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The map label for this gene is ntrC [H]

Identifier: 157961185

GI number: 157961185

Start: 1652848

End: 1654281

Strand: Direct

Name: ntrC [H]

Synonym: Spea_1357

Alternate gene names: 157961185

Gene position: 1652848-1654281 (Clockwise)

Preceding gene: 157961184

Following gene: 157961186

Centisome position: 31.94

GC content: 44.42

Gene sequence:

>1434_bases
ATGATGCAAACAGATCAACGAATTCTACTTGTCGGTAACCAGTCAGAGCGAATTAATCGCTTGTCGTGTGTATTTGAGTT
CTTAGGAGAGCAGGTCGAACTGATCGACTTCGATAAACTCGAGACGTACACTAAGCAGACGCGCTTTCGCGCGATTGTTT
TACCTTCTGAGAACCAATCGAAAGAACTTATCCAGTCTTTGACTGGTACGCTTCCTTGGCAGCCTTTTTTAATGTTAGGG
GAAAGAGGCGACATTAAAACGTCGAATATTCTCGGCTGCATCGAAGAACCCTTAAACTACCCACAATTAACTGAGTTACT
CCACTTTTGTCAGGTCTATGGTCAAGTTAAACGTCCAGAGATCCCAACTAGCGCAAATCAGACTAAATTATTTAGAAGTT
TAGTGGGTCGTAGCGAGGGTATTGCCAATGTTCGTCACCTCATTAATCAGGTGGCAGGTTCTGACGCTACAGTATTAGTA
CTTGGGCAATCAGGAACGGGTAAAGAAGTGGTTGCTCGTAATATTCACTATATTTCAGAACGTCGCAATGGACCGTTTAT
TCCGGTAAATTGCGGTGCGATTCCTCCAGAATTACTGGAGAGTGAACTTTTTGGTCATGAGAAGGGTTCATTCACCGGGG
CAATCAGTGCACGTAAAGGTCGTTTCGAACTTGCGGAAAAAGGCACCTTATTCCTCGATGAGATTGGCGATATGCCATTG
CAGATGCAAGTTAAATTATTACGTGTACTGCAAGAGCGTATGTTTGAGCGTGTTGGTGGCAGTAAGTCTATTTCAGCCGA
TGTTCGCGTTGTTGCTGCAACCCATAGAAACTTAGAAACCATGATAGAGAAGGGAGATTTTAGAGAAGATCTCTATTACC
GCCTCAATGTTTTCCCAATCGAAATGCCTGCTTTGTGCGAACGCAAAGAAGATATCCCCCTGTTACTGCAAGAATTAGTC
AGCCGTGTTTATAACGAAGGCCGCGGCCGAGTTCGTTTCACTCAACGTGCAATCGAGTCTCTTAAAGAGCACTTATGGTC
AGGTAACGTTCGTGAACTGTCTAACTTGGTTGAACGTTTAACGATTCTTTATCCGGGTGGACTGGTCGATGTCAATGACT
TACCTATCAAGTACCGTCATATCGATGTTCCTGAATACTGTGTCGAAATCAGTGAAGAGCAGCAAGAGCGTGACGCTTTA
GCCTCTATCTTCAACGATGAAGAGCCTATCGAGATCCCAGAGACGCGATTCCCGAGTGAGTTGCCACCAGAAGGGGTCAA
CTTAAAAGACTTATTGGCAGAGCTTGAGATCGATATGATCAGACAAGCACTAGATCAACAAGATAGCGTGGTTGCCCGTG
CAGCAGAAATGCTAGGCATTCGTCGTACCACCTTAGTTGAAAAAATGCGTAAGTACGGATTGAGTAAAGATTAA

Upstream 100 bases:

>100_bases
TAAATTTCAGATAACTACTGGCTGAACAATTAGCTTGAGAAAAGCAACGAACACTAATAATTAATGCGCTGAAAATATTT
GAGCCTATACGGCCTTCGGA

Downstream 100 bases:

>100_bases
AGCTAAGACGAACGCTTCGCTCACGGAAGACGGAAGACGGAATCTTCGCAGGCTCAAAGCCGAGAAGGACGCCCTTCGGG
CTGCTAGGGCGTTCGCAGGC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MMQTDQRILLVGNQSERINRLSCVFEFLGEQVELIDFDKLETYTKQTRFRAIVLPSENQSKELIQSLTGTLPWQPFLMLG
ERGDIKTSNILGCIEEPLNYPQLTELLHFCQVYGQVKRPEIPTSANQTKLFRSLVGRSEGIANVRHLINQVAGSDATVLV
LGQSGTGKEVVARNIHYISERRNGPFIPVNCGAIPPELLESELFGHEKGSFTGAISARKGRFELAEKGTLFLDEIGDMPL
QMQVKLLRVLQERMFERVGGSKSISADVRVVAATHRNLETMIEKGDFREDLYYRLNVFPIEMPALCERKEDIPLLLQELV
SRVYNEGRGRVRFTQRAIESLKEHLWSGNVRELSNLVERLTILYPGGLVDVNDLPIKYRHIDVPEYCVEISEEQQERDAL
ASIFNDEEPIEIPETRFPSELPPEGVNLKDLLAELEIDMIRQALDQQDSVVARAAEMLGIRRTTLVEKMRKYGLSKD

Sequences:

>Translated_477_residues
MMQTDQRILLVGNQSERINRLSCVFEFLGEQVELIDFDKLETYTKQTRFRAIVLPSENQSKELIQSLTGTLPWQPFLMLG
ERGDIKTSNILGCIEEPLNYPQLTELLHFCQVYGQVKRPEIPTSANQTKLFRSLVGRSEGIANVRHLINQVAGSDATVLV
LGQSGTGKEVVARNIHYISERRNGPFIPVNCGAIPPELLESELFGHEKGSFTGAISARKGRFELAEKGTLFLDEIGDMPL
QMQVKLLRVLQERMFERVGGSKSISADVRVVAATHRNLETMIEKGDFREDLYYRLNVFPIEMPALCERKEDIPLLLQELV
SRVYNEGRGRVRFTQRAIESLKEHLWSGNVRELSNLVERLTILYPGGLVDVNDLPIKYRHIDVPEYCVEISEEQQERDAL
ASIFNDEEPIEIPETRFPSELPPEGVNLKDLLAELEIDMIRQALDQQDSVVARAAEMLGIRRTTLVEKMRKYGLSKD
>Mature_477_residues
MMQTDQRILLVGNQSERINRLSCVFEFLGEQVELIDFDKLETYTKQTRFRAIVLPSENQSKELIQSLTGTLPWQPFLMLG
ERGDIKTSNILGCIEEPLNYPQLTELLHFCQVYGQVKRPEIPTSANQTKLFRSLVGRSEGIANVRHLINQVAGSDATVLV
LGQSGTGKEVVARNIHYISERRNGPFIPVNCGAIPPELLESELFGHEKGSFTGAISARKGRFELAEKGTLFLDEIGDMPL
QMQVKLLRVLQERMFERVGGSKSISADVRVVAATHRNLETMIEKGDFREDLYYRLNVFPIEMPALCERKEDIPLLLQELV
SRVYNEGRGRVRFTQRAIESLKEHLWSGNVRELSNLVERLTILYPGGLVDVNDLPIKYRHIDVPEYCVEISEEQQERDAL
ASIFNDEEPIEIPETRFPSELPPEGVNLKDLLAELEIDMIRQALDQQDSVVARAAEMLGIRRTTLVEKMRKYGLSKD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=350, Percent_Identity=43.7142857142857, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI87082117, Length=348, Percent_Identity=42.2413793103448, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1788550, Length=326, Percent_Identity=43.8650306748466, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1790437, Length=408, Percent_Identity=39.2156862745098, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1789233, Length=349, Percent_Identity=42.1203438395415, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1788905, Length=328, Percent_Identity=42.0731707317073, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1790299, Length=344, Percent_Identity=40.406976744186, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082152, Length=342, Percent_Identity=37.4269005847953, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87081872, Length=342, Percent_Identity=35.3801169590643, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786524, Length=352, Percent_Identity=36.6477272727273, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1787583, Length=355, Percent_Identity=32.6760563380282, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789828, Length=235, Percent_Identity=36.1702127659575, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI87081858, Length=329, Percent_Identity=29.483282674772, Blast_Score=142, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54253; Mature: 54253

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4 ; PS00276 CHANNEL_COLICIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQTDQRILLVGNQSERINRLSCVFEFLGEQVELIDFDKLETYTKQTRFRAIVLPSENQS
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEEEEECCCCCH
KELIQSLTGTLPWQPFLMLGERGDIKTSNILGCIEEPLNYPQLTELLHFCQVYGQVKRPE
HHHHHHHHCCCCCCHHEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
IPTSANQTKLFRSLVGRSEGIANVRHLINQVAGSDATVLVLGQSGTGKEVVARNIHYISE
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
RRNGPFIPVNCGAIPPELLESELFGHEKGSFTGAISARKGRFELAEKGTLFLDEIGDMPL
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCEEHHCCCCEEHHHHCCCCH
QMQVKLLRVLQERMFERVGGSKSISADVRVVAATHRNLETMIEKGDFREDLYYRLNVFPI
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHEEEEEEEEE
EMPALCERKEDIPLLLQELVSRVYNEGRGRVRFTQRAIESLKEHLWSGNVRELSNLVERL
CCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TILYPGGLVDVNDLPIKYRHIDVPEYCVEISEEQQERDALASIFNDEEPIEIPETRFPSE
HHCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
LPPEGVNLKDLLAELEIDMIRQALDQQDSVVARAAEMLGIRRTTLVEKMRKYGLSKD
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMQTDQRILLVGNQSERINRLSCVFEFLGEQVELIDFDKLETYTKQTRFRAIVLPSENQS
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEEEEECCCCCH
KELIQSLTGTLPWQPFLMLGERGDIKTSNILGCIEEPLNYPQLTELLHFCQVYGQVKRPE
HHHHHHHHCCCCCCHHEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
IPTSANQTKLFRSLVGRSEGIANVRHLINQVAGSDATVLVLGQSGTGKEVVARNIHYISE
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
RRNGPFIPVNCGAIPPELLESELFGHEKGSFTGAISARKGRFELAEKGTLFLDEIGDMPL
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCEEHHCCCCEEHHHHCCCCH
QMQVKLLRVLQERMFERVGGSKSISADVRVVAATHRNLETMIEKGDFREDLYYRLNVFPI
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHEEEEEEEEE
EMPALCERKEDIPLLLQELVSRVYNEGRGRVRFTQRAIESLKEHLWSGNVRELSNLVERL
CCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TILYPGGLVDVNDLPIKYRHIDVPEYCVEISEEQQERDALASIFNDEEPIEIPETRFPSE
HHCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
LPPEGVNLKDLLAELEIDMIRQALDQQDSVVARAAEMLGIRRTTLVEKMRKYGLSKD
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]