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The map label for this gene is gltD [H]

Identifier: 157960840

GI number: 157960840

Start: 1230944

End: 1232353

Strand: Reverse

Name: gltD [H]

Synonym: Spea_1012

Alternate gene names: 157960840

Gene position: 1232353-1230944 (Counterclockwise)

Preceding gene: 157960841

Following gene: 157960839

Centisome position: 23.82

GC content: 50.92

Gene sequence:

>1410_bases
ATGAGCAATGATTTTCAGTTTCTTGAAGTGGGTCGTAAAGACCCAACTAAACACGCCGCCAGACAGCGTGCCACACAATT
TATTGAGATTTATCAGCCTTTTGCCCAACCTCAGGTAAAAGAGCAAGCCGATCGCTGCCTAGACTGTGGTAATCCTTATT
GTGAATGGAAATGCCCGCTGCATAATTACATTCCCAATTGGCTGCTATTAGCTAAGCAAGGGCGCATCATGGAAGCGGCA
GAGTTGGTCCACGAAACCAATACTTTGCCAGAGATCTGCGGCAGGGTATGCCCGCAAGATAGACTGTGCGAAGGCGCTTG
TACCCTAAATGAAGAGTTCGGAGCCGTTACCATAGGTAATGTGGAAAAATACATTACCGATACGGCGATCTCACAGGGCT
GGCGCCCTGATATGTCTAAGGTGACACAGCGTAAAGAACGGGTGGCCATCATTGGCGCGGGCCCTGCGGGACTTGGCTGC
GCCGATATCTTGGCCCGCAACGGCATCAAAGCCGTGGTCTATGACAAGAACCCACAGATAGGTGGCCTGCTCACCTACGG
TATTCCGTCATTTAAACTCGATAAAGAGGTGATGCAAATTCGCCGTAACGTGCTTGAAGGCATGGGCATCGAGTTTAAGC
TCGGCGTCACCGTCGGCAAGGATATCGACTTTAAGCAATTGATTGAAGAGTACGATGCGGTATTCCTCGGGATGGGTACC
TACACCGCCATGAAGGCGAACCTTATGGGAGAAGATGCTGATGGCGTACATCAGGCACTACCCTACCTTATCGGTAACAC
CCATAACGTTATGGGCACCGAGTGCCTTGAAAATCCTTACCTCAGTCTTAAAGGCAAGCGTGTAGTGGTGTTAGGTGGAG
GGGATACCGCTATGGACTGTGTTCGCACCGCGGTACGTCAAGGTGCCACTCAAGTCATTTGTGCCTATCGCCGAGATGAA
CAGAACATGCCGGGTTCACGCCGTGAAGTGCAGAATGCCCGTGAAGAGGGAGTCGAGTTCTTATTTAACCGCCAGCCGAC
GGCAATCAAAACTGTCGATGGCAAAGTGGTTGGTATCGAATGTATCGAAACTCAATTGGGAGAGGCCGATGAAAGTGGTC
GTCGCCGCCCTGAGCCAATAGCGGGTAGTGAGCAGGTTTTAGATACCGATGCGCTTATCATCGCCTTCGGCTTTCAGCCA
AGTCCTGCAACTTGGTTAACGGATCACAATATTGAGCTCAATGAGTGGGGCTTAGTCAAAGCGCCAAAGCTTGCCGATAA
TCCATTTCAAACCAGTAACCCTAAGGTGTTTGCTGGCGGCGATATGGTGCGCGGCTCGGACCTAGTCGTCACAGCCATTG
CCGAAGGACGTGATGCCGCCATGGGCATTTTAAATTACTTAGAGGACTAA

Upstream 100 bases:

>100_bases
TTGCTTCATTTTGGTCAAACCTAAAAATGTTGAACTAAGCGATCTATTGAAGTTAGAACAATCTGAACCTGCACTAGCAG
TAATAGCGGGGTAACACAAA

Downstream 100 bases:

>100_bases
TTTAATCTAATAAGTCTACGACAAAGTTGCTTGTGTTTGCGCGCCCCAATGGGGCGCCTTTTTTTGTCTGTTATTTCCAA
ACACCTATGCGTTAGCAAGA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSNDFQFLEVGRKDPTKHAARQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPLHNYIPNWLLLAKQGRIMEAA
ELVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGNVEKYITDTAISQGWRPDMSKVTQRKERVAIIGAGPAGLGC
ADILARNGIKAVVYDKNPQIGGLLTYGIPSFKLDKEVMQIRRNVLEGMGIEFKLGVTVGKDIDFKQLIEEYDAVFLGMGT
YTAMKANLMGEDADGVHQALPYLIGNTHNVMGTECLENPYLSLKGKRVVVLGGGDTAMDCVRTAVRQGATQVICAYRRDE
QNMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIECIETQLGEADESGRRRPEPIAGSEQVLDTDALIIAFGFQP
SPATWLTDHNIELNEWGLVKAPKLADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED

Sequences:

>Translated_469_residues
MSNDFQFLEVGRKDPTKHAARQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPLHNYIPNWLLLAKQGRIMEAA
ELVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGNVEKYITDTAISQGWRPDMSKVTQRKERVAIIGAGPAGLGC
ADILARNGIKAVVYDKNPQIGGLLTYGIPSFKLDKEVMQIRRNVLEGMGIEFKLGVTVGKDIDFKQLIEEYDAVFLGMGT
YTAMKANLMGEDADGVHQALPYLIGNTHNVMGTECLENPYLSLKGKRVVVLGGGDTAMDCVRTAVRQGATQVICAYRRDE
QNMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIECIETQLGEADESGRRRPEPIAGSEQVLDTDALIIAFGFQP
SPATWLTDHNIELNEWGLVKAPKLADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED
>Mature_468_residues
SNDFQFLEVGRKDPTKHAARQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPLHNYIPNWLLLAKQGRIMEAAE
LVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGNVEKYITDTAISQGWRPDMSKVTQRKERVAIIGAGPAGLGCA
DILARNGIKAVVYDKNPQIGGLLTYGIPSFKLDKEVMQIRRNVLEGMGIEFKLGVTVGKDIDFKQLIEEYDAVFLGMGTY
TAMKANLMGEDADGVHQALPYLIGNTHNVMGTECLENPYLSLKGKRVVVLGGGDTAMDCVRTAVRQGATQVICAYRRDEQ
NMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIECIETQLGEADESGRRRPEPIAGSEQVLDTDALIIAFGFQPS
PATWLTDHNIELNEWGLVKAPKLADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=446, Percent_Identity=30.2690582959641, Blast_Score=179, Evalue=7e-45,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=65.3846153846154, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=460, Percent_Identity=59.3478260869565, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI87082180, Length=460, Percent_Identity=56.304347826087, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1788468, Length=445, Percent_Identity=28.5393258426966, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789243, Length=426, Percent_Identity=32.1596244131455, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17570289, Length=492, Percent_Identity=33.5365853658537, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71984108, Length=447, Percent_Identity=31.5436241610738, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17543860, Length=212, Percent_Identity=28.7735849056604, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=484, Percent_Identity=32.6446280991736, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI28574881, Length=484, Percent_Identity=36.9834710743802, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24665539, Length=484, Percent_Identity=36.9834710743802, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24665547, Length=484, Percent_Identity=36.9834710743802, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24665543, Length=484, Percent_Identity=36.9834710743802, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24640763, Length=442, Percent_Identity=27.6018099547511, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI18858217, Length=442, Percent_Identity=27.6018099547511, Blast_Score=161, Evalue=7e-40,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51676; Mature: 51544

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFQFLEVGRKDPTKHAARQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPL
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLLLAKQGRIMEAAELVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGN
HHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECC
VEKYITDTAISQGWRPDMSKVTQRKERVAIIGAGPAGLGCADILARNGIKAVVYDKNPQI
HHHHHHHHHHHCCCCCCHHHHHHHHCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCC
GGLLTYGIPSFKLDKEVMQIRRNVLEGMGIEFKLGVTVGKDIDFKQLIEEYDAVFLGMGT
CCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCEEEEECCH
YTAMKANLMGEDADGVHQALPYLIGNTHNVMGTECLENPYLSLKGKRVVVLGGGDTAMDC
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCEEECCCEEEEECCCCHHHHH
VRTAVRQGATQVICAYRRDEQNMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIE
HHHHHHCCCHHEEHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEE
CIETQLGEADESGRRRPEPIAGSEQVLDTDALIIAFGFQPSPATWLTDHNIELNEWGLVK
EHHHHCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCCCCEEECCCEEECCCCCEE
APKLADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED
CCCCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SNDFQFLEVGRKDPTKHAARQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPL
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLLLAKQGRIMEAAELVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGN
HHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECC
VEKYITDTAISQGWRPDMSKVTQRKERVAIIGAGPAGLGCADILARNGIKAVVYDKNPQI
HHHHHHHHHHHCCCCCCHHHHHHHHCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCC
GGLLTYGIPSFKLDKEVMQIRRNVLEGMGIEFKLGVTVGKDIDFKQLIEEYDAVFLGMGT
CCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCEEEEECCH
YTAMKANLMGEDADGVHQALPYLIGNTHNVMGTECLENPYLSLKGKRVVVLGGGDTAMDC
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCEEECCCEEEEECCCCHHHHH
VRTAVRQGATQVICAYRRDEQNMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIE
HHHHHHCCCHHEEHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEE
CIETQLGEADESGRRRPEPIAGSEQVLDTDALIIAFGFQPSPATWLTDHNIELNEWGLVK
EHHHHCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCCCCEEECCCEEECCCCCEE
APKLADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED
CCCCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]