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The map label for this gene is nifS1 [H]

Identifier: 157960373

GI number: 157960373

Start: 668459

End: 670744

Strand: Reverse

Name: nifS1 [H]

Synonym: Spea_0544

Alternate gene names: 157960373

Gene position: 670744-668459 (Counterclockwise)

Preceding gene: 157960374

Following gene: 157960371

Centisome position: 12.96

GC content: 48.78

Gene sequence:

>2286_bases
ATGCATAACCACAATGAACTGACCCAAGTTTATCTCGACGCAAATGCAACTACACCTGTTCTACCAGAGGCCGCTGCCGC
AGCACTTGCAGCAATGCAGACATTATTTGGTAACCCGAGTAGTAGCCACATTACCGGCTTACAAGCTAAACACTTGATGG
AGCAGACACGCACCAGAGCCCACACTCTACTGGGAACTGGCGAAGGAAAGCTGATCTTTACCAGCGGTGCAACCGAAGGG
ATCCAGACCGCCATTCTATCGGCGCTACTTGCAGCAAAAAAACAGATCCAAACAGGGCAAACCTATAAGCTGCTCTATGG
CGCTACCGAGCACAAAGCCGTGCCTGAGTCGTTAAAACATTGGCTAACCGTGTTAGATATACCGGCGCAGATCATGGCGA
TACCTGTCGATGAGTTTGGGATATTAGATTATGAGTTTATTGCTCAACACGTACCAAACGCATTGATGATCTGCACTATG
GCGGTGAACAATGAAACCGGCGTCTTTCAAGATCTTAAGCAGCTAGAGAAAACCATTCGAGATGCAAACCCCGATACCTT
TTGGATGGTCGACTGCGTGCAGGCTCTGGGTAAAACAGCACTAAACCTAGCGCAAACCAGCATAGATTACGCCCCTTTTA
GTGGTCACAAACTCTATGCCCCGAAAGGTATCGGCTTTGTTTATATTCGTGATGGAGCCCCCTTTACCCCTTTTATAGCC
GGTGGCGGTCAAGAAAGCGGCCTACGCTCTGGCACAGAGAACCTGCCTGGTATGGCGGCGCTTAATGTCATATTTAAGAT
GTTGCAGTGCCCCCAAGACTCAGCCTTTAGCGACCAGCAAACCTTGCAAGGTTATCGACAGCAAATTGCCGACACCTTAA
CCGAGTGCTTTCCCCGCATCGTGTTTAATAACAGCTTTGAACACAGTGTGCCTACGACGATTAACTTTGCCGTACCCAGC
TTTAGCAGCAAAGAGATCATGGACCTGTTCGATGCCGCCAATATTCGTGTCAGCTCTGGCTCAGCCTGCAGCTCTAAGGT
GACCCGCAGTTTTGTGCTCGATGCCATGGGCCTGCCAGCTTGGCAAAGTGAATCGGCTATCAGAATGTCATTTGGCCCAG
CCATGACTCAGGCCGATGTCGATATTGCTTGTGAACGTATCAAAACCGCATCACAAGCCTTAACTCAAAGCTGTATGGTG
TTAGATCGCTCCCGTGATATCGACGATAAGACCCCGCTAGATGGCTTGTTGCAACTTGGTGCTGGCGCCAGCTGCACTTG
GATCTATGTCGATAACAACAGCAAGCAGGCCGTGATTATCGACCCTCTTCCTGAATTACAACATAGACTCGATACACTAC
TGCAATGCCAGCAGCTTGAGGTAAAAGCGATTATTGACACCCACGGGCACGCAGACCATGTATCAGGTCGTGACGTATTA
GCTAACCGCTATTTGGCCAATCAAGATAGCGATATTCTGGGCTGGCCTTATCAGACCCAAACTGTTGAGCATTTAGGCAT
TCAATATGAGTCTATCGAGATTGGCTCACTGCAATTGGTCAAAGTACCGACTCCGGGACATACTGCCGACAGCATTAGCC
TCATTCTGTGCCCATTGAATCTAGGTGATAAGACTCTGCATCAGCAAGCCAAATTCGCTTTCTGTGGCGACACCATCTTG
ATGGGCAGCCTTGGCCGCACTAACTTTGATTCAAGCTCCAGTCAGGCATTATTTCATAGCCTTAAGCTGCTTAATTGCAC
AATAAATCCAGCGACTCTTATCTGTGCCAGCCATGATTACAACCACGAGTTCACCACCACTTTGCCTGCTGAAATGTCGC
GTAACACCTTGCTTAATGCCGTGCTCATGGATCAAGTTTCTGTGGACGATTTTATTGCCCGTAAGGCGAAACTAGACAGC
CACCTTAATGATGAAGTTGGCACAGAGATCATGTGTGGTGCCTACGTAGGGAGCTGCGATAAGTCAGCAATCAAAGAGTA
TGATGCTAAGAGCTTAGCCGCTGCGATAGACAGCAAGGATGAGGTACGGATAATCGACATTCGTGAGCCCCATGAATACG
CGCTTAGTCATCCACAGGAGTCGAGTCAAAACGTGCCTTTAACTCGACTCGTGCAGTTTATCCATGAGCAGCAACATCAA
AAGGATAAGCCTTGGGTGCTAGTTTGCCGCAGTGGTAGTCGCTCTATGGTTGCCGCTCAAGCCATGCACAGATTAGGCTT
TTCACAAATCTCTCATTTGAAAGGCGGCTACGCGTTAAGCCATTAG

Upstream 100 bases:

>100_bases
TTATTCCCAAACTGCTAATACAGCCAACCAGCATTAGTTATACCCAAAAAGAATATATAGTTTTCATTGTAAGTAAAATA
ACAATAAAGGGCCTTACGCC

Downstream 100 bases:

>100_bases
CGTAATGTTTAACAAAGCCATTAGCGTACCTTTAATAAAGCCAGTAGCGCTCATCAAGTGTTGAAATCACAAGCCGCCGA
AGAGTCACTGTTGGCGGCTT

Product: class V aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 761; Mature: 761

Protein sequence:

>761_residues
MHNHNELTQVYLDANATTPVLPEAAAAALAAMQTLFGNPSSSHITGLQAKHLMEQTRTRAHTLLGTGEGKLIFTSGATEG
IQTAILSALLAAKKQIQTGQTYKLLYGATEHKAVPESLKHWLTVLDIPAQIMAIPVDEFGILDYEFIAQHVPNALMICTM
AVNNETGVFQDLKQLEKTIRDANPDTFWMVDCVQALGKTALNLAQTSIDYAPFSGHKLYAPKGIGFVYIRDGAPFTPFIA
GGGQESGLRSGTENLPGMAALNVIFKMLQCPQDSAFSDQQTLQGYRQQIADTLTECFPRIVFNNSFEHSVPTTINFAVPS
FSSKEIMDLFDAANIRVSSGSACSSKVTRSFVLDAMGLPAWQSESAIRMSFGPAMTQADVDIACERIKTASQALTQSCMV
LDRSRDIDDKTPLDGLLQLGAGASCTWIYVDNNSKQAVIIDPLPELQHRLDTLLQCQQLEVKAIIDTHGHADHVSGRDVL
ANRYLANQDSDILGWPYQTQTVEHLGIQYESIEIGSLQLVKVPTPGHTADSISLILCPLNLGDKTLHQQAKFAFCGDTIL
MGSLGRTNFDSSSSQALFHSLKLLNCTINPATLICASHDYNHEFTTTLPAEMSRNTLLNAVLMDQVSVDDFIARKAKLDS
HLNDEVGTEIMCGAYVGSCDKSAIKEYDAKSLAAAIDSKDEVRIIDIREPHEYALSHPQESSQNVPLTRLVQFIHEQQHQ
KDKPWVLVCRSGSRSMVAAQAMHRLGFSQISHLKGGYALSH

Sequences:

>Translated_761_residues
MHNHNELTQVYLDANATTPVLPEAAAAALAAMQTLFGNPSSSHITGLQAKHLMEQTRTRAHTLLGTGEGKLIFTSGATEG
IQTAILSALLAAKKQIQTGQTYKLLYGATEHKAVPESLKHWLTVLDIPAQIMAIPVDEFGILDYEFIAQHVPNALMICTM
AVNNETGVFQDLKQLEKTIRDANPDTFWMVDCVQALGKTALNLAQTSIDYAPFSGHKLYAPKGIGFVYIRDGAPFTPFIA
GGGQESGLRSGTENLPGMAALNVIFKMLQCPQDSAFSDQQTLQGYRQQIADTLTECFPRIVFNNSFEHSVPTTINFAVPS
FSSKEIMDLFDAANIRVSSGSACSSKVTRSFVLDAMGLPAWQSESAIRMSFGPAMTQADVDIACERIKTASQALTQSCMV
LDRSRDIDDKTPLDGLLQLGAGASCTWIYVDNNSKQAVIIDPLPELQHRLDTLLQCQQLEVKAIIDTHGHADHVSGRDVL
ANRYLANQDSDILGWPYQTQTVEHLGIQYESIEIGSLQLVKVPTPGHTADSISLILCPLNLGDKTLHQQAKFAFCGDTIL
MGSLGRTNFDSSSSQALFHSLKLLNCTINPATLICASHDYNHEFTTTLPAEMSRNTLLNAVLMDQVSVDDFIARKAKLDS
HLNDEVGTEIMCGAYVGSCDKSAIKEYDAKSLAAAIDSKDEVRIIDIREPHEYALSHPQESSQNVPLTRLVQFIHEQQHQ
KDKPWVLVCRSGSRSMVAAQAMHRLGFSQISHLKGGYALSH
>Mature_761_residues
MHNHNELTQVYLDANATTPVLPEAAAAALAAMQTLFGNPSSSHITGLQAKHLMEQTRTRAHTLLGTGEGKLIFTSGATEG
IQTAILSALLAAKKQIQTGQTYKLLYGATEHKAVPESLKHWLTVLDIPAQIMAIPVDEFGILDYEFIAQHVPNALMICTM
AVNNETGVFQDLKQLEKTIRDANPDTFWMVDCVQALGKTALNLAQTSIDYAPFSGHKLYAPKGIGFVYIRDGAPFTPFIA
GGGQESGLRSGTENLPGMAALNVIFKMLQCPQDSAFSDQQTLQGYRQQIADTLTECFPRIVFNNSFEHSVPTTINFAVPS
FSSKEIMDLFDAANIRVSSGSACSSKVTRSFVLDAMGLPAWQSESAIRMSFGPAMTQADVDIACERIKTASQALTQSCMV
LDRSRDIDDKTPLDGLLQLGAGASCTWIYVDNNSKQAVIIDPLPELQHRLDTLLQCQQLEVKAIIDTHGHADHVSGRDVL
ANRYLANQDSDILGWPYQTQTVEHLGIQYESIEIGSLQLVKVPTPGHTADSISLILCPLNLGDKTLHQQAKFAFCGDTIL
MGSLGRTNFDSSSSQALFHSLKLLNCTINPATLICASHDYNHEFTTTLPAEMSRNTLLNAVLMDQVSVDDFIARKAKLDS
HLNDEVGTEIMCGAYVGSCDKSAIKEYDAKSLAAAIDSKDEVRIIDIREPHEYALSHPQESSQNVPLTRLVQFIHEQQHQ
KDKPWVLVCRSGSRSMVAAQAMHRLGFSQISHLKGGYALSH

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=389, Percent_Identity=31.8766066838046, Blast_Score=172, Evalue=1e-42,
Organism=Homo sapiens, GI156713448, Length=424, Percent_Identity=29.7169811320755, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI41327741, Length=226, Percent_Identity=27.8761061946903, Blast_Score=81, Evalue=4e-15,
Organism=Escherichia coli, GI48994898, Length=391, Percent_Identity=31.7135549872123, Blast_Score=170, Evalue=4e-43,
Organism=Escherichia coli, GI1789175, Length=409, Percent_Identity=23.960880195599, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=377, Percent_Identity=32.8912466843501, Blast_Score=166, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=25.8461538461538, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17538952, Length=203, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319831, Length=423, Percent_Identity=29.5508274231678, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI20129463, Length=393, Percent_Identity=30.5343511450382, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 83190; Mature: 83190

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNHNELTQVYLDANATTPVLPEAAAAALAAMQTLFGNPSSSHITGLQAKHLMEQTRTRA
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
HTLLGTGEGKLIFTSGATEGIQTAILSALLAAKKQIQTGQTYKLLYGATEHKAVPESLKH
HHEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCHHHHHH
WLTVLDIPAQIMAIPVDEFGILDYEFIAQHVPNALMICTMAVNNETGVFQDLKQLEKTIR
HHHHHCCCCEEEEECCHHCCCCCHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
DANPDTFWMVDCVQALGKTALNLAQTSIDYAPFSGHKLYAPKGIGFVYIRDGAPFTPFIA
CCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCEEE
GGGQESGLRSGTENLPGMAALNVIFKMLQCPQDSAFSDQQTLQGYRQQIADTLTECFPRI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VFNNSFEHSVPTTINFAVPSFSSKEIMDLFDAANIRVSSGSACSSKVTRSFVLDAMGLPA
HCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCC
WQSESAIRMSFGPAMTQADVDIACERIKTASQALTQSCMVLDRSRDIDDKTPLDGLLQLG
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
AGASCTWIYVDNNSKQAVIIDPLPELQHRLDTLLQCQQLEVKAIIDTHGHADHVSGRDVL
CCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHH
ANRYLANQDSDILGWPYQTQTVEHLGIQYESIEIGSLQLVKVPTPGHTADSISLILCPLN
HHHHHCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCEEEEEECCCCCCCCCEEEEEEECC
LGDKTLHQQAKFAFCGDTILMGSLGRTNFDSSSSQALFHSLKLLNCTINPATLICASHDY
CCCHHHHHHHCEEEECCHHEECCCCCCCCCCCCHHHHHHHHHHEEEECCCEEEEEECCCC
NHEFTTTLPAEMSRNTLLNAVLMDQVSVDDFIARKAKLDSHLNDEVGTEIMCGAYVGSCD
CCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCEEEECHHHCCCC
KSAIKEYDAKSLAAAIDSKDEVRIIDIREPHEYALSHPQESSQNVPLTRLVQFIHEQQHQ
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHC
KDKPWVLVCRSGSRSMVAAQAMHRLGFSQISHLKGGYALSH
CCCCEEEEECCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHNHNELTQVYLDANATTPVLPEAAAAALAAMQTLFGNPSSSHITGLQAKHLMEQTRTRA
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
HTLLGTGEGKLIFTSGATEGIQTAILSALLAAKKQIQTGQTYKLLYGATEHKAVPESLKH
HHEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCHHHHHH
WLTVLDIPAQIMAIPVDEFGILDYEFIAQHVPNALMICTMAVNNETGVFQDLKQLEKTIR
HHHHHCCCCEEEEECCHHCCCCCHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
DANPDTFWMVDCVQALGKTALNLAQTSIDYAPFSGHKLYAPKGIGFVYIRDGAPFTPFIA
CCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCEEE
GGGQESGLRSGTENLPGMAALNVIFKMLQCPQDSAFSDQQTLQGYRQQIADTLTECFPRI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VFNNSFEHSVPTTINFAVPSFSSKEIMDLFDAANIRVSSGSACSSKVTRSFVLDAMGLPA
HCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCC
WQSESAIRMSFGPAMTQADVDIACERIKTASQALTQSCMVLDRSRDIDDKTPLDGLLQLG
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
AGASCTWIYVDNNSKQAVIIDPLPELQHRLDTLLQCQQLEVKAIIDTHGHADHVSGRDVL
CCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHH
ANRYLANQDSDILGWPYQTQTVEHLGIQYESIEIGSLQLVKVPTPGHTADSISLILCPLN
HHHHHCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCEEEEEECCCCCCCCCEEEEEEECC
LGDKTLHQQAKFAFCGDTILMGSLGRTNFDSSSSQALFHSLKLLNCTINPATLICASHDY
CCCHHHHHHHCEEEECCHHEECCCCCCCCCCCCHHHHHHHHHHEEEECCCEEEEEECCCC
NHEFTTTLPAEMSRNTLLNAVLMDQVSVDDFIARKAKLDSHLNDEVGTEIMCGAYVGSCD
CCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCEEEECHHHCCCC
KSAIKEYDAKSLAAAIDSKDEVRIIDIREPHEYALSHPQESSQNVPLTRLVQFIHEQQHQ
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHC
KDKPWVLVCRSGSRSMVAAQAMHRLGFSQISHLKGGYALSH
CCCCEEEEECCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]