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The map label for this gene is rbsB [H]

Identifier: 157960346

GI number: 157960346

Start: 631445

End: 632344

Strand: Direct

Name: rbsB [H]

Synonym: Spea_0517

Alternate gene names: 157960346

Gene position: 631445-632344 (Clockwise)

Preceding gene: 157960345

Following gene: 157960347

Centisome position: 12.2

GC content: 49.67

Gene sequence:

>900_bases
ATGAAGCGACTTGCCCATCACAAAGTGTCAGTTCTCTTGTCTGCAGCCTTGCTGTCGGTTAGCACGTCTTTCAGCGCCAG
CGCGCAAGATGCGATAGCTATCGTGCTATCGACGATGAACAACCCGTTTTTTGTCACCATGAAAGATGGCGCCGAGGCTA
AAGCGGCAGAGCTTGGCTACAAGCTAATAGCGCTGGACTCGCAAAACGATCCCAGCAAAGAGCTGGCAAATGTTGAAGAT
CTCACCATGCGCGGCGTTAAGGCGATTTTGATTAATCCAACCGATGCATTAGCGGTAACCAATGCGATTCGCACCGCTAA
TCGTGCCAACATTCCGGTACTGACATTAGATAGAACCGCAGCACATGGCGATATCGTCAGCCATATTGCATCCGACAATA
TCGCTGGTGGAGAACTTGCTGGGGAGTTTATTGCACAGCAACTAGGCAAGAACGCTAAAGTTATTCAGCTTGAGGGCATA
GCCGGTACATCGGCCGCCCGTGAGCGCGGTGAAGGTTTTAGTAACGCAGTGACGGCCAATAACTTTGAGTTGTTATCTAG
CCAGCCTGCGGATTTTGACCGCTCAAAAGGCTTAAACGTGATGGAAAATATGCTGGCAGCCAATGGCGATGTGCAGGCGG
TATTTGCCCAGAATGATGAGATGGCGTTAGGCGCACTGCGCGCAATTGAGGCGGCGGGTAAAGATATTCTGGTGATTGGC
TTTGATGGCACCGATGAAGGTATTGCGGCGGTTAGACGTCAGCAACTGTCGGCAACGGTTGCTCAGCAACCTGAGCTATT
AGGTGCGACGGCGATTATGACAGCAGATAGAATTTTAAAAGGCCAAGTCGTTGAAAAATCTATCCCTGTTGCGTTAAAAA
TCATTACTCAAACACAGTAA

Upstream 100 bases:

>100_bases
AACAAAAAGCCTAGACTCTTTATCGGCAGAGCTTAGCTAACACTCTAACTAGAGTGATATGAGTTCTGCCTTTGCATTTA
CTGTATTGAAAAGGTTTACC

Downstream 100 bases:

>100_bases
TCTTACTGATAATTTGTCAAACTAGTATGCACGTCAATTGAATTGTCATCATTGGCGTATTTTCCCTCTGAGCCCAACAT
CATTGCGAGTTCGGTTATTG

Product: D-ribose transporter subunit RbsB

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKRLAHHKVSVLLSAALLSVSTSFSASAQDAIAIVLSTMNNPFFVTMKDGAEAKAAELGYKLIALDSQNDPSKELANVED
LTMRGVKAILINPTDALAVTNAIRTANRANIPVLTLDRTAAHGDIVSHIASDNIAGGELAGEFIAQQLGKNAKVIQLEGI
AGTSAARERGEGFSNAVTANNFELLSSQPADFDRSKGLNVMENMLAANGDVQAVFAQNDEMALGALRAIEAAGKDILVIG
FDGTDEGIAAVRRQQLSATVAQQPELLGATAIMTADRILKGQVVEKSIPVALKIITQTQ

Sequences:

>Translated_299_residues
MKRLAHHKVSVLLSAALLSVSTSFSASAQDAIAIVLSTMNNPFFVTMKDGAEAKAAELGYKLIALDSQNDPSKELANVED
LTMRGVKAILINPTDALAVTNAIRTANRANIPVLTLDRTAAHGDIVSHIASDNIAGGELAGEFIAQQLGKNAKVIQLEGI
AGTSAARERGEGFSNAVTANNFELLSSQPADFDRSKGLNVMENMLAANGDVQAVFAQNDEMALGALRAIEAAGKDILVIG
FDGTDEGIAAVRRQQLSATVAQQPELLGATAIMTADRILKGQVVEKSIPVALKIITQTQ
>Mature_299_residues
MKRLAHHKVSVLLSAALLSVSTSFSASAQDAIAIVLSTMNNPFFVTMKDGAEAKAAELGYKLIALDSQNDPSKELANVED
LTMRGVKAILINPTDALAVTNAIRTANRANIPVLTLDRTAAHGDIVSHIASDNIAGGELAGEFIAQQLGKNAKVIQLEGI
AGTSAARERGEGFSNAVTANNFELLSSQPADFDRSKGLNVMENMLAANGDVQAVFAQNDEMALGALRAIEAAGKDILVIG
FDGTDEGIAAVRRQQLSATVAQQPELLGATAIMTADRILKGQVVEKSIPVALKIITQTQ

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=291, Percent_Identity=61.5120274914089, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1790526, Length=292, Percent_Identity=33.9041095890411, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790674, Length=230, Percent_Identity=34.3478260869565, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1790194, Length=271, Percent_Identity=26.1992619926199, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787948, Length=291, Percent_Identity=24.7422680412371, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788473, Length=277, Percent_Identity=27.4368231046931, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786540, Length=233, Percent_Identity=30.9012875536481, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 31357; Mature: 31357

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLAHHKVSVLLSAALLSVSTSFSASAQDAIAIVLSTMNNPFFVTMKDGAEAKAAELGY
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCEEEEECCCCCCHHHHCCE
KLIALDSQNDPSKELANVEDLTMRGVKAILINPTDALAVTNAIRTANRANIPVLTLDRTA
EEEEEECCCCCHHHHCCHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCC
AHGDIVSHIASDNIAGGELAGEFIAQQLGKNAKVIQLEGIAGTSAARERGEGFSNAVTAN
CHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCEECC
NFELLSSQPADFDRSKGLNVMENMLAANGDVQAVFAQNDEMALGALRAIEAAGKDILVIG
CCCEECCCCCCCCHHCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEE
FDGTDEGIAAVRRQQLSATVAQQPELLGATAIMTADRILKGQVVEKSIPVALKIITQTQ
ECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEECCC
>Mature Secondary Structure
MKRLAHHKVSVLLSAALLSVSTSFSASAQDAIAIVLSTMNNPFFVTMKDGAEAKAAELGY
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCEEEEECCCCCCHHHHCCE
KLIALDSQNDPSKELANVEDLTMRGVKAILINPTDALAVTNAIRTANRANIPVLTLDRTA
EEEEEECCCCCHHHHCCHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCC
AHGDIVSHIASDNIAGGELAGEFIAQQLGKNAKVIQLEGIAGTSAARERGEGFSNAVTAN
CHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCEECC
NFELLSSQPADFDRSKGLNVMENMLAANGDVQAVFAQNDEMALGALRAIEAAGKDILVIG
CCCEECCCCCCCCHHCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEE
FDGTDEGIAAVRRQQLSATVAQQPELLGATAIMTADRILKGQVVEKSIPVALKIITQTQ
ECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]