Click here to switch to the map view.

The map label for this gene is mutY [H]

Identifier: 157960936

GI number: 157960936

Start: 1343393

End: 1344457

Strand: Reverse

Name: mutY [H]

Synonym: Spea_1108

Alternate gene names: 157960936

Gene position: 1344457-1343393 (Counterclockwise)

Preceding gene: 157960945

Following gene: 157960935

Centisome position: 25.98

GC content: 46.85

Gene sequence:

>1065_bases
ATGAAAACTACCGCCTCTTTTTCAACCCGAATTATTACTTGGTACGACAACTTTGGTCGTAAACAGCTCCCTTGGCAAAT
CGCTAAAACCCCATACAAAGTGTGGATTTCAGAGATCATGCTGCAGCAGACTCAAGTTGCCACCGTTATCCCCTATTTCG
AAAAATTCATTGCTCGATTTCCAGATATCGATACCTTGGCTAGTGCCGAGCAAGATGAAGTGCTGCACTATTGGACTGGG
CTTGGGTATTACGCACGCGCACGTAACCTGCATAAAGCAGCCCAAACAATGCAATCACAGTTTAGTGGTGAGTTTCCAAC
CGATTTCGATGACGTTTTAGCGCTGCCCGGCATTGGCCGCTCCACCGCTGGAGCGGTACTGTCTTTATCACTGGGGCTTA
ATTTCCCCATTCTCGACGGTAACGTTAAGCGTGTTTTGGCTCGCCACGGCGCTATCGAAGGTTGGCCTGGTAAAAAACCT
GTAGAGCAACAACTTTGGCTATTAACTGAAAACCTAACGCCAGCAAAAGATATCCAAAAATATAACCAGGCCATGATGGA
TATAGGTGCGACAGTATGCACCCGCTCAAAACCCAACTGCGCTCAATGCCCGGTCGCCATTGACTGTAAGGCTCAGTTAA
GTGGTCGTCAGAGCGAATTCCCTGGTAAAAAACCTAAGAAAACCATTCCAGAAAAATCTGCTTGGCTGCTAGTCATTGAA
GAAAATCAGCAAGTGCAGTTAGAAAAGCGTCCTCCGGCGGGTATTTGGGGCGGGCTCTGGTGCTTCCCGCAGTTTAGTCA
GCGCCAAGAATTAGATACTTATATAAAAGACAAACAGCTAAAGGTGATCTCAGAGCTTGAACTAACAGGATTTAGACACA
CCTTTAGCCACTTTCACCTCGATGTCCAGCCGGTACTCATCCGAGCAACAGGGCATAGCGATAACCAGATCATGGAACAA
ACTTCCACGGTCTGGTATAACTTAACTCATCCACCAAAAGTTGGTTTAGCCTCGGCCACCGAGCGAATTTTGGCCGACTT
AGGTTCTGTATTAAATAAGGAGTAA

Upstream 100 bases:

>100_bases
TCAAACACTGCACGTATCCAATAACAATTTGCATCATTTCCTGCTTAATAAGCACAAGTGTCGGCTTGTTAATCGCCAAG
GCTAAAGTTACACTGCGCCG

Downstream 100 bases:

>100_bases
TCATGGCTCGCACAGTAAACTGCGTGTATTTAAACAAAGAAGCAGAAGGCCTAGGTTTTCAGCTCTACCCTGGCGATTTG
GGAAAGCGTATTTTCGACAA

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKTTASFSTRIITWYDNFGRKQLPWQIAKTPYKVWISEIMLQQTQVATVIPYFEKFIARFPDIDTLASAEQDEVLHYWTG
LGYYARARNLHKAAQTMQSQFSGEFPTDFDDVLALPGIGRSTAGAVLSLSLGLNFPILDGNVKRVLARHGAIEGWPGKKP
VEQQLWLLTENLTPAKDIQKYNQAMMDIGATVCTRSKPNCAQCPVAIDCKAQLSGRQSEFPGKKPKKTIPEKSAWLLVIE
ENQQVQLEKRPPAGIWGGLWCFPQFSQRQELDTYIKDKQLKVISELELTGFRHTFSHFHLDVQPVLIRATGHSDNQIMEQ
TSTVWYNLTHPPKVGLASATERILADLGSVLNKE

Sequences:

>Translated_354_residues
MKTTASFSTRIITWYDNFGRKQLPWQIAKTPYKVWISEIMLQQTQVATVIPYFEKFIARFPDIDTLASAEQDEVLHYWTG
LGYYARARNLHKAAQTMQSQFSGEFPTDFDDVLALPGIGRSTAGAVLSLSLGLNFPILDGNVKRVLARHGAIEGWPGKKP
VEQQLWLLTENLTPAKDIQKYNQAMMDIGATVCTRSKPNCAQCPVAIDCKAQLSGRQSEFPGKKPKKTIPEKSAWLLVIE
ENQQVQLEKRPPAGIWGGLWCFPQFSQRQELDTYIKDKQLKVISELELTGFRHTFSHFHLDVQPVLIRATGHSDNQIMEQ
TSTVWYNLTHPPKVGLASATERILADLGSVLNKE
>Mature_354_residues
MKTTASFSTRIITWYDNFGRKQLPWQIAKTPYKVWISEIMLQQTQVATVIPYFEKFIARFPDIDTLASAEQDEVLHYWTG
LGYYARARNLHKAAQTMQSQFSGEFPTDFDDVLALPGIGRSTAGAVLSLSLGLNFPILDGNVKRVLARHGAIEGWPGKKP
VEQQLWLLTENLTPAKDIQKYNQAMMDIGATVCTRSKPNCAQCPVAIDCKAQLSGRQSEFPGKKPKKTIPEKSAWLLVIE
ENQQVQLEKRPPAGIWGGLWCFPQFSQRQELDTYIKDKQLKVISELELTGFRHTFSHFHLDVQPVLIRATGHSDNQIMEQ
TSTVWYNLTHPPKVGLASATERILADLGSVLNKE

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI190358497, Length=361, Percent_Identity=33.7950138504155, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI115298650, Length=361, Percent_Identity=33.7950138504155, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI115298648, Length=361, Percent_Identity=33.7950138504155, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI115298654, Length=361, Percent_Identity=33.7950138504155, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI115298652, Length=361, Percent_Identity=33.7950138504155, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI6912520, Length=361, Percent_Identity=33.7950138504155, Blast_Score=188, Evalue=7e-48,
Organism=Escherichia coli, GI1789331, Length=344, Percent_Identity=56.6860465116279, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 39960; Mature: 39960

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTASFSTRIITWYDNFGRKQLPWQIAKTPYKVWISEIMLQQTQVATVIPYFEKFIARF
CCCCCCCCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDIDTLASAEQDEVLHYWTGLGYYARARNLHKAAQTMQSQFSGEFPTDFDDVLALPGIGR
CCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
STAGAVLSLSLGLNFPILDGNVKRVLARHGAIEGWPGKKPVEQQLWLLTENLTPAKDIQK
CCCCCEEEEECCCCCEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
YNQAMMDIGATVCTRSKPNCAQCPVAIDCKAQLSGRQSEFPGKKPKKTIPEKSAWLLVIE
HHHHHHHHHHHHHCCCCCCCCCCCEEEECHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
ENQQVQLEKRPPAGIWGGLWCFPQFSQRQELDTYIKDKQLKVISELELTGFRHTFSHFHL
CCCEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
DVQPVLIRATGHSDNQIMEQTSTVWYNLTHPPKVGLASATERILADLGSVLNKE
EEEEEEEEECCCCCHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTTASFSTRIITWYDNFGRKQLPWQIAKTPYKVWISEIMLQQTQVATVIPYFEKFIARF
CCCCCCCCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDIDTLASAEQDEVLHYWTGLGYYARARNLHKAAQTMQSQFSGEFPTDFDDVLALPGIGR
CCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
STAGAVLSLSLGLNFPILDGNVKRVLARHGAIEGWPGKKPVEQQLWLLTENLTPAKDIQK
CCCCCEEEEECCCCCEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
YNQAMMDIGATVCTRSKPNCAQCPVAIDCKAQLSGRQSEFPGKKPKKTIPEKSAWLLVIE
HHHHHHHHHHHHHCCCCCCCCCCCEEEECHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
ENQQVQLEKRPPAGIWGGLWCFPQFSQRQELDTYIKDKQLKVISELELTGFRHTFSHFHL
CCCEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
DVQPVLIRATGHSDNQIMEQTSTVWYNLTHPPKVGLASATERILADLGSVLNKE
EEEEEEEEECCCCCHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]