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The map label for this gene is flgJ [H]

Identifier: 157961176

GI number: 157961176

Start: 1641697

End: 1642788

Strand: Direct

Name: flgJ [H]

Synonym: Spea_1348

Alternate gene names: 157961176

Gene position: 1641697-1642788 (Clockwise)

Preceding gene: 157961175

Following gene: 157961177

Centisome position: 31.73

GC content: 46.34

Gene sequence:

>1092_bases
ATGGAGAAGTTGTCGAACGCATCGCAATTTCTGGATCTTGGAGGCTTGGACTCATTAAGATCCAGAGCTCAAAAAGATGA
AACCAGTGCATTAAAAGAAGTTGCCCAGCAATTCGAGGGGATCTTCGTGCAAATGCTGATGAAAAGCATGCGTGATGCTA
ATGCTGTGTTTGAGTCTGACAGCCCAATGAATAGCCAGTACACCAAGTTTTATGAGCAGATGCATGATCAGCAGATGTCG
CTCAACCTTTCTGGTGAAGGCATGTTGGGCTTAGCCGATCTAATGGTGCAGCAACTTGACCCCGCTAATAGTCCGATGAC
TCCAGCCTCTGTGCTCCGTGGTGATATCAACGGCGGCTCTAAGGCTGCAGCATTGACCATGGATAGACCGGATATGCTGC
AGATGCCGAGCTCACGTATTGCTAGCCCTGACACTATTACCGATCACGTTCCAAAAAATCAGAATAGTTTCGCTAGTAAT
AGCCAAGCATCAGCACCACAAGCAATTACAAGCAGTGTTCAATCACAAACATTAGATTCGGTATTGAGTGGCAAAATATT
GCCGTCGGCGGCAGTCAACGCCGATAAGTCTCAGGCAAACTTTACCAGCCAAGATGAGTTCGTGGCGCGTCTATATCCCC
ATGCCCAAAAAGCGGCTCAAACCTTAGGTACCACACCAGAAGTATTGATTGCTCAATCGGCGCTAGAAACAGGTTGGGGC
CAGAAAATGGTCAAAGGCCATCAAGGGCAGCAGAGTAATAACCTGTTTAACATTAAGGCCGACAATCGTTGGCAAGGTGA
GAAAGCCTCAGTTAGCACCCTAGAATATGAGCAAGGTATTGCCGTTAAGCAGCAAGCGAATTTCAGAGTCTATGAGGATA
TTGGTCAAAGCTTTAATGACTTTGTTTCATTCGTTTCCAATGGTGAGCGTTATCAAGACGCGATGAAACAAGCCGCTAAT
CCGCAAGCCTTTATCCGATCCTTGCAAGAGGCTGGCTACGCAACCGACCCTAAATATGCCGATAAGGTTATTCAGGTGAT
GAAAACCATTACTCGAGACTTTAAAGATGTCTTACAAGGGGTTAAGCAATGA

Upstream 100 bases:

>100_bases
TCAGACGTACTGGCGATACTCGAAGCGTTAAAGATGGCAGGCGCTTTGCATGGTGAATTGATAATTATCTAAATATTGTC
AGTTTTATTGGAAAGGTGTC

Downstream 100 bases:

>100_bases
AGATTAGTGTTGTTCAACCACTTGAAGCAGGGAGTCAGTACGCATGTCTTTAGATTTAATGAATATAGCTCGCACAGGCG
TGCTGGCCTCTCAATCGCAA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MEKLSNASQFLDLGGLDSLRSRAQKDETSALKEVAQQFEGIFVQMLMKSMRDANAVFESDSPMNSQYTKFYEQMHDQQMS
LNLSGEGMLGLADLMVQQLDPANSPMTPASVLRGDINGGSKAAALTMDRPDMLQMPSSRIASPDTITDHVPKNQNSFASN
SQASAPQAITSSVQSQTLDSVLSGKILPSAAVNADKSQANFTSQDEFVARLYPHAQKAAQTLGTTPEVLIAQSALETGWG
QKMVKGHQGQQSNNLFNIKADNRWQGEKASVSTLEYEQGIAVKQQANFRVYEDIGQSFNDFVSFVSNGERYQDAMKQAAN
PQAFIRSLQEAGYATDPKYADKVIQVMKTITRDFKDVLQGVKQ

Sequences:

>Translated_363_residues
MEKLSNASQFLDLGGLDSLRSRAQKDETSALKEVAQQFEGIFVQMLMKSMRDANAVFESDSPMNSQYTKFYEQMHDQQMS
LNLSGEGMLGLADLMVQQLDPANSPMTPASVLRGDINGGSKAAALTMDRPDMLQMPSSRIASPDTITDHVPKNQNSFASN
SQASAPQAITSSVQSQTLDSVLSGKILPSAAVNADKSQANFTSQDEFVARLYPHAQKAAQTLGTTPEVLIAQSALETGWG
QKMVKGHQGQQSNNLFNIKADNRWQGEKASVSTLEYEQGIAVKQQANFRVYEDIGQSFNDFVSFVSNGERYQDAMKQAAN
PQAFIRSLQEAGYATDPKYADKVIQVMKTITRDFKDVLQGVKQ
>Mature_363_residues
MEKLSNASQFLDLGGLDSLRSRAQKDETSALKEVAQQFEGIFVQMLMKSMRDANAVFESDSPMNSQYTKFYEQMHDQQMS
LNLSGEGMLGLADLMVQQLDPANSPMTPASVLRGDINGGSKAAALTMDRPDMLQMPSSRIASPDTITDHVPKNQNSFASN
SQASAPQAITSSVQSQTLDSVLSGKILPSAAVNADKSQANFTSQDEFVARLYPHAQKAAQTLGTTPEVLIAQSALETGWG
QKMVKGHQGQQSNNLFNIKADNRWQGEKASVSTLEYEQGIAVKQQANFRVYEDIGQSFNDFVSFVSNGERYQDAMKQAAN
PQAFIRSLQEAGYATDPKYADKVIQVMKTITRDFKDVLQGVKQ

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=348, Percent_Identity=33.6206896551724, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 39790; Mature: 39790

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSNASQFLDLGGLDSLRSRAQKDETSALKEVAQQFEGIFVQMLMKSMRDANAVFESD
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPMNSQYTKFYEQMHDQQMSLNLSGEGMLGLADLMVQQLDPANSPMTPASVLRGDINGGS
CCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCC
KAAALTMDRPDMLQMPSSRIASPDTITDHVPKNQNSFASNSQASAPQAITSSVQSQTLDS
CEEEEECCCCHHHHCCHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VLSGKILPSAAVNADKSQANFTSQDEFVARLYPHAQKAAQTLGTTPEVLIAQSALETGWG
HHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
QKMVKGHQGQQSNNLFNIKADNRWQGEKASVSTLEYEQGIAVKQQANFRVYEDIGQSFND
HHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCHHCCCCHHHHHHHHHHHH
FVSFVSNGERYQDAMKQAANPQAFIRSLQEAGYATDPKYADKVIQVMKTITRDFKDVLQG
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKQ
HCC
>Mature Secondary Structure
MEKLSNASQFLDLGGLDSLRSRAQKDETSALKEVAQQFEGIFVQMLMKSMRDANAVFESD
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPMNSQYTKFYEQMHDQQMSLNLSGEGMLGLADLMVQQLDPANSPMTPASVLRGDINGGS
CCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCC
KAAALTMDRPDMLQMPSSRIASPDTITDHVPKNQNSFASNSQASAPQAITSSVQSQTLDS
CEEEEECCCCHHHHCCHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VLSGKILPSAAVNADKSQANFTSQDEFVARLYPHAQKAAQTLGTTPEVLIAQSALETGWG
HHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
QKMVKGHQGQQSNNLFNIKADNRWQGEKASVSTLEYEQGIAVKQQANFRVYEDIGQSFND
HHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCHHCCCCHHHHHHHHHHHH
FVSFVSNGERYQDAMKQAANPQAFIRSLQEAGYATDPKYADKVIQVMKTITRDFKDVLQG
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9742694; 10952301 [H]