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The map label for this gene is fumB [H]

Identifier: 157961859

GI number: 157961859

Start: 2465456

End: 2466976

Strand: Direct

Name: fumB [H]

Synonym: Spea_2038

Alternate gene names: 157961859

Gene position: 2465456-2466976 (Clockwise)

Preceding gene: 157961856

Following gene: 157961860

Centisome position: 47.65

GC content: 48.59

Gene sequence:

>1521_bases
GTGAGCAAAGAAGTCCCTGTAATCAAGCAAGCCGATCTCATTGAAAGCGTTGCTGATGCCCTACAATATATCTCTTATTA
TCACCCAAAAGACTTTGTTGATTCTATGGCTGAAGCCTATGAACGCGAACAAAGCTCTGCAGCAAAAGACGCGATTGCAC
AAATTCTTATCAATTCTCGTATGTCAGCCGAAGGTAAACGTCCACTTTGCCAAGATACCGGTATTGTGACCTGTTTCGTT
AAGATTGGTATGGGCGTGCAATGGGATAAGACAGATCTAACCGTTCAAGAGATGGTTGATGAAGGCGTACGCCGTGCTTA
TACCAATCCAGATAATCCTCTTCGTGCATCGATTGTCGCCGATCCTGCTGGCGGCCGTAAAAACACCAAAGACAACACGC
CGTCAGTTGTGCATGTTGAGATGGTTCCTGGTAACCATGTTGACGTGGCGATTGCAGCTAAGGGCGGTGGATCTGAAAAC
AAATCTAAGATGGTTATGCTTAACCCATCAGATGATATTGCAGCTTGGGTTGAGAAAACGCTTCCTACTATGGGCGCGGG
TTGGTGCCCACCAGGAATGCTTGGTATAGGTATTGGCGGTACCGCCGAAAAAGCGGCGGTAATGGCTAAAGAGTCGCTGA
TGGATCCTGTCGATATTCATGAGCTTCAAGCTCGCGGCGCCCAAACTACCGATGAAAAGTTACGCCTAGAAATTTTTGAG
CGTGCTAATAAGCTAGGTATTGGCGCGCAAGGCTTAGGTGGTGTAACGACTGTACTGGATATCAAGATTAAGTCAGCCCC
TACACATGCGGCCTCTAAGCCTGTAGTGATGATCCCAAATTGTGCCGCGACTCGTCACGTGCATTTTCACCTAGATGGCA
CCGGTCCCGCGCTTCTAACGCCTCCATCATTGGAAGACTGGCCTGAAATTACTTGGGAAGTCGGGGAGAGTGTGCGCCGT
GTCGATCTTAACACTGTGACCCAAGCCGAAATGGAGCAGTGGAAGAGTGGTGACACCTTGTTGCTTAATGGCAAGATGCT
GACCGGTCGTGACGCGGCTCATAAGCGTATTCAAAGTTTAATTGAGAGCGGAGAAGGCTTACCAGAAGGTGTCGACTTTA
AAGGCAAGTTTATCTACTACGTCGGTCCTGTCGATCCAGTCGGTGATGAAGTGGTGGGTCCTGCTGGCCCGACTACGGCA
ACCCGGATGGATAAGTTTACCGATCTAATGCTAGATAAAACAGGTTTGATGGGCATGATCGGTAAAGCTGAGCGTGGTCC
TGCTACCGTGGAATCAATCAAGAAACATAAGTCTTGTTACTTGATGGCCGTTGGCGGCGCTGCGTACCTAGTTTCTAAGG
CGATTAAGCACTCTCGCGTTGTCGCTTTTGAGGAACTCGGTATGGAAGCTATCTATGAGTTCGACGTTAAGGATATGCCT
GTTACTGTCGCTGTAGACTCAGAAGGCGTTAATGCCCATGAAACGGGTCCAGCAATCTGGAAAGTTAAGCTAGAAGGCTA
A

Upstream 100 bases:

