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The map label for this gene is gdhA [H]

Identifier: 157960356

GI number: 157960356

Start: 643519

End: 644862

Strand: Direct

Name: gdhA [H]

Synonym: Spea_0527

Alternate gene names: 157960356

Gene position: 643519-644862 (Clockwise)

Preceding gene: 157960355

Following gene: 157960357

Centisome position: 12.44

GC content: 48.81

Gene sequence:

>1344_bases
ATGAGCGCAGAGATGACTCTTGATAGTTTTCTTTCTACCGTTGCCGTCCGTAATCCTGACCAACCTGAATTTTTGCAGGC
AGTAAAAGAAGTACTTACTTCTATCTGGCCTTTTGTTGCTGCACACCCTGAGTATTTAGATGCTGGTATTCTTGAACGTC
TTGTTGAGCCTGAGCGCTTAATTCAGTTCCGTGTTTCATGGGTCGATGACCTAGGAAAAGTCCAGGTTAACCGCGCTTTC
CGTATTCAGCACAATTCTGCAATTGGTCCTTTCAAGGGCGGTATGCGTTTCCATCCTTCAGTTAACCAGTCGGTTCTGAA
GTTCTTGGCCTTTGAGCAAACGTTCAAAAACGCACTAACCACATTGCCTATGGGCGGCGGTAAAGGTGGTAGCGACTTCG
ACCCTAAGGGGAAAAGTGATGGCGAAGTGATGCGTTTCTGTCAGGCATTAATGTTCGAGCTGCATCGCCACCTAGGCGCC
GATACGGATGTTCCAGCTGGTGATATTGGTGTTGGTGCCCGTGAAGTGGGCTATATGACAGGCATGATGAAGAAGCTGAC
AAACAGCGCTGAGTGTGTCTTTACAGGTAAAGGCTTGTCTTTCGGTGGTAGTTTGATCCGCCCAGAGGCGACTGGTTACG
GTCTGCTGTACTTCGTCGAAGCAATGCTTAAGGCTAAAGGTGAAACTTTAGAAGGCAAAATCGTTACTGTATCAGGATCG
GGTAACGTAGCTCAGTACGCTATCGAAAAAGCTATGGAGATGGGCGGTAAGGTCATTACTGCATCAGATTCTAGCGGCGT
TGTATTCGATTCTGAAGGTTTTACTCCAGAGAAACTCGCCAAGCTTATGGAAGTTAAAAACGATCGTCGTGGCCGCGTTG
CAGATTACGCCTATGAGATGGGGCTTGAGTATGTGACTGGTGGTACACCTTGGCAGTTTGCTGCAGAAGTAGCTTTGCCT
TGTGCGACTCAGAACGAACTGGATGCCGAGCATGCCAAAATGCTAATCACTAATGGCGTACAGATTGTTGCTGAAGGTGC
CAATATGCCAAGCACCGCTGACGCAATTGCCCTGATGCATGAGGCGAAGGTATTGTTTGCACCGGGTAAGGCTGCCAACG
CCGGTGGTGTTGCGACCTCTGGCCTAGAAATGAGTCAAAACTCATTGCGCTTGAGTTGGAGTCGAGAAGAGGTGGACATG
CGTCTGCGCGAAATCATGTACTCTATCCATGCAACCTGTGAGCAGTATGGCCGTGAAGACACGCACATCAACTATGAGCT
GGGCGCAAACGTAGCAGGCTTTGTAAAAGTAGCTGAGGCCATGAAGGCACAAGGCGTTTACTAA

Upstream 100 bases:

>100_bases
CCATCATTTACTGATATAAAACATTTCACAAAATAAACTGCAGGTGCGCACAGCGCAATCCAAGCAGTAGTAATCTTAAT
TTATACCCTGGAGCACCAAA

Downstream 100 bases:

>100_bases
TATAGATAGTGTGTTTTTAAAAGGGCCTTATGGCCCTTTTTACTTTTGATTCAAACAAGCTATTTTTTATGTGTGCTTAT
ATATGATAACTGAGCGAGAT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSAEMTLDSFLSTVAVRNPDQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERLIQFRVSWVDDLGKVQVNRAF
RIQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGA
DTDVPAGDIGVGAREVGYMTGMMKKLTNSAECVFTGKGLSFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKIVTVSGS
GNVAQYAIEKAMEMGGKVITASDSSGVVFDSEGFTPEKLAKLMEVKNDRRGRVADYAYEMGLEYVTGGTPWQFAAEVALP
CATQNELDAEHAKMLITNGVQIVAEGANMPSTADAIALMHEAKVLFAPGKAANAGGVATSGLEMSQNSLRLSWSREEVDM
RLREIMYSIHATCEQYGREDTHINYELGANVAGFVKVAEAMKAQGVY

