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The map label for this gene is dusB [H]

Identifier: 157960219

GI number: 157960219

Start: 471511

End: 472479

Strand: Reverse

Name: dusB [H]

Synonym: Spea_0390

Alternate gene names: 157960219

Gene position: 472479-471511 (Counterclockwise)

Preceding gene: 157960220

Following gene: 157960218

Centisome position: 9.13

GC content: 46.85

Gene sequence:

>969_bases
ATGCAGATTGGACCCTATCGCCTGAAAAATCAGTTGATCGTGGCGCCAATGGCAGGTGTCACTGACCAACCCTTTAGAAA
TCTCTGCTTACGTTATGGCGCAGCCTTAGCCGTGTCGGAGATGCTTTCATCGAATCCTGATGTTTGGGATACCGATAAAA
GCCGCTTGCGTATGACACACGCAGGTGAAGACGGAATACGTTCGGTTCAGATTGCCGGAGCAGATCCAGAGTTAATGGCT
AATGCCGCTGTTGTGAACGTACAACAAGGGGCACAGATCATTGATATCAATATGGGCTGTCCAGCAAAAAAAGTGAATAA
GAAGCTCGCAGGATCGGCTTTGTTGCAAGACCCAGTTCAAGTAGAAGCGATTCTACGTGCCGTGGTAGCAGCGGTTGATG
TTCCTGTGACGCTAAAAATTCGAACGGGATGGGCTCCAGAGCACAGGAATGGTGTTCATATCGCCCAGATCGCAGAAGAT
TGCGGTATTGCCTCATTGGCCGTTCACGGCCGAACTAGGCAGTGCATGTACAAAGGCAACGCCGAGTACGACACAATTAA
AGCGATTAAAAAACAGATCTCGATACCCGTTGTCGCCAATGGAGACATTTTAACCCCAGAGAAAGCACGTTTTGTGCTGG
ATTATACTGGTGTGGATGCCCTGATGATTGGACGAGGAGCTCAAGGACGACCTTGGATATTTAGGGAAATCCAACATTAC
CTGGATACAGGTAATAAGCTAGCGCCCGTAGAGATGGATGAAAAGCGTCAAGTTATGCTTGAACACCTGAAACTACTGTA
CAACTTGTATGGTGATTATAAGGGCATCCGCTTCGCAAGAAAGCATGTTGGCTGGTACTTAGACCAAGAAAATCAACGGC
CTTTTAGAGCCGAGTTTAATCGACTCGAAACCGTTGAAGAACAATGTTCCATGGTGGAACGCTATTTTGATGAATTTGTT
GCATATTAA

Upstream 100 bases:

>100_bases
GTCAAGCAAAAAAGTTGCCCTCAGGTCATTTATTGACCTTTTCACAGGCTTGAAAAAGGCGTACTATAGCGCCCCTTTGA
CGAGCAAGGTGTTTTGATAA

Downstream 100 bases:

>100_bases
TAAAGAGCAGAATACGAATGTTTGATCAGACTACTCACACTGAAGTTCACCCACTTACTGTTGGCAAAATCGAAACCGCA
AGTGGTGCAATCAAGCCACA

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQIGPYRLKNQLIVAPMAGVTDQPFRNLCLRYGAALAVSEMLSSNPDVWDTDKSRLRMTHAGEDGIRSVQIAGADPELMA
NAAVVNVQQGAQIIDINMGCPAKKVNKKLAGSALLQDPVQVEAILRAVVAAVDVPVTLKIRTGWAPEHRNGVHIAQIAED
CGIASLAVHGRTRQCMYKGNAEYDTIKAIKKQISIPVVANGDILTPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
LDTGNKLAPVEMDEKRQVMLEHLKLLYNLYGDYKGIRFARKHVGWYLDQENQRPFRAEFNRLETVEEQCSMVERYFDEFV
AY

Sequences:

