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The map label for this gene is hldE [H]

Identifier: 157960474

GI number: 157960474

Start: 799745

End: 801193

Strand: Direct

Name: hldE [H]

Synonym: Spea_0646

Alternate gene names: 157960474

Gene position: 799745-801193 (Clockwise)

Preceding gene: 157960472

Following gene: 157960475

Centisome position: 15.46

GC content: 51.48

Gene sequence:

>1449_bases
GTGATAGATATAGGCGTTATGAAGATTTCTCTGCCAGCATTTGAAAAAGCCAAGGTTCTCGTTGTCGGTGACGTGATGTT
AGATCGCTATTGGGCAGGGCCTACAGCGCGGATCTCACCTGAAGCGCCTGTACCCGTTGTTAAAGTTGAGCAACTTGAAG
ACAGACCCGGCGGCGCAGCGAACGTGGCGATCAATATCGCGACCTTAGGTGGCAGCGCAAGCCTTGCTGGCATCGTTGGT
GTAGATGAAACCGCCGATGCATTAACTGTGGGGGTGCGTGCCTTAGGTGTTGAGCCTAACTGGCACAAGGTGGCCGATAA
GCCAACCATCACTAAGTTACGCGTCATGTCTCGCAATCAACAGTTATTAAGACTCGACTTTGAAGAGAGCTACTCTCAAG
CAGAGAGCGATGCGCTGTTAGCACAAAGTTTATCTGAGCTAGATAAGGTCGATGTGGCGGTGTTGTCTGACTATGCCAAG
GGCGCCTTGATTTCACCACAAAGCTTTATTGAAGCGGCTAACGCTAAAGGCGTTAAAGTGCTGGTGGATCCTAAGGGGAG
CGATTTTTCAAAGTATCGCGGCGCGTACCTGCTAACGCCAAATATGAGTGAGTTTGAGGTCGTGGTCGGTAAAGTGGAAT
CCGAAGCCGATTTAGTGGCTAAGGCGCAAGGTCTGTTACAAGCCTTCGATCTAACCGCCATGCTAGTGACTCGCTCTGAA
AAGGGCATGACGCTAATCACTAAAGATCAACCTGAGCTGCACATTCCTACCGTTGCTCGCGAAGTCTACGATGTTACTGG
CGCCGGTGACACGGTTATTTCTGCGCTGGCTACGGCAATCGCTGCTGGTAGCGACTTGCCACAGGCTTGTGCGATTGCCA
ATACGGCTGCGGGCATTGTGGTGGCTAAGCTTGGCACATCGACAGTGACACGTCTTGAGCTGATTGAAGCGCTATCTCTA
AGCCATGGTGAGTCAGGTTTTGGCGCGGTCAGTGAAGATCAATTGGCTTATGCCCTTGAGCAGGCAAAGCTACGGGGTGA
ACGTGTGGTGATGACCAATGGCTGCTTTGATATCTTGCATGCTGGGCATGTGAGTTACCTGCAGCAGGCTCGCGCTTTAG
GCGATCGGTTAATTGTGGCGGTTAATACAGACGCTTCGGTTAAGCGCCTGAAAGGTGAAGGTCGCCCGGTTAACAGCGAA
GATAGACGTATGACGGTATTGGCAGCGCTTGCATCTGTCGATTGGGTGGTACCGTTTAGCGAGGATACGCCGCAGCGCAT
CATCTCGCGCCTACTGCCAGATTTGTTGGTGAAAGGTGGCGATTATAAGGTGGAAGATATCGCTGGTGGCAGTGAGGTAA
TGGCAGCTGGTGGTCAGGTTAAAGTCTTAGGCTTTGAAGATGGCATCTCAACCACGGCCATTATCCAAAACATCATGGCT
AACCAGTAG

Upstream 100 bases:

>100_bases
TTGAGCTTTTTCTACCACGTAATTCTCGGATCCATTGAAAAATTACTCATTATTCTAACTCAAAAGTGATTAAAATTAGG
TACAATCAGGATTAAATTTT

Downstream 100 bases:

>100_bases
CCATTGATGATTAGTCGCTAGTGATTGACTATAAAGCACAATGATAAGCTCGCTCGCAGCGACTGCCGCTACTGCTATTT
TGGCAACACATATGTCAGCG

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MIDIGVMKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAANVAINIATLGGSASLAGIVG
VDETADALTVGVRALGVEPNWHKVADKPTITKLRVMSRNQQLLRLDFEESYSQAESDALLAQSLSELDKVDVAVLSDYAK
GALISPQSFIEAANAKGVKVLVDPKGSDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLQAFDLTAMLVTRSE
KGMTLITKDQPELHIPTVAREVYDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIVVAKLGTSTVTRLELIEALSL
SHGESGFGAVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNSE
DRRMTVLAALASVDWVVPFSEDTPQRIISRLLPDLLVKGGDYKVEDIAGGSEVMAAGGQVKVLGFEDGISTTAIIQNIMA
NQ

