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The map label for this gene is adhE [H]

Identifier: 157961865

GI number: 157961865

Start: 2475595

End: 2478210

Strand: Reverse

Name: adhE [H]

Synonym: Spea_2044

Alternate gene names: 157961865

Gene position: 2478210-2475595 (Counterclockwise)

Preceding gene: 157961867

Following gene: 157961862

Centisome position: 47.89

GC content: 46.22

Gene sequence:

>2616_bases
ATGACAGTATCAAATGAGCAAGAACTCAATCTCCTAGTTGAAAAGGTACGAACGGCGCAGCAGACGTTCGCCAACTACTC
CCAGGAGCAAGTCGATAAAATTTTCAGAGCCGCTGCTCTCGCGGCTGCAGACGCGAGGATCTCGCTAGCGAAGATGGCTG
CAAGCGAAACCAAAATGGGTGTAATTGAAGATAAAGTGATTAAAAACCACTTTGCATCCGAATACATCTACAACAAATAT
AAAGACGAAAAAACCTGTGGGATCTTATCTGAAGACCCAACGTTCGGCACTATCACAATCGCTGAGCCCGTCGGTATCAT
CTGCGGTATCGTCCCCACAACTAACCCCACTTCTACAGCAATCTTTAAAGCCTTAATCAGCTTGAAAACCCGTAACGGCA
TTATCTTTTCGCCACATCCTAGAGCAAAAGAATCCACCACAATGGCGGCCCGCATAGTCCTTGAAGCCGCTATAGGCGCA
GGCGCACCTACTGACATTATTGGCTGGATTGATGAGCCATCGGTGGCCTTATCGAATCAATTAATGACTCATGATGACAT
TAATCTCATTCTTGCTACAGGCGGTCCTGGAATGGTAAAAGCGGCTTACTCTTCAGGAAAGCCAGCCATCGGTGTAGGCG
CAGGTAATACGCCGATTGTTATTGATGAAACGGCCGATATTAAACGCGCAGTCAGCTCAATCCTCATGTCTAAAACCTTT
GATAACGGCGTGGTATGCGCTTCAGAGCAAGCTGTTGTGATTGTTGATGAAGTATACGAAGCGGTAAAAGAGCGATTCGC
CAGCCATGGGGGCTATGTATTATCGGTCGCAGAAACTGCTGCTATGCAGAGTGTTATTTTGAAAAATGGTGGGCTCAACG
CCGATATTGTTGGCCAAAGCGCGGCGAGTATTGCCAAGATGGCCGGAATAGAAGTTCATCCTACAACCAAAGTGCTGATA
GGCGAAGTTATTGATATTGATGAATCTGAAGCCTTTGCTCATGAAAAACTCTCCCCTCTGCTCGCTATGTATCGCGCCAA
AGACTTTGCCGACGCCGTAGACAAGGCGGAGGCGTTGGTAACCTTAGGTGGTATCGGCCATACATCAGGTTTATATACCA
ATCAGGACACAGAAATAGAAAGAGTCAAAGCATTCGGTTTTAGAATGAAGACTGCTAGAATTCTAATTAACACCCCCGCT
TCCCAAGGTGGAATTGGCGATCTCTACAACTTTAAGTTAGCACCATCACTCACGTTAGGATGTGGTTCTTGGGGGGGAAA
TTCTATCTCAGAAAATGTTGGCCCGAGCCACTTAATCAACAAGAAAATGGTCGCTAAGAGAGCTGAAAATATGTTGTGGC
ACAAACTCCCCTCATCAATCTATTTCCGCCGCGGCAGCTTACCTATCGCGCTCGAAGAATTAAGCGATAAGAAACGAGCC
CTTATCGTTACTGACAAGTATCTGTTTAATAACGGCTACTGCGATGAAACGATTAAGATTTTAAAAGCACAAGGGCTAGA
AACCGAAGTCTTCTACGAGGTGGAAGCCGATCCAACTATGCATATTGTTAAGCAAGGAGCTAAAGTCGCTCAAAGCTTTC
AACCCGATGTGATTATCGCGCTCGGTGGTGGTTCACCGATGGACGCCGCTAAAATTATCTGGGTAATGTATGAGCATCCA
GATGTCGATTTCGCCGATTTAGCACTGCGCTTTATGGATATCACCAAACGTATCTATAAGTTTCCAAAGCTTGGACGCAA
GGCCAAGATGGTCGCGATTCCGACAACCTCAGGTACGGGCTCAGAAGTCACACCTTTTGCCGTGGTTACCGATGAGCAAA
CAGGGATGAAATACCCAATTGCCGATTATGAATTAACGCCTAACATGGCTATCGTTGACCCTAACTTGGTGATGGATATG
CCAAAGTCCCTCACCGCCTTTGGTGGTATCGATGCGATAACCCACGCATTAGAAGCCTATGTTAGTGTGATGGCCAACGA
ATATTCAGATGGTCAAGCATTACAAGCGCTAGACTTACTGTTTCAGCATCTGCCTGATGCCTATGAACACGGTGCTAATG
CACCCGTTGCCAGAGAAAAGGTTCATAACGGCGCGACCATTGCGGGCATTGCTTTTGCCAACGCTTTTTTAGGCATTTGT
CACTCAATGGCCCACAAGTTAGGAGCTGAATTCCATTTAGCCCATGGTTTGGCCAACGCATTACTTATCAGTAACGTGAT
CCGCTTTAACGCGACAGATCTGCCGACTAAGCAAGCTGCCTTTAGTCAGTATGACAGGCCTAAAGCATTGTGTCGTTATG
CAAAAATTGCAGAATATTTGAAACTCGAAGGAACATTAGGTGAAGGTGTTAGTGATGAAGAAAAAGTCGAAGCGCTCATT
AAAAAAGTTGATGAGCTCAAGGAGACGATTGGCATTCCATGCTCAATCCAAGCAGCAGGCGTCAACGAAGCCGACTTCCT
CGCCAAACTCGACGAACTAGCCGAAGATGCCTTTGATGACCAATGTACAGGCGCTAACCCTCGCTATCCGCTAATTAGCG
AGCTTAAGCAAGTATTGTTGGCAAGTTACTACGGTAATGACTATAGCGATCAATAA

Upstream 100 bases:

>100_bases
GTCGTCTTTGCGTGTAATTTTTCATCATCAAAAAGATTTTAGAGCACTAGAGAATGTTTAGCCAAATACGATTTGGCTAA
ATTTTTTCAGGATTGCGACT

Downstream 100 bases:

>100_bases
CTTGCTTTAATCATTAGAAAGCCCTAACCGAATGGTTAGGGCTTTTTATTGTGGTTCCCCCTAAGGTTGAATTACGACTG
CAGTTTTGCTTGCTTTCGAT

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 871; Mature: 870

Protein sequence:

>871_residues
MTVSNEQELNLLVEKVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAASETKMGVIEDKVIKNHFASEYIYNKY
KDEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNGIIFSPHPRAKESTTMAARIVLEAAIGA
GAPTDIIGWIDEPSVALSNQLMTHDDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
DNGVVCASEQAVVIVDEVYEAVKERFASHGGYVLSVAETAAMQSVILKNGGLNADIVGQSAASIAKMAGIEVHPTTKVLI
GEVIDIDESEAFAHEKLSPLLAMYRAKDFADAVDKAEALVTLGGIGHTSGLYTNQDTEIERVKAFGFRMKTARILINTPA
SQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
LIVTDKYLFNNGYCDETIKILKAQGLETEVFYEVEADPTMHIVKQGAKVAQSFQPDVIIALGGGSPMDAAKIIWVMYEHP
DVDFADLALRFMDITKRIYKFPKLGRKAKMVAIPTTSGTGSEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMDM
PKSLTAFGGIDAITHALEAYVSVMANEYSDGQALQALDLLFQHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGIC
HSMAHKLGAEFHLAHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAKIAEYLKLEGTLGEGVSDEEKVEALI
KKVDELKETIGIPCSIQAAGVNEADFLAKLDELAEDAFDDQCTGANPRYPLISELKQVLLASYYGNDYSDQ

Sequences:

>Translated_871_residues
MTVSNEQELNLLVEKVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAASETKMGVIEDKVIKNHFASEYIYNKY
KDEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNGIIFSPHPRAKESTTMAARIVLEAAIGA
GAPTDIIGWIDEPSVALSNQLMTHDDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
DNGVVCASEQAVVIVDEVYEAVKERFASHGGYVLSVAETAAMQSVILKNGGLNADIVGQSAASIAKMAGIEVHPTTKVLI
GEVIDIDESEAFAHEKLSPLLAMYRAKDFADAVDKAEALVTLGGIGHTSGLYTNQDTEIERVKAFGFRMKTARILINTPA
SQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
LIVTDKYLFNNGYCDETIKILKAQGLETEVFYEVEADPTMHIVKQGAKVAQSFQPDVIIALGGGSPMDAAKIIWVMYEHP
DVDFADLALRFMDITKRIYKFPKLGRKAKMVAIPTTSGTGSEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMDM
PKSLTAFGGIDAITHALEAYVSVMANEYSDGQALQALDLLFQHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGIC
HSMAHKLGAEFHLAHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAKIAEYLKLEGTLGEGVSDEEKVEALI
KKVDELKETIGIPCSIQAAGVNEADFLAKLDELAEDAFDDQCTGANPRYPLISELKQVLLASYYGNDYSDQ
>Mature_870_residues
TVSNEQELNLLVEKVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAASETKMGVIEDKVIKNHFASEYIYNKYK
DEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNGIIFSPHPRAKESTTMAARIVLEAAIGAG
APTDIIGWIDEPSVALSNQLMTHDDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTFD
NGVVCASEQAVVIVDEVYEAVKERFASHGGYVLSVAETAAMQSVILKNGGLNADIVGQSAASIAKMAGIEVHPTTKVLIG
EVIDIDESEAFAHEKLSPLLAMYRAKDFADAVDKAEALVTLGGIGHTSGLYTNQDTEIERVKAFGFRMKTARILINTPAS
QGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRAL
IVTDKYLFNNGYCDETIKILKAQGLETEVFYEVEADPTMHIVKQGAKVAQSFQPDVIIALGGGSPMDAAKIIWVMYEHPD
VDFADLALRFMDITKRIYKFPKLGRKAKMVAIPTTSGTGSEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMDMP
KSLTAFGGIDAITHALEAYVSVMANEYSDGQALQALDLLFQHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGICH
SMAHKLGAEFHLAHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAKIAEYLKLEGTLGEGVSDEEKVEALIK
KVDELKETIGIPCSIQAAGVNEADFLAKLDELAEDAFDDQCTGANPRYPLISELKQVLLASYYGNDYSDQ

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=320, Percent_Identity=27.1875, Blast_Score=84, Evalue=8e-16,
Organism=Escherichia coli, GI1787493, Length=870, Percent_Identity=75.632183908046, Blast_Score=1335, Evalue=0.0,
Organism=Escherichia coli, GI48994951, Length=398, Percent_Identity=35.427135678392, Blast_Score=202, Evalue=7e-53,
Organism=Escherichia coli, GI1789163, Length=411, Percent_Identity=33.0900243309002, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI87082107, Length=386, Percent_Identity=31.8652849740933, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1788797, Length=373, Percent_Identity=31.0991957104558, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1789386, Length=393, Percent_Identity=25.1908396946565, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17537053, Length=282, Percent_Identity=27.3049645390071, Blast_Score=108, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321181, Length=396, Percent_Identity=33.0808080808081, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24657991, Length=319, Percent_Identity=26.0188087774295, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 93893; Mature: 93762

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSNEQELNLLVEKVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAASETKMG
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VIEDKVIKNHFASEYIYNKYKDEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCHHHH
IFKALISLKTRNGIIFSPHPRAKESTTMAARIVLEAAIGAGAPTDIIGWIDEPSVALSNQ
HHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHEECCCCCCHHHHCC
LMTHDDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
CEECCCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
DNGVVCASEQAVVIVDEVYEAVKERFASHGGYVLSVAETAAMQSVILKNGGLNADIVGQS
CCCEEEECCCCEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCEECCCH
AASIAKMAGIEVHPTTKVLIGEVIDIDESEAFAHEKLSPLLAMYRAKDFADAVDKAEALV
HHHHHHHCCCEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TLGGIGHTSGLYTNQDTEIERVKAFGFRMKTARILINTPASQGGIGDLYNFKLAPSLTLG
EECCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEHHHCCCCCCE
LIVTDKYLFNNGYCDETIKILKAQGLETEVFYEVEADPTMHIVKQGAKVAQSFQPDVIIA
EEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEE
LGGGSPMDAAKIIWVMYEHPDVDFADLALRFMDITKRIYKFPKLGRKAKMVAIPTTSGTG
ECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
SEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMDMPKSLTAFGGIDAITHALEAY
CCCCCEEEEECCCCCCCCCCCCCEECCCEEEECCHHHHHCCHHHHHHCCHHHHHHHHHHH
VSVMANEYSDGQALQALDLLFQHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGIC
HHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHH
HSMAHKLGAEFHLAHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAKIAEYL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
KLEGTLGEGVSDEEKVEALIKKVDELKETIGIPCSIQAAGVNEADFLAKLDELAEDAFDD
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCC
QCTGANPRYPLISELKQVLLASYYGNDYSDQ
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVSNEQELNLLVEKVRTAQQTFANYSQEQVDKIFRAAALAAADARISLAKMAASETKMG
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VIEDKVIKNHFASEYIYNKYKDEKTCGILSEDPTFGTITIAEPVGIICGIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCHHHH
IFKALISLKTRNGIIFSPHPRAKESTTMAARIVLEAAIGAGAPTDIIGWIDEPSVALSNQ
HHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHEECCCCCCHHHHCC
LMTHDDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
CEECCCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
DNGVVCASEQAVVIVDEVYEAVKERFASHGGYVLSVAETAAMQSVILKNGGLNADIVGQS
CCCEEEECCCCEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCEECCCH
AASIAKMAGIEVHPTTKVLIGEVIDIDESEAFAHEKLSPLLAMYRAKDFADAVDKAEALV
HHHHHHHCCCEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TLGGIGHTSGLYTNQDTEIERVKAFGFRMKTARILINTPASQGGIGDLYNFKLAPSLTLG
EECCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNSISENVGPSHLINKKMVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEHHHCCCCCCE
LIVTDKYLFNNGYCDETIKILKAQGLETEVFYEVEADPTMHIVKQGAKVAQSFQPDVIIA
EEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEE
LGGGSPMDAAKIIWVMYEHPDVDFADLALRFMDITKRIYKFPKLGRKAKMVAIPTTSGTG
ECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
SEVTPFAVVTDEQTGMKYPIADYELTPNMAIVDPNLVMDMPKSLTAFGGIDAITHALEAY
CCCCCEEEEECCCCCCCCCCCCCEECCCEEEECCHHHHHCCHHHHHHCCHHHHHHHHHHH
VSVMANEYSDGQALQALDLLFQHLPDAYEHGANAPVAREKVHNGATIAGIAFANAFLGIC
HHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHH
HSMAHKLGAEFHLAHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAKIAEYL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
KLEGTLGEGVSDEEKVEALIKKVDELKETIGIPCSIQAAGVNEADFLAKLDELAEDAFDD
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCC
QCTGANPRYPLISELKQVLLASYYGNDYSDQ
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]