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The map label for this gene is napF [H]

Identifier: 157961271

GI number: 157961271

Start: 1759212

End: 1759691

Strand: Reverse

Name: napF [H]

Synonym: Spea_1445

Alternate gene names: 157961271

Gene position: 1759691-1759212 (Counterclockwise)

Preceding gene: 157961272

Following gene: 157961270

Centisome position: 34.01

GC content: 48.54

Gene sequence:

>480_bases
ATGAGCAGCATTAATCAAAGCAGAAGAAGACTTTTCAACCGCAAAAAGAATGATGCTGTTCGTCCTCCTTGGGTGAAACT
GTCAATCGAGTTTACCGACGAATGCACTCGCTGCAATAAATGCATTTCAGCCTGTGAGACGCAAATCATTGTCAAGGGTG
ATGGCGGTTTTCCTGAAGTAGACTTTAAGATTGATGAGTGCACCTTCTGCCAAGAATGTGCCAATGCCTGCCCAGAAAAC
CTCTTTGAAGATACTGATACCACGCCATGGCAAGTTAAGGCCAAGATCCAAGATAGCTGTATGGCATATCAAGGGATCTG
GTGCCAAAGCTGTAAAGACTCCTGTGACTCCCGCGCAATCAGCTTCACCCTTGCTGTGGGTAAAGCGCCCCTACCTCAAA
TCGATACTGAAGCCTGTACTGGATGCGGAGCTTGCGTAGCCCCTTGTCCATCGGGCGCGATCATCATTCGCACCGCGTAA

Upstream 100 bases:

>100_bases
AAGAAAAGTAAGAATCAAAGGCAAAACTGTTTTGCTTTTATCTTGATCTCCCTAGGAGGCCGCAGGCCGCCCTCGTACCT
TCTTTTAAAAGAGTAAACAG

Downstream 100 bases:

>100_bases
AATAGCCAAAAATATGACATGTTAGCGCCAAATTTAAGTGGTTTTGTTTCCTCTAATTTCAAGGTAACATCTAAGCAACT
TAGACTGACAATGACACCAT

Product: ferredoxin-type protein NapF

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSSINQSRRRLFNRKKNDAVRPPWVKLSIEFTDECTRCNKCISACETQIIVKGDGGFPEVDFKIDECTFCQECANACPEN
LFEDTDTTPWQVKAKIQDSCMAYQGIWCQSCKDSCDSRAISFTLAVGKAPLPQIDTEACTGCGACVAPCPSGAIIIRTA

Sequences:

>Translated_159_residues
MSSINQSRRRLFNRKKNDAVRPPWVKLSIEFTDECTRCNKCISACETQIIVKGDGGFPEVDFKIDECTFCQECANACPEN
LFEDTDTTPWQVKAKIQDSCMAYQGIWCQSCKDSCDSRAISFTLAVGKAPLPQIDTEACTGCGACVAPCPSGAIIIRTA
>Mature_158_residues
SSINQSRRRLFNRKKNDAVRPPWVKLSIEFTDECTRCNKCISACETQIIVKGDGGFPEVDFKIDECTFCQECANACPENL
FEDTDTTPWQVKAKIQDSCMAYQGIWCQSCKDSCDSRAISFTLAVGKAPLPQIDTEACTGCGACVAPCPSGAIIIRTA

Specific function: Involved in electron transfer [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=154, Percent_Identity=44.8051948051948, Blast_Score=149, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17478; Mature: 17347

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
11.3 %Cys+Met (Translated Protein)
10.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
10.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSINQSRRRLFNRKKNDAVRPPWVKLSIEFTDECTRCNKCISACETQIIVKGDGGFPEV
CCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHCCEEEEECCCCCCCE
DFKIDECTFCQECANACPENLFEDTDTTPWQVKAKIQDSCMAYQGIWCQSCKDSCDSRAI
EEEECCHHHHHHHHHHCHHHHHCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHCCCCCCEE
SFTLAVGKAPLPQIDTEACTGCGACVAPCPSGAIIIRTA
EEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
SSINQSRRRLFNRKKNDAVRPPWVKLSIEFTDECTRCNKCISACETQIIVKGDGGFPEV
CCCHHHHHHHHHCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHCCEEEEECCCCCCCE
DFKIDECTFCQECANACPENLFEDTDTTPWQVKAKIQDSCMAYQGIWCQSCKDSCDSRAI
EEEECCHHHHHHHHHHCHHHHHCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHCCCCCCEE
SFTLAVGKAPLPQIDTEACTGCGACVAPCPSGAIIIRTA
EEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]