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The map label for this gene is seqA [H]

Identifier: 157961876

GI number: 157961876

Start: 2486928

End: 2487536

Strand: Direct

Name: seqA [H]

Synonym: Spea_2055

Alternate gene names: 157961876

Gene position: 2486928-2487536 (Clockwise)

Preceding gene: 157961866

Following gene: 157961877

Centisome position: 48.06

GC content: 42.2

Gene sequence:

>609_bases
ATGCGATCTTTTGCATTTAAGAACACCGATCCGCGTTTCTACTTAAGGAAGATAACCGTTATGAAATATATCGAAGTTGA
TGAAGAGCTTTATCGACACATCGCCAGCAAGACTGAACATATTGGCGAGAGCGCTTCGGATATTTTGCGCCGTATATTGG
GATTAGAAGTTGAATCAGTGGTTCAAGCTGCACCTGAAGAGATTAGTCATCCTAGCCTTGAAGAGTCTTCACCTAAGCCT
ATAAAAGTGACAAAATTGGCAGCTAAGCCAGTTTCTGATTTTAGCAATTTGATTGATGCCGATGCGTTAGCTGCGCAAAA
AGGCGCTGTCGGTCGTTTTCTATTCATTCTAGACACAGTTTATAGAGCATCGACTAAACAGTTTGAGCAGGTATTACAAA
TTCAAGGCCGTGACCGTTTGTATTTTGCAACGTCGAAAGACGCACTGCTAAAAGCCAGTAAGTCAGCTAATCCAAAAGAG
ATTGGCCGCAGTGGTTTTTGGGTGACGACCAACAATAATACCGCGAAGAAGCGCACTATACTTTCGGAAGTGTTGCTGCA
GTTTGGTACTGATGAATCGCAAGTTGCCGATATCATAGAAAGAATTTAA

Upstream 100 bases:

>100_bases
ATAGTTTGCGATCTTTCCTCTCTGGATCGCAGTTTTCATTTAATGACGCTTTATCAAGGCCCTAAACCTCGCTACACTGA
GCTGATAATTCGGGATAGTT

Downstream 100 bases:

>100_bases
CGATATAAAGCGTTAACGTAAAATATATAGACTTACCATTTATGAATTCATAAAGGTAATAGAAGGAGAGGGACTTGGCT
ATACATAAGAGAGCAGGATT

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MRSFAFKNTDPRFYLRKITVMKYIEVDEELYRHIASKTEHIGESASDILRRILGLEVESVVQAAPEEISHPSLEESSPKP
IKVTKLAAKPVSDFSNLIDADALAAQKGAVGRFLFILDTVYRASTKQFEQVLQIQGRDRLYFATSKDALLKASKSANPKE
IGRSGFWVTTNNNTAKKRTILSEVLLQFGTDESQVADIIERI

Sequences:

>Translated_202_residues
MRSFAFKNTDPRFYLRKITVMKYIEVDEELYRHIASKTEHIGESASDILRRILGLEVESVVQAAPEEISHPSLEESSPKP
IKVTKLAAKPVSDFSNLIDADALAAQKGAVGRFLFILDTVYRASTKQFEQVLQIQGRDRLYFATSKDALLKASKSANPKE
IGRSGFWVTTNNNTAKKRTILSEVLLQFGTDESQVADIIERI
>Mature_202_residues
MRSFAFKNTDPRFYLRKITVMKYIEVDEELYRHIASKTEHIGESASDILRRILGLEVESVVQAAPEEISHPSLEESSPKP
IKVTKLAAKPVSDFSNLIDADALAAQKGAVGRFLFILDTVYRASTKQFEQVLQIQGRDRLYFATSKDALLKASKSANPKE
IGRSGFWVTTNNNTAKKRTILSEVLLQFGTDESQVADIIERI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=189, Percent_Identity=38.6243386243386, Blast_Score=120, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 22692; Mature: 22692

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFAFKNTDPRFYLRKITVMKYIEVDEELYRHIASKTEHIGESASDILRRILGLEVESV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
VQAAPEEISHPSLEESSPKPIKVTKLAAKPVSDFSNLIDADALAAQKGAVGRFLFILDTV
HHHCHHHCCCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YRASTKQFEQVLQIQGRDRLYFATSKDALLKASKSANPKEIGRSGFWVTTNNNTAKKRTI
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCHHHHCCCCCEEEECCCCHHHHHH
LSEVLLQFGTDESQVADIIERI
HHHHHHHHCCCHHHHHHHHHHC
>Mature Secondary Structure
MRSFAFKNTDPRFYLRKITVMKYIEVDEELYRHIASKTEHIGESASDILRRILGLEVESV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
VQAAPEEISHPSLEESSPKPIKVTKLAAKPVSDFSNLIDADALAAQKGAVGRFLFILDTV
HHHCHHHCCCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YRASTKQFEQVLQIQGRDRLYFATSKDALLKASKSANPKEIGRSGFWVTTNNNTAKKRTI
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCHHHHCCCCCEEEECCCCHHHHHH
LSEVLLQFGTDESQVADIIERI
HHHHHHHHCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]