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The map label for this gene is pldA [C]

Identifier: 157962111

GI number: 157962111

Start: 2774152

End: 2775009

Strand: Reverse

Name: pldA [C]

Synonym: Spea_2290

Alternate gene names: 157962111

Gene position: 2775009-2774152 (Counterclockwise)

Preceding gene: 157962112

Following gene: 157962110

Centisome position: 53.63

GC content: 39.98

Gene sequence:

>858_bases
TTGAAGCATAAAACCAATATTAAAACCTATGTCATTTTACTAGCAAGCTTACTGGCTGCAGGTTCACTCCAGGCAAAAGA
GCAAGCCACTATTAGTAATGATATCACAGGGGTTGCAGGGTTAACTCCAGCTGATGATCTTTATAAGTTAACGGTGTTTG
ACAACAACTTCATTTTACCGATTTACCAAACGCAATCAGCAAATCAAGCTTACTACGCTCCCCAAAACCCAAATAATGAG
AACATTAGTAACACCAATCTGCAGTTTCAATTTAGTCTTAAATATGGCATCGCGAACAATCTCTTCACTGACAATGATGG
CTTTTACATCGCTTACAGCCAAATATCTAATTGGCAGGCTTACGACAAGTCGGCCTACTTTCGAGATTCACAATATCAGC
CGCAAGTGTTTTGGTTCTGGCAACACGGGAAGGCCGATGATACTTGGCGAAGTACTAGCCTAGGTTTCGAGCACCAATCA
AATGGCAAAGGTGGCATTTATGAGCGAAGTTGGAACCGCGTTTATTTGGAGTTTTCATTGGCATATAAGGATATTGAAAT
CAGTATAAAGCCTTGGCTAAGAGCTGACTTTTCAAGTACGGATTATAACCCTGATATTGAAGACTATATGGGCTATGGCA
CAGTCAAAGGTGATTGGTATTTTGGTGATCATCAAGTGAGCTTAACCCTAAGAAACCTTGTAGAAAGTGGTTTTTCTAAA
GGCTATGAAGAGTTGAGTTGGCGCTTTCCAATATATAAAGGCTTAAGAGGCTATCTAAAGCTGCAAAGTGGCTACGGGAT
GACCATTTCTGATTATAATCATTTTGATAATGCAGTAGGCATTGGTATTGCGCTTTAA

Upstream 100 bases:

>100_bases
AAATAAATCCAATATATTAGAATATTGAGACGAACATCTGGAAAATGATAAGTGCAATTGTTGATGAAGTTCGTCGTTAT
TTAATAGGGAGATAAGTAAC

Downstream 100 bases:

>100_bases
GATTAAATGATTATTGAATCACATTTACTATATTAGAACTTTCAAGGTTAAAAATGAACGAGTTTACCAGTTGGCTTGAT
TCTAAAAAGCTCAAACAGCA

Product: phospholipase A1

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKHKTNIKTYVILLASLLAAGSLQAKEQATISNDITGVAGLTPADDLYKLTVFDNNFILPIYQTQSANQAYYAPQNPNNE
NISNTNLQFQFSLKYGIANNLFTDNDGFYIAYSQISNWQAYDKSAYFRDSQYQPQVFWFWQHGKADDTWRSTSLGFEHQS
NGKGGIYERSWNRVYLEFSLAYKDIEISIKPWLRADFSSTDYNPDIEDYMGYGTVKGDWYFGDHQVSLTLRNLVESGFSK
GYEELSWRFPIYKGLRGYLKLQSGYGMTISDYNHFDNAVGIGIAL

Sequences:

>Translated_285_residues
MKHKTNIKTYVILLASLLAAGSLQAKEQATISNDITGVAGLTPADDLYKLTVFDNNFILPIYQTQSANQAYYAPQNPNNE
NISNTNLQFQFSLKYGIANNLFTDNDGFYIAYSQISNWQAYDKSAYFRDSQYQPQVFWFWQHGKADDTWRSTSLGFEHQS
NGKGGIYERSWNRVYLEFSLAYKDIEISIKPWLRADFSSTDYNPDIEDYMGYGTVKGDWYFGDHQVSLTLRNLVESGFSK
GYEELSWRFPIYKGLRGYLKLQSGYGMTISDYNHFDNAVGIGIAL
>Mature_285_residues
MKHKTNIKTYVILLASLLAAGSLQAKEQATISNDITGVAGLTPADDLYKLTVFDNNFILPIYQTQSANQAYYAPQNPNNE
NISNTNLQFQFSLKYGIANNLFTDNDGFYIAYSQISNWQAYDKSAYFRDSQYQPQVFWFWQHGKADDTWRSTSLGFEHQS
NGKGGIYERSWNRVYLEFSLAYKDIEISIKPWLRADFSSTDYNPDIEDYMGYGTVKGDWYFGDHQVSLTLRNLVESGFSK
GYEELSWRFPIYKGLRGYLKLQSGYGMTISDYNHFDNAVGIGIAL

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=289, Percent_Identity=31.1418685121107, Blast_Score=110, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 32667; Mature: 32667

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHKTNIKTYVILLASLLAAGSLQAKEQATISNDITGVAGLTPADDLYKLTVFDNNFILP
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCEEEE
IYQTQSANQAYYAPQNPNNENISNTNLQFQFSLKYGIANNLFTDNDGFYIAYSQISNWQA
EEECCCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCCEEEEEECCCCCCC
YDKSAYFRDSQYQPQVFWFWQHGKADDTWRSTSLGFEHQSNGKGGIYERSWNRVYLEFSL
CCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEEE
AYKDIEISIKPWLRADFSSTDYNPDIEDYMGYGTVKGDWYFGDHQVSLTLRNLVESGFSK
EEEEEEEEECCCEEECCCCCCCCCCHHHHCCCCEEECCEEECCEEEEEHHHHHHHHHHHC
GYEELSWRFPIYKGLRGYLKLQSGYGMTISDYNHFDNAVGIGIAL
CHHHHEEECCHHHCCEEEEEEECCCCCEECCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKHKTNIKTYVILLASLLAAGSLQAKEQATISNDITGVAGLTPADDLYKLTVFDNNFILP
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCEEEE
IYQTQSANQAYYAPQNPNNENISNTNLQFQFSLKYGIANNLFTDNDGFYIAYSQISNWQA
EEECCCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCCEEEEEECCCCCCC
YDKSAYFRDSQYQPQVFWFWQHGKADDTWRSTSLGFEHQSNGKGGIYERSWNRVYLEFSL
CCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEEE
AYKDIEISIKPWLRADFSSTDYNPDIEDYMGYGTVKGDWYFGDHQVSLTLRNLVESGFSK
EEEEEEEEECCCEEECCCCCCCCCCHHHHCCCCEEECCEEECCEEEEEHHHHHHHHHHHC
GYEELSWRFPIYKGLRGYLKLQSGYGMTISDYNHFDNAVGIGIAL
CHHHHEEECCHHHCCEEEEEEECCCCCEECCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]