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The map label for this gene is selB [H]

Identifier: 157960272

GI number: 157960272

Start: 550745

End: 552652

Strand: Direct

Name: selB [H]

Synonym: Spea_0443

Alternate gene names: 157960272

Gene position: 550745-552652 (Clockwise)

Preceding gene: 157960271

Following gene: 157960273

Centisome position: 10.64

GC content: 48.48

Gene sequence:

>1908_bases
ATGATTGTTGTTACTTCAGGCCATGTGGACCACGGTAAAACCAGCCTGATTCAAGCGCTAACTGGCACCGATGCCGACCG
CTTACCGGAAGAAAAACAGCGTGGCATGACCATAGAGCTTGGCTACGCCTTTATGGATTTATCAGATGGTGAGCGCCTAG
CTTTTGTCGATGTGCCGGGCCACAGCAAATTTATTAATACCATGCTGGCTGGGGTGAGCTGCGCCAAACATGCGCTATTG
ATCATCGCTTGTGATGATGGCGTGATGCCGCAAACTTATGAGCATTTAGCCATCTTGCAGCTGCTTAATCTTGAACACTT
AATCGTGGTGCTAACCAAGCAAGACAAGGTCGATGCCACCAGAGTCGATGAAGTTAAAGAGCAAGTGAGTGAGCTATTGG
CGCAGCACCAAAGACAAAGCCCAATCTTTGTGGTGTCGGCGCAATCTGGTTTTGGTATCGACGCGCTTAAGCTTGCTATC
ACTGAGCTTGCCGAGTCACAGATAACAGCGTTAAGCGAGACCGGCGAGCAAGCCAGCTTTCGTATGGCAATCGACAGAGC
TTTTAGCGTAAAAGGTGCAGGTCTTGTGGTAACTGGTACTGTGATTAGTGGTCAAGTCAGCCAAGGGCAAAGCTTGTATC
TATCAGGCCAGCGTAAACCAGTAAAAGTCAGAACCCTTCACAGTCAAGGACGGGTAAGTAATAGCGCGCATCATTGTCAG
CGGGTGGCGCTCAATATTACTGGAGTCGACCAACATCGCGGCGCAGAGCGCGGAGATTGGTTAACTGCGCTAGCGCCAGC
GGCCGAAGCGCCGAACGTGATTTGTGGCGTATTTGAATCTTGCCTGCCGTTTAAGCATTGGCAAAATGTGCAAGTGCATT
TAGGTAATCGCCATACCACGGCGCGCTTAGGCTTACTGCAAAACCTTGAAGATGGCACGGCCTTGGTTGAGCTGCAATGC
GAGCAGCCCCTTCATAGTGTGGAGCAAGACAGAATCATCGTGCGTGACAGCGCCGCTAAAAGCACATTAGGTGCGCTGCG
GGTATTAGACATTAACCCGCCAACTCGAGCAAAGCGCAGTGTTGAGCGCTTAGCTTACCTAGAAAACCTTAAACAGTGCG
ACTCACTAACGGCCAGTTTGCAAGTTCAAATAAACCATCACTGCGTTCCAAAGTCCGCTTTCATGTGGGCTAATCAGCTA
ACAGAGCAAACCGCTTGTTTGGCGGACAAACAGGTGATCAGCCTCGATGGTTATTTGTTATCCAGCATATTACAAGAGCA
GATTGAAGCGCAGTTGCTGGCACTCATCGCCGATTATCATCAGCAGTCGCCAGAGCTGTTAGGGTTGAGTATTAATCGAT
TGCAGCGTATGACTCACAACCAATATCCATTGCCTGTGCTACTTAAACTTACCGATGGTCTCAGAGAGCAAAAACTACTG
CAAAGTACCCGTGGCTTATTGCATCTGCCACGGCATAACTTGGCGCTGTCAGACGCTGAGCTACAGCTGTGGTCACAGAT
AAAGCAGCAGATGCTTAGTGCTAACGGGCCATTATGGTCATCAGACTTAGCATCAACGCTAAACCTTGAGTTGACTACAG
TGCGGGCGCTATGCCGTAAGCTTATCCAGCTGGGGTATCTGTCGGCGATTATTAAAGACAGATTTATGCTCACCGAAAGC
CTGTATTTCTACGCCGATGTCATTCGTAATCAAATCAATGTGAGTGGTGAAATCAAGACCGCCGATTTTAATTCAATCAT
TAAGCTTGGCCGCAAAGCCAGTATTCAGTTGTTAGAATTTTATGACCGCAGTGGATTAACGTGGCGCAAGAAGAAAGACA
ATTCGAGAGTGATTAGAGATGGCAGCATTTTTATAGCAAGTGATTTGGCGGAAGAACATAGGAGTTGA

Upstream 100 bases:

>100_bases
ACGATACCCAGCTTATCGAAGTCCTGACAAAAACCTTAATCGAGTTTGAGCAAGCTCATATCAACACGCTTTCCGTATTA
CCAAGAGTAGAGGCTCAGTC

Downstream 100 bases:

>100_bases
ACCTACCCGGGACTGCTGGCAGCCCCACCTGGATTTGAAATCCAGACGTTTCACCGGAAACGACGTTCTTCCTTATCACT
TGCGGCGAATAATAGGTAAT

Product: selenocysteine-specific translation elongation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MIVVTSGHVDHGKTSLIQALTGTDADRLPEEKQRGMTIELGYAFMDLSDGERLAFVDVPGHSKFINTMLAGVSCAKHALL
IIACDDGVMPQTYEHLAILQLLNLEHLIVVLTKQDKVDATRVDEVKEQVSELLAQHQRQSPIFVVSAQSGFGIDALKLAI
TELAESQITALSETGEQASFRMAIDRAFSVKGAGLVVTGTVISGQVSQGQSLYLSGQRKPVKVRTLHSQGRVSNSAHHCQ
RVALNITGVDQHRGAERGDWLTALAPAAEAPNVICGVFESCLPFKHWQNVQVHLGNRHTTARLGLLQNLEDGTALVELQC
EQPLHSVEQDRIIVRDSAAKSTLGALRVLDINPPTRAKRSVERLAYLENLKQCDSLTASLQVQINHHCVPKSAFMWANQL
TEQTACLADKQVISLDGYLLSSILQEQIEAQLLALIADYHQQSPELLGLSINRLQRMTHNQYPLPVLLKLTDGLREQKLL
QSTRGLLHLPRHNLALSDAELQLWSQIKQQMLSANGPLWSSDLASTLNLELTTVRALCRKLIQLGYLSAIIKDRFMLTES
LYFYADVIRNQINVSGEIKTADFNSIIKLGRKASIQLLEFYDRSGLTWRKKKDNSRVIRDGSIFIASDLAEEHRS

Sequences:

