Click here to switch to the map view.

The map label for this gene is napB [H]

Identifier: 157962247

GI number: 157962247

Start: 2963902

End: 2964387

Strand: Reverse

Name: napB [H]

Synonym: Spea_2426

Alternate gene names: 157962247

Gene position: 2964387-2963902 (Counterclockwise)

Preceding gene: 157962248

Following gene: 157962246

Centisome position: 57.29

GC content: 46.09

Gene sequence:

>486_bases
ATGAAAACATTAACACGAGTATCTGCAGCTGCTTTGATGAGCTTATCTTTAGCAAGTGCTTTTAGTGTTTCAGCAGAGGT
TATTCCAGCTGACAAGATAGCGACGCTACGTGAGGCTCCGCTGAACGTTGAGCCAACGCCACCTGCGATGCAAAAAGTGA
TTAACTCTGATGTAAAAGAAGTGCGTAACTACCCGATGCAACCACCAGTGATCCCCCATAAGATTGATGGCTATCAGGTT
AACTTAAAGGTTAATAAGTGTATGTCTTGTCACGACCGTAGCCGCACGGGTGAGTCACAAGCACCTATGGTGAGCGTGAC
TCACTATATGGACCGCGAGAACAACTTCCTTGCGACCCTGTCTCCAAGACGTTACTTCTGTACCCAATGCCACGTGCCAC
AACTAGACGCTAAAATTCTAGTGCCGAATGAGTTTGTGGATATGGACCAGCTAATGAAAGCCGATTCTAATAAGAAAGCA
CACTAG

Upstream 100 bases:

>100_bases
GATTAATAAGGTGACTCTCGATGCAACCGATCCACTGTCTAAGCAGACCGACTTTAAGAAGTGCGCAGTGAAGATCGTCA
AGGCATAAGGAGAAATAATA

Downstream 100 bases:

>100_bases
GAGTAGTTATGATCGCTAAATTACGATCTTTCCTTAAAAAGGTATGGACAGTGCTGAGAAAGCCAAGTGTTCATTACAGC
TTAGGCTTTTTAACGCTCGG

Product: nitrate reductase cytochrome c-type subunit NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKTLTRVSAAALMSLSLASAFSVSAEVIPADKIATLREAPLNVEPTPPAMQKVINSDVKEVRNYPMQPPVIPHKIDGYQV
NLKVNKCMSCHDRSRTGESQAPMVSVTHYMDRENNFLATLSPRRYFCTQCHVPQLDAKILVPNEFVDMDQLMKADSNKKA
H

Sequences:

>Translated_161_residues
MKTLTRVSAAALMSLSLASAFSVSAEVIPADKIATLREAPLNVEPTPPAMQKVINSDVKEVRNYPMQPPVIPHKIDGYQV
NLKVNKCMSCHDRSRTGESQAPMVSVTHYMDRENNFLATLSPRRYFCTQCHVPQLDAKILVPNEFVDMDQLMKADSNKKA
H
>Mature_161_residues
MKTLTRVSAAALMSLSLASAFSVSAEVIPADKIATLREAPLNVEPTPPAMQKVINSDVKEVRNYPMQPPVIPHKIDGYQV
NLKVNKCMSCHDRSRTGESQAPMVSVTHYMDRENNFLATLSPRRYFCTQCHVPQLDAKILVPNEFVDMDQLMKADSNKKA
H

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=84, Percent_Identity=47.6190476190476, Blast_Score=97, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 17969; Mature: 17969

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTRVSAAALMSLSLASAFSVSAEVIPADKIATLREAPLNVEPTPPAMQKVINSDVKE
CCCHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VRNYPMQPPVIPHKIDGYQVNLKVNKCMSCHDRSRTGESQAPMVSVTHYMDRENNFLATL
HHCCCCCCCCCCEECCCEEEEEEHHHHHCHHCCCCCCCCCCCEEEEHHHHCCCCCEEEEE
SPRRYFCTQCHVPQLDAKILVPNEFVDMDQLMKADSNKKAH
CCCCEECCCCCCCCCCCEEECCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTLTRVSAAALMSLSLASAFSVSAEVIPADKIATLREAPLNVEPTPPAMQKVINSDVKE
CCCHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VRNYPMQPPVIPHKIDGYQVNLKVNKCMSCHDRSRTGESQAPMVSVTHYMDRENNFLATL
HHCCCCCCCCCCEECCCEEEEEEHHHHHCHHCCCCCCCCCCCEEEEHHHHCCCCCEEEEE
SPRRYFCTQCHVPQLDAKILVPNEFVDMDQLMKADSNKKAH
CCCCEECCCCCCCCCCCEEECCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]