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The map label for this gene is trpA [H]

Identifier: 157961411

GI number: 157961411

Start: 1923342

End: 1924151

Strand: Reverse

Name: trpA [H]

Synonym: Spea_1585

Alternate gene names: 157961411

Gene position: 1924151-1923342 (Counterclockwise)

Preceding gene: 157961412

Following gene: 157961398

Centisome position: 37.18

GC content: 50.86

Gene sequence:

>810_bases
ATGAGTCAACGTTACCAGGCAGCCTTTGCCGCCCTAAATGAGAAACAGCAAGGCGCATTTGTGCCATTCGTGACCTTAGG
CGATCCATCGCCTGAGCTATCACTTAAAATCATCGACACCTTAGTTGAAAACGGTGCCGACGCCCTAGAGCTCGGCTTCC
CGTTTTCAGATCCGCTCGCCGATGGCCCGGTTATTCAAGGCGCTAACCTACGCTCTTTAGCTGCTGGCACTACACCGACT
CAGTGCTTTGAAATGCTCACGGCTATTCGAGCTAAATATCCTGACTTACCGATTGGCCTACTACTGTACGCCAATTTAGT
GTTCGCCAATGGCATCGATACCTTCTACGCCAAATGCCAAGCCGCTGGCGTTGACTCGGTACTGATTGCCGATGTGCCAG
TAGAGGAAGCAGCACCATTTATCGAATCGGCAAAAGCCCACGGCGTGTGCCCAATCTTTATCGCGCCGCCAAATGCCGAC
AGCGAAACATTAAAACAGGTAAGTGAAAAAGGTGAAGGTTATACCTACCTGTTATCTCGCGCTGGTGTGACGGGTACGGA
GTCAAAGGCCGGTATGCCAATCAGTGATATTCTAAATCGTCTAAAAGAGTTTAATGGTGCGCCGCCACTACTAGGTTTTG
GTATTGCCGAGCCGAGTCAGGTCAAAGCCGCTATCGATGCTGGAGCCGCGGGCGCGATTTCAGGCTCTGCGGTAGTCAAA
ATTATCGAGGCGAATAAAGACAATGAAGCGGCACTGTTAAGCGCCCTTGCTGAATTTACTCGCAATATGAAAGCGGCAAC
CGCTCGTTAA

Upstream 100 bases:

>100_bases
GATATCTTCACGGTTGCGGACATTCTTGAGCAACAAAAAGTTGAGCAACAAGAAGCTGAACAGCAACAAACTAACAATCA
GAACAACTAGGAGAGTGGCA

Downstream 100 bases:

>100_bases
AGCACCGCTGCATCTTGCTCTGCTTTAGCTAAGAGTTACAGATATAAAAATAGCGCCTTAAGGCGCTATTTTTGTTTGTG
TGCTCGATTTAATAACCGGT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSQRYQAAFAALNEKQQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLADGPVIQGANLRSLAAGTTPT
QCFEMLTAIRAKYPDLPIGLLLYANLVFANGIDTFYAKCQAAGVDSVLIADVPVEEAAPFIESAKAHGVCPIFIAPPNAD
SETLKQVSEKGEGYTYLLSRAGVTGTESKAGMPISDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVK
IIEANKDNEAALLSALAEFTRNMKAATAR

Sequences:

>Translated_269_residues
MSQRYQAAFAALNEKQQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLADGPVIQGANLRSLAAGTTPT
QCFEMLTAIRAKYPDLPIGLLLYANLVFANGIDTFYAKCQAAGVDSVLIADVPVEEAAPFIESAKAHGVCPIFIAPPNAD
SETLKQVSEKGEGYTYLLSRAGVTGTESKAGMPISDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVK
IIEANKDNEAALLSALAEFTRNMKAATAR
>Mature_268_residues
SQRYQAAFAALNEKQQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLADGPVIQGANLRSLAAGTTPTQ
CFEMLTAIRAKYPDLPIGLLLYANLVFANGIDTFYAKCQAAGVDSVLIADVPVEEAAPFIESAKAHGVCPIFIAPPNADS
ETLKQVSEKGEGYTYLLSRAGVTGTESKAGMPISDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVKI
IEANKDNEAALLSALAEFTRNMKAATAR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=265, Percent_Identity=66.4150943396226, Blast_Score=345, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6321412, Length=242, Percent_Identity=30.5785123966942, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 28045; Mature: 27914

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRYQAAFAALNEKQQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLA
CCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPVIQGANLRSLAAGTTPTQCFEMLTAIRAKYPDLPIGLLLYANLVFANGIDTFYAKCQ
CCCEECCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
AAGVDSVLIADVPVEEAAPFIESAKAHGVCPIFIAPPNADSETLKQVSEKGEGYTYLLSR
HCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
AGVTGTESKAGMPISDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVK
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCEEEE
IIEANKDNEAALLSALAEFTRNMKAATAR
EEECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQRYQAAFAALNEKQQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLA
CCHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPVIQGANLRSLAAGTTPTQCFEMLTAIRAKYPDLPIGLLLYANLVFANGIDTFYAKCQ
CCCEECCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
AAGVDSVLIADVPVEEAAPFIESAKAHGVCPIFIAPPNADSETLKQVSEKGEGYTYLLSR
HCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
AGVTGTESKAGMPISDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVK
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCEEEE
IIEANKDNEAALLSALAEFTRNMKAATAR
EEECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]