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The map label for this gene is nrfC [H]

Identifier: 157960312

GI number: 157960312

Start: 594264

End: 594971

Strand: Direct

Name: nrfC [H]

Synonym: Spea_0483

Alternate gene names: 157960312

Gene position: 594264-594971 (Clockwise)

Preceding gene: 157960311

Following gene: 157960313

Centisome position: 11.48

GC content: 47.74

Gene sequence:

>708_bases
GTGAGTGAAAATCTAGAGAGACGGAGGTTCCTTAAATGTCTAGGAGCCAGCTCTTTGATATTGGCACCGCTAGGGTGTAG
CTCGGTCAAAGACAAGGAAGATACCAACAAGCCGCACTATGTAATGGTTTTCGACCAAAACAAATGTGTTGGTTGCGGTG
AGTGTAAGGATGCTTGTAATAAAGCTAACCACTTACCAGAAGGTAAATCTCGCCTATTAATGGAAGAGCAATCAAGCGCC
GTCGAGGGTCAACCTTGTCCACACTGTGGTAAAACCGGTGACTGTGGTTGTCAGCGTAAGTTTGTGCGTGTTTCATGTCA
GCAATGTAAGAATGCTCCTTGCGTAACAGTTTGCCCAACAGGCGCTGCACATCGTGATGAGAAGACCGGCATTGTGACTA
TGGATGCGGCGAAATGTGCTGGTTGTAAGTACTGTATCGGTGCTTGCCCATATGACGCACGTTTCATCAATAAAGAAACT
GACGTCGCCGATAACTGTGACTTCTGCCTAAACAGCAAACTTGCCATCGGCGAGTTACCTGCTTGTGTGCAGCAGTGCCG
TTACGACGCGCTGATCTTTGGTGATGCAAATGATCCAACTTCATACGTGAGTAAGCTATTGGCAGTGAAGGACTCGGTAC
GTATTAAGCCGGGCTTCGGTACTGAGCCTAGCCTGCGTTACATCCCTATTGTGAAGCTAGGAGTATAG

Upstream 100 bases:

>100_bases
TTGATGCCGATGTTGCTCATTAACGCTTAAGTAAACGCCAGACAGAATATTAAATTAAAAAAATAAAGTGTCGGTTTTAA
ACCGATAATCGGAGGATGCT

Downstream 100 bases:

>100_bases
TCATGGACGGTAATATTGAATTTACAATGGGCCTGTCACAAGGCATCGCTTGGCCTTGGCCGATTGCGGTTTACCTGTTC
TTTGCTGGTATCTCTGGTGG

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSENLERRRFLKCLGASSLILAPLGCSSVKDKEDTNKPHYVMVFDQNKCVGCGECKDACNKANHLPEGKSRLLMEEQSSA
VEGQPCPHCGKTGDCGCQRKFVRVSCQQCKNAPCVTVCPTGAAHRDEKTGIVTMDAAKCAGCKYCIGACPYDARFINKET
DVADNCDFCLNSKLAIGELPACVQQCRYDALIFGDANDPTSYVSKLLAVKDSVRIKPGFGTEPSLRYIPIVKLGV

Sequences:

>Translated_235_residues
MSENLERRRFLKCLGASSLILAPLGCSSVKDKEDTNKPHYVMVFDQNKCVGCGECKDACNKANHLPEGKSRLLMEEQSSA
VEGQPCPHCGKTGDCGCQRKFVRVSCQQCKNAPCVTVCPTGAAHRDEKTGIVTMDAAKCAGCKYCIGACPYDARFINKET
DVADNCDFCLNSKLAIGELPACVQQCRYDALIFGDANDPTSYVSKLLAVKDSVRIKPGFGTEPSLRYIPIVKLGV
>Mature_234_residues
SENLERRRFLKCLGASSLILAPLGCSSVKDKEDTNKPHYVMVFDQNKCVGCGECKDACNKANHLPEGKSRLLMEEQSSAV
EGQPCPHCGKTGDCGCQRKFVRVSCQQCKNAPCVTVCPTGAAHRDEKTGIVTMDAAKCAGCKYCIGACPYDARFINKETD
VADNCDFCLNSKLAIGELPACVQQCRYDALIFGDANDPTSYVSKLLAVKDSVRIKPGFGTEPSLRYIPIVKLGV

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=227, Percent_Identity=42.2907488986784, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI226510944, Length=198, Percent_Identity=42.4242424242424, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1789370, Length=227, Percent_Identity=30.3964757709251, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787872, Length=201, Percent_Identity=31.3432835820896, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787122, Length=201, Percent_Identity=30.3482587064677, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787749, Length=162, Percent_Identity=27.1604938271605, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI2367245, Length=152, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787963, Length=178, Percent_Identity=29.7752808988764, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787740, Length=96, Percent_Identity=34.375, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787478, Length=96, Percent_Identity=34.375, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1790326, Length=170, Percent_Identity=25.8823529411765, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25490; Mature: 25358

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
11.1 %Cys+Met (Translated Protein)
9.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
10.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENLERRRFLKCLGASSLILAPLGCSSVKDKEDTNKPHYVMVFDQNKCVGCGECKDACN
CCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
KANHLPEGKSRLLMEEQSSAVEGQPCPHCGKTGDCGCQRKFVRVSCQQCKNAPCVTVCPT
HHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
GAAHRDEKTGIVTMDAAKCAGCKYCIGACPYDARFINKETDVADNCDFCLNSKLAIGELP
CCCCCCCCCCEEEECCHHHCCCCCEECCCCCCCEECCCCCCCCCCCCHHHCCCCCCCHHH
ACVQQCRYDALIFGDANDPTSYVSKLLAVKDSVRIKPGFGTEPSLRYIPIVKLGV
HHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEECCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
SENLERRRFLKCLGASSLILAPLGCSSVKDKEDTNKPHYVMVFDQNKCVGCGECKDACN
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
KANHLPEGKSRLLMEEQSSAVEGQPCPHCGKTGDCGCQRKFVRVSCQQCKNAPCVTVCPT
HHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
GAAHRDEKTGIVTMDAAKCAGCKYCIGACPYDARFINKETDVADNCDFCLNSKLAIGELP
CCCCCCCCCCEEEECCHHHCCCCCEECCCCCCCEECCCCCCCCCCCCHHHCCCCCCCHHH
ACVQQCRYDALIFGDANDPTSYVSKLLAVKDSVRIKPGFGTEPSLRYIPIVKLGV
HHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEECCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]