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The map label for this gene is ushA [H]

Identifier: 157961731

GI number: 157961731

Start: 2318021

End: 2319739

Strand: Direct

Name: ushA [H]

Synonym: Spea_1908

Alternate gene names: 157961731

Gene position: 2318021-2319739 (Clockwise)

Preceding gene: 157961730

Following gene: 157961732

Centisome position: 44.8

GC content: 42.99

Gene sequence:

>1719_bases
ATGAATAAGATTTTACTAAAAGGACTCGTCACTACGGCTGTTGTCACTGCATTAACGGCTTGTGGTAGCGATAATAATGA
TAGTGATGGTTCTATAAGCACATTACCTATGACATGTGTTGAAGCGGGTGATGCTTGTACTAAGTTTACTGTATTACACA
CTAATGATAACCATGGCCGTTTTTTTGAAAACAAATATGGCGAATACGGCATGGCAGCTCGTAAGACACTGATCGAACAG
ATCCGTGCTGAAGTTGAAGCAAATGGTGGCGAGTCAATCTTACTGTCTGGCGGTGATGTTAACACTGGCGTTCCAGAATC
TGATTTACAAGATGCGCGTCCAGATTTTATTGGTATGAATTTGATTGGTTATGATGCTATGGCTGTCGGTAATCATGAAT
TTGATAATCCCTTATCAATAGTTAATATGCAGCGCGAACTTGCCGAATTCCCAATGCTAGCTGCAAACATATATGACAAG
GTATCCGGTGAGCGCTATTTTGATGCCTATAAAGTGTTTGATGTTAATGGGATCCGTATTGCAGTTGTCGGTTTAACAAC
TGAAGATACAGCTAAGATTGCTAACCCAGAGTTTGTAAGTGGGCTTGAGTTTACCGATCCAAAAGAAGAGTTGAAGAAGG
TAATTAAAGAGATCAATGATGCTGATAAAGCTGACTTGATTTTCGCAACGACCCATATGGGGCACTACGCTGATGGTGCT
AACGGAAATAATGCGCCAGGTGATGTTGCACTTGCTCGTGCAATGGCAGCGGGAGAGTTAGCAGCCGTCATTGGTGGACA
CTCGCAAAACCCTGTATGTATGGAAGGGAATGAATACGATTCAGAGTTTACTCCCGGTGGTGAATGTAAGCCAGATGTTC
AGAACGGTACATTTATCATGCAAGCTCATGAGTGGGGTAAGTATGTTGGTCAAGCGAACTTTGAATACTTCAATGATAAT
CTACACCTAGCGAGTTACAAGCTGATCCCTGTAAACCTAAAAGAGAAGAACGAAGCGGGCGAACGTGTATTAATCGGTGA
ACCTATCAAACCAGATGCAACTGTGCTGGAAATTTTGCAGCAGTATCAAGATAAAGGGCAGGAAGGACTATCTGAAGTTA
TCGGTACAACAGATGGAAAACTTGAAGGAGATCGTGGTGTTGTACGTACTCAGCAAACAAATTTAGGTCGACTGATTGCT
GAAGCACAGCGTAGTAAAGTGGATGCTGACTTTGCGGTAATGAATTCAGGTGGGGTACGTGATTCTATCGAAGCCGGTGA
GATCACCTATAAAGATGTTCTTACTGTTCAGCCATTTGGCAACTCCATTACTAAGAGTACATTGAGTGGGGCTGAAATTA
CAGACTACCTGAACGTCGTTGCGACTGTAGATATCGGTGTTGAGGGCTCTGGTGCATACCCACAGCTTGCGGGTATTAAG
ATGGAAGTTGATTGTGAAGCGACAACAGTTGTTATCAACGACATAAATGGTAAAGGTTATGATGCTGACACAATGTACAG
CTTTACTGTTCCTAGTTACAACGCCGCTGGCGGCGATGGCTACCCAGTACTGACTCCTGTAATGACAGGATATGTGGACG
CAGAAGTAATGTATACCTTCATCAAAGACATTGGTGATATCAAGATCGCAGAATTCGCACCAGTAGCAGGTGATGTGCAA
TTCCTAAACAGTAAGAGCACTATGGCTTGTCAGCTATAA

Upstream 100 bases:

>100_bases
GTATATGATTTGCTAAGATGGGGCGCGGCTAAAGCAAGTTATGCTTTTGCTAGATAACGAAAAGTTACAGCATATTAAAA
TAATCAAGGGGTTGATTGTA

Downstream 100 bases:

>100_bases
TTAACTAAGTTAATCCGGAAAAGCAGCCTTGTGGCTGCTTTTTTTATATTTTTCACATACAGCGAATTTAAGGCTTAAGC
TCATTCAAAAATTTTTTTCA

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MNKILLKGLVTTAVVTALTACGSDNNDSDGSISTLPMTCVEAGDACTKFTVLHTNDNHGRFFENKYGEYGMAARKTLIEQ
IRAEVEANGGESILLSGGDVNTGVPESDLQDARPDFIGMNLIGYDAMAVGNHEFDNPLSIVNMQRELAEFPMLAANIYDK
VSGERYFDAYKVFDVNGIRIAVVGLTTEDTAKIANPEFVSGLEFTDPKEELKKVIKEINDADKADLIFATTHMGHYADGA
NGNNAPGDVALARAMAAGELAAVIGGHSQNPVCMEGNEYDSEFTPGGECKPDVQNGTFIMQAHEWGKYVGQANFEYFNDN
LHLASYKLIPVNLKEKNEAGERVLIGEPIKPDATVLEILQQYQDKGQEGLSEVIGTTDGKLEGDRGVVRTQQTNLGRLIA
EAQRSKVDADFAVMNSGGVRDSIEAGEITYKDVLTVQPFGNSITKSTLSGAEITDYLNVVATVDIGVEGSGAYPQLAGIK
MEVDCEATTVVINDINGKGYDADTMYSFTVPSYNAAGGDGYPVLTPVMTGYVDAEVMYTFIKDIGDIKIAEFAPVAGDVQ
FLNSKSTMACQL

Sequences:

>Translated_572_residues
MNKILLKGLVTTAVVTALTACGSDNNDSDGSISTLPMTCVEAGDACTKFTVLHTNDNHGRFFENKYGEYGMAARKTLIEQ
IRAEVEANGGESILLSGGDVNTGVPESDLQDARPDFIGMNLIGYDAMAVGNHEFDNPLSIVNMQRELAEFPMLAANIYDK
VSGERYFDAYKVFDVNGIRIAVVGLTTEDTAKIANPEFVSGLEFTDPKEELKKVIKEINDADKADLIFATTHMGHYADGA
NGNNAPGDVALARAMAAGELAAVIGGHSQNPVCMEGNEYDSEFTPGGECKPDVQNGTFIMQAHEWGKYVGQANFEYFNDN
LHLASYKLIPVNLKEKNEAGERVLIGEPIKPDATVLEILQQYQDKGQEGLSEVIGTTDGKLEGDRGVVRTQQTNLGRLIA
EAQRSKVDADFAVMNSGGVRDSIEAGEITYKDVLTVQPFGNSITKSTLSGAEITDYLNVVATVDIGVEGSGAYPQLAGIK
MEVDCEATTVVINDINGKGYDADTMYSFTVPSYNAAGGDGYPVLTPVMTGYVDAEVMYTFIKDIGDIKIAEFAPVAGDVQ
FLNSKSTMACQL
>Mature_572_residues
MNKILLKGLVTTAVVTALTACGSDNNDSDGSISTLPMTCVEAGDACTKFTVLHTNDNHGRFFENKYGEYGMAARKTLIEQ
IRAEVEANGGESILLSGGDVNTGVPESDLQDARPDFIGMNLIGYDAMAVGNHEFDNPLSIVNMQRELAEFPMLAANIYDK
VSGERYFDAYKVFDVNGIRIAVVGLTTEDTAKIANPEFVSGLEFTDPKEELKKVIKEINDADKADLIFATTHMGHYADGA
NGNNAPGDVALARAMAAGELAAVIGGHSQNPVCMEGNEYDSEFTPGGECKPDVQNGTFIMQAHEWGKYVGQANFEYFNDN
LHLASYKLIPVNLKEKNEAGERVLIGEPIKPDATVLEILQQYQDKGQEGLSEVIGTTDGKLEGDRGVVRTQQTNLGRLIA
EAQRSKVDADFAVMNSGGVRDSIEAGEITYKDVLTVQPFGNSITKSTLSGAEITDYLNVVATVDIGVEGSGAYPQLAGIK
MEVDCEATTVVINDINGKGYDADTMYSFTVPSYNAAGGDGYPVLTPVMTGYVDAEVMYTFIKDIGDIKIAEFAPVAGDVQ
FLNSKSTMACQL

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=563, Percent_Identity=25.2220248667851, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786687, Length=529, Percent_Identity=51.9848771266541, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1790658, Length=569, Percent_Identity=20.3866432337434, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922446, Length=581, Percent_Identity=24.9569707401033, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24654424, Length=581, Percent_Identity=24.9569707401033, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19922444, Length=544, Percent_Identity=24.2647058823529, Blast_Score=120, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24641187, Length=560, Percent_Identity=24.1071428571429, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28573524, Length=529, Percent_Identity=24.952741020794, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI221329836, Length=518, Percent_Identity=22.972972972973, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI161076508, Length=432, Percent_Identity=24.537037037037, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19921980, Length=432, Percent_Identity=24.3055555555556, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24652512, Length=432, Percent_Identity=24.537037037037, Blast_Score=86, Evalue=9e-17,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 61446; Mature: 61446

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKILLKGLVTTAVVTALTACGSDNNDSDGSISTLPMTCVEAGDACTKFTVLHTNDNHGR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCEEEEEEEEECCCCCC
FFENKYGEYGMAARKTLIEQIRAEVEANGGESILLSGGDVNTGVPESDLQDARPDFIGMN
CHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEEE
LIGYDAMAVGNHEFDNPLSIVNMQRELAEFPMLAANIYDKVSGERYFDAYKVFDVNGIRI
EECCCEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECEEEEEECCCEEE
AVVGLTTEDTAKIANPEFVSGLEFTDPKEELKKVIKEINDADKADLIFATTHMGHYADGA
EEEEECCCCCHHCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
NGNNAPGDVALARAMAAGELAAVIGGHSQNPVCMEGNEYDSEFTPGGECKPDVQNGTFIM
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEE
QAHEWGKYVGQANFEYFNDNLHLASYKLIPVNLKEKNEAGERVLIGEPIKPDATVLEILQ
EECHHHHHHCCCCCEEECCCEEEEEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHH
QYQDKGQEGLSEVIGTTDGKLEGDRGVVRTQQTNLGRLIAEAQRSKVDADFAVMNSGGVR
HHHHCCHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCC
DSIEAGEITYKDVLTVQPFGNSITKSTLSGAEITDYLNVVATVDIGVEGSGAYPQLAGIK
CCCCCCCEEEEHEEEEECCCCCHHHHHCCCCHHHHHEEEEEEEEECCCCCCCCCCCCCEE
MEVDCEATTVVINDINGKGYDADTMYSFTVPSYNAAGGDGYPVLTPVMTGYVDAEVMYTF
EEECCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHHHH
IKDIGDIKIAEFAPVAGDVQFLNSKSTMACQL
HHHCCCEEEEEECCCCCCEEEECCCCCEEECC
>Mature Secondary Structure
MNKILLKGLVTTAVVTALTACGSDNNDSDGSISTLPMTCVEAGDACTKFTVLHTNDNHGR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCEEEEEEEEECCCCCC
FFENKYGEYGMAARKTLIEQIRAEVEANGGESILLSGGDVNTGVPESDLQDARPDFIGMN
CHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEEE
LIGYDAMAVGNHEFDNPLSIVNMQRELAEFPMLAANIYDKVSGERYFDAYKVFDVNGIRI
EECCCEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECEEEEEECCCEEE
AVVGLTTEDTAKIANPEFVSGLEFTDPKEELKKVIKEINDADKADLIFATTHMGHYADGA
EEEEECCCCCHHCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
NGNNAPGDVALARAMAAGELAAVIGGHSQNPVCMEGNEYDSEFTPGGECKPDVQNGTFIM
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEE
QAHEWGKYVGQANFEYFNDNLHLASYKLIPVNLKEKNEAGERVLIGEPIKPDATVLEILQ
EECHHHHHHCCCCCEEECCCEEEEEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHH
QYQDKGQEGLSEVIGTTDGKLEGDRGVVRTQQTNLGRLIAEAQRSKVDADFAVMNSGGVR
HHHHCCHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCC
DSIEAGEITYKDVLTVQPFGNSITKSTLSGAEITDYLNVVATVDIGVEGSGAYPQLAGIK
CCCCCCCEEEEHEEEEECCCCCHHHHHCCCCHHHHHEEEEEEEEECCCCCCCCCCCCCEE
MEVDCEATTVVINDINGKGYDADTMYSFTVPSYNAAGGDGYPVLTPVMTGYVDAEVMYTF
EEECCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHHHH
IKDIGDIKIAEFAPVAGDVQFLNSKSTMACQL
HHHCCCEEEEEECCCCCCEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA