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The map label for this gene is metB [H]

Identifier: 157960389

GI number: 157960389

Start: 686767

End: 687927

Strand: Direct

Name: metB [H]

Synonym: Spea_0560

Alternate gene names: 157960389

Gene position: 686767-687927 (Clockwise)

Preceding gene: 157960386

Following gene: 157960390

Centisome position: 13.27

GC content: 49.27

Gene sequence:

>1161_bases
ATGACTGAGCGTAAATTAGCCACTGCGGCTGTACGTCAAGGAATTGAGTCCGATAGCCAGCATGGTGCAGTAGTACCGCC
GATCTATCTTTCTACTAATTACTCTTTTGATGGTCATAAAAACCCAAGAGACTTCGACTATAGCCGCTCAGGTAACCCTA
CACGTTCTATTTTAGGTGATGCCATTGCCGAGCTTGAAAAAGGCTCTACGGGCGTAGTGACTTGCACCGGTATGGCGGCA
ATAACGTTGGCCACTAGCTTGCTTGGACCTGATGATCTTCTTGTTGTTCCCCATGATTGTTATGGTGGTAGTTACCGCCT
ATTTACTAATTTGGCCAAAAAAGGTCAGTTCAAGTTATTAGTGGTCGATCAAACCGACTCACAGGCCCTTAATCAGGCTA
TAGCGCAAAAACCTAAGATGGTATGGCTAGAGACGCCGTCGAATCCACTGCTGCGTGTGGTCGACATTAAGGCCATCTGT
GCTGCGAGCCATGAAGTGGGCGCACTGGTGGTTGTCGATAACACTTTCCTGTCGCCGATTTTACAGCAGCCGTTATTACT
TGGCGCCGATATAGTGATCCATTCCACCACCAAATATATCAATGGTCATAGCGATGTGGTTGGCGGCGCGGTCGTGGCAA
AGGATCCTGAGTTAGGTGAACTGCTTCATTGGTGGTCAAACACCCTAGGGCTAACGGGGTCAGCGTTTGACAGCTACCTG
ACTTTGCGCGGAATTCGTACACTTGCAGTACGAATTCGCGAACATCAGGCGAATGCGCAGCGAATTCTTGATACCCTTGT
TAACCATCAGGCCGTCTCCAAGATATACTACCCGGGGCTGAAAGAGCATCCGGGGCATGAGATTGCGGCTAAACAGCAAA
AGGGCTTCGGTGCCATGCTGAGCTTTGAACTTAAGGGTGGAGAACAAGAGTTAGTCACATTCTTAAAAAATCTTAGCTGT
TTTTCAGTTGCAGAGAGCCTGGGTGGCGTAGAGAGTTTAGTCGCCGTTCCTGCAACCATGACGCACAGAGCGATGGATCC
AGAGGCTCGCGCCGAAGCTGGCGTTAAAGATACGCTTATTCGTCTGTCTGTGGGTATCGAAGATGCCGATGATCTTGTTG
CCGATATTGAAGCTGCGCTTAATGCCGCAGCCGCTTGTTAA

Upstream 100 bases:

>100_bases
CGTCTAAACGGCTAAAGTGGTTGACGCAGGCTTAGTGCTTAGGTACATTTAGCCGTCCAGACATCTCTTGAGTGAAAATT
AACAGGAAAAGGAGTAGTAC

Downstream 100 bases:

>100_bases
TTAAAGGGGTATACGATGGCACGCTGTCACTTACATAAATTTGGTGGCTCAAGTCTTGCTGATGCAGACTGTTACCGTCG
CGTGGCTCATATTCTGCTGA

Product: O-succinylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTERKLATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHKNPRDFDYSRSGNPTRSILGDAIAELEKGSTGVVTCTGMAA
ITLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLLVVDQTDSQALNQAIAQKPKMVWLETPSNPLLRVVDIKAIC
AASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYL
TLRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIAAKQQKGFGAMLSFELKGGEQELVTFLKNLSC
FSVAESLGGVESLVAVPATMTHRAMDPEARAEAGVKDTLIRLSVGIEDADDLVADIEAALNAAAAC

