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The map label for this gene is hydB [H]

Identifier: 157961853

GI number: 157961853

Start: 2457973

End: 2459676

Strand: Reverse

Name: hydB [H]

Synonym: Spea_2032

Alternate gene names: 157961853

Gene position: 2459676-2457973 (Counterclockwise)

Preceding gene: 157961854

Following gene: 157961852

Centisome position: 47.53

GC content: 47.01

Gene sequence:

>1704_bases
ATGAGTAAACGTGTAGTCATAGATCCAATCACTCGTATTGAAGGACATCTACGAGTCGAAGTCGAAGTCGACGAAAATAA
TGTTATTCAGAAAGCCTGGTCATCTTCTACTTTGTGGCGCGGCATTGAAGTCATTTTAAAAGGCCGTACACCTATGGATG
TTGGCCTCATCGTTCAACGTATTTGCGGCGTGTGTACCTATTCACACTATCGCTGCGGTACAGAAGCGGTAGAAAATGCA
TTGGGCGTAAAAATTCCACTCAATGCAAAATACCTTAGAAGCTTAATGCAAACATCGCTTTATATGCATGACCATATCGT
GCATTTCTATCACCTTCACGGCCTAGATTGGGTCGATGTAGTTTCAGCATTGAGCGCCGATCCAGCGCTAGCCGCGCAAG
AAGCAATGAAGTACACCGCTAACCCTATCGCTGCAGGTGAAGGCGATCTTCGCGCAGTACAAGAGCGTGTAGCTGGTCTT
GTTGAAACTGGTAAGTTAGGCCCATTCGCAAATGCATACTGGGGTAATGGCACCTATAAGTTCACCCCAGAGCAAAACCT
AATTGCTCTATCGCATTACTTAAAAGCACTAGAAATTCAGCGTATCGCAGCAGAGATGCTAGCTATCTTCGGTGGTAAGT
CGCCTCACCCACAATCAATCGTTGTCGGTGGTGTCACTTCAGTACGCGACATGCTAAGCCCTGCTCGCTTGCAAGAGTGG
AAGCAAAAACATCAAATCGTTCAAGACTTTATTATCCGCGCTTATAAAGCAGACATCGTGATGGCGGCTGCTGCATTTGG
CGCTGAACCAAGTGTTCTCGGTGGCGTAAACGTTAAGAACTTCCTCGCAACTGAAGACTTCCTGTTAAGTGATGGCCAGT
ACATGTTTAACCAAGGCGTTATCATGAATGGCGATCTTGGAAATGTGAGTGACTTAGATCCAGACATGATTGCAGAAGAT
GCGACTCACGCTTGGTATAAGGCTGACGGCCCACAGCATCCATATGACGGTACAACAGTTCCTGATTACACAGGCTTTGT
TGAAAGAGATACCGTTTACGGTAAGTTACCGACTGTCGATGGCGACGGTAAGTATACTTGGGTTAAGTCACCACGTTACA
ACGGTGAACCTGTCGAAGTGGGCCCACTGGCAAGCCTATTAGTTAGCTATGCTCGTGGTAACCAAGTCGTTGTTGACTCA
GTAAATGGTTTACTGGAAGCGACTGGCCTCCCCGTTGGAGCATTATTCACCACATTGGGCCGCACCGCCGCACGTATGCT
GCAAACGGTTATCGTTGCCGAAGAGGGTCTTAAGACATTCGATGCAATGCTAACCAACATGCAGTCGGACGAAGCCACTT
ACGTGAAACCTGAGATAGATTCCAGCAAAGAGTATGTTGGCCATGCCATGATTGAAGCGCCGCGCGGCATGCTTAGTCAC
TGGATCCGCATCAAGGGGGGCTTAGTTGAGAACTACCAAGCGGTAGTGCCGACAACTTGGAATGCGGGTCCCGTAGATGC
ACAGGGCAAAATGGGACCTTATGAAGCCTCACTTATTGGCTTAAAGCTTGAAGATCCTACTAAGCCTTTAGAAGTTATCC
GTATTATTCACTCTTTTGACCCTTGTATGGCGTGTGCTGTACACGTAATGGATTACAAAGGCCAAAACCTAGGTGAATTT
AAAATTGATCCCAACGGATTTTAA

Upstream 100 bases:

>100_bases
CAAGCGACACTGTCGGCGCGGTCATTCTAGGTGCAACCGCAGTCGGAATTGGCGCCCACGCTGTCGCCAGTATTTTTGCC
AAATCTGAAGAGGAATAATC

Downstream 100 bases:

>100_bases
TCAAGGGGGATAGTATGGCAACTCAATCTGCGCCCTACCAAAGGGAACTGATCTTTGGTGCTGCAATAAGGATATTTCAC
TGGCTAAGAGCCTTAGCCAT

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSKRVVIDPITRIEGHLRVEVEVDENNVIQKAWSSSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENA
LGVKIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDVVSALSADPALAAQEAMKYTANPIAAGEGDLRAVQERVAGL
VETGKLGPFANAYWGNGTYKFTPEQNLIALSHYLKALEIQRIAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEW
KQKHQIVQDFIIRAYKADIVMAAAAFGAEPSVLGGVNVKNFLATEDFLLSDGQYMFNQGVIMNGDLGNVSDLDPDMIAED
ATHAWYKADGPQHPYDGTTVPDYTGFVERDTVYGKLPTVDGDGKYTWVKSPRYNGEPVEVGPLASLLVSYARGNQVVVDS
VNGLLEATGLPVGALFTTLGRTAARMLQTVIVAEEGLKTFDAMLTNMQSDEATYVKPEIDSSKEYVGHAMIEAPRGMLSH
WIRIKGGLVENYQAVVPTTWNAGPVDAQGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFDPCMACAVHVMDYKGQNLGEF
KIDPNGF

Sequences:

>Translated_567_residues
MSKRVVIDPITRIEGHLRVEVEVDENNVIQKAWSSSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENA
LGVKIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDVVSALSADPALAAQEAMKYTANPIAAGEGDLRAVQERVAGL
VETGKLGPFANAYWGNGTYKFTPEQNLIALSHYLKALEIQRIAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEW
KQKHQIVQDFIIRAYKADIVMAAAAFGAEPSVLGGVNVKNFLATEDFLLSDGQYMFNQGVIMNGDLGNVSDLDPDMIAED
ATHAWYKADGPQHPYDGTTVPDYTGFVERDTVYGKLPTVDGDGKYTWVKSPRYNGEPVEVGPLASLLVSYARGNQVVVDS
VNGLLEATGLPVGALFTTLGRTAARMLQTVIVAEEGLKTFDAMLTNMQSDEATYVKPEIDSSKEYVGHAMIEAPRGMLSH
WIRIKGGLVENYQAVVPTTWNAGPVDAQGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFDPCMACAVHVMDYKGQNLGEF
KIDPNGF
>Mature_566_residues
SKRVVIDPITRIEGHLRVEVEVDENNVIQKAWSSSTLWRGIEVILKGRTPMDVGLIVQRICGVCTYSHYRCGTEAVENAL
GVKIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDVVSALSADPALAAQEAMKYTANPIAAGEGDLRAVQERVAGLV
ETGKLGPFANAYWGNGTYKFTPEQNLIALSHYLKALEIQRIAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEWK
QKHQIVQDFIIRAYKADIVMAAAAFGAEPSVLGGVNVKNFLATEDFLLSDGQYMFNQGVIMNGDLGNVSDLDPDMIAEDA
THAWYKADGPQHPYDGTTVPDYTGFVERDTVYGKLPTVDGDGKYTWVKSPRYNGEPVEVGPLASLLVSYARGNQVVVDSV
NGLLEATGLPVGALFTTLGRTAARMLQTVIVAEEGLKTFDAMLTNMQSDEATYVKPEIDSSKEYVGHAMIEAPRGMLSHW
IRIKGGLVENYQAVVPTTWNAGPVDAQGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFDPCMACAVHVMDYKGQNLGEFK
IDPNGF

