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The map label for this gene is ilvI [H]

Identifier: 157961734

GI number: 157961734

Start: 2321049

End: 2322770

Strand: Reverse

Name: ilvI [H]

Synonym: Spea_1911

Alternate gene names: 157961734

Gene position: 2322770-2321049 (Counterclockwise)

Preceding gene: 157961735

Following gene: 157961733

Centisome position: 44.89

GC content: 46.4

Gene sequence:

>1722_bases
ATGGAGAAGTTATCTGGCGCCAGCATGGTTGTCCGTTCTCTTATCGATGAAGGTGTTAAACATATTTTTGGCTATCCAGG
CGGTTCCGTACTGGATATCTATGATGCCCTACACGAAAAATCAAAGATCGAGCATATCCTAGTCCGTCACGAACAAGCCG
CCGTTCACATGGCCGATGGCTACGCTCGTGCGACAGGTGAAGTAGGTGTTGTACTGGTTACCTCAGGTCCCGGTGCAACT
AATACCATCACAGGTATCGCTACTGCCTATATGGACTCGATTCCTTTGGTGGTTATTTCAGGGCAGGTTCCAAGCAATCT
AATTGGTAATGATGCATTCCAAGAATGCGACATGATCGGTATTTCTAGACCCGTCGTTAAGCACAGCTTCTTAGTGACAG
ATCCTAGAGATATTCCAGCCATCATCAAGAAAGCCTTCTATATCGCCTCGAGTGGCCGACCAGGGCCAGTTGTTGTCGAT
GTGCCAAAAGACTGTATGAACCCAGCGATATTACACGATTACCAATATCCTGAAGATATCAGCATGCGCTCATATAACCC
GACGACTTCAGGCCATAAGGGCCAAGTTCGTCGCGGTCTACAGGCACTGTTACAAGCAAAGAAGCCGGTACTTTATGTCG
GTGGTGGCGCGGTTATATCAGGCTGCGACGCACAAATTTTAGCGCTATCTGAAAAGCTTGGGATCCCTGTTGTCAGTACA
CTAATGGGCTTAGGCGCCTTTCCTGGCACCCACAAAAACAGTGTTGGCATGTTAGGCATGCATGGCTGCTACGAAGCCAA
CATGACCATGCATAATAGTGACCTTATTTTCGGCATTGGCGTACGATTTGATGATCGTACTACCAATAATGTCGACAAAT
ACTGCCCTAACGCTAAAATCTTGCATATTGATATCGACCCTTCTTCTATCTCAAAGACGATTCGAGTCGATATTCCAATT
GTTGGCTCTGCAGAAAAAATCTTAGACGATATGCTTAAGCAGATTGAAGCAAACGATTATGGCATCACAGATCCTGCTGC
AAATGATGAGTGGTGGAGTGATATTGCCCAGTGGCGCGCCGTTGATAGCCTAAAATACTCCACTAATGACAAGAAAATTA
AACCACAACAGGTCATAGAAGCCATACATAAACTCACCAATGGTGATGCCTACGTGGCATCCGATGTGGGTCAGCACCAG
ATGTTCGCTGCACTCTATTACCCGTTCAATAAACCACGCCGTTGGATCAACTCTGGTGGTCTAGGCACCATGGGCTTTGG
ATTACCTGCAGCGTTAGGCGTCAAGCTTGCAAAACCCGATGAGACGGTCATCTGTGTAACCGGTGATGGCTCAATTCAAA
TGAATATTCAGGAGCTTTCAACGGCGCTGCAGTACGATATTCCTGTGATTATTGTCAACTTGAATAATCATTTCTTAGGA
ATGGTAAAGCAGTGGCAAGACATGATTTATGCCGGCCGTCACTCCCAGTCTTATATGGATTCAGTACCTGATTTTGCCAA
GATTTCTGAAGCTTATGGCCATGTAGGCATGACCATTAGCAACCCAGCAGAATTAGAGGCGGGGCTTAAAAAAGCACTTA
GCATGAAAGACAAGCTGGTGTTTATGGATATTCACGTCGATGAGACCGAGCACGTTCACCCGATGCAGATCCGCGGTGGT
GCGATGAATGAGATGTGGCTAAGCAAAACGGAGAAAAGCTAA

Upstream 100 bases:

>100_bases
TTCGCGAGCAAAGATTACCGTCTGAGCTTAATTATTAGAGCGATTACCGTCGCTGTATCAAGATACTGCATACCGTCGCG
GTAACTTATGAGGAAGTACT

Downstream 100 bases:

