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The map label for this gene is estA [H]

Identifier: 157961730

GI number: 157961730

Start: 2316970

End: 2317650

Strand: Direct

Name: estA [H]

Synonym: Spea_1907

Alternate gene names: 157961730

Gene position: 2316970-2317650 (Clockwise)

Preceding gene: 157961729

Following gene: 157961731

Centisome position: 44.78

GC content: 46.84

Gene sequence:

>681_bases
ATGTCTGCCATTCAATTAGAACGTATTACCATTGAGCCCACTCAAGAGGCTAAAGCCTGTGTTATTTGGCTTCATGGTCT
AGGTGACTCCGGCGCAGGCTTTGCTCCTGTAGTGCCAGCATTAGGCTTAGGTTCAGATCATGGCATCCGCTTTATATTCC
CTCATGCGCCAGAGCAAGCGGTGACCATTAATGGTGGCTATGTCATGCGTGCTTGGTACGACATCAAGAGTATGGATCTA
CATGATCGAGCAGACAAAAAAGGTGTAGAGCAGAGCGAGAAGCAGATCATCGCACTGATTGAAGAGCAAGTAGCGCTTGG
GATCCCAACAGAAAATATCGTATTAGCAGGTTTTAGCCAAGGTGGTGTGATGAGCCTCTATACTGGGCTTAGACTGCCAT
ACAAACTTGCAGGGATCATGGCGCTGTCTTGTTACCTTCCAAGCGGTGACTCATTACCTAATGGGCTTTCAGATGTGAAT
CGTGACACGCCGATTTTACAGCATCACGGCATTGATGATGATGTAGTTCCGGTTGATGCAGGTAAAATGGCTTACGAGTT
ACTGCAAGGCGCAGGGTTTAATACCCAATGGAAAACTTACAATATGCCTCACAGTGTTTTGCCTCAGCAGCTACAAGATA
TCTCTGCTTGGTTACAGCAAGTACTGGCAAACGGTAAGTAG

Upstream 100 bases:

>100_bases
ATCCTAAACATTGCAGCTAGTACTGCTTTCTCATCATGTGACAAATTTCAATTGGCTATTATTTCAGCTTTTGTGACAAT
ACTAAATATAAAGGAACTTT

Downstream 100 bases:

>100_bases
AGACTGTTTGCTTGTTTTTTGAACAGGCAAGGGCATTGATTTTTTGCCTCGCTTGTTCTCAATTGAGGATAATCCGTTAG
CTATCGTTACAGATATAAGC

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSAIQLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSDHGIRFIFPHAPEQAVTINGGYVMRAWYDIKSMDL
HDRADKKGVEQSEKQIIALIEEQVALGIPTENIVLAGFSQGGVMSLYTGLRLPYKLAGIMALSCYLPSGDSLPNGLSDVN
RDTPILQHHGIDDDVVPVDAGKMAYELLQGAGFNTQWKTYNMPHSVLPQQLQDISAWLQQVLANGK

Sequences:

>Translated_226_residues
MSAIQLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSDHGIRFIFPHAPEQAVTINGGYVMRAWYDIKSMDL
HDRADKKGVEQSEKQIIALIEEQVALGIPTENIVLAGFSQGGVMSLYTGLRLPYKLAGIMALSCYLPSGDSLPNGLSDVN
RDTPILQHHGIDDDVVPVDAGKMAYELLQGAGFNTQWKTYNMPHSVLPQQLQDISAWLQQVLANGK
>Mature_225_residues
SAIQLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSDHGIRFIFPHAPEQAVTINGGYVMRAWYDIKSMDLH
DRADKKGVEQSEKQIIALIEEQVALGIPTENIVLAGFSQGGVMSLYTGLRLPYKLAGIMALSCYLPSGDSLPNGLSDVNR
DTPILQHHGIDDDVVPVDAGKMAYELLQGAGFNTQWKTYNMPHSVLPQQLQDISAWLQQVLANGK

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=212, Percent_Identity=42.4528301886792, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI9966764, Length=216, Percent_Identity=38.8888888888889, Blast_Score=151, Evalue=4e-37,
Organism=Homo sapiens, GI20270341, Length=218, Percent_Identity=27.0642201834862, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508057, Length=216, Percent_Identity=35.1851851851852, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323147, Length=217, Percent_Identity=35.0230414746544, Blast_Score=129, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45553055, Length=216, Percent_Identity=35.6481481481481, Blast_Score=143, Evalue=9e-35,
Organism=Drosophila melanogaster, GI45553057, Length=216, Percent_Identity=35.6481481481481, Blast_Score=143, Evalue=9e-35,
Organism=Drosophila melanogaster, GI21357257, Length=216, Percent_Identity=35.6481481481481, Blast_Score=143, Evalue=9e-35,
Organism=Drosophila melanogaster, GI161078179, Length=222, Percent_Identity=35.1351351351351, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24503; Mature: 24372

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIQLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSDHGIRFIFPHAPEQA
CCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCE
VTINGGYVMRAWYDIKSMDLHDRADKKGVEQSEKQIIALIEEQVALGIPTENIVLAGFSQ
EEECCCEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GGVMSLYTGLRLPYKLAGIMALSCYLPSGDSLPNGLSDVNRDTPILQHHGIDDDVVPVDA
CCHHHHHHHCCCCHHHHHHHEEEEECCCCCCCCCCHHHCCCCCCCHHHCCCCCCEEECCC
GKMAYELLQGAGFNTQWKTYNMPHSVLPQQLQDISAWLQQVLANGK
HHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAIQLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSDHGIRFIFPHAPEQA
CCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCE
VTINGGYVMRAWYDIKSMDLHDRADKKGVEQSEKQIIALIEEQVALGIPTENIVLAGFSQ
EEECCCEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GGVMSLYTGLRLPYKLAGIMALSCYLPSGDSLPNGLSDVNRDTPILQHHGIDDDVVPVDA
CCHHHHHHHCCCCHHHHHHHEEEEECCCCCCCCCCHHHCCCCCCCHHHCCCCCCEEECCC
GKMAYELLQGAGFNTQWKTYNMPHSVLPQQLQDISAWLQQVLANGK
HHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA