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The map label for this gene is purR [H]

Identifier: 157960924

GI number: 157960924

Start: 1323979

End: 1324995

Strand: Reverse

Name: purR [H]

Synonym: Spea_1096

Alternate gene names: 157960924

Gene position: 1324995-1323979 (Counterclockwise)

Preceding gene: 157960927

Following gene: 157960922

Centisome position: 25.61

GC content: 43.07

Gene sequence:

>1017_bases
ATGAAAGTCACCATAAATGATGTTGCCAAACATGCTGGCGTATCGATTAAAACAGTGTCACGGGTTACCAATAATGAGCC
ATCGGTAAAGCAGGCCACGATAGATAAAGTCAATGCAGCTATTAACGAGCTGAACTATCAGCCTAATTTAGCTGCAAGAA
ACTTAGCTGGCACTAAGTCCTATGTAATTGGTTTTATTTACGATAATCCAAATGCTTATTACATCATCGATATGCAGAAC
GGTATCCTGTCTGCATGCAAAGATCTGGGCTACGAATTATTAATTCACCCTTGTAACTCAAGAGCCGAGAACATCTGTGA
AGAGCTAACCAAGTTAGTTAAGCATAACCGTTTATCAGGCTTGGTGCTTACACCACCATTATCAGAAGATCCGGTGATCT
TGGCCGCCCTTGATAATATCGACGCGAATTATGTGCGCATTATCGCGGGCGAGAAAGCCGACAAAGGCTCTGGATTAGCA
GTTTTGGTCAATGACAGATCCGGTGCGATATCCATAACTCAGCATCTTATCGACTTGGGGCATCGAGATATCGCTTTCTT
AAGTGGCGATCATGAACATGAATCAACTAAAGAGCGTTTAGCTGGATTTAAACAAGCACTAGAAGATAACAAGATCTCTG
TTAACCAAGACCATATCATCGATGGTAAATACTCTTTCGAGTCAGGGGTTCAAGGTGCAAAGCAATTATTGAATCAAAGC
GTTAGACCAACTGCCATTGTCGCTTGTAACGATGAAATCGCTGCAGGTGCTTTGTTTGCAGCACGATTAGAGGGAGTGGA
TATTCCTGCTGAGATCTCAATTGTAGGCTTCGAGGATAGTCCATTTTCACGCCAAACATGGCCTAAACTTACGACCGTGC
ATCAGCCCAATGAGGAGATCGCGCATATTGCGACTGAATTATTGATAGCGAAACGTAGAGAGGAAAAATCAGACTTGGCT
AAAATATTCCAGCCGGAGCCAGTGATTAGAGACTCTTCTGCCAAGCCAAGAGCTTAA

Upstream 100 bases:

>100_bases
TAAATTTCCGGACAAATTGCACCTTTGCCACGCTGTTTGTTAATCTTGTGTTACCATCTTTTGGCAGCACAAAGTGTGTA
ACTAACGAGAATATTGCTTA

Downstream 100 bases:

>100_bases
TTAGATTTAACTCAACTTCTTGTACTGCTTCTAGTACAACTCTAACTTTTTCGCATTATTTGTCATAACTGATAATGCGA
AAAAGTCGTTAATCGCTATA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQATIDKVNAAINELNYQPNLAARNLAGTKSYVIGFIYDNPNAYYIIDMQN
GILSACKDLGYELLIHPCNSRAENICEELTKLVKHNRLSGLVLTPPLSEDPVILAALDNIDANYVRIIAGEKADKGSGLA
VLVNDRSGAISITQHLIDLGHRDIAFLSGDHEHESTKERLAGFKQALEDNKISVNQDHIIDGKYSFESGVQGAKQLLNQS
VRPTAIVACNDEIAAGALFAARLEGVDIPAEISIVGFEDSPFSRQTWPKLTTVHQPNEEIAHIATELLIAKRREEKSDLA
KIFQPEPVIRDSSAKPRA

Sequences:

>Translated_338_residues
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQATIDKVNAAINELNYQPNLAARNLAGTKSYVIGFIYDNPNAYYIIDMQN
GILSACKDLGYELLIHPCNSRAENICEELTKLVKHNRLSGLVLTPPLSEDPVILAALDNIDANYVRIIAGEKADKGSGLA
VLVNDRSGAISITQHLIDLGHRDIAFLSGDHEHESTKERLAGFKQALEDNKISVNQDHIIDGKYSFESGVQGAKQLLNQS
VRPTAIVACNDEIAAGALFAARLEGVDIPAEISIVGFEDSPFSRQTWPKLTTVHQPNEEIAHIATELLIAKRREEKSDLA
KIFQPEPVIRDSSAKPRA
>Mature_338_residues
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQATIDKVNAAINELNYQPNLAARNLAGTKSYVIGFIYDNPNAYYIIDMQN
GILSACKDLGYELLIHPCNSRAENICEELTKLVKHNRLSGLVLTPPLSEDPVILAALDNIDANYVRIIAGEKADKGSGLA
VLVNDRSGAISITQHLIDLGHRDIAFLSGDHEHESTKERLAGFKQALEDNKISVNQDHIIDGKYSFESGVQGAKQLLNQS
VRPTAIVACNDEIAAGALFAARLEGVDIPAEISIVGFEDSPFSRQTWPKLTTVHQPNEEIAHIATELLIAKRREEKSDLA
KIFQPEPVIRDSSAKPRA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=319, Percent_Identity=32.2884012539185, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789068, Length=344, Percent_Identity=32.5581395348837, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1786540, Length=295, Percent_Identity=33.5593220338983, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789202, Length=339, Percent_Identity=30.0884955752212, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=31.2302839116719, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1788474, Length=313, Percent_Identity=34.185303514377, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=28.7009063444109, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=29.0625, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=27.6527331189711, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789456, Length=332, Percent_Identity=28.3132530120482, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787906, Length=354, Percent_Identity=26.271186440678, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1790715, Length=314, Percent_Identity=24.8407643312102, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790689, Length=314, Percent_Identity=24.8407643312102, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786268, Length=336, Percent_Identity=22.6190476190476, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36917; Mature: 36917

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQATIDKVNAAINELNYQPNLAARNLAGTKS
CEEEHHHHHHHCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCE
YVIGFIYDNPNAYYIIDMQNGILSACKDLGYELLIHPCNSRAENICEELTKLVKHNRLSG
EEEEEEEECCCEEEEEECCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
LVLTPPLSEDPVILAALDNIDANYVRIIAGEKADKGSGLAVLVNDRSGAISITQHLIDLG
EEECCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCC
HRDIAFLSGDHEHESTKERLAGFKQALEDNKISVNQDHIIDGKYSFESGVQGAKQLLNQS
CCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEECCCCEEECCCCCHHHHHHHHHHHHCCC
VRPTAIVACNDEIAAGALFAARLEGVDIPAEISIVGFEDSPFSRQTWPKLTTVHQPNEEI
CCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
AHIATELLIAKRREEKSDLAKIFQPEPVIRDSSAKPRA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQATIDKVNAAINELNYQPNLAARNLAGTKS
CEEEHHHHHHHCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCE
YVIGFIYDNPNAYYIIDMQNGILSACKDLGYELLIHPCNSRAENICEELTKLVKHNRLSG
EEEEEEEECCCEEEEEECCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
LVLTPPLSEDPVILAALDNIDANYVRIIAGEKADKGSGLAVLVNDRSGAISITQHLIDLG
EEECCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCC
HRDIAFLSGDHEHESTKERLAGFKQALEDNKISVNQDHIIDGKYSFESGVQGAKQLLNQS
CCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEECCCCEEECCCCCHHHHHHHHHHHHCCC
VRPTAIVACNDEIAAGALFAARLEGVDIPAEISIVGFEDSPFSRQTWPKLTTVHQPNEEI
CCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
AHIATELLIAKRREEKSDLAKIFQPEPVIRDSSAKPRA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA