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The map label for this gene is uvrD [H]

Identifier: 157960297

GI number: 157960297

Start: 576841

End: 579006

Strand: Direct

Name: uvrD [H]

Synonym: Spea_0468

Alternate gene names: 157960297

Gene position: 576841-579006 (Clockwise)

Preceding gene: 157960296

Following gene: 157960298

Centisome position: 11.15

GC content: 49.4

Gene sequence:

>2166_bases
ATGGACGTATCTTCTTTACTAGACGGCCTAAACGACAAGCAGCGAGAAGCTGTTGGTGCACCGCAATCCAGCATGTTGGT
ATTGGCGGGTGCGGGTAGTGGCAAAACGCGGGTGCTCACCCACAGAATTGCCTGGTTGATGCAGGTTGAACAGCAAAGCC
CTTATGCAATTTTAGCGGTAACCTTTACCAATAAAGCGGCAAAAGAGATGCGTGAGCGTGTTGAAAAAGTTGCTGGTGGC
AATATGAGCCGCATGTGGATCGGTACTTTCCACGGCTTAGCCCACCGCTTGCTGCGTACCCATTATAAAGATGCCAATCT
GCCTGAGAGCTTCCAGATCTTAGATTCAGACGATCAACTGCGTCTGATCAAACGTATTCTAAAAAGCCTTAACTTGGACG
AGAAGCAGTATCCGCCAAGACAAGCTCAAGGCTACATCAATGGTAAGAAAGACTTAGGTCTACGCCCGTCGCATATCGAT
GCGGGCGGCTTCCCGATTGAGCAAAATTTACTGAAGATCTATCAGATCTATCAAGATTCATGCGATCGCGCAGGTTTAGT
CGATTTTGCGGAAATCTTACTCAGAGCCCATGAGCTTTGGCTCAACAAGCCGCATATTCTGGATCATTACCAAGACAGGT
TTAAGCATATTCTGGTAGACGAGTTCCAAGACACTAACGCCATTCAATACGCATGGATCCGTGTACTTGCGGGTTCTACT
GCCAATGTGATGATAGTCGGTGATGACGACCAGTCTATCTACGGCTGGCGCGGCGCTCAGGTGGAGAACTTGCATAAGTT
CTTGGCCGACTTCCCAGCGGCGCAAACTATTCGTTTGGAACAGAACTATCGTTCGAGCGGTAATATTCTAAATGCCTCGA
ACGAGCTGATTGCCAATAACCCAGATCGTATGGGTAAAAAACTTTGGACCGACGACAAAGACGGTGAGCCAATTGGCCTT
TACTGTGCCTTTAACGAAATGGATGAGGCGCGCTTTATCGTTGGCCGCATTACCGATTGGCAAGATGAGGGGGGCAAGTT
AGAAGATTGCGCCATTCTTTATCGTTCCAACGCTCAGTCCCGCGTACTGGAAGAAGCCTTATTGCATAAAGGCATCGCTT
ATCGTATCTATGGCGGCCTGCGCTTCTTCGAACGCCAAGAGATTAAAGACGCCATGGGTTACCTGCGACTTATCAATAAC
AAGAATGATGATGCTGCCTTTGAGCGTGTGGTGAATACCCCAACTCGTGGTATTGGCGATCGCACTCTAGATATCATTCG
TACCACTGCACGCCAGCAAGAGATGACCCTGTGGGAAGCGAGTCTTCGCTTACTGGAGGAGAAGGTCCTTAACGGCCGTG
CCGCCAATGCCGTACGGGGTTTTATGGACTTGATTGTCGCCATGAGAATGGACACCGACGAGCAATCTTTGCACCGTATG
ACAGACCATGTGATCCAAACATCGGGTCTAAAGGCGATGTATGAGGCCGAGAAGAGTGAAAAGGCCCGTGCACGTGTAGA
GAACTTAGAAGAATTAGTTACAGCTGCTCGCAGCTTTGTGATGCCTGAAGAGATCGAAGACATGGGCGAGCTCAATGCCT
TCCTGTCTCACGCAGCGCTAGAAGCGGGTGAAGGCCAGGCTGATGAGTTTACCGATGCGGTGCAGTTAATGACACTACAC
TCGGCAAAGGGTCTTGAGTTCCCAATGGTGTTTATGGCGGGTGTCGAAGAAGGCTTGTTCCCGAGCCAGATGGCTATCGA
TGAAGGTGACCGTCTCGATGAGGAGCGCCGTCTCTGTTATGTGGGTATGACCCGTGCCATGCAGAAGCTGTATATCACCT
ACGCCGAGTCGAGACGCATTTATGGCCGTGAAAGCTTCGCGAGACCTTCACGTTTTGTAAAGGAAATTCCTGAGCAATAT
GTGCAGGAGATTCGTATGCGTACTCAGGTTTCAGCGCCAGCAGTCAGCAGTCGCTTTAGTAAGCAAGATAATGCCTTCAA
TGATTCAGGCTTTAAGATTGGTCAGCGTGTGATGCACCCTAAATTTGGTGAGGGCACTGTGACCAATTATGAAGGAAGTG
GTGCACAGGCGCGGATTCAGGTTAACTTTTTCGATGTTGGTAGTAAGTGGTTAGTCATCGCTTACGCGCGCTTGGAATCG
TTATAA

Upstream 100 bases:

>100_bases
GATTCCATTTCTTAACGATTTCCCCCTGCTAGTTTCGCTTATTCTATCCCCGAGAACCTCGCTTCTGTTATACTGCCCGC
CTGATTTTTTGGAGGCAATA

Downstream 100 bases:

>100_bases
TCGTGATGGTTGAATAAGGCTGAGCTTAGACTCAGCCTTATTTTTAGCCGAAAAAAAACACGCTGTTCTTATTTATCTCG
ATACTGGTTGAATACTGTTA

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MDVSSLLDGLNDKQREAVGAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYAILAVTFTNKAAKEMRERVEKVAGG
NMSRMWIGTFHGLAHRLLRTHYKDANLPESFQILDSDDQLRLIKRILKSLNLDEKQYPPRQAQGYINGKKDLGLRPSHID
AGGFPIEQNLLKIYQIYQDSCDRAGLVDFAEILLRAHELWLNKPHILDHYQDRFKHILVDEFQDTNAIQYAWIRVLAGST
ANVMIVGDDDQSIYGWRGAQVENLHKFLADFPAAQTIRLEQNYRSSGNILNASNELIANNPDRMGKKLWTDDKDGEPIGL
YCAFNEMDEARFIVGRITDWQDEGGKLEDCAILYRSNAQSRVLEEALLHKGIAYRIYGGLRFFERQEIKDAMGYLRLINN
KNDDAAFERVVNTPTRGIGDRTLDIIRTTARQQEMTLWEASLRLLEEKVLNGRAANAVRGFMDLIVAMRMDTDEQSLHRM
TDHVIQTSGLKAMYEAEKSEKARARVENLEELVTAARSFVMPEEIEDMGELNAFLSHAALEAGEGQADEFTDAVQLMTLH
SAKGLEFPMVFMAGVEEGLFPSQMAIDEGDRLDEERRLCYVGMTRAMQKLYITYAESRRIYGRESFARPSRFVKEIPEQY
VQEIRMRTQVSAPAVSSRFSKQDNAFNDSGFKIGQRVMHPKFGEGTVTNYEGSGAQARIQVNFFDVGSKWLVIAYARLES
L

Sequences:

>Translated_721_residues
MDVSSLLDGLNDKQREAVGAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYAILAVTFTNKAAKEMRERVEKVAGG
NMSRMWIGTFHGLAHRLLRTHYKDANLPESFQILDSDDQLRLIKRILKSLNLDEKQYPPRQAQGYINGKKDLGLRPSHID
AGGFPIEQNLLKIYQIYQDSCDRAGLVDFAEILLRAHELWLNKPHILDHYQDRFKHILVDEFQDTNAIQYAWIRVLAGST
ANVMIVGDDDQSIYGWRGAQVENLHKFLADFPAAQTIRLEQNYRSSGNILNASNELIANNPDRMGKKLWTDDKDGEPIGL
YCAFNEMDEARFIVGRITDWQDEGGKLEDCAILYRSNAQSRVLEEALLHKGIAYRIYGGLRFFERQEIKDAMGYLRLINN
KNDDAAFERVVNTPTRGIGDRTLDIIRTTARQQEMTLWEASLRLLEEKVLNGRAANAVRGFMDLIVAMRMDTDEQSLHRM
TDHVIQTSGLKAMYEAEKSEKARARVENLEELVTAARSFVMPEEIEDMGELNAFLSHAALEAGEGQADEFTDAVQLMTLH
SAKGLEFPMVFMAGVEEGLFPSQMAIDEGDRLDEERRLCYVGMTRAMQKLYITYAESRRIYGRESFARPSRFVKEIPEQY
VQEIRMRTQVSAPAVSSRFSKQDNAFNDSGFKIGQRVMHPKFGEGTVTNYEGSGAQARIQVNFFDVGSKWLVIAYARLES
L
>Mature_721_residues
MDVSSLLDGLNDKQREAVGAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYAILAVTFTNKAAKEMRERVEKVAGG
NMSRMWIGTFHGLAHRLLRTHYKDANLPESFQILDSDDQLRLIKRILKSLNLDEKQYPPRQAQGYINGKKDLGLRPSHID
AGGFPIEQNLLKIYQIYQDSCDRAGLVDFAEILLRAHELWLNKPHILDHYQDRFKHILVDEFQDTNAIQYAWIRVLAGST
ANVMIVGDDDQSIYGWRGAQVENLHKFLADFPAAQTIRLEQNYRSSGNILNASNELIANNPDRMGKKLWTDDKDGEPIGL
YCAFNEMDEARFIVGRITDWQDEGGKLEDCAILYRSNAQSRVLEEALLHKGIAYRIYGGLRFFERQEIKDAMGYLRLINN
KNDDAAFERVVNTPTRGIGDRTLDIIRTTARQQEMTLWEASLRLLEEKVLNGRAANAVRGFMDLIVAMRMDTDEQSLHRM
TDHVIQTSGLKAMYEAEKSEKARARVENLEELVTAARSFVMPEEIEDMGELNAFLSHAALEAGEGQADEFTDAVQLMTLH
SAKGLEFPMVFMAGVEEGLFPSQMAIDEGDRLDEERRLCYVGMTRAMQKLYITYAESRRIYGRESFARPSRFVKEIPEQY
VQEIRMRTQVSAPAVSSRFSKQDNAFNDSGFKIGQRVMHPKFGEGTVTNYEGSGAQARIQVNFFDVGSKWLVIAYARLES
L

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=721, Percent_Identity=69.7642163661581, Blast_Score=1060, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=635, Percent_Identity=38.8976377952756, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1787196, Length=91, Percent_Identity=43.956043956044, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322369, Length=757, Percent_Identity=28.1373844121532, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6324477, Length=687, Percent_Identity=22.8529839883552, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81854; Mature: 81854

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSSLLDGLNDKQREAVGAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYAILAV
CCHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
TFTNKAAKEMRERVEKVAGGNMSRMWIGTFHGLAHRLLRTHYKDANLPESFQILDSDDQL
EECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHH
RLIKRILKSLNLDEKQYPPRQAQGYINGKKDLGLRPSHIDAGGFPIEQNLLKIYQIYQDS
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
CDRAGLVDFAEILLRAHELWLNKPHILDHYQDRFKHILVDEFQDTNAIQYAWIRVLAGST
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
ANVMIVGDDDQSIYGWRGAQVENLHKFLADFPAAQTIRLEQNYRSSGNILNASNELIANN
CEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHCCC
PDRMGKKLWTDDKDGEPIGLYCAFNEMDEARFIVGRITDWQDEGGKLEDCAILYRSNAQS
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCHHEEEECCCCHH
RVLEEALLHKGIAYRIYGGLRFFERQEIKDAMGYLRLINNKNDDAAFERVVNTPTRGIGD
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
RTLDIIRTTARQQEMTLWEASLRLLEEKVLNGRAANAVRGFMDLIVAMRMDTDEQSLHRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
TDHVIQTSGLKAMYEAEKSEKARARVENLEELVTAARSFVMPEEIEDMGELNAFLSHAAL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EAGEGQADEFTDAVQLMTLHSAKGLEFPMVFMAGVEEGLFPSQMAIDEGDRLDEERRLCY
HCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHEEH
VGMTRAMQKLYITYAESRRIYGRESFARPSRFVKEIPEQYVQEIRMRTQVSAPAVSSRFS
HHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
KQDNAFNDSGFKIGQRVMHPKFGEGTVTNYEGSGAQARIQVNFFDVGSKWLVIAYARLES
CCCCCCCCCCHHHHHHHCCCCCCCCCEECCCCCCCEEEEEEEEEECCCCEEEEEHHHHHC
L
C
>Mature Secondary Structure
MDVSSLLDGLNDKQREAVGAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYAILAV
CCHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
TFTNKAAKEMRERVEKVAGGNMSRMWIGTFHGLAHRLLRTHYKDANLPESFQILDSDDQL
EECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHH
RLIKRILKSLNLDEKQYPPRQAQGYINGKKDLGLRPSHIDAGGFPIEQNLLKIYQIYQDS
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
CDRAGLVDFAEILLRAHELWLNKPHILDHYQDRFKHILVDEFQDTNAIQYAWIRVLAGST
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
ANVMIVGDDDQSIYGWRGAQVENLHKFLADFPAAQTIRLEQNYRSSGNILNASNELIANN
CEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHCCC
PDRMGKKLWTDDKDGEPIGLYCAFNEMDEARFIVGRITDWQDEGGKLEDCAILYRSNAQS
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCHHEEEECCCCHH
RVLEEALLHKGIAYRIYGGLRFFERQEIKDAMGYLRLINNKNDDAAFERVVNTPTRGIGD
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
RTLDIIRTTARQQEMTLWEASLRLLEEKVLNGRAANAVRGFMDLIVAMRMDTDEQSLHRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
TDHVIQTSGLKAMYEAEKSEKARARVENLEELVTAARSFVMPEEIEDMGELNAFLSHAAL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EAGEGQADEFTDAVQLMTLHSAKGLEFPMVFMAGVEEGLFPSQMAIDEGDRLDEERRLCY
HCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHEEH
VGMTRAMQKLYITYAESRRIYGRESFARPSRFVKEIPEQYVQEIRMRTQVSAPAVSSRFS
HHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
KQDNAFNDSGFKIGQRVMHPKFGEGTVTNYEGSGAQARIQVNFFDVGSKWLVIAYARLES
CCCCCCCCCCHHHHHHHCCCCCCCCCEECCCCCCCEEEEEEEEEECCCCEEEEEHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]