Sequences:

>Translated_447_residues
MSAEMTLDSFLSTVAVRNPDQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERLIQFRVSWVDDLGKVQVNRAF
RIQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGA
DTDVPAGDIGVGAREVGYMTGMMKKLTNSAECVFTGKGLSFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKIVTVSGS
GNVAQYAIEKAMEMGGKVITASDSSGVVFDSEGFTPEKLAKLMEVKNDRRGRVADYAYEMGLEYVTGGTPWQFAAEVALP
CATQNELDAEHAKMLITNGVQIVAEGANMPSTADAIALMHEAKVLFAPGKAANAGGVATSGLEMSQNSLRLSWSREEVDM
RLREIMYSIHATCEQYGREDTHINYELGANVAGFVKVAEAMKAQGVY
>Mature_446_residues
SAEMTLDSFLSTVAVRNPDQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERLIQFRVSWVDDLGKVQVNRAFR
IQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGAD
TDVPAGDIGVGAREVGYMTGMMKKLTNSAECVFTGKGLSFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKIVTVSGSG
NVAQYAIEKAMEMGGKVITASDSSGVVFDSEGFTPEKLAKLMEVKNDRRGRVADYAYEMGLEYVTGGTPWQFAAEVALPC
ATQNELDAEHAKMLITNGVQIVAEGANMPSTADAIALMHEAKVLFAPGKAANAGGVATSGLEMSQNSLRLSWSREEVDMR
LREIMYSIHATCEQYGREDTHINYELGANVAGFVKVAEAMKAQGVY

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=363, Percent_Identity=28.6501377410468, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4885281, Length=363, Percent_Identity=28.3746556473829, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1788059, Length=446, Percent_Identity=70.1793721973094, Blast_Score=652, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=367, Percent_Identity=29.1553133514986, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324951, Length=451, Percent_Identity=49.6674057649667, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319256, Length=453, Percent_Identity=49.4481236203091, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45549226, Length=383, Percent_Identity=28.1984334203655, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45553475, Length=363, Percent_Identity=28.3746556473829, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24649283, Length=362, Percent_Identity=29.0055248618785, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24649460, Length=318, Percent_Identity=28.3018867924528, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48458; Mature: 48327

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEMTLDSFLSTVAVRNPDQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERL
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHH
IQFRVSWVDDLGKVQVNRAFRIQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALT
HHHHHHHHHHHCCEEECEEEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGADTDVPAGDIGVGAREVGYMT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
GMMKKLTNSAECVFTGKGLSFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKIVTVSGS
HHHHHHCCCCCEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECC
GNVAQYAIEKAMEMGGKVITASDSSGVVFDSEGFTPEKLAKLMEVKNDRRGRVADYAYEM
CCHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
GLEYVTGGTPWQFAAEVALPCATQNELDAEHAKMLITNGVQIVAEGANMPSTADAIALMH
CCCEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
EAKVLFAPGKAANAGGVATSGLEMSQNSLRLSWSREEVDMRLREIMYSIHATCEQYGRED
HCEEEECCCCCCCCCCCCCCCCHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCC
THINYELGANVAGFVKVAEAMKAQGVY
CEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAEMTLDSFLSTVAVRNPDQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERL
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHH
IQFRVSWVDDLGKVQVNRAFRIQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALT
HHHHHHHHHHHCCEEECEEEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGADTDVPAGDIGVGAREVGYMT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
GMMKKLTNSAECVFTGKGLSFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKIVTVSGS
HHHHHHCCCCCEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECC
GNVAQYAIEKAMEMGGKVITASDSSGVVFDSEGFTPEKLAKLMEVKNDRRGRVADYAYEM
CCHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
GLEYVTGGTPWQFAAEVALPCATQNELDAEHAKMLITNGVQIVAEGANMPSTADAIALMH
CCCEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
EAKVLFAPGKAANAGGVATSGLEMSQNSLRLSWSREEVDMRLREIMYSIHATCEQYGRED
HCEEEECCCCCCCCCCCCCCCCHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCC
THINYELGANVAGFVKVAEAMKAQGVY
CEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]