>100_bases
TGCGTCGTATTATATGTGTATTACTTGAAAACCAACCCGGCGCCCTTTCACGAGTTGTCGGTCTGTTTTCACAGCGCGGT
TATAATATCGAGACTTTAAC

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MEKLSGASMVVRSLIDEGVKHIFGYPGGSVLDIYDALHEKSKIEHILVRHEQAAVHMADGYARATGEVGVVLVTSGPGAT
NTITGIATAYMDSIPLVVISGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVTDPRDIPAIIKKAFYIASSGRPGPVVVD
VPKDCMNPAILHDYQYPEDISMRSYNPTTSGHKGQVRRGLQALLQAKKPVLYVGGGAVISGCDAQILALSEKLGIPVVST
LMGLGAFPGTHKNSVGMLGMHGCYEANMTMHNSDLIFGIGVRFDDRTTNNVDKYCPNAKILHIDIDPSSISKTIRVDIPI
VGSAEKILDDMLKQIEANDYGITDPAANDEWWSDIAQWRAVDSLKYSTNDKKIKPQQVIEAIHKLTNGDAYVASDVGQHQ
MFAALYYPFNKPRRWINSGGLGTMGFGLPAALGVKLAKPDETVICVTGDGSIQMNIQELSTALQYDIPVIIVNLNNHFLG
MVKQWQDMIYAGRHSQSYMDSVPDFAKISEAYGHVGMTISNPAELEAGLKKALSMKDKLVFMDIHVDETEHVHPMQIRGG
AMNEMWLSKTEKS

Sequences:

>Translated_573_residues
MEKLSGASMVVRSLIDEGVKHIFGYPGGSVLDIYDALHEKSKIEHILVRHEQAAVHMADGYARATGEVGVVLVTSGPGAT
NTITGIATAYMDSIPLVVISGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVTDPRDIPAIIKKAFYIASSGRPGPVVVD
VPKDCMNPAILHDYQYPEDISMRSYNPTTSGHKGQVRRGLQALLQAKKPVLYVGGGAVISGCDAQILALSEKLGIPVVST
LMGLGAFPGTHKNSVGMLGMHGCYEANMTMHNSDLIFGIGVRFDDRTTNNVDKYCPNAKILHIDIDPSSISKTIRVDIPI
VGSAEKILDDMLKQIEANDYGITDPAANDEWWSDIAQWRAVDSLKYSTNDKKIKPQQVIEAIHKLTNGDAYVASDVGQHQ
MFAALYYPFNKPRRWINSGGLGTMGFGLPAALGVKLAKPDETVICVTGDGSIQMNIQELSTALQYDIPVIIVNLNNHFLG
MVKQWQDMIYAGRHSQSYMDSVPDFAKISEAYGHVGMTISNPAELEAGLKKALSMKDKLVFMDIHVDETEHVHPMQIRGG
AMNEMWLSKTEKS
>Mature_573_residues
MEKLSGASMVVRSLIDEGVKHIFGYPGGSVLDIYDALHEKSKIEHILVRHEQAAVHMADGYARATGEVGVVLVTSGPGAT
NTITGIATAYMDSIPLVVISGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVTDPRDIPAIIKKAFYIASSGRPGPVVVD
VPKDCMNPAILHDYQYPEDISMRSYNPTTSGHKGQVRRGLQALLQAKKPVLYVGGGAVISGCDAQILALSEKLGIPVVST
LMGLGAFPGTHKNSVGMLGMHGCYEANMTMHNSDLIFGIGVRFDDRTTNNVDKYCPNAKILHIDIDPSSISKTIRVDIPI
VGSAEKILDDMLKQIEANDYGITDPAANDEWWSDIAQWRAVDSLKYSTNDKKIKPQQVIEAIHKLTNGDAYVASDVGQHQ
MFAALYYPFNKPRRWINSGGLGTMGFGLPAALGVKLAKPDETVICVTGDGSIQMNIQELSTALQYDIPVIIVNLNNHFLG
MVKQWQDMIYAGRHSQSYMDSVPDFAKISEAYGHVGMTISNPAELEAGLKKALSMKDKLVFMDIHVDETEHVHPMQIRGG
AMNEMWLSKTEKS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=593, Percent_Identity=25.9696458684654, Blast_Score=186, Evalue=7e-47,
Organism=Homo sapiens, GI21361361, Length=513, Percent_Identity=26.7056530214425, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=71.304347826087, Blast_Score=879, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=39.2920353982301, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1786717, Length=546, Percent_Identity=34.0659340659341, Blast_Score=324, Evalue=9e-90,
Organism=Escherichia coli, GI1787096, Length=561, Percent_Identity=29.5900178253119, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788716, Length=578, Percent_Identity=23.8754325259516, Blast_Score=162, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=552, Percent_Identity=26.6304347826087, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=586, Percent_Identity=25.5972696245734, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=518, Percent_Identity=27.6061776061776, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=40.893470790378, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320816, Length=474, Percent_Identity=23.6286919831224, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323163, Length=496, Percent_Identity=23.5887096774194, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=510, Percent_Identity=21.7647058823529, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=499, Percent_Identity=23.4468937875752, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320588, Length=330, Percent_Identity=24.2424242424242, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI19922626, Length=596, Percent_Identity=23.3221476510067, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62422; Mature: 62422