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=584, Percent_Identity=43.4931506849315, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI1787207, Length=581, Percent_Identity=39.5869191049914, Blast_Score=415, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 62113; Mature: 61982

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRVVIDPITRIEGHLRVEVEVDENNVIQKAWSSSTLWRGIEVILKGRTPMDVGLIVQR
CCCCEEECCHHHCCCEEEEEEEECCCCHHHHHHCCCHHHHHHHHEEECCCCCHHHHHHHH
ICGVCTYSHYRCGTEAVENALGVKIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDV
HHHHHCCCHHCCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHEEEHHCCCCCHHHH
VSALSADPALAAQEAMKYTANPIAAGEGDLRAVQERVAGLVETGKLGPFANAYWGNGTYK
HHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEE
FTPEQNLIALSHYLKALEIQRIAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEW
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCHHHHHHH
KQKHQIVQDFIIRAYKADIVMAAAAFGAEPSVLGGVNVKNFLATEDFLLSDGQYMFNQGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHCCCCHHCCCCCEECCCCE
IMNGDLGNVSDLDPDMIAEDATHAWYKADGPQHPYDGTTVPDYTGFVERDTVYGKLPTVD
EEECCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEECCCEEEECCCCC
GDGKYTWVKSPRYNGEPVEVGPLASLLVSYARGNQVVVDSVNGLLEATGLPVGALFTTLG
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCHHHHHHHHH
RTAARMLQTVIVAEEGLKTFDAMLTNMQSDEATYVKPEIDSSKEYVGHAMIEAPRGMLSH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHH
WIRIKGGLVENYQAVVPTTWNAGPVDAQGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFD
HHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCC
PCMACAVHVMDYKGQNLGEFKIDPNGF
HHHHHHHHHHHCCCCCCCEEEECCCCC
>Mature Secondary Structure 
SKRVVIDPITRIEGHLRVEVEVDENNVIQKAWSSSTLWRGIEVILKGRTPMDVGLIVQR
CCCEEECCHHHCCCEEEEEEEECCCCHHHHHHCCCHHHHHHHHEEECCCCCHHHHHHHH
ICGVCTYSHYRCGTEAVENALGVKIPLNAKYLRSLMQTSLYMHDHIVHFYHLHGLDWVDV
HHHHHCCCHHCCCHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHEEEHHCCCCCHHHH
VSALSADPALAAQEAMKYTANPIAAGEGDLRAVQERVAGLVETGKLGPFANAYWGNGTYK
HHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEE
FTPEQNLIALSHYLKALEIQRIAAEMLAIFGGKSPHPQSIVVGGVTSVRDMLSPARLQEW
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCHHHHHHH
KQKHQIVQDFIIRAYKADIVMAAAAFGAEPSVLGGVNVKNFLATEDFLLSDGQYMFNQGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHCCCCHHCCCCCEECCCCE
IMNGDLGNVSDLDPDMIAEDATHAWYKADGPQHPYDGTTVPDYTGFVERDTVYGKLPTVD
EEECCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEECCCEEEECCCCC
GDGKYTWVKSPRYNGEPVEVGPLASLLVSYARGNQVVVDSVNGLLEATGLPVGALFTTLG
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCHHHHHHHHH
RTAARMLQTVIVAEEGLKTFDAMLTNMQSDEATYVKPEIDSSKEYVGHAMIEAPRGMLSH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHH
WIRIKGGLVENYQAVVPTTWNAGPVDAQGKMGPYEASLIGLKLEDPTKPLEVIRIIHSFD
HHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCC
PCMACAVHVMDYKGQNLGEFKIDPNGF
HHHHHHHHHHHCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]