Sequences:

>Translated_386_residues
MTERKLATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHKNPRDFDYSRSGNPTRSILGDAIAELEKGSTGVVTCTGMAA
ITLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLLVVDQTDSQALNQAIAQKPKMVWLETPSNPLLRVVDIKAIC
AASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYL
TLRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIAAKQQKGFGAMLSFELKGGEQELVTFLKNLSC
FSVAESLGGVESLVAVPATMTHRAMDPEARAEAGVKDTLIRLSVGIEDADDLVADIEAALNAAAAC
>Mature_385_residues
TERKLATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHKNPRDFDYSRSGNPTRSILGDAIAELEKGSTGVVTCTGMAAI
TLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLLVVDQTDSQALNQAIAQKPKMVWLETPSNPLLRVVDIKAICA
ASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYLT
LRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIAAKQQKGFGAMLSFELKGGEQELVTFLKNLSCF
SVAESLGGVESLVAVPATMTHRAMDPEARAEAGVKDTLIRLSVGIEDADDLVADIEAALNAAAAC

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=382, Percent_Identity=42.9319371727749, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI299473759, Length=382, Percent_Identity=40.8376963350785, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI25453487, Length=381, Percent_Identity=37.5328083989501, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=61.6797900262467, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789383, Length=396, Percent_Identity=31.0606060606061, Blast_Score=164, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17538055, Length=366, Percent_Identity=44.5355191256831, Blast_Score=301, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI17560056, Length=366, Percent_Identity=43.9890710382514, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI71980708, Length=375, Percent_Identity=32.8, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=42.1188630490956, Blast_Score=284, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=35.7142857142857, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323333, Length=422, Percent_Identity=31.5165876777251, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=32.1608040201005, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=27.0769230769231, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322970, Length=274, Percent_Identity=27.3722627737226, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322590, Length=277, Percent_Identity=26.7148014440433, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323557, Length=242, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922554, Length=382, Percent_Identity=40.8376963350785, Blast_Score=301, Evalue=5e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41348; Mature: 41216

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERKLATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHKNPRDFDYSRSGNPTRSILGD
CCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
AIAELEKGSTGVVTCTGMAAITLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLL
HHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
VVDQTDSQALNQAIAQKPKMVWLETPSNPLLRVVDIKAICAASHEVGALVVVDNTFLSPI
EEECCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHHCCCCCCEEEEECCHHHHHH
LQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYL
HHCHHHHCCEEEEECCHHHCCCCCCHHCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHH
TLRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIAAKQQKGFGAML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEE
SFELKGGEQELVTFLKNLSCFSVAESLGGVESLVAVPATMTHRAMDPEARAEAGVKDTLI
EEEECCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCHHHHHCCCCHHHHHCCCHHEEE
RLSVGIEDADDLVADIEAALNAAAAC
EEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TERKLATAAVRQGIESDSQHGAVVPPIYLSTNYSFDGHKNPRDFDYSRSGNPTRSILGD
CCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
AIAELEKGSTGVVTCTGMAAITLATSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLL
HHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
VVDQTDSQALNQAIAQKPKMVWLETPSNPLLRVVDIKAICAASHEVGALVVVDNTFLSPI
EEECCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHHCCCCCCEEEEECCHHHHHH
LQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDPELGELLHWWSNTLGLTGSAFDSYL
HHCHHHHCCEEEEECCHHHCCCCCCHHCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHH
TLRGIRTLAVRIREHQANAQRILDTLVNHQAVSKIYYPGLKEHPGHEIAAKQQKGFGAML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEE
SFELKGGEQELVTFLKNLSCFSVAESLGGVESLVAVPATMTHRAMDPEARAEAGVKDTLI
EEEECCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCHHHHHCCCCHHHHHCCCHHEEE
RLSVGIEDADDLVADIEAALNAAAAC
EEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]