Click here to switch to the map view.

The map label for this gene is trpG [H]

Identifier: 157961415

GI number: 157961415

Start: 1927995

End: 1928609

Strand: Reverse

Name: trpG [H]

Synonym: Spea_1589

Alternate gene names: 157961415

Gene position: 1928609-1927995 (Counterclockwise)

Preceding gene: 157961416

Following gene: 157961414

Centisome position: 37.27

GC content: 47.32

Gene sequence:

>615_bases
ATGAAAATCTATCTACTCGATAACTTCGACTCATTCACCTACAACTTAGTGGATCAGTTTAGAAGCTTAGGCTATGAAGT
AGTCATTTATCGTAACGACGTCGACGCTGACTTTATCGCCAGTAAACTATTAAACGAAACAACACCCGCCGCACTTGTAC
TGTCGCCAGGTCCCGGAGCACCTCACGAAGCAGGCTCATTAATGGCTCTGATTGAGTGTGTTGCAGGCAAAGTACCAATG
CTGGGTATTTGCCTTGGGCACCAAGCCTTAGTTGAGCATTACGGCGGTAAAGTTGAGCGTGCAGCTCAAGTGGTACACGG
CAAAGCGAGTCCGACATTTCATAACGGCCAAGATATCTTTAGCGGTTTACCCTCGCCGCTTCCCGTTGCCCGTTATCACA
GCCTTGTGGCAACCAAAGTGCCGGATTGCTTAGACGTTATCGCCACTACAGAAGAGATGCCAATGGCGGTTATTCACCGC
GAAGATATAGCGGTGGGTTTTCAGTTCCATCCGGAATCAATTTTAACCACTTTAGGCAGCCAGTTATTGGTACAGAGCTT
AGATTACCTCGCCAGTGCTTTAGCACAAGATAGCCAAGACAACATCAAAGGATAA

Upstream 100 bases:

>100_bases
GCGCTGGCGTGGTGTACGACTCAGACCCACAAGCCGAAGCCGATGAAACGCGCCAAAAGGCACAAGCCGTTATTTCAGCT
ATAAAAATGGGAGGTGGACT

Downstream 100 bases:

>100_bases
GATGATGACTCAAACCGAAATCAAGCAGCTTGAAATTAAAAGCTCTGAAACGTTAATCGAATCCCTTTATCGCGGTGAAA
GCCTAAACCGCACTGATGCA

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKIYLLDNFDSFTYNLVDQFRSLGYEVVIYRNDVDADFIASKLLNETTPAALVLSPGPGAPHEAGSLMALIECVAGKVPM
LGICLGHQALVEHYGGKVERAAQVVHGKASPTFHNGQDIFSGLPSPLPVARYHSLVATKVPDCLDVIATTEEMPMAVIHR
EDIAVGFQFHPESILTTLGSQLLVQSLDYLASALAQDSQDNIKG

Sequences:

>Translated_204_residues
MKIYLLDNFDSFTYNLVDQFRSLGYEVVIYRNDVDADFIASKLLNETTPAALVLSPGPGAPHEAGSLMALIECVAGKVPM
LGICLGHQALVEHYGGKVERAAQVVHGKASPTFHNGQDIFSGLPSPLPVARYHSLVATKVPDCLDVIATTEEMPMAVIHR
EDIAVGFQFHPESILTTLGSQLLVQSLDYLASALAQDSQDNIKG
>Mature_204_residues
MKIYLLDNFDSFTYNLVDQFRSLGYEVVIYRNDVDADFIASKLLNETTPAALVLSPGPGAPHEAGSLMALIECVAGKVPM
LGICLGHQALVEHYGGKVERAAQVVHGKASPTFHNGQDIFSGLPSPLPVARYHSLVATKVPDCLDVIATTEEMPMAVIHR
EDIAVGFQFHPESILTTLGSQLLVQSLDYLASALAQDSQDNIKG

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=192, Percent_Identity=54.6875, Blast_Score=208, Evalue=2e-55,
Organism=Escherichia coli, GI1789760, Length=190, Percent_Identity=49.4736842105263, Blast_Score=173, Evalue=6e-45,
Organism=Escherichia coli, GI1788854, Length=191, Percent_Identity=26.7015706806283, Blast_Score=66, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322638, Length=203, Percent_Identity=37.4384236453202, Blast_Score=142, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324361, Length=208, Percent_Identity=31.25, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 22020; Mature: 22020

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLLDNFDSFTYNLVDQFRSLGYEVVIYRNDVDADFIASKLLNETTPAALVLSPGPGA
CEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCC
PHEAGSLMALIECVAGKVPMLGICLGHQALVEHYGGKVERAAQVVHGKASPTFHNGQDIF
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHH
SGLPSPLPVARYHSLVATKVPDCLDVIATTEEMPMAVIHREDIAVGFQFHPESILTTLGS
HCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHEEECCCEEEEEEECHHHHHHHHHH
QLLVQSLDYLASALAQDSQDNIKG
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKIYLLDNFDSFTYNLVDQFRSLGYEVVIYRNDVDADFIASKLLNETTPAALVLSPGPGA
CEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCC
PHEAGSLMALIECVAGKVPMLGICLGHQALVEHYGGKVERAAQVVHGKASPTFHNGQDIF
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHH
SGLPSPLPVARYHSLVATKVPDCLDVIATTEEMPMAVIHREDIAVGFQFHPESILTTLGS
HCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHEEECCCEEEEEEECHHHHHHHHHH
QLLVQSLDYLASALAQDSQDNIKG
HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]