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSGASMVVRSLIDEGVKHIFGYPGGSVLDIYDALHEKSKIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEECCC
YARATGEVGVVLVTSGPGATNTITGIATAYMDSIPLVVISGQVPSNLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCC
ISRPVVKHSFLVTDPRDIPAIIKKAFYIASSGRPGPVVVDVPKDCMNPAILHDYQYPEDI
CCCCHHHCEEEEECCCHHHHHHHHHHEEECCCCCCCEEEECCHHHCCCCEEECCCCCCCC
SMRSYNPTTSGHKGQVRRGLQALLQAKKPVLYVGGGAVISGCDAQILALSEKLGIPVVST
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEEEEHHHCCCHHHHH
LMGLGAFPGTHKNSVGMLGMHGCYEANMTMHNSDLIFGIGVRFDDRTTNNVDKYCPNAKI
HHHCCCCCCCCCCCCCEEECCCEEECCEEEECCCEEEEEEEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTIRVDIPIVGSAEKILDDMLKQIEANDYGITDPAANDEWWSDIAQWRA
EEEECCHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VDSLKYSTNDKKIKPQQVIEAIHKLTNGDAYVASDVGQHQMFAALYYPFNKPRRWINSGG
HHCEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEECCCCCCHHHHCCCC
LGTMGFGLPAALGVKLAKPDETVICVTGDGSIQMNIQELSTALQYDIPVIIVNLNNHFLG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCHHHH
MVKQWQDMIYAGRHSQSYMDSVPDFAKISEAYGHVGMTISNPAELEAGLKKALSMKDKLV
HHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCEE
FMDIHVDETEHVHPMQIRGGAMNEMWLSKTEKS
EEEEEECCCCCCCCEEECCCCCHHHHHHCCCCC
>Mature Secondary Structure
MEKLSGASMVVRSLIDEGVKHIFGYPGGSVLDIYDALHEKSKIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEECCC
YARATGEVGVVLVTSGPGATNTITGIATAYMDSIPLVVISGQVPSNLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCC
ISRPVVKHSFLVTDPRDIPAIIKKAFYIASSGRPGPVVVDVPKDCMNPAILHDYQYPEDI
CCCCHHHCEEEEECCCHHHHHHHHHHEEECCCCCCCEEEECCHHHCCCCEEECCCCCCCC
SMRSYNPTTSGHKGQVRRGLQALLQAKKPVLYVGGGAVISGCDAQILALSEKLGIPVVST
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEEEEHHHCCCHHHHH
LMGLGAFPGTHKNSVGMLGMHGCYEANMTMHNSDLIFGIGVRFDDRTTNNVDKYCPNAKI
HHHCCCCCCCCCCCCCEEECCCEEECCEEEECCCEEEEEEEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTIRVDIPIVGSAEKILDDMLKQIEANDYGITDPAANDEWWSDIAQWRA
EEEECCHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VDSLKYSTNDKKIKPQQVIEAIHKLTNGDAYVASDVGQHQMFAALYYPFNKPRRWINSGG
HHCEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEECCCCCCHHHHCCCC
LGTMGFGLPAALGVKLAKPDETVICVTGDGSIQMNIQELSTALQYDIPVIIVNLNNHFLG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCHHHH
MVKQWQDMIYAGRHSQSYMDSVPDFAKISEAYGHVGMTISNPAELEAGLKKALSMKDKLV
HHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCEE
FMDIHVDETEHVHPMQIRGGAMNEMWLSKTEKS
EEEEEECCCCCCCCEEECCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]