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The map label for this gene is nanA2 [H]

Identifier: 157961348

GI number: 157961348

Start: 1849545

End: 1850450

Strand: Reverse

Name: nanA2 [H]

Synonym: Spea_1522

Alternate gene names: 157961348

Gene position: 1850450-1849545 (Counterclockwise)

Preceding gene: 157961349

Following gene: 157961342

Centisome position: 35.76

GC content: 44.37

Gene sequence:

>906_bases
ATGCAAAAACTAACTGGGTTAATCGCAGCACCACATACTCCATTTGATATTAATGGTGATGTTAATCTGCCTGTTATCGA
TCAAATTGCCGCACTGCTCATCAAACAAAATGTTAAAGGTGTTTATATTTGCGGCACAACAGGCGAAGGCCTCAATTGCT
CCGTCAATGAGCGAAAAGCGGTGGCAGAGCGCTGGGTTAAGGCCGCACAAGGTAAACTAAGTATTACAGTTCATACTGGC
GCTCTATCAATTGTTGACACGCTAGAACTAACTAAGCACGCCGATACTCTTGATGTATTTGCAACATCCGTTATTGCACC
TTGCTTTTTTAAACCCAGTTCAGTCGATTGCCTTGTCAAATACTGCGCAACCGTCGCGGCCGCGGCGCCAAGTAAAGCTT
TTTATTACTACCACTCAGGAATGTCGAATGTCATGTTTGATATGGAACAGTTTCTTATCAAAGCAGATAAAGTCATCGAT
AACCTTGCAGGTATAAAATTTAACCATGTCGATTTATATGAGTTTCAGCGCTGTAAGCGAGTAGCCAATGGTAAATTCGA
TATTCCATTTGGTGTCGATGAGTCACTGCCAGGCGGGCTCGCAGTAGGCGCTATCGGCGCTGTTGGTAGTACCTATAATT
ACGCAGCCTCGCTGTATTTGGACATAATTGATGCGTTTAACAAAGGAGAGCAACAAACTGTCACTCGCTTAATGAATAAA
GTTATTGGCCTTATTCGACCTCTTGTGCAATATGGCGGAGTCGCGGCGGGGAAAGCGGCGATGCAACTGCATGGGATTGA
TGTTGGCGATCCTCGTATGCCAATTCGAGCCCTATCACCACAAGAAAAAGAAGATGTCATAGTGAAAATGAAAGCCGCCG
GCTTTATGGAGCATACAGCTTCATAA

Upstream 100 bases:

>100_bases
AGACTTCACCGTACTTGAAAAGAGTAGTCAAACAAGTTTACACGTAAGCCAAACATGGATTGATGGCAAACAAATATTTT
AATTTAAATAAAGGATTACC

Downstream 100 bases:

>100_bases
CACCATGATGCTGATGCTATCGCTTATTTCTTACTTATATTTATGTTTAAAGGCCAAGTTGGTAGCTTTAACCACCCATT
TGGCCAACCCAATAAGTAAT

Product: dihydrodipicolinate synthetase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase 2; N-acetylneuraminic acid aldolase 2; Sialate lyase 2; Sialic acid aldolase 2; Sialic acid lyase 2 [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MQKLTGLIAAPHTPFDINGDVNLPVIDQIAALLIKQNVKGVYICGTTGEGLNCSVNERKAVAERWVKAAQGKLSITVHTG
ALSIVDTLELTKHADTLDVFATSVIAPCFFKPSSVDCLVKYCATVAAAAPSKAFYYYHSGMSNVMFDMEQFLIKADKVID
NLAGIKFNHVDLYEFQRCKRVANGKFDIPFGVDESLPGGLAVGAIGAVGSTYNYAASLYLDIIDAFNKGEQQTVTRLMNK
VIGLIRPLVQYGGVAAGKAAMQLHGIDVGDPRMPIRALSPQEKEDVIVKMKAAGFMEHTAS

Sequences:

>Translated_301_residues
MQKLTGLIAAPHTPFDINGDVNLPVIDQIAALLIKQNVKGVYICGTTGEGLNCSVNERKAVAERWVKAAQGKLSITVHTG
ALSIVDTLELTKHADTLDVFATSVIAPCFFKPSSVDCLVKYCATVAAAAPSKAFYYYHSGMSNVMFDMEQFLIKADKVID
NLAGIKFNHVDLYEFQRCKRVANGKFDIPFGVDESLPGGLAVGAIGAVGSTYNYAASLYLDIIDAFNKGEQQTVTRLMNK
VIGLIRPLVQYGGVAAGKAAMQLHGIDVGDPRMPIRALSPQEKEDVIVKMKAAGFMEHTAS
>Mature_301_residues
MQKLTGLIAAPHTPFDINGDVNLPVIDQIAALLIKQNVKGVYICGTTGEGLNCSVNERKAVAERWVKAAQGKLSITVHTG
ALSIVDTLELTKHADTLDVFATSVIAPCFFKPSSVDCLVKYCATVAAAAPSKAFYYYHSGMSNVMFDMEQFLIKADKVID
NLAGIKFNHVDLYEFQRCKRVANGKFDIPFGVDESLPGGLAVGAIGAVGSTYNYAASLYLDIIDAFNKGEQQTVTRLMNK
VIGLIRPLVQYGGVAAGKAAMQLHGIDVGDPRMPIRALSPQEKEDVIVKMKAAGFMEHTAS

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=307, Percent_Identity=39.413680781759, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1789620, Length=245, Percent_Identity=28.9795918367347, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786463, Length=233, Percent_Identity=26.1802575107296, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082415, Length=306, Percent_Identity=24.1830065359477, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 32404; Mature: 32404

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00665 DHDPS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLTGLIAAPHTPFDINGDVNLPVIDQIAALLIKQNVKGVYICGTTGEGLNCSVNERKA
CCCHHCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHH
VAERWVKAAQGKLSITVHTGALSIVDTLELTKHADTLDVFATSVIAPCFFKPSSVDCLVK
HHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHH
YCATVAAAAPSKAFYYYHSGMSNVMFDMEQFLIKADKVIDNLAGIKFNHVDLYEFQRCKR
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHH
VANGKFDIPFGVDESLPGGLAVGAIGAVGSTYNYAASLYLDIIDAFNKGEQQTVTRLMNK
HHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VIGLIRPLVQYGGVAAGKAAMQLHGIDVGDPRMPIRALSPQEKEDVIVKMKAAGFMEHTA
HHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCCCEECCCCCCCCCEEEEEECCCCHHHCC
S
C
>Mature Secondary Structure
MQKLTGLIAAPHTPFDINGDVNLPVIDQIAALLIKQNVKGVYICGTTGEGLNCSVNERKA
CCCHHCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHH
VAERWVKAAQGKLSITVHTGALSIVDTLELTKHADTLDVFATSVIAPCFFKPSSVDCLVK
HHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHH
YCATVAAAAPSKAFYYYHSGMSNVMFDMEQFLIKADKVIDNLAGIKFNHVDLYEFQRCKR
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHH
VANGKFDIPFGVDESLPGGLAVGAIGAVGSTYNYAASLYLDIIDAFNKGEQQTVTRLMNK
HHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VIGLIRPLVQYGGVAAGKAAMQLHGIDVGDPRMPIRALSPQEKEDVIVKMKAAGFMEHTA
HHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCCCEECCCCCCCCCEEEEEECCCCHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]