>100_bases
CAAAACATGTGAGCTTTAAAGGCGACTGAAGTTAATCATGCTAGCAATTGCCAGCTTAAGTTCAGCCAATTAATAAGTGT
CTGCTAAAAAGGAAGTGTCT

Downstream 100 bases:

>100_bases
TACAGAGGGCTAATACAGAAGGCTAGTACAAAGAGTTAATACAAAAGGCAAAGCTAGAAAGCCGAAAATAAAAGCCTACT
TGTAAACTTTGAGTGAGTAA

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSSAAKDAIAQILINSRMSAEGKRPLCQDTGIVTCFV
KIGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVADPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSEN
KSKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAQTTDEKLRLEIFE
RANKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPALLTPPSLEDWPEITWEVGESVRR
VDLNTVTQAEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSLIESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTA
TRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKHSRVVAFEELGMEAIYEFDVKDMP
VTVAVDSEGVNAHETGPAIWKVKLEG

Sequences:

>Translated_506_residues
MSKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSSAAKDAIAQILINSRMSAEGKRPLCQDTGIVTCFV
KIGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVADPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSEN
KSKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAQTTDEKLRLEIFE
RANKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPALLTPPSLEDWPEITWEVGESVRR
VDLNTVTQAEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSLIESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTA
TRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKHSRVVAFEELGMEAIYEFDVKDMP
VTVAVDSEGVNAHETGPAIWKVKLEG
>Mature_505_residues
SKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSSAAKDAIAQILINSRMSAEGKRPLCQDTGIVTCFVK
IGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVADPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSENK
SKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAQTTDEKLRLEIFER
ANKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPALLTPPSLEDWPEITWEVGESVRRV
DLNTVTQAEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSLIESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTAT
RMDKFTDLMLDKTGLMGMIGKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKHSRVVAFEELGMEAIYEFDVKDMPV
TVAVDSEGVNAHETGPAIWKVKLEG

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=472, Percent_Identity=33.8983050847458, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1787897, Length=471, Percent_Identity=34.3949044585987, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1789442, Length=280, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789443, Length=175, Percent_Identity=34.8571428571429, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54432; Mature: 54301

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSSAAKDAIAQILINSR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MSAEGKRPLCQDTGIVTCFVKIGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVA
CCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
DPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSENKSKMVMLNPSDDIAAWVEKT
CCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHH
LPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAQTTDEKLRLEIFE
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHH
RANKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPALLT
HHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCEEEC
PPSLEDWPEITWEVGESVRRVDLNTVTQAEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSL
CCCCCCHHHHHHHHCCHHHEECCHHHHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHH
IESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTDLMLDKTGLMGMI
HHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
GKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKHSRVVAFEELGMEAIYEFDVKDMP
CCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHEEECCCCCCC
VTVAVDSEGVNAHETGPAIWKVKLEG
EEEEECCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
SKEVPVIKQADLIESVADALQYISYYHPKDFVDSMAEAYEREQSSAAKDAIAQILINSR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MSAEGKRPLCQDTGIVTCFVKIGMGVQWDKTDLTVQEMVDEGVRRAYTNPDNPLRASIVA
CCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
DPAGGRKNTKDNTPSVVHVEMVPGNHVDVAIAAKGGGSENKSKMVMLNPSDDIAAWVEKT
CCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHH
LPTMGAGWCPPGMLGIGIGGTAEKAAVMAKESLMDPVDIHELQARGAQTTDEKLRLEIFE
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHH
RANKLGIGAQGLGGVTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGTGPALLT
HHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCCCEEEC
PPSLEDWPEITWEVGESVRRVDLNTVTQAEMEQWKSGDTLLLNGKMLTGRDAAHKRIQSL
CCCCCCHHHHHHHHCCHHHEECCHHHHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHH
IESGEGLPEGVDFKGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTDLMLDKTGLMGMI
HHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
GKAERGPATVESIKKHKSCYLMAVGGAAYLVSKAIKHSRVVAFEELGMEAIYEFDVKDMP
CCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHEEECCCCCCC
VTVAVDSEGVNAHETGPAIWKVKLEG
EEEEECCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]