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The map label for this gene is trmJ [H]

Identifier: 157961311

GI number: 157961311

Start: 1807534

End: 1808298

Strand: Direct

Name: trmJ [H]

Synonym: Spea_1485

Alternate gene names: 157961311

Gene position: 1807534-1808298 (Clockwise)

Preceding gene: 157961307

Following gene: 157961312

Centisome position: 34.93

GC content: 45.75

Gene sequence:

>765_bases
ATGCTCAGCAATATTCGCGTCGTTCTTGTCGGTACTTCCCATCCAGGGAATATAGGTTCTACAGCCCGTGCTATGAAAAC
CATGGGGTTATCCACTTTATATTTGGCAGAGCCAAAGGTTGAGCCAGATGGCCACTCTATTGCGTTAGCCGCTGGTGCTT
CAGATATTCTTAAACATGCTATTACCGTTAGCTCTGTAGAAGAAGCCATCGCCGATTGTAGCCTTGTTATCGCAACCAGT
GCTCGTAGTCGCACTTTGGACTGGCCAATGCTTGAGCCACGTGAAGCGGGTGAGAAGCTAGTGGGTTCTGCGCTTAATGG
CCCTGTGGCGATTGTATTTGGTCGTGAGAATAACGGTTTGACTAATGAAGAGCTACAGCGCTGTGATTACCACGTCGCTA
TTCCGGCAAACCCTGAATATACTTCTTTGAATCTGGCCCAAGCGGTACAGATTATCTGTTATGAGGCGCGAGTGGCACAT
CTTGCTCAAGTGCAAAACTCTGCAGAAGCTGTGATCGAAACAGAAAGTGAAGAGGCTCAAGAAGACTTTCCATCATCAAA
AGAGCGTGAGAATTTCTTCGAGCATCTAGAGAATACGCTTTTTTCGACAAACTTTATCGTCAAAAACCATCCTGGCCAGA
TAATGACTAAGTTACGTCGCCTATTTAGCCGTACCCGAATTGAACGCCAAGAGATGAATATCTTGCGCGGTATATTGACC
TCGATTGATAAAGTCGTCGCTAAAAGTGAGACTAAAGATAAGTAA

Upstream 100 bases:

>100_bases
TCTATTTGACGGTTATATCAAATGCTGATTTTTGTATAAGCAAAATGAGTCGCCTGTGGTAACATCTTGTCACGTGTTTT
CTCAAAAATTAAGTAGTTTC

Downstream 100 bases:

>100_bases
ACAGCGTTAAGCAAAGGAAGTAGCTAAATGGGTGTTATCGCAAGACTGAAAGACGACATAGCCTCTATTTACCATAGAGA
TCCAGCCGCAAATGGAACCA

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHAITVSSVEEAIADCSLVIATS
ARSRTLDWPMLEPREAGEKLVGSALNGPVAIVFGRENNGLTNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAH
LAQVQNSAEAVIETESEEAQEDFPSSKERENFFEHLENTLFSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILT
SIDKVVAKSETKDK

Sequences:

>Translated_254_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHAITVSSVEEAIADCSLVIATS
ARSRTLDWPMLEPREAGEKLVGSALNGPVAIVFGRENNGLTNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAH
LAQVQNSAEAVIETESEEAQEDFPSSKERENFFEHLENTLFSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILT
SIDKVVAKSETKDK
>Mature_254_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHAITVSSVEEAIADCSLVIATS
ARSRTLDWPMLEPREAGEKLVGSALNGPVAIVFGRENNGLTNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAH
LAQVQNSAEAVIETESEEAQEDFPSSKERENFFEHLENTLFSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILT
SIDKVVAKSETKDK

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=61.0655737704918, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1790865, Length=152, Percent_Identity=37.5, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27899; Mature: 27899

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHA
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHH
ITVSSVEEAIADCSLVIATSARSRTLDWPMLEPREAGEKLVGSALNGPVAIVFGRENNGL
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
TNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAHLAQVQNSAEAVIETESEEAQ
CHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHH
EDFPSSKERENFFEHLENTLFSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILT
HHCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIDKVVAKSETKDK
HHHHHHHCCCCCCC
>Mature Secondary Structure
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHA
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHH
ITVSSVEEAIADCSLVIATSARSRTLDWPMLEPREAGEKLVGSALNGPVAIVFGRENNGL
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
TNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAHLAQVQNSAEAVIETESEEAQ
CHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHH
EDFPSSKERENFFEHLENTLFSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILT
HHCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIDKVVAKSETKDK
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA