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The map label for this gene is flbD [H]

Identifier: 157961187

GI number: 157961187

Start: 1655947

End: 1657299

Strand: Direct

Name: flbD [H]

Synonym: Spea_1359

Alternate gene names: 157961187

Gene position: 1655947-1657299 (Clockwise)

Preceding gene: 157961186

Following gene: 157961188

Centisome position: 32.0

GC content: 48.26

Gene sequence:

>1353_bases
ATGTCTGAAGGTAAGTTATTACTTGTAGAAGATGATGCTTCACTGCGTGAAGCACTGCTCGATACCTTGATGTTAGCGCA
TTATGATTGTGTCGATGTCGCTTCGGCAGAAGAAGCTATCCTTTCTCTAAAAGCCAACAGATACGATATGGTGATAAGCG
ACGTGCAAATGGAGGGCGTAGGAGGCATAGGCCTGCTCAACTATATGCAGCAACATCACCCGAAAATTCCAGTATTGTTG
ATGACCGCATATGCGACCATAGATAACGCGGTAAATGCGATGAAACTTGGCGCGGTCGATTATTTGGCTAAGCCGTTTTC
GTCAGAGGTTCTACTCAATCAAGTCTCTCGTTATTTGCCAGCTAAAGTGGTTGAAGGCACGCCAATCGTTGCTGATGAAA
AGAGTATTGCACTGTTAGCATTGGCGCAGCGGGTGGCTGCATCCGATGCCTCTGTGATGATTATGGGGCCGAGCGGCAGC
GGTAAAGAGGTATTAGCGCGTTATATTCATCAGAACAGCCAACGCGTCGACCAGCCGTTTGTCGCCATTAACTGCGCGGC
AATTCCTGAAAACATGCTTGAGGCGACACTGTTTGGCTATGAGAAGGGCGCCTTTACCGGGGCTTATCAAGCCTGTCCTG
GCAAGTTTGAACAGGCGCAAGGTGGTACTTTGCTGCTTGATGAAATATCTGAGATGGAAGTCGGCTTGCAAGCAAAATTG
TTGCGGGTATTGCAAGAGCGTGAAGTGGAGCGTTTAGGCGGCCGTAAAACGATTAAGCTTAATGTGCGGGTGCTGGCCAC
CTCGAATCGCGACCTAAAAGCGATGGCATCATCGGGAGAATTTAGGGAAGACTTGTACTACCGAATTAACGTCTTCCCGC
TGACTTGGCCGTCATTAAACCAACGCCCTGCGGATATCTTGCCACTAGCAAGGCACTTGCTGCAGCGCCATGCCTTAATT
GCCAACCGTAGTGAAATCCCTGAGTTCAGTGAGTGCGCGACTCGCCGTTTATTGACTCATCGCTGGCCTGGCAATGTACG
CGAACTCGATAATGTGGTGCAAAGAGCACTTATCTTGTCAGTATCAGCTGAAGTGACTGCGGCAGATATTATAATCGACT
CACAAGAGTTAGGTTTTACCGCCGAGGTAATACCTATGGTTGAGAGTAAACCTGCCGAGCTCGATGGTTTAGGTGACGAA
TTAAAGGCGCAAGAGCATGTGATTATTTTAGAAACCTTAACTCAATGTGACGGTAGTCGTAAGCTTGTCGCCGAGAAGTT
AGGGATCAGTGCCAGAACTCTGCGTTACAAGATGGCTAAAATGCGTGAATTAGGCATACAAATACCGGCTTAG

Upstream 100 bases:

>100_bases
AGCTGTCATCCCGGTAATGGCTGCACTGCGTCATTAAAGTTTCCGTTGATCACGACCATTAATAACCATAATAACAATGA
AGTGCTTGGAGATAATCAGC

Downstream 100 bases:

>100_bases
AGCTAAGAAGGACGCCCTGCGGGCTGATGGGACCAAGAACCTAGGACGTTCGGCAGGCTCACTGCGAGGACGCTTCGCTA
CTAGGGCCTAGGAAAAGCAT

Product: Fis family two component sigma54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSEGKLLLVEDDASLREALLDTLMLAHYDCVDVASAEEAILSLKANRYDMVISDVQMEGVGGIGLLNYMQQHHPKIPVLL
MTAYATIDNAVNAMKLGAVDYLAKPFSSEVLLNQVSRYLPAKVVEGTPIVADEKSIALLALAQRVAASDASVMIMGPSGS
GKEVLARYIHQNSQRVDQPFVAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKL
LRVLQEREVERLGGRKTIKLNVRVLATSNRDLKAMASSGEFREDLYYRINVFPLTWPSLNQRPADILPLARHLLQRHALI
ANRSEIPEFSECATRRLLTHRWPGNVRELDNVVQRALILSVSAEVTAADIIIDSQELGFTAEVIPMVESKPAELDGLGDE
LKAQEHVIILETLTQCDGSRKLVAEKLGISARTLRYKMAKMRELGIQIPA

Sequences:

>Translated_450_residues
MSEGKLLLVEDDASLREALLDTLMLAHYDCVDVASAEEAILSLKANRYDMVISDVQMEGVGGIGLLNYMQQHHPKIPVLL
MTAYATIDNAVNAMKLGAVDYLAKPFSSEVLLNQVSRYLPAKVVEGTPIVADEKSIALLALAQRVAASDASVMIMGPSGS
GKEVLARYIHQNSQRVDQPFVAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKL
LRVLQEREVERLGGRKTIKLNVRVLATSNRDLKAMASSGEFREDLYYRINVFPLTWPSLNQRPADILPLARHLLQRHALI
ANRSEIPEFSECATRRLLTHRWPGNVRELDNVVQRALILSVSAEVTAADIIIDSQELGFTAEVIPMVESKPAELDGLGDE
LKAQEHVIILETLTQCDGSRKLVAEKLGISARTLRYKMAKMRELGIQIPA
>Mature_449_residues
SEGKLLLVEDDASLREALLDTLMLAHYDCVDVASAEEAILSLKANRYDMVISDVQMEGVGGIGLLNYMQQHHPKIPVLLM
TAYATIDNAVNAMKLGAVDYLAKPFSSEVLLNQVSRYLPAKVVEGTPIVADEKSIALLALAQRVAASDASVMIMGPSGSG
KEVLARYIHQNSQRVDQPFVAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKLL
RVLQEREVERLGGRKTIKLNVRVLATSNRDLKAMASSGEFREDLYYRINVFPLTWPSLNQRPADILPLARHLLQRHALIA
NRSEIPEFSECATRRLLTHRWPGNVRELDNVVQRALILSVSAEVTAADIIIDSQELGFTAEVIPMVESKPAELDGLGDEL
KAQEHVIILETLTQCDGSRKLVAEKLGISARTLRYKMAKMRELGIQIPA

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=460, Percent_Identity=40.4347826086956, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI1790437, Length=446, Percent_Identity=38.3408071748879, Blast_Score=290, Evalue=9e-80,
Organism=Escherichia coli, GI1788905, Length=374, Percent_Identity=42.5133689839572, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1790299, Length=476, Percent_Identity=34.453781512605, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87082117, Length=339, Percent_Identity=43.952802359882, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1789087, Length=328, Percent_Identity=41.1585365853659, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789233, Length=304, Percent_Identity=41.7763157894737, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI87082152, Length=245, Percent_Identity=44.0816326530612, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI87081872, Length=307, Percent_Identity=37.4592833876222, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1786524, Length=259, Percent_Identity=42.4710424710425, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787583, Length=307, Percent_Identity=35.1791530944625, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1789828, Length=260, Percent_Identity=35.3846153846154, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87081858, Length=244, Percent_Identity=29.9180327868852, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790552, Length=107, Percent_Identity=35.5140186915888, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786784, Length=132, Percent_Identity=34.8484848484849, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49504; Mature: 49373

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGKLLLVEDDASLREALLDTLMLAHYDCVDVASAEEAILSLKANRYDMVISDVQMEGV
CCCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCEEEEEEEEEECCC
GGIGLLNYMQQHHPKIPVLLMTAYATIDNAVNAMKLGAVDYLAKPFSSEVLLNQVSRYLP
CCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
AKVVEGTPIVADEKSIALLALAQRVAASDASVMIMGPSGSGKEVLARYIHQNSQRVDQPF
CHHCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCHHCCCCE
VAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKL
EEEEEHHCCHHHHHHHHHCCCCCCCCCCHHCCCCCCHHCCCCEEEEHHHHHHHHHHHHHH
LRVLQEREVERLGGRKTIKLNVRVLATSNRDLKAMASSGEFREDLYYRINVFPLTWPSLN
HHHHHHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHCCCCCCCCEEEEEEEEEECCCCCC
QRPADILPLARHLLQRHALIANRSEIPEFSECATRRLLTHRWPGNVRELDNVVQRALILS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VSAEVTAADIIIDSQELGFTAEVIPMVESKPAELDGLGDELKAQEHVIILETLTQCDGSR
CCCCEEEEEEEEECHHCCCEEEEEECCCCCCCCCCCCCHHHCCCCCEEEEEHHHHCCCCH
KLVAEKLGISARTLRYKMAKMRELGIQIPA
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEGKLLLVEDDASLREALLDTLMLAHYDCVDVASAEEAILSLKANRYDMVISDVQMEGV
CCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCEEEEEEEEEECCC
GGIGLLNYMQQHHPKIPVLLMTAYATIDNAVNAMKLGAVDYLAKPFSSEVLLNQVSRYLP
CCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
AKVVEGTPIVADEKSIALLALAQRVAASDASVMIMGPSGSGKEVLARYIHQNSQRVDQPF
CHHCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCHHCCCCE
VAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEISEMEVGLQAKL
EEEEEHHCCHHHHHHHHHCCCCCCCCCCHHCCCCCCHHCCCCEEEEHHHHHHHHHHHHHH
LRVLQEREVERLGGRKTIKLNVRVLATSNRDLKAMASSGEFREDLYYRINVFPLTWPSLN
HHHHHHHHHHHHCCCEEEEEEEEEEEECCCCHHHHHCCCCCCCCEEEEEEEEEECCCCCC
QRPADILPLARHLLQRHALIANRSEIPEFSECATRRLLTHRWPGNVRELDNVVQRALILS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VSAEVTAADIIIDSQELGFTAEVIPMVESKPAELDGLGDELKAQEHVIILETLTQCDGSR
CCCCEEEEEEEEECHHCCCEEEEEECCCCCCCCCCCCCHHHCCCCCEEEEEHHHHCCCCH
KLVAEKLGISARTLRYKMAKMRELGIQIPA
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]