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The map label for this gene is xapA [H]

Identifier: 157961542

GI number: 157961542

Start: 2089906

End: 2090739

Strand: Reverse

Name: xapA [H]

Synonym: Spea_1718

Alternate gene names: 157961542

Gene position: 2090739-2089906 (Counterclockwise)

Preceding gene: 157961544

Following gene: 157961541

Centisome position: 40.4

GC content: 46.16

Gene sequence:

>834_bases
ATGTCTCTGTACGATCCAGTCCCGAACGCTATTGCTACCATCGAAAAGTACAAACCAGGTTTTAAGCCACAGATTGCGAT
GATCTTAGGCTCTGGTCTAGGAGTGCTTGCCGATAAACTCGAAGATAAAGTTAGCATCGGTTATCAAGAGCTTGATGGTT
TCCCTGTTAGCACCGTTGAAGGTCACAGTGGTGAGTTGGTACTTGGTTACCTAAACGGCGTACCTGTTGCTTGTATGAAG
GGCCGTGGTCACTTCTATGAACATGAGTCAATGAAAGTCATGACGACACCCGTGCGCACTTTCAAGACGCTGGGTTGCGA
GATGTTATTAGTTACCAATGCTGCGGGCTCTTTACGTCCAGATCGCATCGATGTTGGCTCTCTCGTTGTGTTCTCAGATC
ACATAAACACCATGCCAAGCACCCCAATGACAGGACCTAATGACGACAAGTACGGTCCACGTTTCTTCAGTCTTGCCAAT
GCCTATGACAAAGATTTACGCGAGCAAGCTTTAGGCATAGCTAAAACACAAAATATCGTACTCAACCAAGGCATTTTTGT
TTCTTACCCAGGTCCCTGTTTCGAAACCGCTGCTGAAATTCGCATGATGCAAATCATCGGTGGAGATGTAGTAGGCATGT
CAGTCGTACCAGAAGTTATCTCTGCAGCGCATTGCGATCTACCCGTACTTGCTATCTGTGCGATCACCAACATGGCTGAA
GGCTTAGGTGATGTGAAGCTATCACATGAGCAAACTCTTAAGTGTGCAAAACTGGCTGAAAGTGACTTCTTACAGCTAAT
CAATGCATTCGTCGATAGCGTTAAGAAAGCGTAG

Upstream 100 bases:

>100_bases
TTGTGCCGAAAATCAGCACATTAGAGTCGATTTTAGCGATTTTAATCAAAATAGCTTGGGAATATCGTGAGCCCAATAAC
AAATCCAATTAGGAATAAAA

Downstream 100 bases:

>100_bases
AGGATAGGACTATGATGTTCATGGGATTTATCGGCATGCTAGTGCTTTTAGGCATAGGCTTCGCGTGCTCAGTTAGTCGA
CGTTCAATTAAACTACGTAC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSLYDPVPNAIATIEKYKPGFKPQIAMILGSGLGVLADKLEDKVSIGYQELDGFPVSTVEGHSGELVLGYLNGVPVACMK
GRGHFYEHESMKVMTTPVRTFKTLGCEMLLVTNAAGSLRPDRIDVGSLVVFSDHINTMPSTPMTGPNDDKYGPRFFSLAN
AYDKDLREQALGIAKTQNIVLNQGIFVSYPGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCDLPVLAICAITNMAE
GLGDVKLSHEQTLKCAKLAESDFLQLINAFVDSVKKA

Sequences:

>Translated_277_residues
MSLYDPVPNAIATIEKYKPGFKPQIAMILGSGLGVLADKLEDKVSIGYQELDGFPVSTVEGHSGELVLGYLNGVPVACMK
GRGHFYEHESMKVMTTPVRTFKTLGCEMLLVTNAAGSLRPDRIDVGSLVVFSDHINTMPSTPMTGPNDDKYGPRFFSLAN
AYDKDLREQALGIAKTQNIVLNQGIFVSYPGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCDLPVLAICAITNMAE
GLGDVKLSHEQTLKCAKLAESDFLQLINAFVDSVKKA
>Mature_276_residues
SLYDPVPNAIATIEKYKPGFKPQIAMILGSGLGVLADKLEDKVSIGYQELDGFPVSTVEGHSGELVLGYLNGVPVACMKG
RGHFYEHESMKVMTTPVRTFKTLGCEMLLVTNAAGSLRPDRIDVGSLVVFSDHINTMPSTPMTGPNDDKYGPRFFSLANA
YDKDLREQALGIAKTQNIVLNQGIFVSYPGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCDLPVLAICAITNMAEG
LGDVKLSHEQTLKCAKLAESDFLQLINAFVDSVKKA

Specific function: The nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the nucleoside molecule, with the formation of the corresponding free bases and pentose-1-phosphate. This protein can degrade all purine nucleosides except ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=263, Percent_Identity=43.3460076045627, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1788746, Length=264, Percent_Identity=65.9090909090909, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17541190, Length=265, Percent_Identity=35.4716981132075, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323238, Length=271, Percent_Identity=39.8523985239852, Blast_Score=169, Evalue=4e-43,
Organism=Drosophila melanogaster, GI45552887, Length=267, Percent_Identity=40.8239700374532, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24656093, Length=267, Percent_Identity=40.8239700374532, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24656090, Length=267, Percent_Identity=40.8239700374532, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45552885, Length=267, Percent_Identity=40.8239700374532, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24762376, Length=224, Percent_Identity=34.8214285714286, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR010943
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.-

Molecular weight: Translated: 29831; Mature: 29700

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYDPVPNAIATIEKYKPGFKPQIAMILGSGLGVLADKLEDKVSIGYQELDGFPVSTVE
CCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCCEEEC
GHSGELVLGYLNGVPVACMKGRGHFYEHESMKVMTTPVRTFKTLGCEMLLVTNAAGSLRP
CCCCCEEEEECCCCCEEEECCCCCEEECCCCEEEECHHHHHHHCCCEEEEEECCCCCCCC
DRIDVGSLVVFSDHINTMPSTPMTGPNDDKYGPRFFSLANAYDKDLREQALGIAKTQNIV
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCEE
LNQGIFVSYPGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCDLPVLAICAITNMAE
EECCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GLGDVKLSHEQTLKCAKLAESDFLQLINAFVDSVKKA
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLYDPVPNAIATIEKYKPGFKPQIAMILGSGLGVLADKLEDKVSIGYQELDGFPVSTVE
CCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCCEEEC
GHSGELVLGYLNGVPVACMKGRGHFYEHESMKVMTTPVRTFKTLGCEMLLVTNAAGSLRP
CCCCCEEEEECCCCCEEEECCCCCEEECCCCEEEECHHHHHHHCCCEEEEEECCCCCCCC
DRIDVGSLVVFSDHINTMPSTPMTGPNDDKYGPRFFSLANAYDKDLREQALGIAKTQNIV
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCEE
LNQGIFVSYPGPCFETAAEIRMMQIIGGDVVGMSVVPEVISAAHCDLPVLAICAITNMAE
EECCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GLGDVKLSHEQTLKCAKLAESDFLQLINAFVDSVKKA
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559336; 9205837; 9278503 [H]