Sequences:

>Translated_482_residues
MIDIGVMKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAANVAINIATLGGSASLAGIVG
VDETADALTVGVRALGVEPNWHKVADKPTITKLRVMSRNQQLLRLDFEESYSQAESDALLAQSLSELDKVDVAVLSDYAK
GALISPQSFIEAANAKGVKVLVDPKGSDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLQAFDLTAMLVTRSE
KGMTLITKDQPELHIPTVAREVYDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIVVAKLGTSTVTRLELIEALSL
SHGESGFGAVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNSE
DRRMTVLAALASVDWVVPFSEDTPQRIISRLLPDLLVKGGDYKVEDIAGGSEVMAAGGQVKVLGFEDGISTTAIIQNIMA
NQ
>Mature_482_residues
MIDIGVMKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAANVAINIATLGGSASLAGIVG
VDETADALTVGVRALGVEPNWHKVADKPTITKLRVMSRNQQLLRLDFEESYSQAESDALLAQSLSELDKVDVAVLSDYAK
GALISPQSFIEAANAKGVKVLVDPKGSDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLQAFDLTAMLVTRSE
KGMTLITKDQPELHIPTVAREVYDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIVVAKLGTSTVTRLELIEALSL
SHGESGFGAVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNSE
DRRMTVLAALASVDWVVPFSEDTPQRIISRLLPDLLVKGGDYKVEDIAGGSEVMAAGGQVKVLGFEDGISTTAIIQNIMA
NQ

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI296841136, Length=132, Percent_Identity=32.5757575757576, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4505651, Length=132, Percent_Identity=32.5757575757576, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789432, Length=476, Percent_Identity=59.8739495798319, Blast_Score=573, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 50947; Mature: 50947

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDIGVMKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAA
CCEEEEEEEECCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEEEHHCCCCCCCE
NVAINIATLGGSASLAGIVGVDETADALTVGVRALGVEPNWHKVADKPTITKLRVMSRNQ
EEEEEEEEECCCCCEEEEECCCCCHHHHHHHHEEECCCCCCHHCCCCCCHHEEEECCCCC
QLLRLDFEESYSQAESDALLAQSLSELDKVDVAVLSDYAKGALISPQSFIEAANAKGVKV
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHCCCCCEEE
LVDPKGSDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLQAFDLTAMLVTRSE
EECCCCCCHHHHCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECC
KGMTLITKDQPELHIPTVAREVYDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIV
CCCEEEECCCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEE
VAKLGTSTVTRLELIEALSLSHGESGFGAVSEDQLAYALEQAKLRGERVVMTNGCFDILH
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHH
AGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNSEDRRMTVLAALASVDWVVPFS
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECC
EDTPQRIISRLLPDLLVKGGDYKVEDIAGGSEVMAAGGQVKVLGFEDGISTTAIIQNIMA
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHEECCCEEEEEEECCCCHHHHHHHHHHC
NQ
CC
>Mature Secondary Structure
MIDIGVMKISLPAFEKAKVLVVGDVMLDRYWAGPTARISPEAPVPVVKVEQLEDRPGGAA
CCEEEEEEEECCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEEEHHCCCCCCCE
NVAINIATLGGSASLAGIVGVDETADALTVGVRALGVEPNWHKVADKPTITKLRVMSRNQ
EEEEEEEEECCCCCEEEEECCCCCHHHHHHHHEEECCCCCCHHCCCCCCHHEEEECCCCC
QLLRLDFEESYSQAESDALLAQSLSELDKVDVAVLSDYAKGALISPQSFIEAANAKGVKV
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHCCCCCEEE
LVDPKGSDFSKYRGAYLLTPNMSEFEVVVGKVESEADLVAKAQGLLQAFDLTAMLVTRSE
EECCCCCCHHHHCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECC
KGMTLITKDQPELHIPTVAREVYDVTGAGDTVISALATAIAAGSDLPQACAIANTAAGIV
CCCEEEECCCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEE
VAKLGTSTVTRLELIEALSLSHGESGFGAVSEDQLAYALEQAKLRGERVVMTNGCFDILH
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHH
AGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNSEDRRMTVLAALASVDWVVPFS
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECC
EDTPQRIISRLLPDLLVKGGDYKVEDIAGGSEVMAAGGQVKVLGFEDGISTTAIIQNIMA
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHEECCCEEEEEEECCCCHHHHHHHHHHC
NQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA