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The map label for this gene is caiC [H]

Identifier: 157961945

GI number: 157961945

Start: 2570076

End: 2571773

Strand: Direct

Name: caiC [H]

Synonym: Spea_2124

Alternate gene names: 157961945

Gene position: 2570076-2571773 (Clockwise)

Preceding gene: 157961944

Following gene: 157961946

Centisome position: 49.67

GC content: 45.64

Gene sequence:

>1698_bases
ATGGATATCATTGATCAAGCACAAAAAGTTAAAGCGCAACTGACAGCAGCAGGGGCGCCATTTGAATTGACGCAACTTGA
TCAATCAGGGGGCGTTGTGTACCGTAACGCGCCTAACAATCTCGCCGAAGTCATAGAGTCATCACGTCGTGATGATGAAA
GTACTTTCCTAGTTTATCAAGATGATGTTTTCAGTTTCAGTCGATTCTACACTGAAGTCGACGCGCTTGCCGCGTGGTTC
CATAAGCAGGGAATCGTACAAGGCTCTCGCGTCGCGATAGCATTACGCAATCGCCCCGAATGGGCGGTAACATTTGCAGC
TATTGCAAAGTTGGGCGCCGTTCCGGTTCCGCTCAACAGTTTTGGACAGCCGAAAGAACTGTGGTCGGCGATAGACGAGG
TGATGCCCAAGATTTTAGTGTGCGATAAAGCGCGCTGGAATAAACTGGATACCCATGTAGACAGTAATACTTTTGATGTG
TTAGTTGTAGATGACAACGATAAGGCGACGCCGGATGTATCACATTACCAAGAGGTGGTCGCTAGCTACAAGCATTCAGA
AACCGTTTTTCCTCATCTAAGTATCTTACCAGAAGATACCGCCCTGATTTTGTTTACCTCAGGCGCTTCAAGCAATGCAA
AAGCGGTCGTCTCCTCTCAACGAGCTGTTTGTCAGGCGCTTTTCAATATCGATTTTATCTCTGCCATATCGGCAATGACA
TCACCAAAAGTAGTTGAAAGGATAATGGCTAAAGGCAATCCTCCAATGTTACTTACTGCCGTCCCGTTATTTCATGTCAG
TGGCCTGCACGCTCAGTTATTAACCGCGCTACGAAGTGGTAGAGGAATGGTGATAATGCATCGCTGGGATCCTAAAGAAG
CGGTGAAAATGCTCAAGCAGCATAATATCACTCAGTTTAATGGTGCGCCTTCTATGGTGATGCAGCTATTTCGTGAGCCA
GGATTTCTGTCACCGACAGTATTAGGCAATATGGCAGGCCTAGGCTTTGGTGGCTCAGGCTTACCAGAAGTGCTAGTCGA
TCTAGTATTAAAAGAAATGCCTAATCATATGGTTGGTAGTGGTTTCGGCATGACAGAATCTAACGGTGTGGGCGCGTCTT
CGTCTGGCGAAATGTTCAGAGTCAGCCCTAAAAGTTCGGGAATGTTATCCCCTTTAGTCAAGGTAAAAGTCTGTGATCCG
ATAGGGGATGAACTGCCAAAGGGAGAAGTTGGAGAGATTTGCCTCAAGTCCGTCACGATTATGCGTGAATACCTGAATAA
TAACCAAGGTACTCAACAAGCTATTCAGAATGGTTGGCTGCACACCGGTGATATTGGCTATTTAGATCGCCATGGTTTCT
TGTTCGTAGTGGATAGGCTTAAAAATGTAATCATCAGAAATGGTGAGAATATTGCATCGGTCGAAGTCGAATCCAGCTTA
ATGTTACACCATGCTATTAAAGAGGCTGCAGTATTTGGTATTGCCGATGACATGGTTGGTGAAAGCGTTATGGCCGTCGT
GTCGCTAAAAAGAGGACAACAGGTTGAGGAAGATGAGCTGAAGCTGCATGTTGCCGCTCAACTTGCAGGCTATAAAGTAC
CAAGTACCATTCATATTGTCGAAGATGACTTACCACGGAATCCAGCGGGTAAGTTGCTGCATAGCCAATTGAAACAAACT
TATGGATTACGTAAATAA

Upstream 100 bases:

>100_bases
AAAGACCCTTTGTAATAAAAGTAATAAATATTTATATAACAATAAGCTAAGAATAGCTTAGGGATTATAGTTAACAAGAA
ATAGATAACAGGAGAAAGTG

Downstream 100 bases:

>100_bases
GGTTATGTTATTGCCAACGCCACAACCCCGTTTGGCGTTGGCATCCTAGATTAGGTCTTTGTTTATTATCTATAATTAGT
AATGCTGTAAAGCCAGTTTG

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MDIIDQAQKVKAQLTAAGAPFELTQLDQSGGVVYRNAPNNLAEVIESSRRDDESTFLVYQDDVFSFSRFYTEVDALAAWF
HKQGIVQGSRVAIALRNRPEWAVTFAAIAKLGAVPVPLNSFGQPKELWSAIDEVMPKILVCDKARWNKLDTHVDSNTFDV
LVVDDNDKATPDVSHYQEVVASYKHSETVFPHLSILPEDTALILFTSGASSNAKAVVSSQRAVCQALFNIDFISAISAMT
SPKVVERIMAKGNPPMLLTAVPLFHVSGLHAQLLTALRSGRGMVIMHRWDPKEAVKMLKQHNITQFNGAPSMVMQLFREP
GFLSPTVLGNMAGLGFGGSGLPEVLVDLVLKEMPNHMVGSGFGMTESNGVGASSSGEMFRVSPKSSGMLSPLVKVKVCDP
IGDELPKGEVGEICLKSVTIMREYLNNNQGTQQAIQNGWLHTGDIGYLDRHGFLFVVDRLKNVIIRNGENIASVEVESSL
MLHHAIKEAAVFGIADDMVGESVMAVVSLKRGQQVEEDELKLHVAAQLAGYKVPSTIHIVEDDLPRNPAGKLLHSQLKQT
YGLRK

Sequences:

>Translated_565_residues
MDIIDQAQKVKAQLTAAGAPFELTQLDQSGGVVYRNAPNNLAEVIESSRRDDESTFLVYQDDVFSFSRFYTEVDALAAWF
HKQGIVQGSRVAIALRNRPEWAVTFAAIAKLGAVPVPLNSFGQPKELWSAIDEVMPKILVCDKARWNKLDTHVDSNTFDV
LVVDDNDKATPDVSHYQEVVASYKHSETVFPHLSILPEDTALILFTSGASSNAKAVVSSQRAVCQALFNIDFISAISAMT
SPKVVERIMAKGNPPMLLTAVPLFHVSGLHAQLLTALRSGRGMVIMHRWDPKEAVKMLKQHNITQFNGAPSMVMQLFREP
GFLSPTVLGNMAGLGFGGSGLPEVLVDLVLKEMPNHMVGSGFGMTESNGVGASSSGEMFRVSPKSSGMLSPLVKVKVCDP
IGDELPKGEVGEICLKSVTIMREYLNNNQGTQQAIQNGWLHTGDIGYLDRHGFLFVVDRLKNVIIRNGENIASVEVESSL
MLHHAIKEAAVFGIADDMVGESVMAVVSLKRGQQVEEDELKLHVAAQLAGYKVPSTIHIVEDDLPRNPAGKLLHSQLKQT
YGLRK
>Mature_565_residues
MDIIDQAQKVKAQLTAAGAPFELTQLDQSGGVVYRNAPNNLAEVIESSRRDDESTFLVYQDDVFSFSRFYTEVDALAAWF
HKQGIVQGSRVAIALRNRPEWAVTFAAIAKLGAVPVPLNSFGQPKELWSAIDEVMPKILVCDKARWNKLDTHVDSNTFDV
LVVDDNDKATPDVSHYQEVVASYKHSETVFPHLSILPEDTALILFTSGASSNAKAVVSSQRAVCQALFNIDFISAISAMT
SPKVVERIMAKGNPPMLLTAVPLFHVSGLHAQLLTALRSGRGMVIMHRWDPKEAVKMLKQHNITQFNGAPSMVMQLFREP
GFLSPTVLGNMAGLGFGGSGLPEVLVDLVLKEMPNHMVGSGFGMTESNGVGASSSGEMFRVSPKSSGMLSPLVKVKVCDP
IGDELPKGEVGEICLKSVTIMREYLNNNQGTQQAIQNGWLHTGDIGYLDRHGFLFVVDRLKNVIIRNGENIASVEVESSL
MLHHAIKEAAVFGIADDMVGESVMAVVSLKRGQQVEEDELKLHVAAQLAGYKVPSTIHIVEDDLPRNPAGKLLHSQLKQT
YGLRK

