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The map label for this gene is liaS [H]

Identifier: 157961558

GI number: 157961558

Start: 2109241

End: 2110434

Strand: Reverse

Name: liaS [H]

Synonym: Spea_1734

Alternate gene names: 157961558

Gene position: 2110434-2109241 (Counterclockwise)

Preceding gene: 157961569

Following gene: 157961557

Centisome position: 40.78

GC content: 42.96

Gene sequence:

>1194_bases
ATGAAAAATCAAGACGATTACGATTTCGCACAATTTAATCAAACCCTACTCTCTATATCTACCCAGCTTCTGGATACTCC
AACAAGCTCGTTAGCGGAGGTGATGAATTCAGTTCTCGACGAGTGTAGAGAACTGCTTGGTGTCGACAGAATATCTTGTT
TGCCTAACACCAAATATATTGCTCAGGAATGGTCGTTTTATGCCGTTTCAGGAAAAAATATTCCTCATGTAAGGCCCAAC
CTTAGCTACGAAATTAAAGCGCGATATCAAGCGATGATCCATTCCAGTGAAGTTTTCAATAGCGATGATGATCTGCAACT
ACTCGAGCTTGCCTGTCAACTGCAGCAACAAAAGCCACTCAATCATATTTTGATCCCGATCCAAGCGATGGGTAAGCCAT
GGGGGGCCTTGGCTGCGGCAAACTTTGTTACTCATAAGGCGTTTGATGAACACTTTATTCGCTGCTCGACTATTTTGGGG
AATATTATGGCTTCAAGTATTGAGCGGCTACGACATTACCAAGAGTTATCTCATCAAAAAGAGAAAGTTGTCGCATTAAA
TAAACGCCTGATAGAAGACAGAGAGAAACAGCTAAAAGTTATCGCTAGAGATCTACACGATGACTTTGGCCAGCGTCTCG
CTTCACTCAACCTTGAATTGGGATTTATTAATACCCAATTAGATAATGAATCTCAAAAAGCACTGTATGATGTAGCAGCA
AAGCTGTCTGATATTACCCGAGATCTTCAGCATCTATCTCGCCATCTTCATCCCGCGATAATAGACAAGGTCGGTCTGTA
TGCCGCCATTGAGGCTGAAGCAAAAAAAATGACCTTGCATAAGAATATTCTTCTGTCACTCAATCTCAGTAAAGAGATCT
TGTACAGTGAGGGACAAAAGCTACATATCTATCGCATCAGCCAAGAAGCGCTTGCTAATGTGATGCGACACTCCCATGCA
ACGGCATTGTCCATCTGCCTAAATCGTGAGGGCAAGTTTGCTAAACTGACTATTGAAGATAACGGTAAGGGCATGGCTCA
GGATGCGCTTGCAAACTCCTCGTCATTAGGGATTGAGTCTATGCTCGAGCGGGCGGCGCTTATTGGTGGCACAATCGATT
TTACTGTCGGTCATCATCAATGTGGATTTTCGGTAAAACTCGTTTGGCCCATTCCAGAAGATATGATTAACTAA

Upstream 100 bases:

>100_bases
AACTAGGACTATTCCTAAAGCAGTCATTACAGTTAGTATTATTTGTGCTTTTACAGGTATAAAGAAATTCAAAACGGATA
ATTGTGGTGAGAAAATAGCT

Downstream 100 bases:

>100_bases
TAGCAAGCATACTAAATATATAATTGATTTTAATAAGAATGATTTTAATAAGAATGATTTTAATAAGAATGATTTAAATA
AGAGTGATATTAATAAGAGT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKNQDDYDFAQFNQTLLSISTQLLDTPTSSLAEVMNSVLDECRELLGVDRISCLPNTKYIAQEWSFYAVSGKNIPHVRPN
LSYEIKARYQAMIHSSEVFNSDDDLQLLELACQLQQQKPLNHILIPIQAMGKPWGALAAANFVTHKAFDEHFIRCSTILG
NIMASSIERLRHYQELSHQKEKVVALNKRLIEDREKQLKVIARDLHDDFGQRLASLNLELGFINTQLDNESQKALYDVAA
KLSDITRDLQHLSRHLHPAIIDKVGLYAAIEAEAKKMTLHKNILLSLNLSKEILYSEGQKLHIYRISQEALANVMRHSHA
TALSICLNREGKFAKLTIEDNGKGMAQDALANSSSLGIESMLERAALIGGTIDFTVGHHQCGFSVKLVWPIPEDMIN

Sequences:

>Translated_397_residues
MKNQDDYDFAQFNQTLLSISTQLLDTPTSSLAEVMNSVLDECRELLGVDRISCLPNTKYIAQEWSFYAVSGKNIPHVRPN
LSYEIKARYQAMIHSSEVFNSDDDLQLLELACQLQQQKPLNHILIPIQAMGKPWGALAAANFVTHKAFDEHFIRCSTILG
NIMASSIERLRHYQELSHQKEKVVALNKRLIEDREKQLKVIARDLHDDFGQRLASLNLELGFINTQLDNESQKALYDVAA
KLSDITRDLQHLSRHLHPAIIDKVGLYAAIEAEAKKMTLHKNILLSLNLSKEILYSEGQKLHIYRISQEALANVMRHSHA
TALSICLNREGKFAKLTIEDNGKGMAQDALANSSSLGIESMLERAALIGGTIDFTVGHHQCGFSVKLVWPIPEDMIN
>Mature_397_residues
MKNQDDYDFAQFNQTLLSISTQLLDTPTSSLAEVMNSVLDECRELLGVDRISCLPNTKYIAQEWSFYAVSGKNIPHVRPN
LSYEIKARYQAMIHSSEVFNSDDDLQLLELACQLQQQKPLNHILIPIQAMGKPWGALAAANFVTHKAFDEHFIRCSTILG
NIMASSIERLRHYQELSHQKEKVVALNKRLIEDREKQLKVIARDLHDDFGQRLASLNLELGFINTQLDNESQKALYDVAA
KLSDITRDLQHLSRHLHPAIIDKVGLYAAIEAEAKKMTLHKNILLSLNLSKEILYSEGQKLHIYRISQEALANVMRHSHA
TALSICLNREGKFAKLTIEDNGKGMAQDALANSSSLGIESMLERAALIGGTIDFTVGHHQCGFSVKLVWPIPEDMIN

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=228, Percent_Identity=29.3859649122807, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787474, Length=254, Percent_Identity=25.9842519685039, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44709; Mature: 44709

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQDDYDFAQFNQTLLSISTQLLDTPTSSLAEVMNSVLDECRELLGVDRISCLPNTKYI
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHH
AQEWSFYAVSGKNIPHVRPNLSYEIKARYQAMIHSSEVFNSDDDLQLLELACQLQQQKPL
HHCCCEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
NHILIPIQAMGKPWGALAAANFVTHKAFDEHFIRCSTILGNIMASSIERLRHYQELSHQK
CCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKVVALNKRLIEDREKQLKVIARDLHDDFGQRLASLNLELGFINTQLDNESQKALYDVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHH
KLSDITRDLQHLSRHLHPAIIDKVGLYAAIEAEAKKMTLHKNILLSLNLSKEILYSEGQK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHHHHHHHHHEEEEEECCCHHHHHCCCCE
LHIYRISQEALANVMRHSHATALSICLNREGKFAKLTIEDNGKGMAQDALANSSSLGIES
EEEEEECHHHHHHHHHHCCHHHHHHEECCCCCEEEEEEECCCCCHHHHHHCCCCCCCHHH
MLERAALIGGTIDFTVGHHQCGFSVKLVWPIPEDMIN
HHHHHHHHCCCEEEEECCCCCCEEEEEEECCCHHHCC
>Mature Secondary Structure
MKNQDDYDFAQFNQTLLSISTQLLDTPTSSLAEVMNSVLDECRELLGVDRISCLPNTKYI
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHH
AQEWSFYAVSGKNIPHVRPNLSYEIKARYQAMIHSSEVFNSDDDLQLLELACQLQQQKPL
HHCCCEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
NHILIPIQAMGKPWGALAAANFVTHKAFDEHFIRCSTILGNIMASSIERLRHYQELSHQK
CCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKVVALNKRLIEDREKQLKVIARDLHDDFGQRLASLNLELGFINTQLDNESQKALYDVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHH
KLSDITRDLQHLSRHLHPAIIDKVGLYAAIEAEAKKMTLHKNILLSLNLSKEILYSEGQK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHHHHHHHHHEEEEEECCCHHHHHCCCCE
LHIYRISQEALANVMRHSHATALSICLNREGKFAKLTIEDNGKGMAQDALANSSSLGIES
EEEEEECHHHHHHHHHHCCHHHHHHEECCCCCEEEEEEECCCCCHHHHHHCCCCCCCHHH
MLERAALIGGTIDFTVGHHQCGFSVKLVWPIPEDMIN
HHHHHHHHCCCEEEEECCCCCCEEEEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]