>Translated_322_residues
MQIGPYRLKNQLIVAPMAGVTDQPFRNLCLRYGAALAVSEMLSSNPDVWDTDKSRLRMTHAGEDGIRSVQIAGADPELMA
NAAVVNVQQGAQIIDINMGCPAKKVNKKLAGSALLQDPVQVEAILRAVVAAVDVPVTLKIRTGWAPEHRNGVHIAQIAED
CGIASLAVHGRTRQCMYKGNAEYDTIKAIKKQISIPVVANGDILTPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
LDTGNKLAPVEMDEKRQVMLEHLKLLYNLYGDYKGIRFARKHVGWYLDQENQRPFRAEFNRLETVEEQCSMVERYFDEFV
AY
>Mature_322_residues
MQIGPYRLKNQLIVAPMAGVTDQPFRNLCLRYGAALAVSEMLSSNPDVWDTDKSRLRMTHAGEDGIRSVQIAGADPELMA
NAAVVNVQQGAQIIDINMGCPAKKVNKKLAGSALLQDPVQVEAILRAVVAAVDVPVTLKIRTGWAPEHRNGVHIAQIAED
CGIASLAVHGRTRQCMYKGNAEYDTIKAIKKQISIPVVANGDILTPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
LDTGNKLAPVEMDEKRQVMLEHLKLLYNLYGDYKGIRFARKHVGWYLDQENQRPFRAEFNRLETVEEQCSMVERYFDEFV
AY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=236, Percent_Identity=34.7457627118644, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=34.7457627118644, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=32.8888888888889, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI40807366, Length=229, Percent_Identity=34.9344978165939, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI8923374, Length=247, Percent_Identity=33.1983805668016, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=64.6875, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=30.8333333333333, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17543114, Length=245, Percent_Identity=31.4285714285714, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=31.5068493150685, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=264, Percent_Identity=31.4393939393939, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=248, Percent_Identity=30.6451612903226, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=268, Percent_Identity=29.4776119402985, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=31.1688311688312, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585320, Length=239, Percent_Identity=31.7991631799163, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=33.0396475770925, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=33.0396475770925, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=30.6122448979592, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19921524, Length=310, Percent_Identity=23.2258064516129, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36055; Mature: 36055

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPYRLKNQLIVAPMAGVTDQPFRNLCLRYGAALAVSEMLSSNPDVWDTDKSRLRMTH
CCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEE
AGEDGIRSVQIAGADPELMANAAVVNVQQGAQIIDINMGCPAKKVNKKLAGSALLQDPVQ
CCCCCCCEEEEECCCHHHHHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHH
VEAILRAVVAAVDVPVTLKIRTGWAPEHRNGVHIAQIAEDCGIASLAVHGRTRQCMYKGN
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEHHHHHHHCCEEEEEECCCCCEEEECCC
AEYDTIKAIKKQISIPVVANGDILTPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCEECCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGNKLAPVEMDEKRQVMLEHLKLLYNLYGDYKGIRFARKHVGWYLDQENQRPFRAEFN
HCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCEEECCCCCCCHHHHHH
RLETVEEQCSMVERYFDEFVAY
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIGPYRLKNQLIVAPMAGVTDQPFRNLCLRYGAALAVSEMLSSNPDVWDTDKSRLRMTH
CCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEE
AGEDGIRSVQIAGADPELMANAAVVNVQQGAQIIDINMGCPAKKVNKKLAGSALLQDPVQ
CCCCCCCEEEEECCCHHHHHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHH
VEAILRAVVAAVDVPVTLKIRTGWAPEHRNGVHIAQIAEDCGIASLAVHGRTRQCMYKGN
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEHHHHHHHCCEEEEEECCCCCEEEECCC
AEYDTIKAIKKQISIPVVANGDILTPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCEECCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGNKLAPVEMDEKRQVMLEHLKLLYNLYGDYKGIRFARKHVGWYLDQENQRPFRAEFN
HCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCEEECCCCCCCHHHHHH
RLETVEEQCSMVERYFDEFVAY
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]