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The map label for this gene is pleD [H]

Identifier: 157962146

GI number: 157962146

Start: 2833701

End: 2834753

Strand: Reverse

Name: pleD [H]

Synonym: Spea_2325

Alternate gene names: 157962146

Gene position: 2834753-2833701 (Counterclockwise)

Preceding gene: 157962147

Following gene: 157962144

Centisome position: 54.78

GC content: 43.11

Gene sequence:

>1053_bases
ATGGATATGGCACAACATTTGTCTGAAACAGAGCTGTCAGCAAAAATACTGCGCCACGCTGTACCAAAAATGTCAGAATT
AAATATTCCTGTCACCCCTGAAAACTATGCGGTTTGGTATGAATACTATAAAGGGATTAATCTCGATCTGAAGCGTGCGA
TAGATGGTTTATTAGCTAATAACCTTGCCTTTACTCCTACCATGAGTGAGGAGCTCTATAGGACTTATATCCAGGTCCAC
TCACCTGAACTCATCGAAAATGTTCAAACAGAAACTCACATACTCATCAATAGCTTATTGGCAAAGATAGCGAGTATGAG
TAAGGGCACATCACGTTTTTCTGAGTCGTTGACAGAATTTGACACGGCTCTCAAAGATGATCCTGAACCTGCTGTATTAC
AGGATTTAGTGAACGGTATAACCTCTGAGCTCGATGAAATAATCAGTGCCAATATTGATATGGATAAGAGCCTCACCAGC
ATGAGTGAAGAGGTAGATGCGCTAAAAACAGAGATGGTCGAACTTCGTTCTGTAGTGATGACAGACCAACTGACGTCACT
GAATAATCGCCGAGCATTTGACAAAGAAGTGATAAGCCACATCGATAGTTTCAATCTTTCGAACGTTGAAAGTAGCTTGT
TGGTTGTCGATATAGATTACTTTAAGAAATTTAACGATGTTCACGGCCATTTAATCGGTGATAAGGTGCTCGCCTATGTG
GCTCAGGCACTTAAGAATGCCGTGAAAGGGGAAGATTTTGTGGCACGTTATGGCGGCGAGGAGTTTGTGGTCTTGCTGCC
TAATACCTCACATCAAGATGCCCATAAAGTTGCCGAGAATATTCGGATCAAGATAGCAGGTCGTAATCTAACCATAGGTA
AAGAGAAGAAGTTACCCCTTGGAAATGTCACTGTCTCGGTGGGCGTGGCCTCACTTAAGGCGAGCGACGATAAAGACAGT
TACTTTATTCGCGCCGATGAAGCCTTATACCGGGCAAAGTCATCTGGGCGTAACTGTGTTATTGGTGAAAGGGTTGCCGA
AGAACTGGTATAG

Upstream 100 bases:

>100_bases
AGTCATTAATCAGTAAATTTATTTGAAAAGGGGGTGGCAAGCCCCTTTTTTTTGTTTAAAGTATTGATAATCATGGAAAT
CGATCGAGAGGCGTCAATTT

Downstream 100 bases:

>100_bases
AACGAGCGCAGTCAGTTTACTCGTCATAAGCTCACTTGATTATGCACTTGAGCAAGCACTTAAATAAATAGAGCCATTAA
GAATCTGTCTTAATGGCTTT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDMAQHLSETELSAKILRHAVPKMSELNIPVTPENYAVWYEYYKGINLDLKRAIDGLLANNLAFTPTMSEELYRTYIQVH
SPELIENVQTETHILINSLLAKIASMSKGTSRFSESLTEFDTALKDDPEPAVLQDLVNGITSELDEIISANIDMDKSLTS
MSEEVDALKTEMVELRSVVMTDQLTSLNNRRAFDKEVISHIDSFNLSNVESSLLVVDIDYFKKFNDVHGHLIGDKVLAYV
AQALKNAVKGEDFVARYGGEEFVVLLPNTSHQDAHKVAENIRIKIAGRNLTIGKEKKLPLGNVTVSVGVASLKASDDKDS
YFIRADEALYRAKSSGRNCVIGERVAEELV

Sequences:

>Translated_350_residues
MDMAQHLSETELSAKILRHAVPKMSELNIPVTPENYAVWYEYYKGINLDLKRAIDGLLANNLAFTPTMSEELYRTYIQVH
SPELIENVQTETHILINSLLAKIASMSKGTSRFSESLTEFDTALKDDPEPAVLQDLVNGITSELDEIISANIDMDKSLTS
MSEEVDALKTEMVELRSVVMTDQLTSLNNRRAFDKEVISHIDSFNLSNVESSLLVVDIDYFKKFNDVHGHLIGDKVLAYV
AQALKNAVKGEDFVARYGGEEFVVLLPNTSHQDAHKVAENIRIKIAGRNLTIGKEKKLPLGNVTVSVGVASLKASDDKDS
YFIRADEALYRAKSSGRNCVIGERVAEELV
>Mature_350_residues
MDMAQHLSETELSAKILRHAVPKMSELNIPVTPENYAVWYEYYKGINLDLKRAIDGLLANNLAFTPTMSEELYRTYIQVH
SPELIENVQTETHILINSLLAKIASMSKGTSRFSESLTEFDTALKDDPEPAVLQDLVNGITSELDEIISANIDMDKSLTS
MSEEVDALKTEMVELRSVVMTDQLTSLNNRRAFDKEVISHIDSFNLSNVESSLLVVDIDYFKKFNDVHGHLIGDKVLAYV
AQALKNAVKGEDFVARYGGEEFVVLLPNTSHQDAHKVAENIRIKIAGRNLTIGKEKKLPLGNVTVSVGVASLKASDDKDS
YFIRADEALYRAKSSGRNCVIGERVAEELV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=192, Percent_Identity=36.4583333333333, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=36.3636363636364, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=35.8823529411765, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787802, Length=144, Percent_Identity=40.9722222222222, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=31.6455696202532, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787262, Length=198, Percent_Identity=32.3232323232323, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787816, Length=263, Percent_Identity=29.277566539924, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787541, Length=187, Percent_Identity=35.2941176470588, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=30.625, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=27.741935483871, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=30.188679245283, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788085, Length=197, Percent_Identity=28.4263959390863, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87081977, Length=175, Percent_Identity=29.7142857142857, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38968; Mature: 38968

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMAQHLSETELSAKILRHAVPKMSELNIPVTPENYAVWYEYYKGINLDLKRAIDGLLAN
CCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHC
NLAFTPTMSEELYRTYIQVHSPELIENVQTETHILINSLLAKIASMSKGTSRFSESLTEF
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DTALKDDPEPAVLQDLVNGITSELDEIISANIDMDKSLTSMSEEVDALKTEMVELRSVVM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TDQLTSLNNRRAFDKEVISHIDSFNLSNVESSLLVVDIDYFKKFNDVHGHLIGDKVLAYV
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHH
AQALKNAVKGEDFVARYGGEEFVVLLPNTSHQDAHKVAENIRIKIAGRNLTIGKEKKLPL
HHHHHHHHCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHCCEEEEECCEEECCCCCCCCC
GNVTVSVGVASLKASDDKDSYFIRADEALYRAKSSGRNCVIGERVAEELV
CCEEEEEEHHHHCCCCCCCCEEEEEHHHHHHHHCCCCCCEEHHHHHHHCC
>Mature Secondary Structure
MDMAQHLSETELSAKILRHAVPKMSELNIPVTPENYAVWYEYYKGINLDLKRAIDGLLAN
CCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHC
NLAFTPTMSEELYRTYIQVHSPELIENVQTETHILINSLLAKIASMSKGTSRFSESLTEF
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DTALKDDPEPAVLQDLVNGITSELDEIISANIDMDKSLTSMSEEVDALKTEMVELRSVVM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TDQLTSLNNRRAFDKEVISHIDSFNLSNVESSLLVVDIDYFKKFNDVHGHLIGDKVLAYV
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHH
AQALKNAVKGEDFVARYGGEEFVVLLPNTSHQDAHKVAENIRIKIAGRNLTIGKEKKLPL
HHHHHHHHCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHCCEEEEECCEEECCCCCCCCC
GNVTVSVGVASLKASDDKDSYFIRADEALYRAKSSGRNCVIGERVAEELV
CCEEEEEEHHHHCCCCCCCCEEEEEHHHHHHHHCCCCCCEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA