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The map label for this gene is amtB [C]

Identifier: 157960662

GI number: 157960662

Start: 1033956

End: 1035206

Strand: Direct

Name: amtB [C]

Synonym: Spea_0834

Alternate gene names: 157960662

Gene position: 1033956-1035206 (Clockwise)

Preceding gene: 157960661

Following gene: 157960663

Centisome position: 19.98

GC content: 48.76

Gene sequence:

>1251_bases
ATGGATGATTTAACCAAATTGGGATTGACGGTAACTGAGTTACGCTTCGCCCTAGATACGTTTTACTTTTTGATTTCAGG
TGCACTCGTGATGTGGATGGCGGCGGGATTTGCCATGCTTGAGGCGGGCTTAGTGCGCTCGAAGAATACCACAGAGATCC
TAACTAAAAATGTCTGCTTGTATTCGATAGCTTGTGTCATGTACCTGATTGTTGGCTATAACATTATGTATGTTGATAAC
GTCGAGGGAGGATGGTTTCCCTCATTTGGGTCCTTGATTGGTTCTCAGTCAGAAGGGGCCGACCATGCACTAGAATCTGA
CTTCTTCTTTCAGGTGGTATTTGTTGCCACGGCAATGTCTATCGTATCGGGCGCGGTGGCTGAGCGCATGAAACTGTGGG
CCTTCTTAATTTTCTCAATCTTTATGACAGCGGTGATCTACCCGGTTGAAGGCTACTGGACTTGGGGCGGTGGTTTCTTG
TCGACGGCTGGCTTCGTCGATTTTGCTGGTAGTGGTATTGTGCATATGGCTGGCGCAGCTGCAGCGATTTCAGGTGTGTT
ATTACTAGGTGCTCGTAGAGGTAAATACGGCGCTAATGGTCAAATTAATCCCATTCCAGGTTCAAACTTACCCATGGCAA
CATTAGGCATGTTTATCTTGTGGATGGGCTGGTTTGGTTTTAATGGCGGTTCTCAATTGCTGCTATCTGACGCCGAAAAT
GCTAGCGCGGTGGCTAAGATTTTTGTTAATACCAACTCTGCGGCGGCTTTCGGTGCGATTTCGGCATTAATAGTATGTCG
ACTCATCTGGGGCAAGGCGGACTTAACCATGATCTTAAACGGCGCATTAGCCGGTCTGGTGGCGATCACCGCCGATCCTT
TATCGCCGTCACTAGCTATGGCTGGGGTGATTGGCTTGGCTGCTGGTGGTTTGGTGGTGTTCTCGATTGTGGCCTTCGAT
AGAATTAAGATTGATGATCCTGTTGGGGCTATCTCAGTCCATGGTGTGGCGGGTTTATTAGGCTTAATCTTGGTTCCAGT
ATCTAACAGCGAGGCTTCGTTTTTGACTCAGCTATATGGCGCGGCAGTGATTTTCGCTTGGGTGTTCTTCGCCTCGGCTG
CAGTATGGATGGCACTTAAGCTGACCATGGGGATCCGTGTTACCGAGGAAGAAGAGTATAACGGCATGGATGCATCCGAC
TGCGGTATCGACGCATACCCTGAGTTTGTCTCTGTTAAGAGTGGCAGCTAG

Upstream 100 bases:

>100_bases
GAAGATAGGTGACGGTAAGATTTTTGTGACAGATCTTGAGCAAGCTATCCGGATCAGAACTGGCGAACATGATAACGACG
CACTTTAAGGGGATAAGCGA

Downstream 100 bases:

>100_bases
TCATCTCGCAGTTAGATTAATAAGTCAAATAGACTTGGGTAAGGCTATCTTGTGAAGCTGTTTAGCCCAAACATGACTCA
AAACAATAGATTAAGACCCC

Product: Rh family protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDDLTKLGLTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCLYSIACVMYLIVGYNIMYVDN
VEGGWFPSFGSLIGSQSEGADHALESDFFFQVVFVATAMSIVSGAVAERMKLWAFLIFSIFMTAVIYPVEGYWTWGGGFL
STAGFVDFAGSGIVHMAGAAAAISGVLLLGARRGKYGANGQINPIPGSNLPMATLGMFILWMGWFGFNGGSQLLLSDAEN
ASAVAKIFVNTNSAAAFGAISALIVCRLIWGKADLTMILNGALAGLVAITADPLSPSLAMAGVIGLAAGGLVVFSIVAFD
RIKIDDPVGAISVHGVAGLLGLILVPVSNSEASFLTQLYGAAVIFAWVFFASAAVWMALKLTMGIRVTEEEEYNGMDASD
CGIDAYPEFVSVKSGS

Sequences:

>Translated_416_residues
MDDLTKLGLTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCLYSIACVMYLIVGYNIMYVDN
VEGGWFPSFGSLIGSQSEGADHALESDFFFQVVFVATAMSIVSGAVAERMKLWAFLIFSIFMTAVIYPVEGYWTWGGGFL
STAGFVDFAGSGIVHMAGAAAAISGVLLLGARRGKYGANGQINPIPGSNLPMATLGMFILWMGWFGFNGGSQLLLSDAEN
ASAVAKIFVNTNSAAAFGAISALIVCRLIWGKADLTMILNGALAGLVAITADPLSPSLAMAGVIGLAAGGLVVFSIVAFD
RIKIDDPVGAISVHGVAGLLGLILVPVSNSEASFLTQLYGAAVIFAWVFFASAAVWMALKLTMGIRVTEEEEYNGMDASD
CGIDAYPEFVSVKSGS
>Mature_416_residues
MDDLTKLGLTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCLYSIACVMYLIVGYNIMYVDN
VEGGWFPSFGSLIGSQSEGADHALESDFFFQVVFVATAMSIVSGAVAERMKLWAFLIFSIFMTAVIYPVEGYWTWGGGFL
STAGFVDFAGSGIVHMAGAAAAISGVLLLGARRGKYGANGQINPIPGSNLPMATLGMFILWMGWFGFNGGSQLLLSDAEN
ASAVAKIFVNTNSAAAFGAISALIVCRLIWGKADLTMILNGALAGLVAITADPLSPSLAMAGVIGLAAGGLVVFSIVAFD
RIKIDDPVGAISVHGVAGLLGLILVPVSNSEASFLTQLYGAAVIFAWVFFASAAVWMALKLTMGIRVTEEEEYNGMDASD
CGIDAYPEFVSVKSGS

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=418, Percent_Identity=30.1435406698565, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17550180, Length=452, Percent_Identity=33.8495575221239, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17550182, Length=434, Percent_Identity=32.7188940092166, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI133931100, Length=334, Percent_Identity=29.940119760479, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32564042, Length=426, Percent_Identity=27.9342723004695, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325396, Length=421, Percent_Identity=27.790973871734, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321559, Length=422, Percent_Identity=26.5402843601896, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324187, Length=409, Percent_Identity=25.9168704156479, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078314, Length=330, Percent_Identity=36.0606060606061, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43811; Mature: 43811

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLTKLGLTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCL
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YSIACVMYLIVGYNIMYVDNVEGGWFPSFGSLIGSQSEGADHALESDFFFQVVFVATAMS
HHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHH
IVSGAVAERMKLWAFLIFSIFMTAVIYPVEGYWTWGGGFLSTAGFVDFAGSGIVHMAGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCHHHCCCCCHHHHHHHH
AAISGVLLLGARRGKYGANGQINPIPGSNLPMATLGMFILWMGWFGFNGGSQLLLSDAEN
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
ASAVAKIFVNTNSAAAFGAISALIVCRLIWGKADLTMILNGALAGLVAITADPLSPSLAM
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCHHHH
AGVIGLAAGGLVVFSIVAFDRIKIDDPVGAISVHGVAGLLGLILVPVSNSEASFLTQLYG
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHEEECCCCHHHHHHHHHH
AAVIFAWVFFASAAVWMALKLTMGIRVTEEEEYNGMDASDCGIDAYPEFVSVKSGS
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCEEECCCC
>Mature Secondary Structure
MDDLTKLGLTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCL
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YSIACVMYLIVGYNIMYVDNVEGGWFPSFGSLIGSQSEGADHALESDFFFQVVFVATAMS
HHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHH
IVSGAVAERMKLWAFLIFSIFMTAVIYPVEGYWTWGGGFLSTAGFVDFAGSGIVHMAGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCHHHCCCCCHHHHHHHH
AAISGVLLLGARRGKYGANGQINPIPGSNLPMATLGMFILWMGWFGFNGGSQLLLSDAEN
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
ASAVAKIFVNTNSAAAFGAISALIVCRLIWGKADLTMILNGALAGLVAITADPLSPSLAM
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCHHHH
AGVIGLAAGGLVVFSIVAFDRIKIDDPVGAISVHGVAGLLGLILVPVSNSEASFLTQLYG
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHEEECCCCHHHHHHHHHH
AAVIFAWVFFASAAVWMALKLTMGIRVTEEEEYNGMDASDCGIDAYPEFVSVKSGS
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]