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The map label for this gene is mlaA [H]

Identifier: 157961218

GI number: 157961218

Start: 1688215

End: 1688982

Strand: Direct

Name: mlaA [H]

Synonym: Spea_1390

Alternate gene names: 157961218

Gene position: 1688215-1688982 (Clockwise)

Preceding gene: 157961215

Following gene: 157961219

Centisome position: 32.63

GC content: 44.4

Gene sequence:

>768_bases
ATGAAGTTTAAATGGATTGTGCTTTCTCTGGCGATATTTGGAATGCCTTCAATTGAAGCTGCAGAAGATACCACTACGGA
CGGTGAGCAACAGATAACGGTAACCTATAATGACCCACGAGATCCGATAGAGGGCTTTAACCGAGCCATGTGGGACTTTA
ACTATCTGTTTATGGACCGTTATTTTTACCGCCCCGTTGCCCATGGCTATAATGATTACATTCCTCATCCAGTAAAAAGT
GGGGTTAATAATTTTGTACTAAACCTCGAAGAGCCTTCAACCTTAGTTAACAATACATTACAAGGAAATTGGGGCTGGGC
TGCTAATGCTGGCGGCCGATTTACGGTCAATACCACATTAGGCCTGTTAGGGGTGATTGATGTTGCCGAGATGATGGGGA
TGACTCGTAAGCAAGATGCTTTTAACGAAGTGCTTGGTTATTATGGTGTACCGAATGGCCCGTACTTCATGGCGCCATTT
TTCGGTCCTTATGTTACTCGTGAGCTCGCGTCAGACTGGGTTGATGATCTATACTTCCCTCTGTCTGAGCTGACGTTCTG
GCAGTCAGTCTTAAAATGGGGTCTTAAAAACTTGCATACGCGAGCGTCTGCAATCGATCAGGAACGATTGGTTGATAATG
CGCTCGACCCATATACTTTTGTGAAGGATGCATACTTTCAGCACATGGACTACAAGGTATATGACGGTGACATTCCTCAA
AGTGACGACGATGAGTTACTTGATGAGTACATGCAGGAATTAGAATAG

Upstream 100 bases:

>100_bases
AAATCGTTGAGATTTATGTGTGAATATTTATACTCTAGTCAGTGAGTCGGAATGGGCAGGCAACGACTAGTACAACGAAC
TCCTTTTCAAAGAGACAATT

Downstream 100 bases:

>100_bases
CATTAGAATAAGTGGACAAATAATAATAACTGCTCCGCTTAAAGCGTAAACAAGACACAAGGACGTCGGTTTTCGCTGTT
GTCAAAGGGCAGCTTATGGC

Product: VacJ family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKFKWIVLSLAIFGMPSIEAAEDTTTDGEQQITVTYNDPRDPIEGFNRAMWDFNYLFMDRYFYRPVAHGYNDYIPHPVKS
GVNNFVLNLEEPSTLVNNTLQGNWGWAANAGGRFTVNTTLGLLGVIDVAEMMGMTRKQDAFNEVLGYYGVPNGPYFMAPF
FGPYVTRELASDWVDDLYFPLSELTFWQSVLKWGLKNLHTRASAIDQERLVDNALDPYTFVKDAYFQHMDYKVYDGDIPQ
SDDDELLDEYMQELE

Sequences:

>Translated_255_residues
MKFKWIVLSLAIFGMPSIEAAEDTTTDGEQQITVTYNDPRDPIEGFNRAMWDFNYLFMDRYFYRPVAHGYNDYIPHPVKS
GVNNFVLNLEEPSTLVNNTLQGNWGWAANAGGRFTVNTTLGLLGVIDVAEMMGMTRKQDAFNEVLGYYGVPNGPYFMAPF
FGPYVTRELASDWVDDLYFPLSELTFWQSVLKWGLKNLHTRASAIDQERLVDNALDPYTFVKDAYFQHMDYKVYDGDIPQ
SDDDELLDEYMQELE
>Mature_255_residues
MKFKWIVLSLAIFGMPSIEAAEDTTTDGEQQITVTYNDPRDPIEGFNRAMWDFNYLFMDRYFYRPVAHGYNDYIPHPVKS
GVNNFVLNLEEPSTLVNNTLQGNWGWAANAGGRFTVNTTLGLLGVIDVAEMMGMTRKQDAFNEVLGYYGVPNGPYFMAPF
FGPYVTRELASDWVDDLYFPLSELTFWQSVLKWGLKNLHTRASAIDQERLVDNALDPYTFVKDAYFQHMDYKVYDGDIPQ
SDDDELLDEYMQELE

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=246, Percent_Identity=38.2113821138211, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 29371; Mature: 29371

Theoretical pI: Translated: 4.08; Mature: 4.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKWIVLSLAIFGMPSIEAAEDTTTDGEQQITVTYNDPRDPIEGFNRAMWDFNYLFMDR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
YFYRPVAHGYNDYIPHPVKSGVNNFVLNLEEPSTLVNNTLQGNWGWAANAGGRFTVNTTL
HHHHHHHCCCCCCCCCHHHCCCCCEEEECCCCHHHHHCCCCCCCCEECCCCCEEEEEHHH
GLLGVIDVAEMMGMTRKQDAFNEVLGYYGVPNGPYFMAPFFGPYVTRELASDWVDDLYFP
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
LSELTFWQSVLKWGLKNLHTRASAIDQERLVDNALDPYTFVKDAYFQHMDYKVYDGDIPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCC
SDDDELLDEYMQELE
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MKFKWIVLSLAIFGMPSIEAAEDTTTDGEQQITVTYNDPRDPIEGFNRAMWDFNYLFMDR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
YFYRPVAHGYNDYIPHPVKSGVNNFVLNLEEPSTLVNNTLQGNWGWAANAGGRFTVNTTL
HHHHHHHCCCCCCCCCHHHCCCCCEEEECCCCHHHHHCCCCCCCCEECCCCCEEEEEHHH
GLLGVIDVAEMMGMTRKQDAFNEVLGYYGVPNGPYFMAPFFGPYVTRELASDWVDDLYFP
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
LSELTFWQSVLKWGLKNLHTRASAIDQERLVDNALDPYTFVKDAYFQHMDYKVYDGDIPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCC
SDDDELLDEYMQELE
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]