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The map label for this gene is rpfG [H]

Identifier: 157960458

GI number: 157960458

Start: 779176

End: 780195

Strand: Direct

Name: rpfG [H]

Synonym: Spea_0629

Alternate gene names: 157960458

Gene position: 779176-780195 (Clockwise)

Preceding gene: 157960457

Following gene: 157960461

Centisome position: 15.06

GC content: 48.92

Gene sequence:

>1020_bases
ATGGATAAGGCCACCGTTTTAGTTGTGGATGATACCCCTGAGAATATTGATATTTTGGTTGGGATCTTAGGCGGCGATTA
TAAGGTTAAGGTGGCAATTGATGGTCCTAAAGCCTTGGCGCTAGCCAGTAAAAGTTCACCGGATCTTATATTGCTGGATG
TGATGATGCCGGGGATGAATGGCTATGAGGTGTGTAAGCGACTCAAACAGGAGCCGTTAACCAACCATATTCCGGTTATT
TTTGTTACCGCCCTTACTGAGGTGGCCGATGAGACCCAGGGGTTTGAGCTTGGCGCCGTCGATTACATTACCAAACCCGT
TAGTGCGCCAGTGGTTAAGGCTCGGGTTAAGACCCATTTAGCCCTATATGATCAGAAACGCCTACTCGAGCAGCAAGTTA
AGGCACGCACCAAGGAGCTAGAGGAAACTCGCTTCGAGATCATTCGTCGTCTTGGTCGCGCCGCCGAATATAAAGATAAT
GAAACGGGCTTACATGTGGTGAGAATGAGCCACTATGCGCGTTTGTTAGCTATGCAGTCGGGTCTGCCTGAGCATTACTG
CGAGCTTATCTATAACGCCGCGCCTATGCATGATATCGGCAAAATTGGCACGCCAGACTCTATCTTGAAGAAGCCTGCTA
GTTTGGATGATAACGAATGGGACGAGATGCAGCGCCATGCTGAAATCGGCGCCGAGATCATAGGTGAGCATAATGATCCG
CTATTAGAAATGGCTCGCCGAATCGCACTGACTCATCATGAGAAGTGGGATGGCAGTGGTTACCCTTATGGTTTATCCGG
TGAAGATATCCCTATTGATGGCCGCATTGTGGCGATTGCCGACGTGTTCGATGCCTTAACCTCGATTCGTCCCTATAAAA
AGGCGTGGACGATTGAGGCGACCATGGCTTTAATAGAGGATGAGTCGGGTAAGCATTTCGATCCAGAGTTGGTGAAGCAT
TTTAAGCTGATTATCGATGAGGTGACCAAGGTTCGCGATGCCCATAATGAGTCGGGGTAG

Upstream 100 bases:

>100_bases
CAGCGATGAGCTAAGGCCGTTAGATTAGAAAAAGGCTCGCATCGCTTAGCTATACTTGATGTTGTTATCGAGAGTGAGAA
CTGAAAGGAGAATCGGTTTA

Downstream 100 bases:

>100_bases
TCAGAGTCAGTGACTCTGGATAACTCTGAATTATTTGAGCTGAAACTAGCTGAAAACAAAAAGGCGCGGTAAGCGCCTTT
TCTGTTTTAAGCCAAAGCAG

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MDKATVLVVDDTPENIDILVGILGGDYKVKVAIDGPKALALASKSSPDLILLDVMMPGMNGYEVCKRLKQEPLTNHIPVI
FVTALTEVADETQGFELGAVDYITKPVSAPVVKARVKTHLALYDQKRLLEQQVKARTKELEETRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLAMQSGLPEHYCELIYNAAPMHDIGKIGTPDSILKKPASLDDNEWDEMQRHAEIGAEIIGEHNDP
LLEMARRIALTHHEKWDGSGYPYGLSGEDIPIDGRIVAIADVFDALTSIRPYKKAWTIEATMALIEDESGKHFDPELVKH
FKLIIDEVTKVRDAHNESG

Sequences:

>Translated_339_residues
MDKATVLVVDDTPENIDILVGILGGDYKVKVAIDGPKALALASKSSPDLILLDVMMPGMNGYEVCKRLKQEPLTNHIPVI
FVTALTEVADETQGFELGAVDYITKPVSAPVVKARVKTHLALYDQKRLLEQQVKARTKELEETRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLAMQSGLPEHYCELIYNAAPMHDIGKIGTPDSILKKPASLDDNEWDEMQRHAEIGAEIIGEHNDP
LLEMARRIALTHHEKWDGSGYPYGLSGEDIPIDGRIVAIADVFDALTSIRPYKKAWTIEATMALIEDESGKHFDPELVKH
FKLIIDEVTKVRDAHNESG
>Mature_339_residues
MDKATVLVVDDTPENIDILVGILGGDYKVKVAIDGPKALALASKSSPDLILLDVMMPGMNGYEVCKRLKQEPLTNHIPVI
FVTALTEVADETQGFELGAVDYITKPVSAPVVKARVKTHLALYDQKRLLEQQVKARTKELEETRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARLLAMQSGLPEHYCELIYNAAPMHDIGKIGTPDSILKKPASLDDNEWDEMQRHAEIGAEIIGEHNDP
LLEMARRIALTHHEKWDGSGYPYGLSGEDIPIDGRIVAIADVFDALTSIRPYKKAWTIEATMALIEDESGKHFDPELVKH
FKLIIDEVTKVRDAHNESG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=142, Percent_Identity=36.6197183098592, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=39.6694214876033, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=37.6068376068376, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI145693157, Length=126, Percent_Identity=38.8888888888889, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=34.4827586206897, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=32.7586206896552, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=34.7107438016529, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=36.1904761904762, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 37851; Mature: 37851

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKATVLVVDDTPENIDILVGILGGDYKVKVAIDGPKALALASKSSPDLILLDVMMPGMN
CCCEEEEEECCCCCCEEEEEEEECCCEEEEEEECCCCEEEEECCCCCCEEEEEEECCCCC
GYEVCKRLKQEPLTNHIPVIFVTALTEVADETQGFELGAVDYITKPVSAPVVKARVKTHL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHH
ALYDQKRLLEQQVKARTKELEETRFEIIRRLGRAAEYKDNETGLHVVRMSHYARLLAMQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHC
GLPEHYCELIYNAAPMHDIGKIGTPDSILKKPASLDDNEWDEMQRHAEIGAEIIGEHNDP
CCHHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCH
LLEMARRIALTHHEKWDGSGYPYGLSGEDIPIDGRIVAIADVFDALTSIRPYKKAWTIEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHEEHHH
TMALIEDESGKHFDPELVKHFKLIIDEVTKVRDAHNESG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDKATVLVVDDTPENIDILVGILGGDYKVKVAIDGPKALALASKSSPDLILLDVMMPGMN
CCCEEEEEECCCCCCEEEEEEEECCCEEEEEEECCCCEEEEECCCCCCEEEEEEECCCCC
GYEVCKRLKQEPLTNHIPVIFVTALTEVADETQGFELGAVDYITKPVSAPVVKARVKTHL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHH
ALYDQKRLLEQQVKARTKELEETRFEIIRRLGRAAEYKDNETGLHVVRMSHYARLLAMQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHC
GLPEHYCELIYNAAPMHDIGKIGTPDSILKKPASLDDNEWDEMQRHAEIGAEIIGEHNDP
CCHHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCH
LLEMARRIALTHHEKWDGSGYPYGLSGEDIPIDGRIVAIADVFDALTSIRPYKKAWTIEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHEEHHH
TMALIEDESGKHFDPELVKHFKLIIDEVTKVRDAHNESG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]