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The map label for this gene is fixJ [H]

Identifier: 157961909

GI number: 157961909

Start: 2527340

End: 2527939

Strand: Reverse

Name: fixJ [H]

Synonym: Spea_2088

Alternate gene names: 157961909

Gene position: 2527939-2527340 (Counterclockwise)

Preceding gene: 157961910

Following gene: 157961904

Centisome position: 48.85

GC content: 45.0

Gene sequence:

>600_bases
TTGTGTAACTTATATCTAGTCGACGACGATCAAGCGGTATTAGACTCTTTGACTTGGATGTTAAATGGGCTTGGCTTCCA
ACCCCAAGGATTCCTGTCCGCCGACAGTTTCTTAAATCAAGTCAATCTTCACAATGAAGGCATTGCAGTCTTAGATGTAC
AGATGCCAGGTATGGATGGTAGCGCGCTACTGTCACTCCTCACTAAGGCTCAAAGTCCTATCGCTGTGATCATGTTGAGT
GGCCATGGCAATATTGCTATGGCGGTACAAGCGATTCAAAGAGGGGCTTTAGACTTTCTTGAAAAGCCCGTAGATGGTGA
CAAGCTTGTCGTGCTACTTGAGCAGGCCAAAACCCAAACAAAGCTTAACATGCAGCGTAAACTAGCACGTGAAGCGCTAA
GCGATAAGCTTGAAGCACTGACTCCTAGAGAGCATGAAGTGATGGAAAAGGTACTAGAGGGTAAGCTCAATAAAGTGATT
GCTGCCGAGCTTAATATTGCACAGCGAACTCTAGAATTACACCGTCAGAAAGTGATGCAAAAAATGCAGGTCAGTAACGT
TGCCGAATTGGCTTTTTTGATGGGTAAGAGTACCGAATAG

Upstream 100 bases:

>100_bases
GAACATGACGGCGAGATCATCGCAGCTAACAATAACCTTGAAGGCTCGGCCGCTCCAGGTTTGACCATAACAATAACCCT
ACCTATTAAGGGGCCTCAAA

Downstream 100 bases:

>100_bases
TCGGTTCACTCCTTAATAAGTCACTTATGCGATACGACTACTTGGGCTGCGCCTAAATTGAGCCCAGCGGGCGCAGAACT
CCCAACCTTTGTCTTGGCAA

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MCNLYLVDDDQAVLDSLTWMLNGLGFQPQGFLSADSFLNQVNLHNEGIAVLDVQMPGMDGSALLSLLTKAQSPIAVIMLS
GHGNIAMAVQAIQRGALDFLEKPVDGDKLVVLLEQAKTQTKLNMQRKLAREALSDKLEALTPREHEVMEKVLEGKLNKVI
AAELNIAQRTLELHRQKVMQKMQVSNVAELAFLMGKSTE

Sequences:

>Translated_199_residues
MCNLYLVDDDQAVLDSLTWMLNGLGFQPQGFLSADSFLNQVNLHNEGIAVLDVQMPGMDGSALLSLLTKAQSPIAVIMLS
GHGNIAMAVQAIQRGALDFLEKPVDGDKLVVLLEQAKTQTKLNMQRKLAREALSDKLEALTPREHEVMEKVLEGKLNKVI
AAELNIAQRTLELHRQKVMQKMQVSNVAELAFLMGKSTE
>Mature_199_residues
MCNLYLVDDDQAVLDSLTWMLNGLGFQPQGFLSADSFLNQVNLHNEGIAVLDVQMPGMDGSALLSLLTKAQSPIAVIMLS
GHGNIAMAVQAIQRGALDFLEKPVDGDKLVVLLEQAKTQTKLNMQRKLAREALSDKLEALTPREHEVMEKVLEGKLNKVI
AAELNIAQRTLELHRQKVMQKMQVSNVAELAFLMGKSTE

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=32.6633165829146, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790299, Length=124, Percent_Identity=32.258064516129, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790437, Length=118, Percent_Identity=33.0508474576271, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788905, Length=119, Percent_Identity=35.2941176470588, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21908; Mature: 21908

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNLYLVDDDQAVLDSLTWMLNGLGFQPQGFLSADSFLNQVNLHNEGIAVLDVQMPGMDG
CCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCH
SALLSLLTKAQSPIAVIMLSGHGNIAMAVQAIQRGALDFLEKPVDGDKLVVLLEQAKTQT
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHH
KLNMQRKLAREALSDKLEALTPREHEVMEKVLEGKLNKVIAAELNIAQRTLELHRQKVMQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMQVSNVAELAFLMGKSTE
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCNLYLVDDDQAVLDSLTWMLNGLGFQPQGFLSADSFLNQVNLHNEGIAVLDVQMPGMDG
CCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCH
SALLSLLTKAQSPIAVIMLSGHGNIAMAVQAIQRGALDFLEKPVDGDKLVVLLEQAKTQT
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHH
KLNMQRKLAREALSDKLEALTPREHEVMEKVLEGKLNKVIAAELNIAQRTLELHRQKVMQ
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMQVSNVAELAFLMGKSTE
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]