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The map label for this gene is kshB [H]

Identifier: 157961951

GI number: 157961951

Start: 2581476

End: 2582561

Strand: Direct

Name: kshB [H]

Synonym: Spea_2130

Alternate gene names: 157961951

Gene position: 2581476-2582561 (Clockwise)

Preceding gene: 157961950

Following gene: 157961952

Centisome position: 49.89

GC content: 44.2

Gene sequence:

>1086_bases
ATGTCTTCATCTCGCTATCATCTGTTGACAGTCAGTAAACTGGTAAATGAAACCTCAGATGCACGTTCAGTGATTTTTGA
AGTGCCGCCGAGTTTACGTCAAAAATTTGCATTTAAAGCCGGGCAATTTCTTACATTCAGGATCCCTAAAAACGGCGAAC
ATCTACTGCGTTGTTATTCGTTGGCTAGTACACCAGACGAACCTATGTTGAAAGTTACCGTGAAACGGGTGGCTAATGGC
GTGGTATCAACTTGGGCGGTAAACGACTTGAAGGTCGGCGATCAAGTCGAGGTCATGCAACCTGCAGGTATTTTTATACC
TAAATCCGTAAGTGGTGACTTATTGTTGTGTGCTGGTGGAAGTGGCATCACCCCGATATTTTCAATTTTACAAACTGCCT
TGAAGCAAGGCTCAGGTAATATCCGCTTGATCTACGCCAATCGTGATCAAGACTCGGTCATTTTTAAAAATGAATTGATA
ACCCTAACAGAACAATATCCAAAACGGCTCGAGGTTATCCACCTTCTTGACTCCGTTTCAGGGATCCCAAGTAATCATTT
ACTGGCGCGATTAGCCCAACCCTTTGCTAGCACGCTTAACTTTGAGGGGGCATTTATTTGCGGTCCAGCACCTTTTATGG
ACTCAATGGAGTACGCATTAGAATCAATCAACTTGTCAGCCGACAAAATCTACGTAGAACGTTTTATTTCATTACCCAAT
GAAAAAATAGCGGGAGGGCAAGCAACTGACGTACCAAATAACCGAATCGAAACCGTTACACAACATAGTAATGGTGCTAG
CGATACGTTGATCGATGCTGTCGCCACGATCGAGCTCGATGGTCAAACCCATAATATAGACTGGAGCAAACAAGATACCC
TGCTTGAAGCCGCTGAAAAGGCTGGCCTATCTTTGCCCCATTCATGTAGAGAGGGAATGTGTGCATCTTGCATGTGCGAA
GTCAAAGAGGGCCAGGTTCAACTACGCGCAAATGAGGTTCTTAGCGAACGAGATTTAAAACAATCGCTAACCTTAAGCTG
TCAGGCAATGCCCCATTCTTCTAAAGTCCACATTTGCTATACGTAA

Upstream 100 bases:

>100_bases
CCTATATGCGCGCCCACGGTTCAGTCATGCTTGGGTTACCAAATCTTGATCCATTTATTTAATCATCTCATTAGATAACC
GTTTTTGGAAAGGAGTTGCC

Downstream 100 bases:

>100_bases
ACTTTCGACTATATGAATTCATGAATGAATCGAGTTCATGAATTCATATAGTCCCTATGCAGATGTTGGCTATTTATCTC
CTTAACCTTAGGGTTCGACA

Product: ferredoxin

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSSSRYHLLTVSKLVNETSDARSVIFEVPPSLRQKFAFKAGQFLTFRIPKNGEHLLRCYSLASTPDEPMLKVTVKRVANG
VVSTWAVNDLKVGDQVEVMQPAGIFIPKSVSGDLLLCAGGSGITPIFSILQTALKQGSGNIRLIYANRDQDSVIFKNELI
TLTEQYPKRLEVIHLLDSVSGIPSNHLLARLAQPFASTLNFEGAFICGPAPFMDSMEYALESINLSADKIYVERFISLPN
EKIAGGQATDVPNNRIETVTQHSNGASDTLIDAVATIELDGQTHNIDWSKQDTLLEAAEKAGLSLPHSCREGMCASCMCE
VKEGQVQLRANEVLSERDLKQSLTLSCQAMPHSSKVHICYT

Sequences:

>Translated_361_residues
MSSSRYHLLTVSKLVNETSDARSVIFEVPPSLRQKFAFKAGQFLTFRIPKNGEHLLRCYSLASTPDEPMLKVTVKRVANG
VVSTWAVNDLKVGDQVEVMQPAGIFIPKSVSGDLLLCAGGSGITPIFSILQTALKQGSGNIRLIYANRDQDSVIFKNELI
TLTEQYPKRLEVIHLLDSVSGIPSNHLLARLAQPFASTLNFEGAFICGPAPFMDSMEYALESINLSADKIYVERFISLPN
EKIAGGQATDVPNNRIETVTQHSNGASDTLIDAVATIELDGQTHNIDWSKQDTLLEAAEKAGLSLPHSCREGMCASCMCE
VKEGQVQLRANEVLSERDLKQSLTLSCQAMPHSSKVHICYT
>Mature_360_residues
SSSRYHLLTVSKLVNETSDARSVIFEVPPSLRQKFAFKAGQFLTFRIPKNGEHLLRCYSLASTPDEPMLKVTVKRVANGV
VSTWAVNDLKVGDQVEVMQPAGIFIPKSVSGDLLLCAGGSGITPIFSILQTALKQGSGNIRLIYANRDQDSVIFKNELIT
LTEQYPKRLEVIHLLDSVSGIPSNHLLARLAQPFASTLNFEGAFICGPAPFMDSMEYALESINLSADKIYVERFISLPNE
KIAGGQATDVPNNRIETVTQHSNGASDTLIDAVATIELDGQTHNIDWSKQDTLLEAAEKAGLSLPHSCREGMCASCMCEV
KEGQVQLRANEVLSERDLKQSLTLSCQAMPHSSKVHICYT

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI19923907, Length=267, Percent_Identity=25.8426966292135, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787658, Length=365, Percent_Identity=34.2465753424658, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787098, Length=353, Percent_Identity=24.0793201133144, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788903, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788104, Length=316, Percent_Identity=25.3164556962025, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323552, Length=198, Percent_Identity=29.7979797979798, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323510, Length=133, Percent_Identity=33.0827067669173, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39513; Mature: 39381

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSRYHLLTVSKLVNETSDARSVIFEVPPSLRQKFAFKAGQFLTFRIPKNGEHLLRCYS
CCCCCEEEEEHHHHHCCCCCHHHEEEECCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LASTPDEPMLKVTVKRVANGVVSTWAVNDLKVGDQVEVMQPAGIFIPKSVSGDLLLCAGG
HCCCCCCCHHHHHHHHHHCCHHHHEEECCCCCCCCEEEECCCCEEEECCCCCCEEEEECC
SGITPIFSILQTALKQGSGNIRLIYANRDQDSVIFKNELITLTEQYPKRLEVIHLLDSVS
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHC
GIPSNHLLARLAQPFASTLNFEGAFICGPAPFMDSMEYALESINLSADKIYVERFISLPN
CCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
EKIAGGQATDVPNNRIETVTQHSNGASDTLIDAVATIELDGQTHNIDWSKQDTLLEAAEK
CCCCCCCCCCCCCCHHHEEEECCCCCCHHHEEEEEEEEECCCCCCCCCCHHHHHHHHHHH
AGLSLPHSCREGMCASCMCEVKEGQVQLRANEVLSERDLKQSLTLSCQAMPHSSKVHICY
CCCCCCHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCEEEEECCCCCEEEEEE
T
C
>Mature Secondary Structure 
SSSRYHLLTVSKLVNETSDARSVIFEVPPSLRQKFAFKAGQFLTFRIPKNGEHLLRCYS
CCCCEEEEEHHHHHCCCCCHHHEEEECCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LASTPDEPMLKVTVKRVANGVVSTWAVNDLKVGDQVEVMQPAGIFIPKSVSGDLLLCAGG
HCCCCCCCHHHHHHHHHHCCHHHHEEECCCCCCCCEEEECCCCEEEECCCCCCEEEEECC
SGITPIFSILQTALKQGSGNIRLIYANRDQDSVIFKNELITLTEQYPKRLEVIHLLDSVS
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHC
GIPSNHLLARLAQPFASTLNFEGAFICGPAPFMDSMEYALESINLSADKIYVERFISLPN
CCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
EKIAGGQATDVPNNRIETVTQHSNGASDTLIDAVATIELDGQTHNIDWSKQDTLLEAAEK
CCCCCCCCCCCCCCHHHEEEECCCCCCHHHEEEEEEEEECCCCCCCCCCHHHHHHHHHHH
AGLSLPHSCREGMCASCMCEVKEGQVQLRANEVLSERDLKQSLTLSCQAMPHSSKVHICY
CCCCCCHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCEEEEECCCCCEEEEEE
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA