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The map label for this gene is gltX [H]

Identifier: 157961282

GI number: 157961282

Start: 1772396

End: 1773805

Strand: Direct

Name: gltX [H]

Synonym: Spea_1456

Alternate gene names: 157961282

Gene position: 1772396-1773805 (Clockwise)

Preceding gene: 157961280

Following gene: 157961284

Centisome position: 34.25

GC content: 43.48

Gene sequence:

>1410_bases
ATGACAGTTAAGACGCGTTTTGCTCCAAGCCCTACCGGCTTTTTGCACGTTGGTGGTGCTCGTACAGCACTTTATTCATG
GTTATATGCACGCGCAAACCAAGGTGAGTTTGTTTTACGTATCGAAGATACCGATCTTGAGCGTTCGACTCCAGAAGCAT
GCGCCGCAATTTTAGAAGGCATGGAATGGCTTAACCTGAATTGGGACGAAGGTCCTTACTATCAAACCAAGCGTTTTGAT
CGTTACAACGAAATCATTGCACAGATGCTAGAGCAGGGCACTGCATATAAGTGTTACTGTAGCCGTGAACGCATTGAAAC
AATGCGTGAAGAGCAAGCCGCGAAAGGTGAGCAACAAAAGTATGACGGTTGCTGCCGCGATAAAGCACCGCGTGATACTG
ATGAGCCATTTGTTGTGCGTTTCAAAAACCCAACAGAAGGTAGCGTTGTATTTGACGACCACGTACGTGGCCGTATTGAA
TTTGCAAACAGCACATTAGATGATTTGATCATCGCACGTACTGAAGGTACGCCTACTTACAACTTCTGTGTAGTTGTAGA
CGATTGGGATATGGGAATTACCTGCGTTGTTCGTGGTGAAGACCATATCAACAATACACCTCGTCAGATCAACATACTTA
AAGCATTAGGTGCACCAGTACCAGAATATGCTCACGTATCGATGATCTTAGGCGATGACGGCGCTAAGCTATCTAAACGT
CATGGTGCGGTAGGGGTGATGCAGTATCGTGATGACGGTTATTTACCAGAAGCGCTACTTAACTACTTAGTTCGCCTTGG
CTGGTCTCATGGCGATCAAGAAGTCTTCTCTATTGATGAGATGAAGCAATTCTTCAACCTTGATGATATCAACAAGGCGG
CGTCTGCTTTTAATACCGATAAACTGATTTGGTTAAACCAGCACTATATTAAAGAATCTGATCCGGAATATGTGGCTTCT
CACCTTGAGTGGCACATGGCTGATCAAAATATTGATACAAGCAATGGTCCAAAACTGTCTGAAGTGGTTTCTGCATTGTC
TGAACGTGCAAAGACATTAAAAGAACTTGCTGCATCTAGCCGCTACTTCTTTGAAGATTTTGCAGAGTTTGATGAAACTG
CCGCTAAAAAGCATCTAAGAGGTGTTGCACTTGAGCCACTAACTTTGTTCCAGACTAAATTAGCTGGATTAAATGATTGG
ACTTTAGAAGCTATTCATCAAGCAATTGAAGATACAGCAACTGAATTAGAAGTAGGAATGGGTAAAGTCGGTATGCCATT
ACGCGTAGCCGTAACGGGCGCTGGTATGTCTCCTGCTGTCGATTTAACCATATTCTTGGTTGGAAAGGCCCGTACAGAGC
AAAGAATCTCCAAAGCGATTGAATTTGTAGCAAATAGAATAAATTCGTAA

Upstream 100 bases:

>100_bases
AGTAGATCTATTTTCCCTAGCCCTTTTGGCTGTTAGAATATGCGCCACATTCATTAGTACAGTCTATTATTTAGGCTTTC
TACATAGGTCAATGTCCATT

Downstream 100 bases:

>100_bases
AAAACGGGTTGACTCTTTTGGGTGCGCTGCATAGAATGCGCCACGCAGTTGAGACACAGCCAACGAATAGTTCGCGGTGC
ACTCAAAAGTGATTTAGTTA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTVKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDLERSTPEACAAILEGMEWLNLNWDEGPYYQTKRFD
RYNEIIAQMLEQGTAYKCYCSRERIETMREEQAAKGEQQKYDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIE
FANSTLDDLIIARTEGTPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGAKLSKR
HGAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSIDEMKQFFNLDDINKAASAFNTDKLIWLNQHYIKESDPEYVAS
HLEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASSRYFFEDFAEFDETAAKKHLRGVALEPLTLFQTKLAGLNDW
TLEAIHQAIEDTATELEVGMGKVGMPLRVAVTGAGMSPAVDLTIFLVGKARTEQRISKAIEFVANRINS

Sequences:

>Translated_469_residues
MTVKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDLERSTPEACAAILEGMEWLNLNWDEGPYYQTKRFD
RYNEIIAQMLEQGTAYKCYCSRERIETMREEQAAKGEQQKYDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIE
FANSTLDDLIIARTEGTPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGAKLSKR
HGAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSIDEMKQFFNLDDINKAASAFNTDKLIWLNQHYIKESDPEYVAS
HLEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASSRYFFEDFAEFDETAAKKHLRGVALEPLTLFQTKLAGLNDW
TLEAIHQAIEDTATELEVGMGKVGMPLRVAVTGAGMSPAVDLTIFLVGKARTEQRISKAIEFVANRINS
>Mature_468_residues
TVKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDLERSTPEACAAILEGMEWLNLNWDEGPYYQTKRFDR
YNEIIAQMLEQGTAYKCYCSRERIETMREEQAAKGEQQKYDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIEF
ANSTLDDLIIARTEGTPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGAKLSKRH
GAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSIDEMKQFFNLDDINKAASAFNTDKLIWLNQHYIKESDPEYVASH
LEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASSRYFFEDFAEFDETAAKKHLRGVALEPLTLFQTKLAGLNDWT
LEAIHQAIEDTATELEVGMGKVGMPLRVAVTGAGMSPAVDLTIFLVGKARTEQRISKAIEFVANRINS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=500, Percent_Identity=30, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI62241042, Length=393, Percent_Identity=21.882951653944, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788743, Length=469, Percent_Identity=72.2814498933902, Blast_Score=728, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=240, Percent_Identity=40.8333333333333, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1786895, Length=227, Percent_Identity=25.9911894273128, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=483, Percent_Identity=29.8136645962733, Blast_Score=201, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI17511007, Length=382, Percent_Identity=24.0837696335079, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=30.6122448979592, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321192, Length=231, Percent_Identity=30.3030303030303, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324742, Length=131, Percent_Identity=30.5343511450382, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=503, Percent_Identity=30.4174950298211, Blast_Score=240, Evalue=2e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 52852; Mature: 52721

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDLERSTPEACAAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCC
MEWLNLNWDEGPYYQTKRFDRYNEIIAQMLEQGTAYKCYCSRERIETMREEQAAKGEQQK
HHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCHHH
YDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIEFANSTLDDLIIARTEGTPTY
HCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCCCCEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSIDEMKQFFNLDDINKAASAFNTD
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCC
KLIWLNQHYIKESDPEYVASHLEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASS
EEEEECHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RYFFEDFAEFDETAAKKHLRGVALEPLTLFQTKLAGLNDWTLEAIHQAIEDTATELEVGM
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GKVGMPLRVAVTGAGMSPAVDLTIFLVGKARTEQRISKAIEFVANRINS
CCCCCCEEEEEECCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVKTRFAPSPTGFLHVGGARTALYSWLYARANQGEFVLRIEDTDLERSTPEACAAILEG
CCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCC
MEWLNLNWDEGPYYQTKRFDRYNEIIAQMLEQGTAYKCYCSRERIETMREEQAAKGEQQK
HHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCHHH
YDGCCRDKAPRDTDEPFVVRFKNPTEGSVVFDDHVRGRIEFANSTLDDLIIARTEGTPTY
HCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCCCCEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVGVMQYRDDGYLPEALLNYLVRLGWSHGDQEVFSIDEMKQFFNLDDINKAASAFNTD
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCC
KLIWLNQHYIKESDPEYVASHLEWHMADQNIDTSNGPKLSEVVSALSERAKTLKELAASS
EEEEECHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RYFFEDFAEFDETAAKKHLRGVALEPLTLFQTKLAGLNDWTLEAIHQAIEDTATELEVGM
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GKVGMPLRVAVTGAGMSPAVDLTIFLVGKARTEQRISKAIEFVANRINS
CCCCCCEEEEEECCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA