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The map label for this gene is cysJ [H]

Identifier: 157960481

GI number: 157960481

Start: 808052

End: 809848

Strand: Direct

Name: cysJ [H]

Synonym: Spea_0653

Alternate gene names: 157960481

Gene position: 808052-809848 (Clockwise)

Preceding gene: 157960476

Following gene: 157960482

Centisome position: 15.62

GC content: 51.14

Gene sequence:

>1797_bases
ATGTTGTTAAAAGAGCTTTCATCACTCGCTTCGCCGCTATCTAGTAGCCAAGTGGACAAGTTAAAGCAGCTCACTGCAGA
GCTGAATGCCGTGCAACTTTCTTGGGTTAGCGGCTATCTTGCTGCAACATCTGAGCAGGGAGTGGCAGGCTTGCCTCTGG
TTGAGGCTGGCTCGCAATCTGGAGCTGAACAAACCATCACTATTCTCTATGGTAGCCAGACGGGGAATGGTCGCGGTATT
GCCACCGAACTTGCCGAAAAGGCGCTGGCGCAAGGGTATGCGGTCAACCTCGTCTCTATGGGAGATTACAAGACACGACA
GCTTAAGCAGGAGAGTCTGCTCTTGTTAGTCGTAAGCACCCATGGCGAAGGTGAAGCGCCAGATGATGCTATCGAACTAC
ATAAGTTCTTAAGCTCTAAACGTGCCCCAAAACTGGATAACCTTAACTACTCAGTGCTGGCGTTGGGTGATTCAAGTTAT
GAGTTTTTCTGTCAAACAGGTAAAGACTTCGAGACTCGTCTTAACGCATTAGGGGCGACTTCGATTGCGCCTCTGGTCGA
GTGCGATGTCGACTATGAAGCTCAAGCCGAGCAGTGGCAAGCGGATGTGTTAGAGGCGGTGAAGCCATTAGTGCAGAGCT
CAGGAGCCAGTGTGGTCTCTATCGCTAGCTCTCAGACATCAGGCTCTAGTTACACCAAACAAAAGCCCTACACGGCAGAA
TTGCTGGTGAGTCAAAAACTAACTGGCCGCGACTCTGATAGAGACGTGCGCCATGTTGAAATTGAGCTGGGTGAGTCAGG
AATAAACTACCAAGCCGGTGATGCACTCGGCGTATGGTTTACTAACTCACAAGAACTGGTTGCTGAGCTACTCGAAGCCT
TAGCGCTCGATGGCGATGAGTCTGTCTCTGTTGGTAGCGATAGCTTGAGTCTGCGTGAAGCGCTAACCAGTAAGAAAGAG
CTGACCCAGCTTTATCCAGGTTTAGTCACTGGTTGGGCGGAACTGAGTCAGAATGCTGAGCTGTTGGCCATTAGCGCCGA
TAAAGCGTTAACCCGAGAGTTTGTCAATAACAATCAAGTGGCCGATCTAGTTCGTCAGTACCCGGCTAAGGTGGCTGCAG
AGCAGTTTATTGAGCTACTGCGCGGCATTACCCCAAGACTCTACTCAATTGCATCGAGTCAGGCTGAGGTCGAGTCCGAG
GTGCACTTGACCGTGGCGCTGGTCGAAGATGAACGTGATGGTGTAACCCGCTTTGGTGGTGCATCTCAGTTCTTAGCGGC
CGCCGAAGAGGGTCAAGAAGTACAAGTTTATGTCGAGCCTAATAATCACTTCCGTCTACCAGAAAACCCTGAAACGCCGG
TGATCATGGTGGGCCCTGGCACAGGTGTGGCTCCTTTCAGAGCCTTCATGCAAGAACGTGCTGCACTGGGCGCCGAGGGT
AATACTTGGTTGATCTTCGGTAACCCGCATTTTGAGCAAGACTTCCTCTACCAAACAGAGTGGCAGCAGTATCTGCAAAG
CGGCGAATTATCGCGTATCGACTTGGCGTTTTCACGGGATCAAGAGCACAAGATCTATGTGCAGCACAAGATCGCCGAGC
AAGGTGAGGCGCTTTGGCAATGGTTAGCGTCCGGTGCGCACTTCTATGTCTGTGGTGACGCCGAACGTATGGCGAAAGAT
GTCCACCAAGCGCTGCTCGATGTGGCCGTTAAATACGGTAATAAAACTCAAGAGCAAGCGGCTGAATATCTCGAAGAGCT
AAGAGCGGCCAAGCGCTATCAAAAAGACGTTTACTAA

Upstream 100 bases:

>100_bases
GTATATTCCAAAATCGAATTAAAAATTATCTTTTATTCTTTTTTTATTATTTAAGGAGGGGCTAAGCTGGCCAACATTAA
ACTGATACAGGGCCGTTTCT

Downstream 100 bases:

>100_bases
GACTCAGCTTTAAACGAGTAAAGCAATGATGAGCGACGCTAGAGTTAACTAGCGCGAGTGCAGAAAAGAATTTTAAGAGA
GTACCTAGCTATGAGTGAGC

Product: sulfite reductase (NADPH) flavoprotein subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MLLKELSSLASPLSSSQVDKLKQLTAELNAVQLSWVSGYLAATSEQGVAGLPLVEAGSQSGAEQTITILYGSQTGNGRGI
ATELAEKALAQGYAVNLVSMGDYKTRQLKQESLLLLVVSTHGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALGDSSY
EFFCQTGKDFETRLNALGATSIAPLVECDVDYEAQAEQWQADVLEAVKPLVQSSGASVVSIASSQTSGSSYTKQKPYTAE
LLVSQKLTGRDSDRDVRHVEIELGESGINYQAGDALGVWFTNSQELVAELLEALALDGDESVSVGSDSLSLREALTSKKE
LTQLYPGLVTGWAELSQNAELLAISADKALTREFVNNNQVADLVRQYPAKVAAEQFIELLRGITPRLYSIASSQAEVESE
VHLTVALVEDERDGVTRFGGASQFLAAAEEGQEVQVYVEPNNHFRLPENPETPVIMVGPGTGVAPFRAFMQERAALGAEG
NTWLIFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQEHKIYVQHKIAEQGEALWQWLASGAHFYVCGDAERMAKD
VHQALLDVAVKYGNKTQEQAAEYLEELRAAKRYQKDVY

Sequences:

>Translated_598_residues
MLLKELSSLASPLSSSQVDKLKQLTAELNAVQLSWVSGYLAATSEQGVAGLPLVEAGSQSGAEQTITILYGSQTGNGRGI
ATELAEKALAQGYAVNLVSMGDYKTRQLKQESLLLLVVSTHGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALGDSSY
EFFCQTGKDFETRLNALGATSIAPLVECDVDYEAQAEQWQADVLEAVKPLVQSSGASVVSIASSQTSGSSYTKQKPYTAE
LLVSQKLTGRDSDRDVRHVEIELGESGINYQAGDALGVWFTNSQELVAELLEALALDGDESVSVGSDSLSLREALTSKKE
LTQLYPGLVTGWAELSQNAELLAISADKALTREFVNNNQVADLVRQYPAKVAAEQFIELLRGITPRLYSIASSQAEVESE
VHLTVALVEDERDGVTRFGGASQFLAAAEEGQEVQVYVEPNNHFRLPENPETPVIMVGPGTGVAPFRAFMQERAALGAEG
NTWLIFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQEHKIYVQHKIAEQGEALWQWLASGAHFYVCGDAERMAKD
VHQALLDVAVKYGNKTQEQAAEYLEELRAAKRYQKDVY
>Mature_598_residues
MLLKELSSLASPLSSSQVDKLKQLTAELNAVQLSWVSGYLAATSEQGVAGLPLVEAGSQSGAEQTITILYGSQTGNGRGI
ATELAEKALAQGYAVNLVSMGDYKTRQLKQESLLLLVVSTHGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALGDSSY
EFFCQTGKDFETRLNALGATSIAPLVECDVDYEAQAEQWQADVLEAVKPLVQSSGASVVSIASSQTSGSSYTKQKPYTAE
LLVSQKLTGRDSDRDVRHVEIELGESGINYQAGDALGVWFTNSQELVAELLEALALDGDESVSVGSDSLSLREALTSKKE
LTQLYPGLVTGWAELSQNAELLAISADKALTREFVNNNQVADLVRQYPAKVAAEQFIELLRGITPRLYSIASSQAEVESE
VHLTVALVEDERDGVTRFGGASQFLAAAEEGQEVQVYVEPNNHFRLPENPETPVIMVGPGTGVAPFRAFMQERAALGAEG
NTWLIFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQEHKIYVQHKIAEQGEALWQWLASGAHFYVCGDAERMAKD
VHQALLDVAVKYGNKTQEQAAEYLEELRAAKRYQKDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=599, Percent_Identity=30.8848080133556, Blast_Score=250, Evalue=3e-66,
Organism=Homo sapiens, GI221316709, Length=598, Percent_Identity=30.6020066889632, Blast_Score=245, Evalue=8e-65,
Organism=Homo sapiens, GI221316705, Length=608, Percent_Identity=30.4276315789474, Blast_Score=242, Evalue=7e-64,
Organism=Homo sapiens, GI127139033, Length=617, Percent_Identity=30.4700162074554, Blast_Score=236, Evalue=5e-62,
Organism=Homo sapiens, GI221316707, Length=526, Percent_Identity=31.5589353612167, Blast_Score=220, Evalue=4e-57,
Organism=Homo sapiens, GI24041029, Length=594, Percent_Identity=29.6296296296296, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI10835173, Length=643, Percent_Identity=27.0606531881804, Blast_Score=174, Evalue=3e-43,
Organism=Homo sapiens, GI40254422, Length=643, Percent_Identity=26.7496111975117, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI169790956, Length=419, Percent_Identity=29.8329355608592, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI169790958, Length=419, Percent_Identity=29.8329355608592, Blast_Score=153, Evalue=4e-37,
Organism=Escherichia coli, GI1789123, Length=590, Percent_Identity=52.0338983050847, Blast_Score=605, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI17554134, Length=605, Percent_Identity=28.4297520661157, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17566446, Length=574, Percent_Identity=28.397212543554, Blast_Score=168, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17531441, Length=256, Percent_Identity=26.171875, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321832, Length=638, Percent_Identity=28.9968652037618, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321143, Length=391, Percent_Identity=31.7135549872123, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325305, Length=633, Percent_Identity=22.9067930489731, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322597, Length=164, Percent_Identity=29.8780487804878, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17137192, Length=606, Percent_Identity=29.2079207920792, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24582192, Length=517, Percent_Identity=28.6266924564797, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24660907, Length=594, Percent_Identity=26.9360269360269, Blast_Score=188, Evalue=9e-48,
Organism=Drosophila melanogaster, GI24660903, Length=594, Percent_Identity=26.9360269360269, Blast_Score=188, Evalue=9e-48,
Organism=Drosophila melanogaster, GI78706872, Length=521, Percent_Identity=27.831094049904, Blast_Score=153, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24583543, Length=521, Percent_Identity=27.831094049904, Blast_Score=153, Evalue=4e-37,
Organism=Drosophila melanogaster, GI78706876, Length=521, Percent_Identity=27.831094049904, Blast_Score=153, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24647438, Length=409, Percent_Identity=26.6503667481663, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 65364; Mature: 65364

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKELSSLASPLSSSQVDKLKQLTAELNAVQLSWVSGYLAATSEQGVAGLPLVEAGSQS
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEHHHHHHCCCCCCCCCCCEEECCCCC
GAEQTITILYGSQTGNGRGIATELAEKALAQGYAVNLVSMGDYKTRQLKQESLLLLVVST
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCEEEEEEEC
HGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALGDSSYEFFCQTGKDFETRLNALGAT
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHCCCC
SIAPLVECDVDYEAQAEQWQADVLEAVKPLVQSSGASVVSIASSQTSGSSYTKQKPYTAE
CCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHH
LLVSQKLTGRDSDRDVRHVEIELGESGINYQAGDALGVWFTNSQELVAELLEALALDGDE
HHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCC
SVSVGSDSLSLREALTSKKELTQLYPGLVTGWAELSQNAELLAISADKALTREFVNNNQV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCHH
ADLVRQYPAKVAAEQFIELLRGITPRLYSIASSQAEVESEVHLTVALVEDERDGVTRFGG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCEEEEEEEEECCCCCCHHCCC
ASQFLAAAEEGQEVQVYVEPNNHFRLPENPETPVIMVGPGTGVAPFRAFMQERAALGAEG
HHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC
NTWLIFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQEHKIYVQHKIAEQGEALWQ
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHH
WLASGAHFYVCGDAERMAKDVHQALLDVAVKYGNKTQEQAAEYLEELRAAKRYQKDVY
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLKELSSLASPLSSSQVDKLKQLTAELNAVQLSWVSGYLAATSEQGVAGLPLVEAGSQS
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEHHHHHHCCCCCCCCCCCEEECCCCC
GAEQTITILYGSQTGNGRGIATELAEKALAQGYAVNLVSMGDYKTRQLKQESLLLLVVST
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCEEEEEEEC
HGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALGDSSYEFFCQTGKDFETRLNALGAT
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHCCCC
SIAPLVECDVDYEAQAEQWQADVLEAVKPLVQSSGASVVSIASSQTSGSSYTKQKPYTAE
CCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHH
LLVSQKLTGRDSDRDVRHVEIELGESGINYQAGDALGVWFTNSQELVAELLEALALDGDE
HHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCC
SVSVGSDSLSLREALTSKKELTQLYPGLVTGWAELSQNAELLAISADKALTREFVNNNQV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCHH
ADLVRQYPAKVAAEQFIELLRGITPRLYSIASSQAEVESEVHLTVALVEDERDGVTRFGG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCEEEEEEEEECCCCCCHHCCC
ASQFLAAAEEGQEVQVYVEPNNHFRLPENPETPVIMVGPGTGVAPFRAFMQERAALGAEG
HHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC
NTWLIFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQEHKIYVQHKIAEQGEALWQ
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHH
WLASGAHFYVCGDAERMAKDVHQALLDVAVKYGNKTQEQAAEYLEELRAAKRYQKDVY
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]