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The map label for this gene is hydA [H]

Identifier: 157961854

GI number: 157961854

Start: 2459679

End: 2460815

Strand: Reverse

Name: hydA [H]

Synonym: Spea_2033

Alternate gene names: 157961854

Gene position: 2460815-2459679 (Counterclockwise)

Preceding gene: 157961855

Following gene: 157961853

Centisome position: 47.56

GC content: 49.52

Gene sequence:

>1137_bases
ATGGAAACACATGAAGCCCTTTACCAACAAGGTGTGGCGCGAATGGAACAACTTCGCAAGCTACAGCCCCGTACTGGTGA
AACGCTACAGCAAAAAATGGAACAAAATGGCATTACTCGTCGCGACTTTATGAAGTGGAGCGCTGCCGTTACCGCCATGT
TAGCTTTACCCTTACCCTTTAGTACTTTAGTTGCTGAAGCTGCCGAACTTGCAGACCGAGTCCCGCTTGTTTGGTTACAC
ATGGCCGAATGTACAGGCTGCAGTGAGTCGCTAATCCGTACCGACACACCTAACTTAGATACCCTAATCTTTAATCATGT
CTCTCTTGAGTACCATGAAACCTTAATGGCCGCAGCTGGCTGGCAAGCAGAAGAAAATCTTGAGCACGCATTATCAGCCT
ACAAGGGTAACTATCTGCTTGCTGTTGAGGGCGCAGTTCCTACTGCAAATAATGGCGCTTACTTAACGGTGGGCTGTAAA
GGCCATACTGGCCTGCATATCATTAAAGAAGCTGCCGAAGGCGCTGCAGCAATTATTTCTGTCGGTACGTGCGCTGCCTT
TGGCGGTATTCAAGCTGCCGCACCTAACCCAACAGGGGCCAAAGGTGTTTACGAAGTCGTTGATAGAACCGTTATTAACT
TAGGTGGCTGCCCACCAAGTGAGAAAAACATCGTCGGCACGCTGATGTATTTCATCATGTTTGGTAAGTTACCTGCCCTT
GATATGTTTAACCGTCCTAAGTGGGCATACGGTGCTCGCGTTCACGATAACTGCGAGCGTCGTGGCCGTTTTGATGCTGG
CGAGTTTGTTGAAGAGTTCGGCGACCAGGGCGCAAAAGATGGCTATTGCCTCTATAAGGTTGGCTGTAAGGGTCCTTACA
CCTATAACAACTGTCCAACCGAAAGATTCAACCACCATACCAGTTGGCCAGTGCTTGCCGGTCACGGCTGTATCGGCTGC
TCTGAGCCAAACTTCTGGGACGATATGGCTGATTTTGAGAAACCTTTAGGAAGGCAATTACTGCATGGTCTAGATGCGAC
AAGCGACACTGTCGGCGCGGTCATTCTAGGTGCAACCGCAGTCGGAATTGGCGCCCACGCTGTCGCCAGTATTTTTGCCA
AATCTGAAGAGGAATAA

Upstream 100 bases:

>100_bases
AAGAACTCAAAATCTTGACTCATATCAATAAGACCCTAGCTTTTGAGGCCTAGACTGTTTTCGTTGAGCAAATTCCTTAA
AAACAAAAAAAGGTTTAACC

Downstream 100 bases:

>100_bases
TCATGAGTAAACGTGTAGTCATAGATCCAATCACTCGTATTGAAGGACATCTACGAGTCGAAGTCGAAGTCGACGAAAAT
AATGTTATTCAGAAAGCCTG

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
METHEALYQQGVARMEQLRKLQPRTGETLQQKMEQNGITRRDFMKWSAAVTAMLALPLPFSTLVAEAAELADRVPLVWLH
MAECTGCSESLIRTDTPNLDTLIFNHVSLEYHETLMAAAGWQAEENLEHALSAYKGNYLLAVEGAVPTANNGAYLTVGCK
GHTGLHIIKEAAEGAAAIISVGTCAAFGGIQAAAPNPTGAKGVYEVVDRTVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDQGAKDGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCIGC
SEPNFWDDMADFEKPLGRQLLHGLDATSDTVGAVILGATAVGIGAHAVASIFAKSEEE

Sequences:

>Translated_378_residues
METHEALYQQGVARMEQLRKLQPRTGETLQQKMEQNGITRRDFMKWSAAVTAMLALPLPFSTLVAEAAELADRVPLVWLH
MAECTGCSESLIRTDTPNLDTLIFNHVSLEYHETLMAAAGWQAEENLEHALSAYKGNYLLAVEGAVPTANNGAYLTVGCK
GHTGLHIIKEAAEGAAAIISVGTCAAFGGIQAAAPNPTGAKGVYEVVDRTVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDQGAKDGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCIGC
SEPNFWDDMADFEKPLGRQLLHGLDATSDTVGAVILGATAVGIGAHAVASIFAKSEEE
>Mature_378_residues
METHEALYQQGVARMEQLRKLQPRTGETLQQKMEQNGITRRDFMKWSAAVTAMLALPLPFSTLVAEAAELADRVPLVWLH
MAECTGCSESLIRTDTPNLDTLIFNHVSLEYHETLMAAAGWQAEENLEHALSAYKGNYLLAVEGAVPTANNGAYLTVGCK
GHTGLHIIKEAAEGAAAIISVGTCAAFGGIQAAAPNPTGAKGVYEVVDRTVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDQGAKDGYCLYKVGCKGPYTYNNCPTERFNHHTSWPVLAGHGCIGC
SEPNFWDDMADFEKPLGRQLLHGLDATSDTVGAVILGATAVGIGAHAVASIFAKSEEE

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=332, Percent_Identity=43.6746987951807, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1789371, Length=292, Percent_Identity=48.6301369863014, Blast_Score=267, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 40916; Mature: 40916

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METHEALYQQGVARMEQLRKLQPRTGETLQQKMEQNGITRRDFMKWSAAVTAMLALPLPF
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
STLVAEAAELADRVPLVWLHMAECTGCSESLIRTDTPNLDTLIFNHVSLEYHETLMAAAG
HHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLEHALSAYKGNYLLAVEGAVPTANNGAYLTVGCKGHTGLHIIKEAAEGAAAIIS
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEHH
VGTCAAFGGIQAAAPNPTGAKGVYEVVDRTVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDQGAKDGYCLYKVGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCIGCSEPNFWDDMADFEKPLGRQLLHGLDATSDTVGAVILGATA
HHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VGIGAHAVASIFAKSEEE
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METHEALYQQGVARMEQLRKLQPRTGETLQQKMEQNGITRRDFMKWSAAVTAMLALPLPF
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
STLVAEAAELADRVPLVWLHMAECTGCSESLIRTDTPNLDTLIFNHVSLEYHETLMAAAG
HHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
WQAEENLEHALSAYKGNYLLAVEGAVPTANNGAYLTVGCKGHTGLHIIKEAAEGAAAIIS
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEHH
VGTCAAFGGIQAAAPNPTGAKGVYEVVDRTVINLGGCPPSEKNIVGTLMYFIMFGKLPAL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCH
DMFNRPKWAYGARVHDNCERRGRFDAGEFVEEFGDQGAKDGYCLYKVGCKGPYTYNNCPT
HHCCCCCCCCCCCHHHCHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCH
ERFNHHTSWPVLAGHGCIGCSEPNFWDDMADFEKPLGRQLLHGLDATSDTVGAVILGATA
HHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VGIGAHAVASIFAKSEEE
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]