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The map label for this gene is polC [H]

Identifier: 157961820

GI number: 157961820

Start: 2422912

End: 2423559

Strand: Reverse

Name: polC [H]

Synonym: Spea_1999

Alternate gene names: 157961820

Gene position: 2423559-2422912 (Counterclockwise)

Preceding gene: 157961822

Following gene: 157961818

Centisome position: 46.84

GC content: 44.91

Gene sequence:

>648_bases
ATGATTAAATTGAGCTATAGCAATCCAATCTTATGCCGCCCAGCCGACACGGTTATCGTACTCGACTTCGAAACCACAGG
TTTATCTCCAACTCAGGGCGATCGAGCGATTGAAATCGGTGCCGTAATGCTAAAAGATGGCAAAGTCGTCGATCGGTTTC
AGCAATTAATGAATCCAGGCTTTCGGATCAGTGGTTTTATCGAAAGCTATACCGGGATCAGTAATGCTATGTTAGCCGAT
GCCCCCTCGTGTGAGGAGGTCATGGCACAATTTGCTGATTTTATCGGTGATTATAACTTGGTCGCCCACAATGCCTCTTT
CGATCAACGTTTTTTGGATGCTGAACTTAAGCGCATAAACTGTCACTACCTTGGCCAATTTGCCTGTTCCATGCTTATTG
CACGCAGGCTTTATCAAGCAGCTCCAAACCATAAACTCGGCAGCTTAGTGAGCTATAAGAATATCCAACATGACGGCGTG
TTTCACCGCGCCCTAGCGGATAGTGAAATGACTGCTAGCCTCTGGCTAGCCATGCTTGAAGATCTACAGCAAGACTATCA
ATTACACAGCCCAGACTTTGGTCTACTACAAAGTATTGCTAAACAAACTAAAGCCAATATTAATCAATATCTACAACGCC
AAGGCTGA

Upstream 100 bases:

>100_bases
GCAGCTATGAATTAAGTAAGATATAGGCTGCATATTAAGATACAAAGCAGATTGACTGAAATGATGGGATGAATACAATA
CCAAGAAACAATACAGATGA

Downstream 100 bases:

>100_bases
ACGATGTGTGTCCTGTATAACGCTTGTTATACAGGACATGTAAAAATCAGGCAGTAGTTTCTTTAACATACAGGCTTTGC
TAGCTCCAAATGAATAAAAT

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIKLSYSNPILCRPADTVIVLDFETTGLSPTQGDRAIEIGAVMLKDGKVVDRFQQLMNPGFRISGFIESYTGISNAMLAD
APSCEEVMAQFADFIGDYNLVAHNASFDQRFLDAELKRINCHYLGQFACSMLIARRLYQAAPNHKLGSLVSYKNIQHDGV
FHRALADSEMTASLWLAMLEDLQQDYQLHSPDFGLLQSIAKQTKANINQYLQRQG

Sequences:

>Translated_215_residues
MIKLSYSNPILCRPADTVIVLDFETTGLSPTQGDRAIEIGAVMLKDGKVVDRFQQLMNPGFRISGFIESYTGISNAMLAD
APSCEEVMAQFADFIGDYNLVAHNASFDQRFLDAELKRINCHYLGQFACSMLIARRLYQAAPNHKLGSLVSYKNIQHDGV
FHRALADSEMTASLWLAMLEDLQQDYQLHSPDFGLLQSIAKQTKANINQYLQRQG
>Mature_215_residues
MIKLSYSNPILCRPADTVIVLDFETTGLSPTQGDRAIEIGAVMLKDGKVVDRFQQLMNPGFRISGFIESYTGISNAMLAD
APSCEEVMAQFADFIGDYNLVAHNASFDQRFLDAELKRINCHYLGQFACSMLIARRLYQAAPNHKLGSLVSYKNIQHDGV
FHRALADSEMTASLWLAMLEDLQQDYQLHSPDFGLLQSIAKQTKANINQYLQRQG

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2176

COG function: function code L; DNA polymerase III, alpha subunit (gram-positive type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788149, Length=161, Percent_Identity=36.6459627329193, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786409, Length=170, Percent_Identity=31.1764705882353, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR023223
- InterPro:   IPR016027
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 24072; Mature: 24072

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLSYSNPILCRPADTVIVLDFETTGLSPTQGDRAIEIGAVMLKDGKVVDRFQQLMNPG
CEEECCCCCEEECCCCEEEEEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCC
FRISGFIESYTGISNAMLADAPSCEEVMAQFADFIGDYNLVAHNASFDQRFLDAELKRIN
CEEHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHH
CHYLGQFACSMLIARRLYQAAPNHKLGSLVSYKNIQHDGVFHRALADSEMTASLWLAMLE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHH
DLQQDYQLHSPDFGLLQSIAKQTKANINQYLQRQG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKLSYSNPILCRPADTVIVLDFETTGLSPTQGDRAIEIGAVMLKDGKVVDRFQQLMNPG
CEEECCCCCEEECCCCEEEEEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCC
FRISGFIESYTGISNAMLADAPSCEEVMAQFADFIGDYNLVAHNASFDQRFLDAELKRIN
CEEHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHH
CHYLGQFACSMLIARRLYQAAPNHKLGSLVSYKNIQHDGVFHRALADSEMTASLWLAMLE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHH
DLQQDYQLHSPDFGLLQSIAKQTKANINQYLQRQG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]