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The map label for this gene is bcr [H]

Identifier: 157961735

GI number: 157961735

Start: 2323131

End: 2324357

Strand: Reverse

Name: bcr [H]

Synonym: Spea_1912

Alternate gene names: 157961735

Gene position: 2324357-2323131 (Counterclockwise)

Preceding gene: 157961736

Following gene: 157961734

Centisome position: 44.92

GC content: 46.05

Gene sequence:

>1227_bases
TTGAACAACTCATCCAATAGTCAAGCAATTAACAAGCAAGCATTTGGCGTCAGTTTAGCTTTGCTGATCCCCATGCTATC
TGCCATTGTCGCTATCACACCGCTTGCTATTGATATGTACCTGCCAGCTATGGCGACGCTAGCAGCCAGTTTCAACACAG
ATATCACCTTAGTACAGCAATCACTCAGTCTTTACTTAGGCGGTTATGCATTAGGTATGCTCTGCTTCGGTCCCTTAGCC
GATAGGTTTGGCCGCAAGCGATTAGTACTTATGGGCTTAACTGGATTTATGCTCTGTAGTCTCGCTCTCGCCTTTGTCAC
CACTATTGAAGCATTCTTAAGCATCCGCTTTTTACAGGCGTTTATCGGTGCTGCGGCAACGGTTGTGGTGCCTGGCTATA
TCAAAGAGCTTTACGGAAAGAACACGGCTAAAGGCATGTCTTATGTCAGCCTGATCATGATGCTCGCCCCGCTAATTGCC
CCTAGTATCGGCAGCCTAATTTTAGAGCTCGGTGACTGGCATCTTATCTTCTTTATCCTAGCTTTCTACGCATTCATTTT
ATTGCTGCTAGTGGGTCTTAAACTTAAGATGCCTAGCGATATAGACAAGAGCAGCCGAAGTACTCAGTCATTTTTTGGCG
CTTATGCGACGGTATTTACAAAAAAAGGCGTAAAGCTCAATATCGCCAGCGGCGTACTCACCTCATTTGCCTTTTTCTGT
TATCTAACGGCTTCGCCATTCGTTTATATGGAAGTGTTTGGCTTAGACAAATCACTATTTGCAATATTGTTTAGTACCAA
TGTTGGCGCCTTAATGTTGGCCAACGTAGTCAACAGTAAGATCGTTGGTCGCTACGGCTCGAAGCGAATGCTTAAAGTTT
CAACCTTCTTCGGAGTAATCGCGGGTATCGCACTGCTGAGCGTTAATCTACTCGGATTGAGCTACCACTTTACCGTGATC
ATGCTATTGCCGCTGATGGCCTGTCTAGGCGTTATGTCAGTCAATGCCGATGCCATTGTACTGATGAAGTTTCAAAAAGA
GACGGGAACCGCTACCGCAGTCATAGGCACATTACGTTTTGGTTTTGGTGCATTAGCGGGCCCCTTGTTGGCACTGTTTT
ATACAGGAACTGCAGTACCCTTTTCGGCTTTAATGCTTTCAGCTGTACTGTTAGTCGGTTTGTGCCAGTTTTTAAGTCGC
CATGAGCCCGCGGCGCTAGAGCAATAG

Upstream 100 bases:

>100_bases
CTCTGTTACCTTATTAAATCGATAATTCGGTAGCTCTACTATGGCGTAAACTAAAGGTTCGATTCAATTCGTAGATTAGT
CATATTTTATTGAGGTATTT

Downstream 100 bases:

>100_bases
AAAGTACAAAGCTTAGCTCTTCAATCTAAAGCCACTCTTTCACCTGCTTATTTGTCATAAAGTTGTTGTACATTAGCTAA
TTCCTCACTGTATTTACTCA

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNNSSNSQAINKQAFGVSLALLIPMLSAIVAITPLAIDMYLPAMATLAASFNTDITLVQQSLSLYLGGYALGMLCFGPLA
DRFGRKRLVLMGLTGFMLCSLALAFVTTIEAFLSIRFLQAFIGAAATVVVPGYIKELYGKNTAKGMSYVSLIMMLAPLIA
PSIGSLILELGDWHLIFFILAFYAFILLLLVGLKLKMPSDIDKSSRSTQSFFGAYATVFTKKGVKLNIASGVLTSFAFFC
YLTASPFVYMEVFGLDKSLFAILFSTNVGALMLANVVNSKIVGRYGSKRMLKVSTFFGVIAGIALLSVNLLGLSYHFTVI
MLLPLMACLGVMSVNADAIVLMKFQKETGTATAVIGTLRFGFGALAGPLLALFYTGTAVPFSALMLSAVLLVGLCQFLSR
HEPAALEQ

Sequences:

>Translated_408_residues
MNNSSNSQAINKQAFGVSLALLIPMLSAIVAITPLAIDMYLPAMATLAASFNTDITLVQQSLSLYLGGYALGMLCFGPLA
DRFGRKRLVLMGLTGFMLCSLALAFVTTIEAFLSIRFLQAFIGAAATVVVPGYIKELYGKNTAKGMSYVSLIMMLAPLIA
PSIGSLILELGDWHLIFFILAFYAFILLLLVGLKLKMPSDIDKSSRSTQSFFGAYATVFTKKGVKLNIASGVLTSFAFFC
YLTASPFVYMEVFGLDKSLFAILFSTNVGALMLANVVNSKIVGRYGSKRMLKVSTFFGVIAGIALLSVNLLGLSYHFTVI
MLLPLMACLGVMSVNADAIVLMKFQKETGTATAVIGTLRFGFGALAGPLLALFYTGTAVPFSALMLSAVLLVGLCQFLSR
HEPAALEQ
>Mature_408_residues
MNNSSNSQAINKQAFGVSLALLIPMLSAIVAITPLAIDMYLPAMATLAASFNTDITLVQQSLSLYLGGYALGMLCFGPLA
DRFGRKRLVLMGLTGFMLCSLALAFVTTIEAFLSIRFLQAFIGAAATVVVPGYIKELYGKNTAKGMSYVSLIMMLAPLIA
PSIGSLILELGDWHLIFFILAFYAFILLLLVGLKLKMPSDIDKSSRSTQSFFGAYATVFTKKGVKLNIASGVLTSFAFFC
YLTASPFVYMEVFGLDKSLFAILFSTNVGALMLANVVNSKIVGRYGSKRMLKVSTFFGVIAGIALLSVNLLGLSYHFTVI
MLLPLMACLGVMSVNADAIVLMKFQKETGTATAVIGTLRFGFGALAGPLLALFYTGTAVPFSALMLSAVLLVGLCQFLSR
HEPAALEQ

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=352, Percent_Identity=35.2272727272727, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI48994889, Length=380, Percent_Identity=29.2105263157895, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790794, Length=265, Percent_Identity=29.0566037735849, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790146, Length=284, Percent_Identity=27.112676056338, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787065, Length=287, Percent_Identity=26.8292682926829, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082312, Length=198, Percent_Identity=26.2626262626263, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319657, Length=161, Percent_Identity=27.9503105590062, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43683; Mature: 43683

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSSNSQAINKQAFGVSLALLIPMLSAIVAITPLAIDMYLPAMATLAASFNTDITLVQQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SLSLYLGGYALGMLCFGPLADRFGRKRLVLMGLTGFMLCSLALAFVTTIEAFLSIRFLQA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGAAATVVVPGYIKELYGKNTAKGMSYVSLIMMLAPLIAPSIGSLILELGDWHLIFFIL
HHCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AFYAFILLLLVGLKLKMPSDIDKSSRSTQSFFGAYATVFTKKGVKLNIASGVLTSFAFFC
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
YLTASPFVYMEVFGLDKSLFAILFSTNVGALMLANVVNSKIVGRYGSKRMLKVSTFFGVI
HHHCCCCEEHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AGIALLSVNLLGLSYHFTVIMLLPLMACLGVMSVNADAIVLMKFQKETGTATAVIGTLRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHH
GFGALAGPLLALFYTGTAVPFSALMLSAVLLVGLCQFLSRHEPAALEQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNNSSNSQAINKQAFGVSLALLIPMLSAIVAITPLAIDMYLPAMATLAASFNTDITLVQQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SLSLYLGGYALGMLCFGPLADRFGRKRLVLMGLTGFMLCSLALAFVTTIEAFLSIRFLQA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGAAATVVVPGYIKELYGKNTAKGMSYVSLIMMLAPLIAPSIGSLILELGDWHLIFFIL
HHCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AFYAFILLLLVGLKLKMPSDIDKSSRSTQSFFGAYATVFTKKGVKLNIASGVLTSFAFFC
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
YLTASPFVYMEVFGLDKSLFAILFSTNVGALMLANVVNSKIVGRYGSKRMLKVSTFFGVI
HHHCCCCEEHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AGIALLSVNLLGLSYHFTVIMLLPLMACLGVMSVNADAIVLMKFQKETGTATAVIGTLRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHH
GFGALAGPLLALFYTGTAVPFSALMLSAVLLVGLCQFLSRHEPAALEQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]