>Translated_635_residues
MIVVTSGHVDHGKTSLIQALTGTDADRLPEEKQRGMTIELGYAFMDLSDGERLAFVDVPGHSKFINTMLAGVSCAKHALL
IIACDDGVMPQTYEHLAILQLLNLEHLIVVLTKQDKVDATRVDEVKEQVSELLAQHQRQSPIFVVSAQSGFGIDALKLAI
TELAESQITALSETGEQASFRMAIDRAFSVKGAGLVVTGTVISGQVSQGQSLYLSGQRKPVKVRTLHSQGRVSNSAHHCQ
RVALNITGVDQHRGAERGDWLTALAPAAEAPNVICGVFESCLPFKHWQNVQVHLGNRHTTARLGLLQNLEDGTALVELQC
EQPLHSVEQDRIIVRDSAAKSTLGALRVLDINPPTRAKRSVERLAYLENLKQCDSLTASLQVQINHHCVPKSAFMWANQL
TEQTACLADKQVISLDGYLLSSILQEQIEAQLLALIADYHQQSPELLGLSINRLQRMTHNQYPLPVLLKLTDGLREQKLL
QSTRGLLHLPRHNLALSDAELQLWSQIKQQMLSANGPLWSSDLASTLNLELTTVRALCRKLIQLGYLSAIIKDRFMLTES
LYFYADVIRNQINVSGEIKTADFNSIIKLGRKASIQLLEFYDRSGLTWRKKKDNSRVIRDGSIFIASDLAEEHRS
>Mature_635_residues
MIVVTSGHVDHGKTSLIQALTGTDADRLPEEKQRGMTIELGYAFMDLSDGERLAFVDVPGHSKFINTMLAGVSCAKHALL
IIACDDGVMPQTYEHLAILQLLNLEHLIVVLTKQDKVDATRVDEVKEQVSELLAQHQRQSPIFVVSAQSGFGIDALKLAI
TELAESQITALSETGEQASFRMAIDRAFSVKGAGLVVTGTVISGQVSQGQSLYLSGQRKPVKVRTLHSQGRVSNSAHHCQ
RVALNITGVDQHRGAERGDWLTALAPAAEAPNVICGVFESCLPFKHWQNVQVHLGNRHTTARLGLLQNLEDGTALVELQC
EQPLHSVEQDRIIVRDSAAKSTLGALRVLDINPPTRAKRSVERLAYLENLKQCDSLTASLQVQINHHCVPKSAFMWANQL
TEQTACLADKQVISLDGYLLSSILQEQIEAQLLALIADYHQQSPELLGLSINRLQRMTHNQYPLPVLLKLTDGLREQKLL
QSTRGLLHLPRHNLALSDAELQLWSQIKQQMLSANGPLWSSDLASTLNLELTTVRALCRKLIQLGYLSAIIKDRFMLTES
LYFYADVIRNQINVSGEIKTADFNSIIKLGRKASIQLLEFYDRSGLTWRKKKDNSRVIRDGSIFIASDLAEEHRS

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=244, Percent_Identity=33.1967213114754, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI54607086, Length=292, Percent_Identity=29.1095890410959, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI4503507, Length=257, Percent_Identity=28.0155642023346, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI53729339, Length=254, Percent_Identity=29.5275590551181, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI53729337, Length=254, Percent_Identity=29.5275590551181, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI45593140, Length=368, Percent_Identity=24.4565217391304, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI2367247, Length=631, Percent_Identity=40.5705229793978, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1790412, Length=235, Percent_Identity=34.8936170212766, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789737, Length=235, Percent_Identity=34.8936170212766, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789559, Length=211, Percent_Identity=32.2274881516588, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789108, Length=93, Percent_Identity=34.4086021505376, Blast_Score=66, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17506081, Length=267, Percent_Identity=31.8352059925094, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI25141371, Length=275, Percent_Identity=29.0909090909091, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17556456, Length=246, Percent_Identity=30.8943089430894, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI115532067, Length=222, Percent_Identity=30.6306306306306, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115532065, Length=222, Percent_Identity=30.6306306306306, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994658, Length=247, Percent_Identity=27.1255060728745, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=246, Percent_Identity=32.1138211382114, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320863, Length=249, Percent_Identity=28.9156626506024, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324550, Length=174, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19921738, Length=240, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281363316, Length=243, Percent_Identity=30.4526748971193, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17864358, Length=243, Percent_Identity=30.4526748971193, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922690, Length=343, Percent_Identity=27.6967930029155, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24647046, Length=257, Percent_Identity=28.7937743190661, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24647048, Length=257, Percent_Identity=28.7937743190661, Blast_Score=93, Evalue=7e-19,
Organism=Drosophila melanogaster, GI28572034, Length=163, Percent_Identity=31.2883435582822, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137380, Length=304, Percent_Identity=26.6447368421053, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 70357; Mature: 70357

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVVTSGHVDHGKTSLIQALTGTDADRLPEEKQRGMTIELGYAFMDLSDGERLAFVDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEEEEEEEECCCCCEEEEEECCC
HSKFINTMLAGVSCAKHALLIIACDDGVMPQTYEHLAILQLLNLEHLIVVLTKQDKVDAT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHH
RVDEVKEQVSELLAQHQRQSPIFVVSAQSGFGIDALKLAITELAESQITALSETGEQASF
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RMAIDRAFSVKGAGLVVTGTVISGQVSQGQSLYLSGQRKPVKVRTLHSQGRVSNSAHHCQ
HHHHHHHHEECCCCEEEEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHE
RVALNITGVDQHRGAERGDWLTALAPAAEAPNVICGVFESCLPFKHWQNVQVHLGNRHTT
EEEEEEECCHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCH
ARLGLLQNLEDGTALVELQCEQPLHSVEQDRIIVRDSAAKSTLGALRVLDINPPTRAKRS
HHHHHHHCCCCCCEEEEEECCCCHHHHCCCCEEEECCCHHHHHCEEEEEECCCCHHHHHH
VERLAYLENLKQCDSLTASLQVQINHHCVPKSAFMWANQLTEQTACLADKQVISLDGYLL
HHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECHHHH
SSILQEQIEAQLLALIADYHQQSPELLGLSINRLQRMTHNQYPLPVLLKLTDGLREQKLL
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
QSTRGLLHLPRHNLALSDAELQLWSQIKQQMLSANGPLWSSDLASTLNLELTTVRALCRK
HHHCCCEECCCCCCEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHH
LIQLGYLSAIIKDRFMLTESLYFYADVIRNQINVSGEIKTADFNSIIKLGRKASIQLLEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCHHHHHHH
YDRSGLTWRKKKDNSRVIRDGSIFIASDLAEEHRS
HCCCCCEEEECCCCCCEEECCCEEEEECHHHHHCC
>Mature Secondary Structure
MIVVTSGHVDHGKTSLIQALTGTDADRLPEEKQRGMTIELGYAFMDLSDGERLAFVDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEEEEEEEECCCCCEEEEEECCC
HSKFINTMLAGVSCAKHALLIIACDDGVMPQTYEHLAILQLLNLEHLIVVLTKQDKVDAT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHH
RVDEVKEQVSELLAQHQRQSPIFVVSAQSGFGIDALKLAITELAESQITALSETGEQASF
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RMAIDRAFSVKGAGLVVTGTVISGQVSQGQSLYLSGQRKPVKVRTLHSQGRVSNSAHHCQ
HHHHHHHHEECCCCEEEEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHE
RVALNITGVDQHRGAERGDWLTALAPAAEAPNVICGVFESCLPFKHWQNVQVHLGNRHTT
EEEEEEECCHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCH
ARLGLLQNLEDGTALVELQCEQPLHSVEQDRIIVRDSAAKSTLGALRVLDINPPTRAKRS
HHHHHHHCCCCCCEEEEEECCCCHHHHCCCCEEEECCCHHHHHCEEEEEECCCCHHHHHH
VERLAYLENLKQCDSLTASLQVQINHHCVPKSAFMWANQLTEQTACLADKQVISLDGYLL
HHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECHHHH
SSILQEQIEAQLLALIADYHQQSPELLGLSINRLQRMTHNQYPLPVLLKLTDGLREQKLL
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHH
QSTRGLLHLPRHNLALSDAELQLWSQIKQQMLSANGPLWSSDLASTLNLELTTVRALCRK
HHHCCCEECCCCCCEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHH
LIQLGYLSAIIKDRFMLTESLYFYADVIRNQINVSGEIKTADFNSIIKLGRKASIQLLEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCHHHHHHH
YDRSGLTWRKKKDNSRVIRDGSIFIASDLAEEHRS
HCCCCCEEEECCCCCCEEECCCEEEEECHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]