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The map label for this gene is eptA [H]

Identifier: 157962147

GI number: 157962147

Start: 2834856

End: 2836499

Strand: Reverse

Name: eptA [H]

Synonym: Spea_2326

Alternate gene names: 157962147

Gene position: 2836499-2834856 (Counterclockwise)

Preceding gene: 157962148

Following gene: 157962146

Centisome position: 54.82

GC content: 41.91

Gene sequence:

>1644_bases
GTGTTATCACGTCTTAGAAATCTAAATAGTATTCAATTCACACTTATACTTGCGATTTATTATGTGTGCGTACTTAATAT
TCCGCTTTTTCAAATTATCAAACAGGGCGTGGATAAACAAGCCGATGTGAATATGGCCTTTATTGCGACCATGCCTTTTT
TTCTGTTATTTGCACTGTCTTTTTTATTTAGTATTTTTAGTGTGAAGTACCTGGCAAAGCCATTCTTCATCATTCTGACC
CTGATATCATCGAGTGTGTTTTTTGCCGCTTTTCAATATGGAGTGGTTTTTGACTACGGCATGATTGAAAACACCGTTCA
GAGCAATCAATCGGAGGCGGCGACCTACCTTAATTGGGCATCTGTCCTCAATTTCGCTGTGACGGGGCTAATACCTTGCT
TGCTTATATATAAAGCCAATATTCAATACAAACCCTTTAGTAAGGAGTTACTGCATAAGGCCGTGTTTATGCTGTCTATG
CTCGCGGGCATAGTGGTGATAGGCATGTTCTATTATCAAAACTACGTCTCTTTTGGCCGTAATAACGACATCATTAAGCG
TTATGTTATTCCTACCTATTTTATTGGTTCTACCGCCAAGTACATTGATGTCAACTATCTGCAACAGCCGCTTGAGTATA
AACAGCTTGGCCTAGATGCGAAAAATACCACGGCTAAAACTAATTCTAAAACCCATCCGCAAACTAAGCCTAATTTAGTG
GTGCTGGTGGTCGGCGAAACGGCAAGAGCGGCTAATTATCATTACTATGGTTATGACAGACCAACCAATGCGCATACCAA
AGTTCAAGGCTTAATCGCATTTCAAGATACGCACTCTTGTGGCACTGCAACCGCAGTCTCTTTACCTTGCATGTTTTCAA
GAATGGATCATAAAAATTACGACTCTCGTCGTGCAATGGCTCAAGATAATGTCATCGACATACTGAATCATGCAGGCATT
CAACTCGAATGGCTCGATAATGACAGCGGCTGTAAAGGGGTTTGTCAGAACATAGAGCATGTGGTTATCGACCATAACAG
CGATCCCAAGCTATGCAACGGCGAATTTTGTTATGACCAAGTCTTAATAAATGAGCTGGATAAACGCCTTAAGACGATTG
AGCGAAAAGACACCTTGCTGGTGCTGCATATCATTGGCTCCCATGGGCCGACATACTATCTGCGTTACCCTGAGCAACAC
CGGCGCTTTGTACCTGACTGTCAGAGAAGTGATATTCAAAACTGTACTCACCAACAGCTGGTTAACACCTATGACAATAC
GATTTTGTACACAGATTATATTGTGTCACAGGTGGTCAAAGAACTAGAGGGGGAGAGTAAGCAATTTGACACTGCAATGC
TTTATATCTCGGATCACGGTGAGTCATTAGGGGAAAGCGGTATGTATCTTCATGGTACGCCGTATTCATTTGCGCCAGGT
GAGCAGACTAAGGTGCCTTTATTAGCTTGGATCTCTAACGGATTCGCCGAACAAAATCGTCTGGATCTAACCTGCCTAAA
TAAAGAAGCGGCTAAGGGAGGTTTTTCCCATGATAACTTGTTCGATAGCTTACTTGGCTTGATGAATGTGAAAACCAGTA
TATATCGCCCAAGTACCGATATTTTCTTGGGTTGTAGGAAATAA

Upstream 100 bases:

>100_bases
AGCCATTTTGTTAAGGTGCTATTATAAAGCCATCCCCCCCGATAGCGGGCTATAGGCAAGGAGAGCCATAAATAACCTTA
AAAATAAGTGAGTAAACTCA

Downstream 100 bases:

>100_bases
ATAGTCATTAATCAGTAAATTTATTTGAAAAGGGGGTGGCAAGCCCCTTTTTTTTGTTTAAAGTATTGATAATCATGGAA
ATCGATCGAGAGGCGTCAAT

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MLSRLRNLNSIQFTLILAIYYVCVLNIPLFQIIKQGVDKQADVNMAFIATMPFFLLFALSFLFSIFSVKYLAKPFFIILT
LISSSVFFAAFQYGVVFDYGMIENTVQSNQSEAATYLNWASVLNFAVTGLIPCLLIYKANIQYKPFSKELLHKAVFMLSM
LAGIVVIGMFYYQNYVSFGRNNDIIKRYVIPTYFIGSTAKYIDVNYLQQPLEYKQLGLDAKNTTAKTNSKTHPQTKPNLV
VLVVGETARAANYHYYGYDRPTNAHTKVQGLIAFQDTHSCGTATAVSLPCMFSRMDHKNYDSRRAMAQDNVIDILNHAGI
QLEWLDNDSGCKGVCQNIEHVVIDHNSDPKLCNGEFCYDQVLINELDKRLKTIERKDTLLVLHIIGSHGPTYYLRYPEQH
RRFVPDCQRSDIQNCTHQQLVNTYDNTILYTDYIVSQVVKELEGESKQFDTAMLYISDHGESLGESGMYLHGTPYSFAPG
EQTKVPLLAWISNGFAEQNRLDLTCLNKEAAKGGFSHDNLFDSLLGLMNVKTSIYRPSTDIFLGCRK

Sequences:

>Translated_547_residues
MLSRLRNLNSIQFTLILAIYYVCVLNIPLFQIIKQGVDKQADVNMAFIATMPFFLLFALSFLFSIFSVKYLAKPFFIILT
LISSSVFFAAFQYGVVFDYGMIENTVQSNQSEAATYLNWASVLNFAVTGLIPCLLIYKANIQYKPFSKELLHKAVFMLSM
LAGIVVIGMFYYQNYVSFGRNNDIIKRYVIPTYFIGSTAKYIDVNYLQQPLEYKQLGLDAKNTTAKTNSKTHPQTKPNLV
VLVVGETARAANYHYYGYDRPTNAHTKVQGLIAFQDTHSCGTATAVSLPCMFSRMDHKNYDSRRAMAQDNVIDILNHAGI
QLEWLDNDSGCKGVCQNIEHVVIDHNSDPKLCNGEFCYDQVLINELDKRLKTIERKDTLLVLHIIGSHGPTYYLRYPEQH
RRFVPDCQRSDIQNCTHQQLVNTYDNTILYTDYIVSQVVKELEGESKQFDTAMLYISDHGESLGESGMYLHGTPYSFAPG
EQTKVPLLAWISNGFAEQNRLDLTCLNKEAAKGGFSHDNLFDSLLGLMNVKTSIYRPSTDIFLGCRK
>Mature_547_residues
MLSRLRNLNSIQFTLILAIYYVCVLNIPLFQIIKQGVDKQADVNMAFIATMPFFLLFALSFLFSIFSVKYLAKPFFIILT
LISSSVFFAAFQYGVVFDYGMIENTVQSNQSEAATYLNWASVLNFAVTGLIPCLLIYKANIQYKPFSKELLHKAVFMLSM
LAGIVVIGMFYYQNYVSFGRNNDIIKRYVIPTYFIGSTAKYIDVNYLQQPLEYKQLGLDAKNTTAKTNSKTHPQTKPNLV
VLVVGETARAANYHYYGYDRPTNAHTKVQGLIAFQDTHSCGTATAVSLPCMFSRMDHKNYDSRRAMAQDNVIDILNHAGI
QLEWLDNDSGCKGVCQNIEHVVIDHNSDPKLCNGEFCYDQVLINELDKRLKTIERKDTLLVLHIIGSHGPTYYLRYPEQH
RRFVPDCQRSDIQNCTHQQLVNTYDNTILYTDYIVSQVVKELEGESKQFDTAMLYISDHGESLGESGMYLHGTPYSFAPG
EQTKVPLLAWISNGFAEQNRLDLTCLNKEAAKGGFSHDNLFDSLLGLMNVKTSIYRPSTDIFLGCRK

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=533, Percent_Identity=36.7729831144465, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI87082286, Length=458, Percent_Identity=25.764192139738, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87082223, Length=452, Percent_Identity=23.6725663716814, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790392, Length=471, Percent_Identity=21.0191082802548, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 62087; Mature: 62087

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRLRNLNSIQFTLILAIYYVCVLNIPLFQIIKQGVDKQADVNMAFIATMPFFLLFALS
CCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
FLFSIFSVKYLAKPFFIILTLISSSVFFAAFQYGVVFDYGMIENTVQSNQSEAATYLNWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHHH
SVLNFAVTGLIPCLLIYKANIQYKPFSKELLHKAVFMLSMLAGIVVIGMFYYQNYVSFGR
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NNDIIKRYVIPTYFIGSTAKYIDVNYLQQPLEYKQLGLDAKNTTAKTNSKTHPQTKPNLV
CCCHHHHHCCCEEEECCCCCEEEHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
VLVVGETARAANYHYYGYDRPTNAHTKVQGLIAFQDTHSCGTATAVSLPCMFSRMDHKNY
EEEECCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
DSRRAMAQDNVIDILNHAGIQLEWLDNDSGCKGVCQNIEHVVIDHNSDPKLCNGEFCYDQ
CHHHHHHHCCHHHHHHCCCCEEEECCCCCCCHHHHCCCCEEEEECCCCCCCCCCCHHHHH
VLINELDKRLKTIERKDTLLVLHIIGSHGPTYYLRYPEQHRRFVPDCQRSDIQNCTHQQL
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHH
VNTYDNTILYTDYIVSQVVKELEGESKQFDTAMLYISDHGESLGESGMYLHGTPYSFAPG
HHHHCCEEEEHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCCC
EQTKVPLLAWISNGFAEQNRLDLTCLNKEAAKGGFSHDNLFDSLLGLMNVKTSIYRPSTD
CCCCCHHHEEHHCCCCCCCCCEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
IFLGCRK
EEEEECC
>Mature Secondary Structure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EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]