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The map label for this gene is leuO [H]

Identifier: 157961551

GI number: 157961551

Start: 2099532

End: 2100446

Strand: Direct

Name: leuO [H]

Synonym: Spea_1727

Alternate gene names: 157961551

Gene position: 2099532-2100446 (Clockwise)

Preceding gene: 157961546

Following gene: 157961559

Centisome position: 40.57

GC content: 44.26

Gene sequence:

>915_bases
GTGGGCAACAATATGGATAAAAGATTCGATTTCAATTTGTTAGTGATTTTTTTAGAGGTTTACCAGCTTAGATCTATTAC
TCTAGCTGCTGACTCCTTAGGTTTAACTCAGCCGGGCGTCAGTGCGGCATTAAAACGCCTACAAGCTACACTCGGAGTAG
AGCTTTTTGTTCGTGAAGGTCGTGGTATTTCACCAACCAACGCGGCGATTCAGCTAGCGAGTCAAGTCTCACCGGCCTAC
AACACGGTTAACAGTGCACTGAGCAATGTGGCAGGTTTTGACAAGCAGACACCGCGCTCGTTTCTAGTCTATGTCGATGA
ACCTATGATGAATCTGCTGCAACCTTTAGCTGAACAAGATAGCGATATGGGCAACTGTGAGATTATGTTTCAACTGCCCC
CGATTGAAGAGGAACGACTGCTACAGCAGTTGAGTTTGCAAAAGGCTGACTTAGCGATAGACGTTGGACAGTTGCCTAGC
CTTTCTTACTCTAATCAACTTTTTTACCGTGAAAGCGTCACCATGATTTGCCGTAAGGGTCATCCGCGTATTAATGGCAA
GATAGGCAAAATTCAGTTTTACCAAGAGAAACATATTACTTTGCAGATCCGCCGCGCGACGCTAACTGCACTGGATTTTT
TCACCGAGGAAGTGATCGGTGAACGTAAGGTTAGCTGTGAGTGTCACTCATTACTCTCTATGATGGCGCTGGTTTCTGAA
AGTGATTGTATTGCTATCACCTCAAGGAGTATCGCTGATAAGTATGCAGATAAATTTGGTCTTCAAAGTATCGAGCCGCC
CTTTAACACTAAGCCTGTGCTACATAATATGATGTGGCACAAACGTAATGAGTTTAACCCTGCACATACTTGGCTTAGGG
AAAAGCTAAACCAATTGGTGAAGCAATGGAATTAG

Upstream 100 bases:

>100_bases
TGAACCAATAAACTCAGCTTTAGTTGATACATAACTATTTCGTATGTATTGGCGTTATTATGTATAATTAATGCTTCTGT
ATTAGCGGTGAGAGAATTGC

Downstream 100 bases:

>100_bases
TTATATAGCTTATAATTCGTTAGCTTATTCCGCTAATCTTAGAGCCGTAAAATGGACTTCATAGAAATATACTAAGAGCT
ATTTTTTAGTGATGTAATCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MGNNMDKRFDFNLLVIFLEVYQLRSITLAADSLGLTQPGVSAALKRLQATLGVELFVREGRGISPTNAAIQLASQVSPAY
NTVNSALSNVAGFDKQTPRSFLVYVDEPMMNLLQPLAEQDSDMGNCEIMFQLPPIEEERLLQQLSLQKADLAIDVGQLPS
LSYSNQLFYRESVTMICRKGHPRINGKIGKIQFYQEKHITLQIRRATLTALDFFTEEVIGERKVSCECHSLLSMMALVSE
SDCIAITSRSIADKYADKFGLQSIEPPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLVKQWN

Sequences:

>Translated_304_residues
MGNNMDKRFDFNLLVIFLEVYQLRSITLAADSLGLTQPGVSAALKRLQATLGVELFVREGRGISPTNAAIQLASQVSPAY
NTVNSALSNVAGFDKQTPRSFLVYVDEPMMNLLQPLAEQDSDMGNCEIMFQLPPIEEERLLQQLSLQKADLAIDVGQLPS
LSYSNQLFYRESVTMICRKGHPRINGKIGKIQFYQEKHITLQIRRATLTALDFFTEEVIGERKVSCECHSLLSMMALVSE
SDCIAITSRSIADKYADKFGLQSIEPPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLVKQWN
>Mature_303_residues
GNNMDKRFDFNLLVIFLEVYQLRSITLAADSLGLTQPGVSAALKRLQATLGVELFVREGRGISPTNAAIQLASQVSPAYN
TVNSALSNVAGFDKQTPRSFLVYVDEPMMNLLQPLAEQDSDMGNCEIMFQLPPIEEERLLQQLSLQKADLAIDVGQLPSL
SYSNQLFYRESVTMICRKGHPRINGKIGKIQFYQEKHITLQIRRATLTALDFFTEEVIGERKVSCECHSLLSMMALVSES
DCIAITSRSIADKYADKFGLQSIEPPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLVKQWN

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=296, Percent_Identity=27.7027027027027, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081766, Length=295, Percent_Identity=24.0677966101695, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786820, Length=273, Percent_Identity=29.3040293040293, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI157672245, Length=261, Percent_Identity=24.5210727969349, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34413; Mature: 34282

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNMDKRFDFNLLVIFLEVYQLRSITLAADSLGLTQPGVSAALKRLQATLGVELFVREG
CCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCHHEEEECC
RGISPTNAAIQLASQVSPAYNTVNSALSNVAGFDKQTPRSFLVYVDEPMMNLLQPLAEQD
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHCC
SDMGNCEIMFQLPPIEEERLLQQLSLQKADLAIDVGQLPSLSYSNQLFYRESVTMICRKG
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEHHHHHHHHHHCC
HPRINGKIGKIQFYQEKHITLQIRRATLTALDFFTEEVIGERKVSCECHSLLSMMALVSE
CCCCCCEEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
SDCIAITSRSIADKYADKFGLQSIEPPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLV
CCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KQWN
HHCC
>Mature Secondary Structure 
GNNMDKRFDFNLLVIFLEVYQLRSITLAADSLGLTQPGVSAALKRLQATLGVELFVREG
CCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCHHEEEECC
RGISPTNAAIQLASQVSPAYNTVNSALSNVAGFDKQTPRSFLVYVDEPMMNLLQPLAEQD
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHCC
SDMGNCEIMFQLPPIEEERLLQQLSLQKADLAIDVGQLPSLSYSNQLFYRESVTMICRKG
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEHHHHHHHHHHCC
HPRINGKIGKIQFYQEKHITLQIRRATLTALDFFTEEVIGERKVSCECHSLLSMMALVSE
CCCCCCEEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
SDCIAITSRSIADKYADKFGLQSIEPPFNTKPVLHNMMWHKRNEFNPAHTWLREKLNQLV
CCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KQWN
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]