Specific function: Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=537, Percent_Identity=24.5810055865922, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI187761345, Length=389, Percent_Identity=27.2493573264781, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI187761343, Length=389, Percent_Identity=27.2493573264781, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI38505220, Length=503, Percent_Identity=24.4532803180915, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI42544132, Length=495, Percent_Identity=23.6363636363636, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI122937307, Length=208, Percent_Identity=33.1730769230769, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI115511026, Length=495, Percent_Identity=23.2323232323232, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI157311624, Length=493, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI157311622, Length=493, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI58082049, Length=499, Percent_Identity=23.6472945891784, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI27477105, Length=490, Percent_Identity=23.0612244897959, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI28416953, Length=574, Percent_Identity=22.4738675958188, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI13325057, Length=387, Percent_Identity=25.3229974160207, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI221142682, Length=511, Percent_Identity=28.3757338551859, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788107, Length=549, Percent_Identity=26.2295081967213, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI145693145, Length=530, Percent_Identity=25.0943396226415, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1786810, Length=526, Percent_Identity=25.2851711026616, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788595, Length=486, Percent_Identity=22.6337448559671, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790505, Length=609, Percent_Identity=21.0180623973727, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71994690, Length=547, Percent_Identity=24.8628884826325, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=547, Percent_Identity=24.8628884826325, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=547, Percent_Identity=24.8628884826325, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17557194, Length=500, Percent_Identity=25.6, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=519, Percent_Identity=24.4701348747592, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17558820, Length=522, Percent_Identity=26.6283524904215, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=307, Percent_Identity=31.5960912052117, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=307, Percent_Identity=30.9446254071661, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=526, Percent_Identity=22.0532319391635, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=535, Percent_Identity=25.981308411215, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=498, Percent_Identity=23.8955823293173, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=516, Percent_Identity=25, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17510401, Length=478, Percent_Identity=23.2217573221757, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25141359, Length=530, Percent_Identity=23.0188679245283, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=376, Percent_Identity=25.2659574468085, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=502, Percent_Identity=26.0956175298805, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6323182, Length=588, Percent_Identity=24.3197278911565, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=529, Percent_Identity=20.7939508506616, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24581924, Length=557, Percent_Identity=27.6481149012567, Blast_Score=172, Evalue=7e-43,
Organism=Drosophila melanogaster, GI21355181, Length=505, Percent_Identity=26.5346534653465, Blast_Score=165, Evalue=7e-41,
Organism=Drosophila melanogaster, GI18859661, Length=516, Percent_Identity=25, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24648260, Length=428, Percent_Identity=24.7663551401869, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI281365686, Length=333, Percent_Identity=27.027027027027, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21356441, Length=288, Percent_Identity=26.7361111111111, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356947, Length=531, Percent_Identity=22.5988700564972, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653035, Length=503, Percent_Identity=22.2664015904573, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161076582, Length=517, Percent_Identity=22.6305609284333, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI20130357, Length=531, Percent_Identity=21.4689265536723, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648253, Length=485, Percent_Identity=23.0927835051546, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648255, Length=485, Percent_Identity=23.0927835051546, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21358303, Length=263, Percent_Identity=25.8555133079848, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922652, Length=500, Percent_Identity=23.2, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=22.0883534136546, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648257, Length=398, Percent_Identity=25.1256281407035, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 61666; Mature: 61666

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIDQAQKVKAQLTAAGAPFELTQLDQSGGVVYRNAPNNLAEVIESSRRDDESTFLVYQ
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEE
DDVFSFSRFYTEVDALAAWFHKQGIVQGSRVAIALRNRPEWAVTFAAIAKLGAVPVPLNS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCC
FGQPKELWSAIDEVMPKILVCDKARWNKLDTHVDSNTFDVLVVDDNDKATPDVSHYQEVV
CCCHHHHHHHHHHHHHHHEEECCCCCCCHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHH
ASYKHSETVFPHLSILPEDTALILFTSGASSNAKAVVSSQRAVCQALFNIDFISAISAMT
HHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPKVVERIMAKGNPPMLLTAVPLFHVSGLHAQLLTALRSGRGMVIMHRWDPKEAVKMLKQ
CHHHHHHHHHCCCCCEEEEEEHHHHHCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
HNITQFNGAPSMVMQLFREPGFLSPTVLGNMAGLGFGGSGLPEVLVDLVLKEMPNHMVGS
CCCCEECCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCC
GFGMTESNGVGASSSGEMFRVSPKSSGMLSPLVKVKVCDPIGDELPKGEVGEICLKSVTI
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
MREYLNNNQGTQQAIQNGWLHTGDIGYLDRHGFLFVVDRLKNVIIRNGENIASVEVESSL
HHHHHCCCCCHHHHHHCCCEECCCCCEECCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHH
MLHHAIKEAAVFGIADDMVGESVMAVVSLKRGQQVEEDELKLHVAAQLAGYKVPSTIHIV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEHHHHCCCCCCEEEEE
EDDLPRNPAGKLLHSQLKQTYGLRK
CCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDIIDQAQKVKAQLTAAGAPFELTQLDQSGGVVYRNAPNNLAEVIESSRRDDESTFLVYQ
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEE
DDVFSFSRFYTEVDALAAWFHKQGIVQGSRVAIALRNRPEWAVTFAAIAKLGAVPVPLNS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCC
FGQPKELWSAIDEVMPKILVCDKARWNKLDTHVDSNTFDVLVVDDNDKATPDVSHYQEVV
CCCHHHHHHHHHHHHHHHEEECCCCCCCHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHH
ASYKHSETVFPHLSILPEDTALILFTSGASSNAKAVVSSQRAVCQALFNIDFISAISAMT
HHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPKVVERIMAKGNPPMLLTAVPLFHVSGLHAQLLTALRSGRGMVIMHRWDPKEAVKMLKQ
CHHHHHHHHHCCCCCEEEEEEHHHHHCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
HNITQFNGAPSMVMQLFREPGFLSPTVLGNMAGLGFGGSGLPEVLVDLVLKEMPNHMVGS
CCCCEECCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCC
GFGMTESNGVGASSSGEMFRVSPKSSGMLSPLVKVKVCDPIGDELPKGEVGEICLKSVTI
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
MREYLNNNQGTQQAIQNGWLHTGDIGYLDRHGFLFVVDRLKNVIIRNGENIASVEVESSL
HHHHHCCCCCHHHHHHCCCEECCCCCEECCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHH
MLHHAIKEAAVFGIADDMVGESVMAVVSLKRGQQVEEDELKLHVAAQLAGYKVPSTIHIV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEHHHHCCCCCCEEEEE
EDDLPRNPAGKLLHSQLKQTYGLRK
CCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA