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The map label for this gene is napA [H]

Identifier: 157962248

GI number: 157962248

Start: 2964400

End: 2966889

Strand: Reverse

Name: napA [H]

Synonym: Spea_2427

Alternate gene names: 157962248

Gene position: 2966889-2964400 (Counterclockwise)

Preceding gene: 157962249

Following gene: 157962247

Centisome position: 57.34

GC content: 47.95

Gene sequence:

>2490_bases
ATGAACAGACGCGAATTTATGAAGGCCAACGCTGCTTTGACTGCAGCGAGTGTCGCTGGCCTAGCCTTACCCGCTACCGC
GAGTAACCTGATCACCAGTTCTGAAGAAACAAAACTAGAATGGAACAAGGCGCCGTGTCGTTTTTGCGGTACAGGTTGCT
CGGTGATTGTTGCGACACGCGACGGCAAAGTGGTTGCAACACATGGTGATGCAGAAAGTGAAGTGAACAAGGGCCTTAAC
TGTATCAAGGGCTACTTCCTATCTAAGATCATGTACGGTAAAGATAGACTTCAAACCCCAATGCTACGTATGACAGACGG
TAAGTATGACAAGAACGGTGAGTTTACTTCCGTATCTTGGGATACCGCGTTTGATACTATGGCGCAAAAGTGGAAGAAAA
CCATTAAAGACAAAGGACCAACCGCAATTGGTATGTTTGGTTCTGGTCAGTGGACTGTTTGGGAAGGCTACGCGGCTTCA
AAACTGATGAAAGCGGGCTTTGGTTCAAACAATATCGACCCTAACGCTCGTCACTGTATGGCCTCTGCCGTAGGTGGCTT
TATGCGTACCTTCGGTATCGACGAGCCAATGGGTTGCTATGATGATATGGAAGCGGCCGATGCATTCGTGCTTTGGGGCT
CTAATATGGCAGAGATGCACCCGATCCTTTGGAGCCGTGTGACAGATCGCCGCTTAAGCGCCCCTCATGTGAATGTGGCA
GTTCTTTCGACATTTGAACATAGATCGTTCGACCTTGCCGATTTAGGCATCGTATTTACGCCACAGACTGATTTAGCAAT
GCTGAACTTCGTTGCAAACTACATCATTCAAAACGATAAAGTGAATTGGGACTTTGTTAACAAACACGTTAACTTCCGCA
AAGGTGAAACCGATATCGGTTATGGCCTGCGTCCATCTCATCCGCTACAAAAAGCCGCTAAGAACGTCAAAACAGCCGGC
GACTCAACACCTATCGACTTTGAGGCATTTAAGAAGTTTGTTGCCGATTACGACGTGAAGTCTGTTAGCAAGCTTTCTGG
GGTACCAGAACATAAGCTAATAGAGCTGGCTGAGCTTTATGCCGATCCAAATAAGAAAGTAACCTCTTTCTGGACCATGG
GCTTTAATCAGCATACTCGTGGTGTGTGGTGTAACAACCTTATCTACAACATTCACTTGTTAACGGGTAAGATTTCGACT
CCGGGTAACAGCCCATTCTCTCTTACTGGTCAGCCTTCGGCTTGTGGTACTGCACGTGAAGTGGGAACCTTCTCACACCG
TCTGCCTGCAGACATGGTGGTTAAAAATCCAAAGCATAGAGCGATTGCTGAAAAGATTTGGCGTATTCCAAGTGGCATTA
TTCCACCAAAGCCTGGTTATCACGCAGTTGAGCAAAACCGTCGTCTAAAAGATGGTGACATCAATGCCTATTGGGTTCAG
GTGAACAACAATATGCAGGCCGGTCCTAATATCAACGAAGAAGGCTTGCCGGGTTATCGCAACCCTGAAAACTTCATCGT
GGTATCAGATGCTTACCCAACAGTAACCACGCAGGCTGCGGACCTTATTCTGCCAACCGCGATGTGGGTAGAGAAAGAGG
GCGCTTATGGTAACGCCGAGCGTCGTACCCAATTCTGGCACCAGATGGTGAAAGCGCCGGGTGAGTCTCGCTCAGATCTT
TGGCAGCTAATGGAGTTCTCAAAGCGCTTCACTACTGACGAAGTTTGGCCTGCCGATGTACTTGCTGCAAACCCTGAATT
TAAAGGTAAGACACTGTTTGAAGTGCTGTATCAAAACGGCAACGTGGAGAAATTCCCACTGTCAGACAGAAACAGTAAAT
ACATGAATGACGAAGCTGAAGACTTTGGCTTCTATGTTCAAAAAGGTCTATTTGAGGAGTATGCCACCTTCGGTCGTGGT
CATGCCCATGATTTGGCACCATTTGATACTTACCATGAGGTTCACGGCCTACGCTGGCCTGTTGTGGATGGCAAAGAAAC
TAAATGGCGCTTTAAAGAAGGTAGTGATCCCTATGTGAAGGCGGGTTCTGGTTTTGAGTTCTACGGTAAGCCAGACGGTA
AAGCGGTTATTTTTGCACTGCCATACGAGCCAGCTGCTGAGTCGCCAGATGATGAGTTTGATATTTGGTTATCGACGGGG
CGTGTACTGGAACATTGGCATTCAGGCTCAATGACGCAGCGCGTACCTGAGCTATATCGAGCCTTCCCTGATGCTGTGTG
TTTTATGCATCCACAAGATGCCAAACAGCGAGGCTTACGCCGCGGTGATGAAGTACGGGTGATTTCTCGCCGAGGTGAAA
TTAAGACCCGAGTCGAAACTCGTGGTCGTAACAAGCCGCCAGTGGGATTAGTGTTCGTGCCTTGGTTCGATGCGAGTCAG
CTGATTAATAAGGTGACTCTCGATGCAACCGATCCACTGTCTAAGCAGACCGACTTTAAGAAGTGCGCAGTGAAGATCGT
CAAGGCATAA

Upstream 100 bases:

>100_bases
TAATGACAAGTATCGAAACTATTAATGCCATACCAGGAGTGTTGTCGGCTGCAATGGTTTATCACCAAAGTGAAGTGCTT
GAAGAGGGTGAACAATGAAA

Downstream 100 bases:

>100_bases
GGAGAAATAATAATGAAAACATTAACACGAGTATCTGCAGCTGCTTTGATGAGCTTATCTTTAGCAAGTGCTTTTAGTGT
TTCAGCAGAGGTTATTCCAG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MNRREFMKANAALTAASVAGLALPATASNLITSSEETKLEWNKAPCRFCGTGCSVIVATRDGKVVATHGDAESEVNKGLN
CIKGYFLSKIMYGKDRLQTPMLRMTDGKYDKNGEFTSVSWDTAFDTMAQKWKKTIKDKGPTAIGMFGSGQWTVWEGYAAS
KLMKAGFGSNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVNVA
VLSTFEHRSFDLADLGIVFTPQTDLAMLNFVANYIIQNDKVNWDFVNKHVNFRKGETDIGYGLRPSHPLQKAAKNVKTAG
DSTPIDFEAFKKFVADYDVKSVSKLSGVPEHKLIELAELYADPNKKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVKNPKHRAIAEKIWRIPSGIIPPKPGYHAVEQNRRLKDGDINAYWVQ
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVKAPGESRSDL
WQLMEFSKRFTTDEVWPADVLAANPEFKGKTLFEVLYQNGNVEKFPLSDRNSKYMNDEAEDFGFYVQKGLFEEYATFGRG
HAHDLAPFDTYHEVHGLRWPVVDGKETKWRFKEGSDPYVKAGSGFEFYGKPDGKAVIFALPYEPAAESPDDEFDIWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPQDAKQRGLRRGDEVRVISRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKA

Sequences:

>Translated_829_residues
MNRREFMKANAALTAASVAGLALPATASNLITSSEETKLEWNKAPCRFCGTGCSVIVATRDGKVVATHGDAESEVNKGLN
CIKGYFLSKIMYGKDRLQTPMLRMTDGKYDKNGEFTSVSWDTAFDTMAQKWKKTIKDKGPTAIGMFGSGQWTVWEGYAAS
KLMKAGFGSNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVNVA
VLSTFEHRSFDLADLGIVFTPQTDLAMLNFVANYIIQNDKVNWDFVNKHVNFRKGETDIGYGLRPSHPLQKAAKNVKTAG
DSTPIDFEAFKKFVADYDVKSVSKLSGVPEHKLIELAELYADPNKKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVKNPKHRAIAEKIWRIPSGIIPPKPGYHAVEQNRRLKDGDINAYWVQ
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVKAPGESRSDL
WQLMEFSKRFTTDEVWPADVLAANPEFKGKTLFEVLYQNGNVEKFPLSDRNSKYMNDEAEDFGFYVQKGLFEEYATFGRG
HAHDLAPFDTYHEVHGLRWPVVDGKETKWRFKEGSDPYVKAGSGFEFYGKPDGKAVIFALPYEPAAESPDDEFDIWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPQDAKQRGLRRGDEVRVISRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKA
>Mature_829_residues
MNRREFMKANAALTAASVAGLALPATASNLITSSEETKLEWNKAPCRFCGTGCSVIVATRDGKVVATHGDAESEVNKGLN
CIKGYFLSKIMYGKDRLQTPMLRMTDGKYDKNGEFTSVSWDTAFDTMAQKWKKTIKDKGPTAIGMFGSGQWTVWEGYAAS
KLMKAGFGSNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVNVA
VLSTFEHRSFDLADLGIVFTPQTDLAMLNFVANYIIQNDKVNWDFVNKHVNFRKGETDIGYGLRPSHPLQKAAKNVKTAG
DSTPIDFEAFKKFVADYDVKSVSKLSGVPEHKLIELAELYADPNKKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVKNPKHRAIAEKIWRIPSGIIPPKPGYHAVEQNRRLKDGDINAYWVQ
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVKAPGESRSDL
WQLMEFSKRFTTDEVWPADVLAANPEFKGKTLFEVLYQNGNVEKFPLSDRNSKYMNDEAEDFGFYVQKGLFEEYATFGRG
HAHDLAPFDTYHEVHGLRWPVVDGKETKWRFKEGSDPYVKAGSGFEFYGKPDGKAVIFALPYEPAAESPDDEFDIWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPQDAKQRGLRRGDEVRVISRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=828, Percent_Identity=71.0144927536232, Blast_Score=1275, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=793, Percent_Identity=24.4640605296343, Blast_Score=212, Evalue=8e-56,
Organism=Escherichia coli, GI3868720, Length=660, Percent_Identity=21.969696969697, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787870, Length=833, Percent_Identity=22.5690276110444, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI87081797, Length=885, Percent_Identity=23.3898305084746, Blast_Score=98, Evalue=3e-21,
Organism=Escherichia coli, GI3868719, Length=662, Percent_Identity=21.1480362537764, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI171474008, Length=885, Percent_Identity=22.5988700564972, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92906; Mature: 92906

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRREFMKANAALTAASVAGLALPATASNLITSSEETKLEWNKAPCRFCGTGCSVIVATR
CCHHHHHHHHHHHHHHHHHCEECCCCHHHHHCCCCCCEEECCCCCCCEECCCCEEEEEEC
DGKVVATHGDAESEVNKGLNCIKGYFLSKIMYGKDRLQTPMLRMTDGKYDKNGEFTSVSW
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEECCCCCCCCCCEEEEEC
DTAFDTMAQKWKKTIKDKGPTAIGMFGSGQWTVWEGYAASKLMKAGFGSNNIDPNARHCM
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHHHHCCCCCCCCCCHHHHH
ASAVGGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVNVA
HHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEE
VLSTFEHRSFDLADLGIVFTPQTDLAMLNFVANYIIQNDKVNWDFVNKHVNFRKGETDIG
EEEECCCCCCCHHHCCEEECCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCC
YGLRPSHPLQKAAKNVKTAGDSTPIDFEAFKKFVADYDVKSVSKLSGVPEHKLIELAELY
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHH
ADPNKKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTARE
CCCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCHHH
VGTFSHRLPADMVVKNPKHRAIAEKIWRIPSGIIPPKPGYHAVEQNRRLKDGDINAYWVQ
HCCHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEE
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQMVKAPGESRSDLWQLMEFSKRFTTDEVWPADVLAANPEFKGKTLFEVLYQNG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCHHHHHHHHCC
NVEKFPLSDRNSKYMNDEAEDFGFYVQKGLFEEYATFGRGHAHDLAPFDTYHEVHGLRWP
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCC
VVDGKETKWRFKEGSDPYVKAGSGFEFYGKPDGKAVIFALPYEPAAESPDDEFDIWLSTG
CCCCCCCCEEECCCCCCEEECCCCCEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEECC
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPQDAKQRGLRRGDEVRVISRRGEIKTRVET
HHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCEEEEECCCCEEEHHHH
RGRNKPPVGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKIVKA
CCCCCCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNRREFMKANAALTAASVAGLALPATASNLITSSEETKLEWNKAPCRFCGTGCSVIVATR
CCHHHHHHHHHHHHHHHHHCEECCCCHHHHHCCCCCCEEECCCCCCCEECCCCEEEEEEC
DGKVVATHGDAESEVNKGLNCIKGYFLSKIMYGKDRLQTPMLRMTDGKYDKNGEFTSVSW
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEECCCCCCCCCCEEEEEC
DTAFDTMAQKWKKTIKDKGPTAIGMFGSGQWTVWEGYAASKLMKAGFGSNNIDPNARHCM
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHHHHCCCCCCCCCCHHHHH
ASAVGGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVNVA
HHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEE
VLSTFEHRSFDLADLGIVFTPQTDLAMLNFVANYIIQNDKVNWDFVNKHVNFRKGETDIG
EEEECCCCCCCHHHCCEEECCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCC
YGLRPSHPLQKAAKNVKTAGDSTPIDFEAFKKFVADYDVKSVSKLSGVPEHKLIELAELY
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHH
ADPNKKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTARE
CCCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCHHH
VGTFSHRLPADMVVKNPKHRAIAEKIWRIPSGIIPPKPGYHAVEQNRRLKDGDINAYWVQ
HCCHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEE
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQMVKAPGESRSDLWQLMEFSKRFTTDEVWPADVLAANPEFKGKTLFEVLYQNG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCHHHHHHHHCC
NVEKFPLSDRNSKYMNDEAEDFGFYVQKGLFEEYATFGRGHAHDLAPFDTYHEVHGLRWP
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCC
VVDGKETKWRFKEGSDPYVKAGSGFEFYGKPDGKAVIFALPYEPAAESPDDEFDIWLSTG
CCCCCCCCEEECCCCCCEEECCCCCEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEECC
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPQDAKQRGLRRGDEVRVISRRGEIKTRVET
HHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCEEEEECCCCEEEHHHH
RGRNKPPVGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKIVKA
CCCCCCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA