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The map label for this gene is kshA [H]

Identifier: 157961942

GI number: 157961942

Start: 2566171

End: 2567304

Strand: Reverse

Name: kshA [H]

Synonym: Spea_2121

Alternate gene names: 157961942

Gene position: 2567304-2566171 (Counterclockwise)

Preceding gene: 157961943

Following gene: 157961940

Centisome position: 49.61

GC content: 44.36

Gene sequence:

>1134_bases
ATGAATAATATAAAAAACATAATTAGCAAGGAGTTACGCATGGCAACAGCACAAGATTACCGTTTAGGTGATTTCGACTT
CCCTAGAGGCTGGTTTATGATCGCCACCGCCGAGGATATTAATACTCACAAGCCTTTAGCCGTTAGGTTTTTTGGACGCG
ACTTTGCACTATACCGTGGCCAAGCCACTGGTAGAGTTGTATTACTCGATGCGTACTGCCCTCATATGAAGACGCATCTG
GCCGCTGAAAATAATACTTCTTATGTCATCATCGACGGTGGTGGAACTAATATCGATGGTGACGGAATTCGTTGTCCTTA
TCATGCATGGCGTTTTGGTCCAGATGGGCAATGTGATGATATTCCTTACCATGACGGTCCAATTCCTAAAACTGCTTGTG
TAAAATCCTGGCCGGTAACAGAAAGCCTAGGTGCAATTTGGGTTTGGCATGATCCCGAAAATGGCGATCCAGAGTGGGAT
CATCCATCGCTTCCCCAATGGGATGACGATTCTTGGGTTAAATGGCAGTTCGATGACTTAGGGATCCTTGAACAACATCC
GCAAGAAGTGATCGACAACATTTGTGATTATGGCCACTTGAGCCCGATCCACGGTTCGACAGTACAACGCTATGAAAATG
AATTTAAAGGCCATCAAGCTATTCAAAGACAATGTGGACCTCATCGAACTCTAGTTAGTGAAGATGGCGGTAGCCCAATA
TTGCATACTGATACTAGTTATCACGGCCCTGGAGTACTCATATCCCATATGACGGGGTTTTATGACGCTATTTTGATGAT
AACAAATACGCCAGTAGAAGATGGTTCTGTCCGAGTCTGGCATGCGTTACTCGTTAAGTCACCAAATGGCAACGGTGTTG
TTACTGTTGAAGACACCATCGCTGCACGCCAATTTCAGCAAGCGAGTCGCCTCGCCTTTGAACAAGATTTTGAGGTTTGG
ACTAATAAAGCCCCTTGTCTAAATGGACTCTTTATCCCCAGTGATGGACCGTTTATGAAAGTGCGTATTTGGTATAAGCA
ATTTTATAACCCTAAAGCACGACGAGCTGAATTTTTAGATAAATGCGAGGGCGTTTACGTACCTAAGGGCACTGAACCTT
ATACAATCCCTTAG

Upstream 100 bases:

>100_bases
GCCACCGACTACCGTTAACGATCCACTTCCAAGTACATAGATAATTAAAACGATAATTAAAACGATAATTATACCGGCAC
GGTTAGGTAATAGTGCCGGA

Downstream 100 bases:

>100_bases
ATTGTTATTAATAAAAACATAAAAATGGCAGCCTGTGGCTGCCATTTTTCATTATTGCTAACAATTAATCCATTTCGTTA
ATCTGCTGTAACTTGCGACT

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNNIKNIISKELRMATAQDYRLGDFDFPRGWFMIATAEDINTHKPLAVRFFGRDFALYRGQATGRVVLLDAYCPHMKTHL
AAENNTSYVIIDGGGTNIDGDGIRCPYHAWRFGPDGQCDDIPYHDGPIPKTACVKSWPVTESLGAIWVWHDPENGDPEWD
HPSLPQWDDDSWVKWQFDDLGILEQHPQEVIDNICDYGHLSPIHGSTVQRYENEFKGHQAIQRQCGPHRTLVSEDGGSPI
LHTDTSYHGPGVLISHMTGFYDAILMITNTPVEDGSVRVWHALLVKSPNGNGVVTVEDTIAARQFQQASRLAFEQDFEVW
TNKAPCLNGLFIPSDGPFMKVRIWYKQFYNPKARRAEFLDKCEGVYVPKGTEPYTIP

Sequences:

>Translated_377_residues
MNNIKNIISKELRMATAQDYRLGDFDFPRGWFMIATAEDINTHKPLAVRFFGRDFALYRGQATGRVVLLDAYCPHMKTHL
AAENNTSYVIIDGGGTNIDGDGIRCPYHAWRFGPDGQCDDIPYHDGPIPKTACVKSWPVTESLGAIWVWHDPENGDPEWD
HPSLPQWDDDSWVKWQFDDLGILEQHPQEVIDNICDYGHLSPIHGSTVQRYENEFKGHQAIQRQCGPHRTLVSEDGGSPI
LHTDTSYHGPGVLISHMTGFYDAILMITNTPVEDGSVRVWHALLVKSPNGNGVVTVEDTIAARQFQQASRLAFEQDFEVW
TNKAPCLNGLFIPSDGPFMKVRIWYKQFYNPKARRAEFLDKCEGVYVPKGTEPYTIP
>Mature_377_residues
MNNIKNIISKELRMATAQDYRLGDFDFPRGWFMIATAEDINTHKPLAVRFFGRDFALYRGQATGRVVLLDAYCPHMKTHL
AAENNTSYVIIDGGGTNIDGDGIRCPYHAWRFGPDGQCDDIPYHDGPIPKTACVKSWPVTESLGAIWVWHDPENGDPEWD
HPSLPQWDDDSWVKWQFDDLGILEQHPQEVIDNICDYGHLSPIHGSTVQRYENEFKGHQAIQRQCGPHRTLVSEDGGSPI
LHTDTSYHGPGVLISHMTGFYDAILMITNTPVEDGSVRVWHALLVKSPNGNGVVTVEDTIAARQFQQASRLAFEQDFEVW
TNKAPCLNGLFIPSDGPFMKVRIWYKQFYNPKARRAEFLDKCEGVYVPKGTEPYTIP

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta- cleavage [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI71983709, Length=358, Percent_Identity=26.2569832402235, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI161085896, Length=342, Percent_Identity=25.7309941520468, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: NA

Molecular weight: Translated: 42676; Mature: 42676

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIKNIISKELRMATAQDYRLGDFDFPRGWFMIATAEDINTHKPLAVRFFGRDFALYRG
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCEEEEEEC
QATGRVVLLDAYCPHMKTHLAAENNTSYVIIDGGGTNIDGDGIRCPYHAWRFGPDGQCDD
CCCCEEEEEECCCCCHHHHEEECCCCCEEEEECCCCCCCCCCEECCHHHEECCCCCCCCC
IPYHDGPIPKTACVKSWPVTESLGAIWVWHDPENGDPEWDHPSLPQWDDDSWVKWQFDDL
CCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCC
GILEQHPQEVIDNICDYGHLSPIHGSTVQRYENEFKGHQAIQRQCGPHRTLVSEDGGSPI
CHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCE
LHTDTSYHGPGVLISHMTGFYDAILMITNTPVEDGSVRVWHALLVKSPNGNGVVTVEDTI
EECCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCEEEEEEEEEECCCCCEEEEECHHH
AARQFQQASRLAFEQDFEVWTNKAPCLNGLFIPSDGPFMKVRIWYKQFYNPKARRAEFLD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEEEHHHCCCHHHHHHHHH
KCEGVYVPKGTEPYTIP
HHCCEECCCCCCCCCCC
>Mature Secondary Structure
MNNIKNIISKELRMATAQDYRLGDFDFPRGWFMIATAEDINTHKPLAVRFFGRDFALYRG
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCEEEEEEC
QATGRVVLLDAYCPHMKTHLAAENNTSYVIIDGGGTNIDGDGIRCPYHAWRFGPDGQCDD
CCCCEEEEEECCCCCHHHHEEECCCCCEEEEECCCCCCCCCCEECCHHHEECCCCCCCCC
IPYHDGPIPKTACVKSWPVTESLGAIWVWHDPENGDPEWDHPSLPQWDDDSWVKWQFDDL
CCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCC
GILEQHPQEVIDNICDYGHLSPIHGSTVQRYENEFKGHQAIQRQCGPHRTLVSEDGGSPI
CHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCE
LHTDTSYHGPGVLISHMTGFYDAILMITNTPVEDGSVRVWHALLVKSPNGNGVVTVEDTI
EECCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCEEEEEEEEEECCCCCEEEEECHHH
AARQFQQASRLAFEQDFEVWTNKAPCLNGLFIPSDGPFMKVRIWYKQFYNPKARRAEFLD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEEEHHHCCCHHHHHHHHH
KCEGVYVPKGTEPYTIP
HHCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA