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The map label for this gene is pdxB [H]

Identifier: 157961438

GI number: 157961438

Start: 1951920

End: 1953053

Strand: Direct

Name: pdxB [H]

Synonym: Spea_1612

Alternate gene names: 157961438

Gene position: 1951920-1953053 (Clockwise)

Preceding gene: 157961436

Following gene: 157961439

Centisome position: 37.72

GC content: 45.77

Gene sequence:

>1134_bases
ATGATGAAGATCCTTGCCGATGAAAATATGCCTTATGTTCAACAGTTATTTGGTGACTTAGGCACGATTGAAACCGTAAA
CGGTAGGGAGCTAACGCCTGAGCAAGTTAACGATGCCGATGTGTTACTCGTTCGCTCTGTGACTAAGGTGAATCAAGCCT
TATTGGCAGAGAATAACCAACTTAAGTTTGTTGGCAGTGCCACCATAGGCACAGATCATATCGATGTTGAATACCTTGCA
AGTCGCAATATTCCCTTCTCTAATGCGCCGGGCTGTAACGCCACCGCTGTGGGGGAGTTTGCCTTTATCGCCATGCTTGA
GCTTGCACAGCGCTTTAATAGCCCGCTAAAAGGCAAGGTGGTTGGTATTGTTGGTGCTGGCAACACTGGCACAGCGGTAG
CTAAATGCCTTGAGGCTTATGGCATTGAAGTTCTGCTTAATGACCCATTGTTAGAAGAGAGTGGCGATCCTCGTAGCTTT
GTATCACTCGATACCTTAATCGAAAAGTGCGATGTGATTAGCTTGCATGTACCGATAACTAAAACCGGTGAGCATAAAAC
TTGGTATCTGTTCGATGAGTCGAGACTTAATAAGCTAGCTGAAAACTGCTGGCTGCTTAATTGCTGCCGGGGCGAGGTGA
TTGATAACTGCGCCTTGATTAACTTCAAGCAACAGCGAGATGATGTAAAAGTGGTGCTTGATGTGTGGGAAGGTGAGCCT
AATCCTATGCCAGAGTTGGTGCCTTATGTGGAGTTTTGTACGCCCCATATCGCTGGCTACTCGTTAGAGGGCAAAGCGCG
CGGCACCTATATGCTCTATCAAAAGTTAGCTGAGGTGCTGAAGATTGAGGCCGACAAACAGATGGACTCGTTACTGCCGC
CGCTCTGGAGTGAGCAGGTTAACGTACAAGAAATCAGCGATGAAAGAGCACTTTTGCAGCTCGCGAGATTTGTATACAAT
TTGCGCGATGATGACGAATTATTTCGAAAAATGTTTCTAAATAAGCAGGGGTTTGATCATATGAGAAAAAATCACACCCA
TCGCCGCGAATTTAGTGCATTAAAGGTAGGCAACACGGGGCAAACTAATGTAAATTGGTTATCCAATTTAGGGTTTTCAG
GAGTCGAGCTGTAA

Upstream 100 bases:

>100_bases
ATAGACAGGATGAAAGCCTTTATTTTCAATCCTTGTTTCTAATAAATAGCCCATCTTGCTAGAATGCAGCCCATACTCAT
TCAGCACACTTTTGGAAACC

Downstream 100 bases:

>100_bases
TTATGTCGCAGGAATTTAATGTTGTCGTATTAGGTGCATCGGGCGCAGTGGGTCAAACAATGATTGAGATCCTCGAAGAG
CGTAACTTTCCGGTTGCTAA

Product: D-isomer specific 2-hydroxyacid dehydrogenase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MMKILADENMPYVQQLFGDLGTIETVNGRELTPEQVNDADVLLVRSVTKVNQALLAENNQLKFVGSATIGTDHIDVEYLA
SRNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTAVAKCLEAYGIEVLLNDPLLEESGDPRSF
VSLDTLIEKCDVISLHVPITKTGEHKTWYLFDESRLNKLAENCWLLNCCRGEVIDNCALINFKQQRDDVKVVLDVWEGEP
NPMPELVPYVEFCTPHIAGYSLEGKARGTYMLYQKLAEVLKIEADKQMDSLLPPLWSEQVNVQEISDERALLQLARFVYN
LRDDDELFRKMFLNKQGFDHMRKNHTHRREFSALKVGNTGQTNVNWLSNLGFSGVEL

Sequences:

>Translated_377_residues
MMKILADENMPYVQQLFGDLGTIETVNGRELTPEQVNDADVLLVRSVTKVNQALLAENNQLKFVGSATIGTDHIDVEYLA
SRNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTAVAKCLEAYGIEVLLNDPLLEESGDPRSF
VSLDTLIEKCDVISLHVPITKTGEHKTWYLFDESRLNKLAENCWLLNCCRGEVIDNCALINFKQQRDDVKVVLDVWEGEP
NPMPELVPYVEFCTPHIAGYSLEGKARGTYMLYQKLAEVLKIEADKQMDSLLPPLWSEQVNVQEISDERALLQLARFVYN
LRDDDELFRKMFLNKQGFDHMRKNHTHRREFSALKVGNTGQTNVNWLSNLGFSGVEL
>Mature_377_residues
MMKILADENMPYVQQLFGDLGTIETVNGRELTPEQVNDADVLLVRSVTKVNQALLAENNQLKFVGSATIGTDHIDVEYLA
SRNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTAVAKCLEAYGIEVLLNDPLLEESGDPRSF
VSLDTLIEKCDVISLHVPITKTGEHKTWYLFDESRLNKLAENCWLLNCCRGEVIDNCALINFKQQRDDVKVVLDVWEGEP
NPMPELVPYVEFCTPHIAGYSLEGKARGTYMLYQKLAEVLKIEADKQMDSLLPPLWSEQVNVQEISDERALLQLARFVYN
LRDDDELFRKMFLNKQGFDHMRKNHTHRREFSALKVGNTGQTNVNWLSNLGFSGVEL

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=294, Percent_Identity=24.4897959183673, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI145580578, Length=287, Percent_Identity=22.9965156794425, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4557499, Length=287, Percent_Identity=22.9965156794425, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI145580575, Length=159, Percent_Identity=30.8176100628931, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI61743967, Length=271, Percent_Identity=23.6162361623616, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4557497, Length=271, Percent_Identity=23.6162361623616, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788660, Length=375, Percent_Identity=44.2666666666667, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1789279, Length=263, Percent_Identity=28.1368821292776, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87082289, Length=300, Percent_Identity=25, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787645, Length=199, Percent_Identity=24.1206030150754, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=251, Percent_Identity=26.2948207171315, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322116, Length=267, Percent_Identity=26.5917602996255, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=267, Percent_Identity=26.5917602996255, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=240, Percent_Identity=24.5833333333333, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324964, Length=283, Percent_Identity=22.2614840989399, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19921140, Length=262, Percent_Identity=26.3358778625954, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24646446, Length=238, Percent_Identity=26.4705882352941, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646448, Length=238, Percent_Identity=26.4705882352941, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646452, Length=238, Percent_Identity=26.4705882352941, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646450, Length=238, Percent_Identity=26.4705882352941, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574284, Length=244, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45551003, Length=244, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585514, Length=244, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574282, Length=244, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552429, Length=244, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI62472511, Length=238, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28574286, Length=239, Percent_Identity=25.9414225941423, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 42244; Mature: 42244

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKILADENMPYVQQLFGDLGTIETVNGRELTPEQVNDADVLLVRSVTKVNQALLAENNQ
CCEEEECCCCCHHHHHHCCCCCEEECCCCCCCHHHCCCCCEEEHHHHHHHHHHHHCCCCC
LKFVGSATIGTDHIDVEYLASRNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKV
EEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEE
VGIVGAGNTGTAVAKCLEAYGIEVLLNDPLLEESGDPRSFVSLDTLIEKCDVISLHVPIT
EEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECEE
KTGEHKTWYLFDESRLNKLAENCWLLNCCRGEVIDNCALINFKQQRDDVKVVLDVWEGEP
ECCCCCEEEEECHHHHHHHHHHCEEEECCCCHHHCCEEEEEEHHCCCCEEEEEEEECCCC
NPMPELVPYVEFCTPHIAGYSLEGKARGTYMLYQKLAEVLKIEADKQMDSLLPPLWSEQV
CCCHHHHHHHHHHCHHHCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHCCCC
NVQEISDERALLQLARFVYNLRDDDELFRKMFLNKQGFDHMRKNHTHRREFSALKVGNTG
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHCEEECCCCC
QTNVNWLSNLGFSGVEL
CCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MMKILADENMPYVQQLFGDLGTIETVNGRELTPEQVNDADVLLVRSVTKVNQALLAENNQ
CCEEEECCCCCHHHHHHCCCCCEEECCCCCCCHHHCCCCCEEEHHHHHHHHHHHHCCCCC
LKFVGSATIGTDHIDVEYLASRNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKV
EEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEE
VGIVGAGNTGTAVAKCLEAYGIEVLLNDPLLEESGDPRSFVSLDTLIEKCDVISLHVPIT
EEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECEE
KTGEHKTWYLFDESRLNKLAENCWLLNCCRGEVIDNCALINFKQQRDDVKVVLDVWEGEP
ECCCCCEEEEECHHHHHHHHHHCEEEECCCCHHHCCEEEEEEHHCCCCEEEEEEEECCCC
NPMPELVPYVEFCTPHIAGYSLEGKARGTYMLYQKLAEVLKIEADKQMDSLLPPLWSEQV
CCCHHHHHHHHHHCHHHCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHCCCC
NVQEISDERALLQLARFVYNLRDDDELFRKMFLNKQGFDHMRKNHTHRREFSALKVGNTG
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCHHHHHCEEECCCCC
QTNVNWLSNLGFSGVEL
CCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA