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The map label for this gene is nadB [H]

Identifier: 157960858

GI number: 157960858

Start: 1252672

End: 1254282

Strand: Reverse

Name: nadB [H]

Synonym: Spea_1030

Alternate gene names: 157960858

Gene position: 1254282-1252672 (Counterclockwise)

Preceding gene: 157960870

Following gene: 157960848

Centisome position: 24.24

GC content: 48.23

Gene sequence:

>1611_bases
ATGAAACAAGTAGTTGAACACCAATCTGACGTTTTGGTTATCGGCAGTGGTGCTGCCGGTCTCACTTTAGCTTTGCATCT
TGCTGAAAAATCAAAAGTTATTTTGCTTTCCAAAGGACCACTAAGTGAAGGCTCGACCTATTATGCTCAGGGCGGTATTG
CCTCTGTCTTCGATGAAGATGACACCATTGAGTCCCACGTTGCCGACACATTAGTTGCCGGAGCAGGCCTTTGCGACGAG
CCTGTTGTCAAATTTACTGCCGAAAATGCCAAGGCTGCCATGCAATGGTTAATCAATTGTGGCGTAGCCTTCGATAAAGA
AGATGTACCAGCCGGTAGTACTGCCGAAGCCCCTTATCACTTAACCCGTGAAGGCGGTCATAGTCACCGACGCATTCTAC
ATGCCGCCGATGCAACAGGCAAAGAGGTGCAGGTCACCTTGCAAGACCAAGCCCGCAATCATCCCAACATTCAAGTGTTA
GAGCGTTATAACGCCATCGACTTAATTACCACTCGTAAATTAAATCGTCCGGGTAACCGCGTTCAAGGTGCCTATATTTG
GAATAGAGATGAAGAGCACGTTGAAACCGTTAGGGCACGTTTCGTTGCCTTAGCAACAGGTGGTAGTTCAAAGGTTTATC
AATATACCTCTAATCCTGACATCGCATCTGGTGATGGTATTGCAATGGCGTGGCGCTCAGGTTGTCGTATTGCCAATATG
GAGTTCAACCAATTCCATCCAACCTGTCTTTATCACGCCGATGCCAAAAACTTTTTGCTTACCGAAGCCCTTCGCGGTGA
AGGCGCGTTTTTACGTCGTCCAGATGGCACCCGTTTTATGCCAGAGTTTGATGAGCGAGCCGAACTTGCACCGCGCGACA
TCGTCGCCCGCGCAATCGATTATGAAATGAAGCGCCTAGGCTCGGATTGTGTCTATCTAGATATTACCCATAAGCCAGCA
GACTTTATCATCAAGCACTTTCCAACTATCCATAAGCGCTGTTTAGAGCTCGGCATAGATATAACAACTGACCCTATACC
AGTCGTACCTGCGGCGCACTATACCTGTGGTGGGGTCATGACAGATTTACACGGCCAAACCGATCTCAACGGCTTATACG
CCATAGGTGAAGTGGCTTATACGGGTCTGCACGGTGCTAATCGCTTAGCCAGTAATTCGCTGCTTGAATGTTTAGTCTTT
GCTCGAGCTGCCGCCGAAGACATCGAGAGTCAGCTAGGCAAGATCCCAATGCCAAGCTCTCTGCCCGCATGGGATGAGAG
CCAAGTCTCTAACTCAGATGAAGAAGTGGTGATTGCCCATAACTGGCATGAACTGCGTCTATTTATGTGGGATTACGTCG
GTATTGTACGCTCCGATAAACGCCTCGAGCGCGCGCTGCGACGCTGCGTGATGTTACAGCAAGAGATCCAAGAGTATTAC
AGTAACTTTAGAGTCAGTAATAATCTATTGGAGCTACGTAACCTAGTGCAAGTTGCCGAGCTAATCATTCGCTGCGCCAT
GGAGCGTAAAGAGAGCCGAGGACTGCATTACAACATCGATCATCCAAACAAGGATGAATCACCAAAGCCAACCATATTAC
AACCTGATTAG

Upstream 100 bases:

>100_bases
TTATGGAAGCAATGATTTTTTATATGCGACCTATGAAAGTATCCATATTCGGCGTCATGGTTTAACATAGTCGGACTCCC
AGATAACTCTATGATACCTG

Downstream 100 bases:

>100_bases
CTACAGCCAGATTAGCCCTTTGGTTAACAACTGTTTTTAACGAGAATAGTTAGTCTTTCACCTCTGACTAGCTAACTGCA
ATAGACGACACAGATGTCGA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDEDDTIESHVADTLVAGAGLCDE
PVVKFTAENAKAAMQWLINCGVAFDKEDVPAGSTAEAPYHLTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIQVL
ERYNAIDLITTRKLNRPGNRVQGAYIWNRDEEHVETVRARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRIANM
EFNQFHPTCLYHADAKNFLLTEALRGEGAFLRRPDGTRFMPEFDERAELAPRDIVARAIDYEMKRLGSDCVYLDITHKPA
DFIIKHFPTIHKRCLELGIDITTDPIPVVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAAAEDIESQLGKIPMPSSLPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDESPKPTILQPD

Sequences:

>Translated_536_residues
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDEDDTIESHVADTLVAGAGLCDE
PVVKFTAENAKAAMQWLINCGVAFDKEDVPAGSTAEAPYHLTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIQVL
ERYNAIDLITTRKLNRPGNRVQGAYIWNRDEEHVETVRARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRIANM
EFNQFHPTCLYHADAKNFLLTEALRGEGAFLRRPDGTRFMPEFDERAELAPRDIVARAIDYEMKRLGSDCVYLDITHKPA
DFIIKHFPTIHKRCLELGIDITTDPIPVVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAAAEDIESQLGKIPMPSSLPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDESPKPTILQPD
>Mature_536_residues
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDEDDTIESHVADTLVAGAGLCDE
PVVKFTAENAKAAMQWLINCGVAFDKEDVPAGSTAEAPYHLTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIQVL
ERYNAIDLITTRKLNRPGNRVQGAYIWNRDEEHVETVRARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRIANM
EFNQFHPTCLYHADAKNFLLTEALRGEGAFLRRPDGTRFMPEFDERAELAPRDIVARAIDYEMKRLGSDCVYLDITHKPA
DFIIKHFPTIHKRCLELGIDITTDPIPVVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAAAEDIESQLGKIPMPSSLPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDESPKPTILQPD

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=530, Percent_Identity=31.8867924528302, Blast_Score=195, Evalue=7e-50,
Organism=Escherichia coli, GI1788928, Length=536, Percent_Identity=70.5223880597015, Blast_Score=800, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=528, Percent_Identity=31.4393939393939, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1786942, Length=565, Percent_Identity=30.7964601769912, Blast_Score=184, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=563, Percent_Identity=31.0834813499112, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=576, Percent_Identity=29.5138888888889, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI71986328, Length=464, Percent_Identity=28.8793103448276, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322701, Length=561, Percent_Identity=30.8377896613191, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6322416, Length=563, Percent_Identity=30.1953818827709, Blast_Score=200, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6320788, Length=486, Percent_Identity=27.3662551440329, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17137288, Length=578, Percent_Identity=31.1418685121107, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24655642, Length=578, Percent_Identity=31.1418685121107, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24655647, Length=578, Percent_Identity=31.1418685121107, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24663005, Length=560, Percent_Identity=30.8928571428571, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59637; Mature: 59637

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDED
CCCHHCCCCCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCCCEEEECCCCCEECCCC
DTIESHVADTLVAGAGLCDEPVVKFTAENAKAAMQWLINCGVAFDKEDVPAGSTAEAPYH
CHHHHHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIQVLERYNAIDLITTRKLNRPGNR
EECCCCCHHHHEEEECCCCCCEEEEEEHHHCCCCCCEEEEECCCCEEEEEECCCCCCCCC
VQGAYIWNRDEEHVETVRARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRIANM
EEEEEEECCCHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEEEC
EFNQFHPTCLYHADAKNFLLTEALRGEGAFLRRPDGTRFMPEFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCHHHHHHHHHH
YEMKRLGSDCVYLDITHKPADFIIKHFPTIHKRCLELGIDITTDPIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
TDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAAAEDIESQLGKIPMPSS
CCCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
CCCCCCHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDESPKPTILQPD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQVVEHQSDVLVIGSGAAGLTLALHLAEKSKVILLSKGPLSEGSTYYAQGGIASVFDED
CCCHHCCCCCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCCCEEEECCCCCEECCCC
DTIESHVADTLVAGAGLCDEPVVKFTAENAKAAMQWLINCGVAFDKEDVPAGSTAEAPYH
CHHHHHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQVTLQDQARNHPNIQVLERYNAIDLITTRKLNRPGNR
EECCCCCHHHHEEEECCCCCCEEEEEEHHHCCCCCCEEEEECCCCEEEEEECCCCCCCCC
VQGAYIWNRDEEHVETVRARFVALATGGSSKVYQYTSNPDIASGDGIAMAWRSGCRIANM
EEEEEEECCCHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEEEC
EFNQFHPTCLYHADAKNFLLTEALRGEGAFLRRPDGTRFMPEFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCHHHHHHHHHH
YEMKRLGSDCVYLDITHKPADFIIKHFPTIHKRCLELGIDITTDPIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
TDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAAAEDIESQLGKIPMPSS
CCCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LPAWDESQVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCVMLQQEIQEYY
CCCCCCHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHYNIDHPNKDESPKPTILQPD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]