLSTGSSTGPNSHHNYKQSALDYSEFDYKFGTVGAVALDQNGNLAA
GTSTGGMTAKRFGRIGDSPIIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQKKSIIQAADEVINQRLITAGG
TGGVIAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSDL
>Mature_361_residues
MLRSTVLLVLSSLISTGTMASDKPFSIAIHGGAGTISKANLTPEQQKSYRDKLQQAVDAGYDILAKGGDSLSAVRASINI
LENSPLFNAGLGAVYTYDGSHEMDASIMDGKTMNAGAVAGVKHIKNPIDLAMTVMEKSPHVMLSGQGAEEFALTQGFQLV
PVNSFDTESRYQQLKSAKEKIQKAESSPEYQASTGLSTGSSTGPNSHHNYKQSALDYSEFDYKFGTVGAVALDQNGNLAA
GTSTGGMTAKRFGRIGDSPIIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQKKSIIQAADEVINQRLITAGG
TGGVIAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSDL

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=328, Percent_Identity=37.1951219512195, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI145275200, Length=328, Percent_Identity=37.1951219512195, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI98991769, Length=333, Percent_Identity=30.6306306306306, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI285002251, Length=243, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI285002253, Length=243, Percent_Identity=31.6872427983539, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787050, Length=319, Percent_Identity=48.2758620689655, Blast_Score=290, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI17569239, Length=247, Percent_Identity=29.1497975708502, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI18921183, Length=321, Percent_Identity=34.2679127725857, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI21357893, Length=279, Percent_Identity=33.6917562724014, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI221330102, Length=245, Percent_Identity=34.2857142857143, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24652135, Length=245, Percent_Identity=34.2857142857143, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24655553, Length=226, Percent_Identity=31.858407079646, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24657358, Length=240, Percent_Identity=27.0833333333333, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 38238; Mature: 38238

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSTVLLVLSSLISTGTMASDKPFSIAIHGGAGTISKANLTPEQQKSYRDKLQQAVDAG
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCC
YDILAKGGDSLSAVRASINILENSPLFNAGLGAVYTYDGSHEMDASIMDGKTMNAGAVAG
CEEEECCCCHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCHHHH
VKHIKNPIDLAMTVMEKSPHVMLSGQGAEEFALTQGFQLVPVNSFDTESRYQQLKSAKEK
HHHHCCHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
IQKAESSPEYQASTGLSTGSSTGPNSHHNYKQSALDYSEFDYKFGTVGAVALDQNGNLAA
HHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCHHHCCEECCCEEEEEECCCCCEEE
GTSTGGMTAKRFGRIGDSPIIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQK
CCCCCCCHHHHHCCCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHH
KSIIQAADEVINQRLITAGGTGGVIAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSD
HHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEHHCCCCCCCEEEEEEC
L
C
>Mature Secondary Structure
MLRSTVLLVLSSLISTGTMASDKPFSIAIHGGAGTISKANLTPEQQKSYRDKLQQAVDAG
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCC
YDILAKGGDSLSAVRASINILENSPLFNAGLGAVYTYDGSHEMDASIMDGKTMNAGAVAG
CEEEECCCCHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCHHHH
VKHIKNPIDLAMTVMEKSPHVMLSGQGAEEFALTQGFQLVPVNSFDTESRYQQLKSAKEK
HHHHCCHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
IQKAESSPEYQASTGLSTGSSTGPNSHHNYKQSALDYSEFDYKFGTVGAVALDQNGNLAA
HHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCHHHCCEECCCEEEEEECCCCCEEE
GTSTGGMTAKRFGRIGDSPIIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQK
CCCCCCCHHHHHCCCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHH
KSIIQAADEVINQRLITAGGTGGVIAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSD
HHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEHHCCCCCCCEEEEEEC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]